##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805451_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39980347
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.76401365400856	37.0	37.0	37.0	37.0	37.0
2	36.66951817601783	37.0	37.0	37.0	37.0	37.0
3	38.59224708579943	39.0	39.0	39.0	38.0	39.0
4	38.5708777114916	39.0	39.0	39.0	38.0	39.0
5	38.46979227068739	39.0	39.0	39.0	38.0	39.0
6	38.51033088832371	39.0	39.0	39.0	38.0	39.0
7	38.49048028522614	39.0	39.0	39.0	37.0	39.0
8	39.43352560196639	40.0	40.0	40.0	39.0	40.0
9	39.47422817515816	40.0	40.0	40.0	39.0	40.0
10	39.161649272328724	40.0	40.0	40.0	38.0	40.0
11	39.357460529294556	40.0	40.0	40.0	38.0	40.0
12	39.43110686357975	40.0	40.0	40.0	38.0	40.0
13	39.46258062742677	40.0	40.0	40.0	39.0	40.0
14	39.46942408979092	40.0	40.0	40.0	39.0	40.0
15	39.456553165984275	40.0	40.0	40.0	39.0	40.0
16	39.443628140596175	40.0	40.0	40.0	39.0	40.0
17	39.4250065913635	40.0	40.0	40.0	39.0	40.0
18	39.397082646631354	40.0	40.0	40.0	39.0	40.0
19	39.39752293795749	40.0	40.0	40.0	38.0	40.0
20	39.35611061604843	40.0	40.0	40.0	38.0	40.0
21	39.30129003132464	40.0	40.0	40.0	38.0	40.0
22	39.31105710513218	40.0	40.0	40.0	38.0	40.0
23	39.02313759307792	40.0	39.0	40.0	37.0	40.0
24	39.148313345054255	40.0	40.0	40.0	38.0	40.0
25	39.1912517917866	40.0	40.0	40.0	38.0	40.0
26	39.20883145411419	40.0	40.0	40.0	38.0	40.0
27	39.206127300495915	40.0	40.0	40.0	38.0	40.0
28	39.18340798792967	40.0	40.0	40.0	38.0	40.0
29	39.174983248644644	40.0	40.0	40.0	38.0	40.0
30	39.09667637451971	40.0	40.0	40.0	38.0	40.0
31	39.01606436782552	40.0	40.0	40.0	37.0	40.0
32	38.984440980464726	40.0	40.0	40.0	37.0	40.0
33	38.82110580480955	40.0	40.0	40.0	37.0	40.0
34	38.819900412570206	40.0	40.0	40.0	37.0	40.0
35	38.81099776347614	40.0	40.0	40.0	36.0	40.0
36	38.76541771886072	40.0	39.0	40.0	36.0	40.0
37	38.70969826750128	40.0	39.0	40.0	36.0	40.0
38	38.56565687136232	40.0	39.0	40.0	35.0	40.0
39	38.548284360813575	40.0	39.0	40.0	35.0	40.0
40	34.21849465188484	40.0	35.0	40.0	10.0	40.0
41	35.072679809407354	40.0	35.0	40.0	15.0	40.0
42	36.93255686350096	40.0	35.0	40.0	30.0	40.0
43	37.749665729514554	40.0	37.0	40.0	34.0	40.0
44	37.98014009233086	40.0	38.0	40.0	35.0	40.0
45	38.157569792978535	40.0	38.0	40.0	35.0	40.0
46	38.16459149291526	40.0	38.0	40.0	35.0	40.0
47	38.22683502471852	40.0	38.0	40.0	35.0	40.0
48	38.148446085272845	40.0	38.0	40.0	35.0	40.0
49	38.12089217234658	40.0	38.0	40.0	35.0	40.0
50	37.99839941359188	40.0	38.0	40.0	35.0	40.0
51	37.828286808015946	40.0	37.0	40.0	35.0	40.0
52	37.674847244322315	40.0	37.0	40.0	34.0	40.0
53	37.51893601623818	40.0	37.0	40.0	34.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	9.0
12	19.0
13	108.0
14	275.0
15	773.0
16	1706.0
17	2911.0
18	4249.0
19	5820.0
20	7988.0
21	12456.0
22	18683.0
23	27579.0
24	38902.0
25	53873.0
26	70915.0
27	91765.0
28	117012.0
29	144563.0
30	178110.0
31	219157.0
32	274368.0
33	357295.0
34	502009.0
35	809445.0
36	1522626.0
37	3528926.0
38	7624023.0
39	2.4364781E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.550003480460036	35.64682417588822	34.7968615680099	19.00631077564184
2	26.715621052513626	43.4265940713321	14.054002582819002	15.803782293335273
3	36.618288980833505	25.022361611818926	19.118225762272647	19.241123645074918
4	21.049824805172403	24.229607111714163	19.87983495991168	34.84073312320176
5	20.282677886712687	36.097693199111056	23.13621490078613	20.483414013390128
6	33.015426304328976	23.48199479109073	20.940553617506122	22.56202528707417
7	22.16156102897256	22.513954168531853	34.73387812266862	20.590606679826966
8	32.970621790751345	23.980264603506317	22.576702498355004	20.472411107387337
