FastQCFastQC Report
Thu 26 May 2016
SRR805451_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805451_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39980347
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6195581.5496563849233225No Hit
CAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTC2231140.5580591884307557Illumina Paired End PCR Primer 2 (100% over 52bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG523100.13083928461151173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT5075200.044.4458921
GTGGTAT5160850.043.8330276
AGTGGTA5159600.043.748465
ATCAACG5194500.043.69022411
TCAACGC5194700.043.66366612
CAACGCA5202200.043.6083913
GTATCAA5191250.043.5949029
GGTATCA5189350.043.5855068
TGGTATC5190750.043.555647
TATCAAC5204050.043.47593310
AACGCAG5229250.043.41202514
ACGCAGA5229650.043.39252515
CGCAGAG5243200.043.27007716
AGAGTAC5202350.043.1644519
AGCAGTG5249650.043.1148532
GCAGTGG5259750.043.0928233
CAGTGGT5261200.042.925514
CAGAGTA5278200.042.73694618
GTACATG4087000.042.47307222
GCAGAGT5341650.042.38679517