FastQCFastQC Report
Thu 26 May 2016
SRR1043197_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1043197_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences769391
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT396545.153946432958015No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC50850.6609123319612524No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGCT47800.6212705893362412No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC39870.5182020585112121No Hit
CGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG22610.29386878713164044No Hit
TCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC16950.22030411065375083No Hit
CGGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT14910.1937896336193171No Hit
GCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG14620.19002041874677505No Hit
ACCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG14090.18313185363488785No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCC13690.1779329365693126No Hit
AGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG12100.15726724123365102No Hit
CGTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTC10740.13959092321069522No Hit
ACTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC9280.12061487592134558No Hit
TGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG8970.11658571519552478No Hit
TCCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG8730.11346636495617962No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTT8270.1074876103307681No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCT8030.10436826009142296No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACTT351.2112287E-745.0000048
GCAAACG351.2112287E-745.0000049
AGGGTCG351.2112287E-745.0000045
GCTTAGG1550.045.0000041
GGTATTC351.2112287E-745.0000047
TGCTCGC351.2112287E-745.00000410
AGTTAGG1250.045.0000041
TCGCCGC253.8895985E-545.028
CATGGAT207.031832E-445.029
AAGTAAC207.031832E-445.024
ATCTCGT207.031832E-445.040
AAATCCG207.031832E-445.018
AGATCGT253.8895985E-545.042
ACTGCGA207.031832E-445.016
ATAGGTG453.8380676E-1045.023
CATGCGG1000.045.01
TTAGCGC253.8895985E-545.01
TTTCGTG207.031832E-445.021
TCGGGTA253.8895985E-545.04
GCAGTAC207.031832E-445.037