9	32.41519389514053	24.100871360621255	23.016793726177514	20.4671410180607
10	20.465540231554268	24.56462921644977	34.51726669605944	20.452563855936518
11	20.711353505761217	35.72957233212608	23.27026826455508	20.28880589755762
12	19.919657025488046	24.43143127297019	35.03599656101034	20.61291514053142
13	20.68235425770567	23.727275303538512	23.25036848729702	32.3400019514588
14	20.397861979537097	35.75337152526465	22.759704912015895	21.089061583182357
15	20.824434065067017	35.675818421485936	22.778881834117147	20.720865679329897
16	21.135591944712235	23.456299666433612	22.947139503316468	32.46096888553769
17	32.51639611832283	23.162460295804838	23.55211924498804	20.769024340884286
18	20.474519643363777	23.453183135203904	23.533022362212115	32.53927485922021
19	20.512370740554104	35.384225154424	22.837358064951264	21.266046040070638
20	32.693343056777366	24.297305373562665	22.79257356120496	20.216778008455005
21	21.45114948602122	35.292262470858496	22.81289854737879	20.443689495741495
22	32.36682513035717	23.358374053131655	23.126937842735583	21.14786297377559
23	20.6674719456537	24.080121165531654	34.882226009694214	20.370180879120433
24	21.08958183879695	35.15820660586063	23.019139878901	20.733071676441426
25	21.12947644001189	23.54900021252942	22.936326690711315	32.385196656747375
26	20.655868744710997	33.23295568195043	25.55808232479823	20.55309324854034
27	20.810402170846594	23.949777124245568	34.79732429535942	20.442496409548422
28	30.081847463705103	23.12642008834991	26.29550213758775	20.496230310357237
29	31.001864491071075	23.16376843852806	25.36548019455659	20.468886875844277
30	30.24379203111969	23.140859682883693	25.34222126686394	21.273127019132676
31	25.968506476444542	24.545117129673738	26.445868516348796	23.040507877532928
32	24.88062197159019	26.33801302424914	27.139039088380095	21.64232591578057
33	24.01866597105823	26.393507790215025	27.219708723388518	22.368117515338223
34	24.100741296717608	25.407568373531124	27.64521778662902	22.84647254312225
35	23.068306535708658	26.189322468862013	27.782652811892806	22.959718183536527
36	22.949463144979706	25.73014936563707	27.78844815929186	23.531939330091355
37	23.416820269218775	25.668063861476742	27.75890614456148	23.15620972474301
38	24.408752630386125	25.537074503130253	27.679359561336476	22.37481330514715
39	23.36199833383137	26.139948210054303	27.874983176109	22.623070280005322
40	27.45420143552031	26.915714363359577	25.426962902548095	20.20312129857202
41	23.30298684000917	25.810964071922637	27.571328983212677	23.314720104855517
42	23.337751420716785	25.842634632460793	28.369263528403092	22.45035041841933
43	23.26786458356652	25.506249357965803	28.045979690971667	23.17990636749601
44	23.937493589037633	25.604865310448655	27.78389092020637	22.67375018030734
45	23.203550484441767	25.356515790120582	28.54219099198914	22.89774273344851
46	23.143143304884273	26.033453386485117	27.77852078172308	23.044882526907536
47	23.31513781008454	25.535276119539436	28.36977878155985	22.77980728881618
48	23.828875222118505	25.494388530444724	27.71271094770638	22.96402529973039
49	22.83614997138469	25.832544675012453	27.651325787642612	23.679979565960245
50	23.46316804103776	25.574730504465105	27.446500151687026	23.5156013028101
51	23.90224627114917	25.526892000212005	27.583657540541108	22.987204188097717
52	23.34696844927334	25.422918415390445	28.20990022923013	23.020212906106092
53	23.55283209522919	25.574600440561458	27.200094086226915	23.672473377982435
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	570.0
1	458.5
2	347.0
3	358.0
4	369.0
5	431.0
6	493.0
7	583.5
8	933.0
9	1192.0
10	1889.0
11	2586.0
12	4464.5
13	6343.0
14	10379.0
15	14415.0
16	23860.5
17	33306.0
