##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805452_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26218360
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.83790591783773	35.0	35.0	37.0	35.0	37.0
2	36.47943208499692	37.0	37.0	37.0	35.0	37.0
3	38.55066651766167	39.0	39.0	39.0	38.0	39.0
4	38.52432207811625	39.0	39.0	39.0	38.0	39.0
5	38.43042985907585	39.0	39.0	39.0	37.0	39.0
6	38.483046498713115	39.0	39.0	39.0	37.0	39.0
7	38.51131344599739	39.0	39.0	39.0	37.0	39.0
8	39.43817443196294	40.0	40.0	40.0	39.0	40.0
9	39.460192208818555	40.0	40.0	40.0	39.0	40.0
10	39.300063733963526	40.0	40.0	40.0	38.0	40.0
11	39.38567145313437	40.0	40.0	40.0	38.0	40.0
12	39.41249384019443	40.0	40.0	40.0	38.0	40.0
13	39.432185270169455	40.0	40.0	40.0	39.0	40.0
14	39.43526547808482	40.0	40.0	40.0	39.0	40.0
15	39.40974748229866	40.0	40.0	40.0	38.0	40.0
16	39.40068169786363	40.0	40.0	40.0	38.0	40.0
17	39.396547381300735	40.0	40.0	40.0	38.0	40.0
18	39.35676155945681	40.0	40.0	40.0	38.0	40.0
19	39.35519124766004	40.0	40.0	40.0	38.0	40.0
20	39.30056834218464	40.0	40.0	40.0	38.0	40.0
21	39.26439735361022	40.0	40.0	40.0	38.0	40.0
22	39.24743179970067	40.0	40.0	40.0	38.0	40.0
23	39.154189430612746	40.0	40.0	40.0	38.0	40.0
24	39.16156956422903	40.0	40.0	40.0	38.0	40.0
25	39.14723987312708	40.0	40.0	40.0	38.0	40.0
26	39.15115594568081	40.0	40.0	40.0	38.0	40.0
27	39.12921853235672	40.0	40.0	40.0	38.0	40.0
28	39.09897053057476	40.0	40.0	40.0	38.0	40.0
29	39.08676286388622	40.0	40.0	40.0	38.0	40.0
30	39.00234732454661	40.0	40.0	40.0	37.0	40.0
31	38.96117312448224	40.0	40.0	40.0	37.0	40.0
32	38.94653853253979	40.0	40.0	40.0	37.0	40.0
33	38.81808747763018	40.0	40.0	40.0	37.0	40.0
34	38.80606944904258	40.0	39.0	40.0	37.0	40.0
35	38.775297577727976	40.0	39.0	40.0	36.0	40.0
36	38.74228197339574	40.0	39.0	40.0	36.0	40.0
37	38.68178844901054	40.0	39.0	40.0	35.0	40.0
38	38.57061017546483	40.0	39.0	40.0	35.0	40.0
39	38.55542501514206	40.0	39.0	40.0	35.0	40.0
40	34.218425523182994	40.0	36.0	40.0	10.0	40.0
41	35.04907759295394	40.0	36.0	40.0	15.0	40.0
42	36.896548487395854	40.0	35.0	40.0	30.0	40.0
43	37.70675450333278	40.0	37.0	40.0	33.0	40.0
44	37.94245280787967	40.0	38.0	40.0	34.0	40.0
45	38.16689419170383	40.0	38.0	40.0	35.0	40.0
46	38.16975611746883	40.0	38.0	40.0	35.0	40.0
47	38.258021516219934	40.0	38.0	40.0	35.0	40.0
48	38.19382325973097	40.0	38.0	40.0	35.0	40.0
49	38.171804376780244	40.0	38.0	40.0	35.0	40.0
50	38.06605844148909	40.0	38.0	40.0	35.0	40.0
51	37.92653464976452	40.0	37.0	40.0	35.0	40.0
52	37.825617506205575	40.0	37.0	40.0	34.0	40.0
53	37.69531816635365	40.0	37.0	40.0	34.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	8.0
12	31.0
13	73.0
14	204.0
15	483.0
16	1016.0
17	1634.0
18	2375.0
19	3523.0
20	5280.0
21	8235.0
22	12540.0
23	18653.0
24	25911.0
25	35537.0
26	46177.0
27	59107.0
28	74651.0
29	93602.0
30	115660.0
31	143846.0
32	182748.0
33	238474.0
34	336750.0
35	536120.0
36	990469.0
37	2336673.0
38	5196939.0
39	1.575164E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	7.734869763021028	35.27816766571212	44.648692748135275	12.338269823131577
2	33.29142631346888	41.100209929225166	14.745643892295323	10.862719865010625
3	35.00796007072906	25.789538323525957	23.077244343277002	16.12525726246798
4	23.287570237039997	27.32527892667581	21.15169674991113	28.235454086373064
5	20.822095661208408	33.80011945827275	24.48896879896378	20.888816081555063
