FastQCFastQC Report
Thu 26 May 2016
SRR805452_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805452_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26218360
Sequences flagged as poor quality0
Sequence length53
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1508510.5753639815762694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1321250.040.0722771
TCAACGC1370900.039.7814912
ATCAACG1373950.039.69831511
CAACGCA1374650.039.6712613
GTGGTAT1376800.039.4932446
AGTGGTA1375300.039.4679685
GGTATCA1380350.039.381468
GTATCAA1384400.039.281539
AACGCAG1391150.039.2277614
ACGCAGA1391400.039.20551315
TGGTATC1391050.039.073477
CGCAGAG1397500.039.02765316
TATCAAC1393200.039.02160310
AGAGTAC1398850.038.65736419
CAGTGGT1436550.037.839164
GCAGTGG1447200.037.784793
CAGAGTA1440400.037.7021318
GTACATG1099500.037.52091622
AGCAGTG1467050.037.2687342
ACATGGG1099350.037.07072424
Produced by FastQC (version 0.11.5)