6	26.543120927472195	26.547026587475344	22.38922648098508	24.520626004067378
7	22.859766972457468	25.380286943958357	29.727584028901884	22.03236205468229
8	24.26324148421183	26.681317977173247	26.233063395269575	22.822377143345353
9	25.832683661373174	25.535891642345288	26.61933469522884	22.012090001052698
10	22.2808062746869	26.880300674794306	29.18781723952223	21.65107581099657
11	21.394953765224063	30.960624539444876	26.410999772678384	21.233421922652678
12	21.40446618323953	26.69565525837619	30.19870426678099	21.70117429160329
13	21.496523810032357	26.66453965846834	26.13152004930896	25.707416482190343
14	21.729379717114266	30.823648008494807	25.619630671025952	21.82734160336497
15	21.587494412312594	30.663348127037693	25.538534828265387	22.21062263238433
16	22.127600658469866	26.187484648162584	25.60889392013841	26.076020773229143
17	26.403352459879258	25.883281791843576	25.47101725660949	22.24234849166767
18	22.17913324860899	26.092394795097785	25.34346923301076	26.385002723282465
19	22.1590938563663	30.40463629304045	25.301239284226778	22.13503056636647
20	26.61525358565524	26.474691780874167	25.252151545710717	21.657903087759873
21	22.588590590715818	30.19831904055021	25.251617568757162	21.96147279997681
22	26.264777812189628	26.128133109774982	25.683780373753358	21.92330870428204
23	22.31048013682015	26.096067793714024	29.83532913576593	21.758122933699898
24	22.066334431291658	30.015561614075022	25.54458402432494	22.37351993030838
25	21.909886812142332	26.330418836265885	25.468690642740434	26.291003708851356
26	22.18396955415976	29.224444244414983	26.556867019905134	22.034719181520128
27	22.38701047662783	25.88274018664783	29.695472943387763	22.03477639333658
28	25.559226435215628	25.834933992820297	26.56283230530056	22.043007266663516
29	25.938887863314104	25.860019467274075	26.287952411973897	21.91314025743792
30	26.060279895462568	25.70877049517971	26.161598208278473	22.069351401079242
31	24.146117453570703	26.641784612004717	26.555585475216603	22.656512459207974
32	23.741622283010834	26.98107356829336	26.90559592590841	22.37170822278739
33	23.65488916926917	26.701216246935356	26.84041641048487	22.803478173310612
34	23.348100338846518	26.680349190414653	27.05370587634009	22.917844594398733
35	23.230114316837515	26.685082514695807	27.174563931535	22.910239236931677
36	23.26063491385426	26.57259264118732	27.127104822727276	23.03966762223114
37	23.268423349133965	26.83233047375961	27.106825140855488	22.792421036250932
38	23.670759727153033	26.710843088583726	27.04859876819145	22.56979841607179
39	23.356777464341782	26.547346973647475	27.181456048356957	22.91441951365379
40	27.52707644566632	27.82490590563254	24.315658950445414	20.332358698255725
41	23.344400641382602	26.651655557403288	27.115544221682818	22.888399579531292
42	23.35570951043467	26.64649123743819	27.133729951072453	22.86406930105468
43	23.26351457528236	26.67890745264006	27.23144773357296	22.82613023850462
44	23.37169449195144	26.685883480126137	27.129973041792088	22.812448986130327
45	23.296396113258037	26.44319858297773	27.176642627532765	23.083762676231466
46	23.194982447414713	26.624102346599866	27.12151713532044	23.059398070664987
47	23.363776376554448	26.666431462532366	27.132826004372507	22.836966156540683
48	23.3941672934539	26.44813786979811	27.04273646406564	23.11495837268235
49	23.074238815852706	26.75156264541337	27.06687222236631	23.10732631636761
50	23.46614738679307	26.610390581256798	27.03640120892382	22.887060823026307
51	23.429848396314643	26.482442074942902	26.98004756971832	23.107661959024135
52	23.30837245350205	26.595252334623527	27.037572144100547	23.05880306777388
53	23.396020956306955	26.667747334310764	26.878336402429444	23.05789530695284
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	368.0
1	359.5
2	351.0
3	418.0
4	485.0
5	533.5
6	582.0
7	718.5
8	1210.0
9	1565.0
10	2314.0
11	3063.0
12	5050.0
13	7037.0
14	11314.5
15	15592.0
16	24807.5
17	34023.0
18	51540.0
19	69057.0
20	91346.0
21	113635.0
22	243411.0
23	373187.0
24	338836.5
25	336075.0
26	367664.0
27	415152.0
28	462640.0
29	523090.5
30	583541.0
31	651214.5
32	718888.0
33	790770.0
34	862652.0
35	950706.5
36	1038761.0
37	1128903.5
38	1219046.0
39	1313933.0
40	1408820.0
41	1492877.5
42	1661747.0
43	1746559.0
44	1797345.5
45	1848132.0
46	1883254.5
47	1918377.0
48	1929698.0
49	1941019.0
50	1910882.5
51	1880746.0
52	1810713.0
53	1740680.0
54	1639810.5
55	1538941.0
56	1435915.5
57	1332890.0
58	1178414.0
59	885725.0
60	747512.0
61	636777.5
62	526043.0
63	438321.5
64	350600.0
65	282704.5
66	214809.0
67	168260.0
68	121711.0
69	92256.0
70	62801.0
71	47134.0
72	31467.0
73	23235.0
74	15003.0
75	10891.5
76	4953.5
77	3127.0
78	2441.5
79	1756.0
80	1402.5
81	1049.0
82	838.5
83	628.0
84	471.5
85	315.0
86	220.5
87	126.0
88	91.0
89	56.0
90	39.5
91	23.0
92	23.0
93	14.0
94	5.0
95	6.5
96	8.0
97	5.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	2.621836E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.41558895455194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.23175926177743	32.550652211096065
2	17.33008427908051	16.78092474007409
3	6.290079291751972	9.13613680443008
4	2.956023490679453	5.7247047305894485
5	1.6482353066904982	3.990014155455351
6	1.0087484035526648	2.9303488838979783
7	0.7130384043816365	2.416552200674563
8	0.4944419755985174	1.9150959561963536
9	0.3657660081862366	1.59378990353029
>10	1.8289278199411512	15.530270144775335
>50	0.09618226311348352	3.1708329590921354
>100	0.03554639020374129	2.7788116759832224
>500	8.603722282979954E-4	0.2710736192404157
>1k	2.077547376919283E-4	0.18523421465277884
>5k	5.3988041908851605E-5	0.16812801466183344
>10k+	4.4990034923848963E-5	0.8574297856503306
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	150851	0.5753639815762694	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.004149763753339263	0.0	0.0	0.0	0.0
2	0.03362147746846103	3.8141210968191756E-6	0.0	1.9070605484095877E-5	0.0
3	0.03371683049588151	3.8141210968191756E-6	0.0	1.9070605484095877E-5	0.0
4	0.03503651639538095	3.8141210968191756E-6	0.0	1.9070605484095877E-5	0.0
5	0.035040330516477765	7.628242193638351E-6	0.0	2.669884767773423E-5	3.8141210968191756E-6
6	0.03513186942280143	7.628242193638351E-6	0.0	2.669884767773423E-5	3.8141210968191756E-6
7	0.035150940028285525	7.628242193638351E-6	0.0	2.669884767773423E-5	3.8141210968191756E-6
8	0.035181452997060075	1.1442363290457526E-5	0.0	3.432708987137258E-5	3.8141210968191756E-6
9	0.03525392129789964	1.1442363290457526E-5	0.0	3.8141210968191755E-5	3.8141210968191756E-6
10	0.03538360141519149	1.5256484387276703E-5	0.0	3.8141210968191755E-5	3.8141210968191756E-6
11	0.03543699911054696	1.9070605484095877E-5	0.0	3.8141210968191755E-5	3.8141210968191756E-6
12	0.03562007692319428	2.2884726580915052E-5	0.0	4.5769453161830104E-5	3.8141210968191756E-6
13	0.03573450055609886	2.2884726580915052E-5	0.0	4.5769453161830104E-5	3.8141210968191756E-6
14	0.036077771454812585	2.2884726580915052E-5	0.0	4.958357425864928E-5	1.1442363290457526E-5
15	0.03676812737333685	2.2884726580915052E-5	0.0	4.958357425864928E-5	1.1442363290457526E-5
16	0.03785896600702714	2.2884726580915052E-5	0.0	5.339769535546846E-5	1.1442363290457526E-5
17	0.03805348618296491	2.2884726580915052E-5	0.0	5.339769535546846E-5	1.5256484387276703E-5
18	0.038133582725998115	2.2884726580915052E-5	0.0	5.339769535546846E-5	1.5256484387276703E-5
19	0.03818316630025677	2.669884767773423E-5	0.0	5.339769535546846E-5	1.5256484387276703E-5
20	0.038289961690967704	2.669884767773423E-5	0.0	6.102593754910681E-5	1.5256484387276703E-5
21	0.038362429991807265	3.0512968774553405E-5	0.0	6.484005864592599E-5	1.5256484387276703E-5
22	0.03845015477703411	3.432708987137258E-5	0.0	7.246830083956433E-5	1.5256484387276703E-5
23	0.03862179022639097	3.432708987137258E-5	0.0	7.246830083956433E-5	1.5256484387276703E-5
24	0.03910618360568701	3.432708987137258E-5	0.0	8.772478522684104E-5	1.5256484387276703E-5
25	0.039399870930142085	4.195533206501093E-5	0.0	8.772478522684104E-5	1.5256484387276703E-5
26	0.03963634643814487	4.195533206501093E-5	0.0	8.772478522684104E-5	1.5256484387276703E-5
27	0.04000250206343951	4.195533206501093E-5	0.0	1.0298126961411774E-4	1.5256484387276703E-5
28	0.04134507268951986	4.195533206501093E-5	0.0	1.0298126961411774E-4	1.5256484387276703E-5
29	0.041623503529587665	4.5769453161830104E-5	0.0	1.0679539071093692E-4	1.5256484387276703E-5
30	0.04225664763165965	6.102593754910681E-5	0.0	1.0679539071093692E-4	1.9070605484095877E-5
31	0.05642610750634288	6.484005864592599E-5	0.0	1.0679539071093692E-4	1.9070605484095877E-5
32	0.056731237194088416	6.865417974274517E-5	0.0	1.0679539071093692E-4	1.9070605484095877E-5
33	0.056887616159058005	6.865417974274517E-5	0.0	1.1060951180775609E-4	1.9070605484095877E-5
34	0.05695245621770393	6.865417974274517E-5	0.0	1.1442363290457526E-4	2.2884726580915052E-5
35	0.0570058539130594	7.628242193638351E-5	0.0	1.1442363290457526E-4	2.2884726580915052E-5
36	0.05706306572951168	1.0679539071093692E-4	0.0	1.1442363290457526E-4	2.2884726580915052E-5
37	0.057146976393641705	1.0679539071093692E-4	0.0	1.1442363290457526E-4	2.2884726580915052E-5
38	0.05724995766325582	1.1060951180775609E-4	0.0	1.1442363290457526E-4	2.2884726580915052E-5
39	0.05734912481177312	1.1060951180775609E-4	0.0	1.1442363290457526E-4	2.669884767773423E-5
40	0.05745592020248406	1.1060951180775609E-4	0.0	1.1442363290457526E-4	2.669884767773423E-5
41	0.0575817861986791	1.1442363290457526E-4	0.0	1.1823775400139444E-4	2.669884767773423E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	132125	0.0	40.072277	1
TCAACGC	137090	0.0	39.78149	12
ATCAACG	137395	0.0	39.698315	11
CAACGCA	137465	0.0	39.67126	13
GTGGTAT	137680	0.0	39.493244	6
AGTGGTA	137530	0.0	39.467968	5
GGTATCA	138035	0.0	39.38146	8
GTATCAA	138440	0.0	39.28153	9
AACGCAG	139115	0.0	39.22776	14
ACGCAGA	139140	0.0	39.205513	15
TGGTATC	139105	0.0	39.07347	7
CGCAGAG	139750	0.0	39.027653	16
TATCAAC	139320	0.0	39.021603	10
AGAGTAC	139885	0.0	38.657364	19
CAGTGGT	143655	0.0	37.83916	4
GCAGTGG	144720	0.0	37.78479	3
CAGAGTA	144040	0.0	37.70213	18
GTACATG	109950	0.0	37.520916	22
AGCAGTG	146705	0.0	37.268734	2
ACATGGG	109935	0.0	37.070724	24
>>END_MODULE