18	51198.0
19	69090.0
20	96916.5
21	124743.0
22	529907.5
23	935072.0
24	698558.5
25	465263.5
26	468482.0
27	515660.5
28	562839.0
29	630125.5
30	697412.0
31	781229.5
32	865047.0
33	957621.5
34	1050196.0
35	1171755.5
36	1293315.0
37	1422824.0
38	1552333.0
39	1698580.0
40	1844827.0
41	1991868.5
42	2298924.0
43	2458938.0
44	2600350.0
45	2741762.0
46	2856637.0
47	2971512.0
48	3056520.5
49	3141529.0
50	3114148.0
51	3086767.0
52	2985521.5
53	2884276.0
54	2735309.0
55	2586342.0
56	2453214.0
57	2320086.0
58	2159359.5
59	1653778.0
60	1308923.0
61	1115220.0
62	921517.0
63	766744.0
64	611971.0
65	494536.5
66	377102.0
67	297802.0
68	218502.0
69	167795.5
70	117089.0
71	87827.0
72	58565.0
73	42762.0
74	26959.0
75	19921.5
76	9354.5
77	5825.0
78	4297.5
79	2770.0
80	2331.0
81	1892.0
82	1433.5
83	975.0
84	726.0
85	477.0
86	331.5
87	186.0
88	147.5
89	109.0
90	82.0
91	55.0
92	49.0
93	36.0
94	29.0
95	22.5
96	16.0
97	10.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	3.9980347E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.76808929241751
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.21412099864213	29.642796812861476
2	16.52977057124135	14.800124898330157
3	6.6099928173372815	8.877502460063814
4	3.3777185880837597	6.048560294239686
5	1.9757446204103988	4.422515579277313
6	1.2217988500437766	3.281856000967969
7	0.8251167764103238	2.5857231066106343
8	0.5876506927311367	2.104639894795082
9	0.42399978426099244	1.7083494181585681
>10	2.0811095809864164	16.355044779339767
>50	0.11359536063719018	3.444733162694146
>100	0.03741411036738688	2.7181829459876297
>500	0.0012868186780711915	0.39552935716898685
>1k	5.646536180023568E-4	0.49101011550772955
>5k	4.265452121809023E-5	0.140761952273209
>10k+	7.312203055801102E-5	2.9826692217237745
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	619558	1.5496563849233225	No Hit
CAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCTCGTATGCCGTC	223114	0.5580591884307557	Illumina Paired End PCR Primer 2 (100% over 52bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	52310	0.13083928461151173	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.023669129234921347	0.0	0.0	0.0	0.0
2	0.7118147323733833	2.5012289162973E-6	0.0	2.5012289162973E-6	0.0
3	0.712307474469894	2.5012289162973E-6	0.0	1.25061445814865E-5	0.0
4	0.7154140007839351	2.5012289162973E-6	0.0	1.5007373497783798E-5	0.0
5	0.7155415634586664	2.5012289162973E-6	0.0	1.5007373497783798E-5	0.0
6	0.716186880519071	2.5012289162973E-6	0.0	2.25110602466757E-5	2.5012289162973E-6
7	0.7166471066396697	5.0024578325946E-6	0.0	2.5012289162973E-5	7.503686748891899E-6
8	0.7176576031218539	5.0024578325946E-6	0.0	2.7513518079270296E-5	7.503686748891899E-6
9	0.7209717314359477	5.0024578325946E-6	0.0	4.00196626607568E-5	1.00049156651892E-5
10	0.725263840256314	5.0024578325946E-6	0.0	4.00196626607568E-5	1.00049156651892E-5
11	0.7303413349563974	5.0024578325946E-6	0.0	4.00196626607568E-5	1.25061445814865E-5
12	0.736849532596603	5.0024578325946E-6	0.0	4.7523349409648695E-5	1.25061445814865E-5
13	0.7452386543818642	7.503686748891899E-6	0.0	5.0024578325946E-5	1.25061445814865E-5
14	0.7586702536623807	1.00049156651892E-5	0.0	5.7528265074837896E-5	1.25061445814865E-5
15	0.7786100505831027	1.25061445814865E-5	0.0	6.50319518237298E-5	1.25061445814865E-5
16	0.7946029082739077	1.5007373497783798E-5	0.0	6.50319518237298E-5	1.25061445814865E-5
17	0.8029244968784288	1.5007373497783798E-5	0.0	7.00344096563244E-5	1.25061445814865E-5
18	0.8094101834583877	1.75086024140811E-5	0.0	7.25356385726217E-5	1.5007373497783798E-5
19	0.8148453538935018	2.00098313303784E-5	0.0	7.753809640521629E-5	1.5007373497783798E-5
20	0.819272529075348	2.25110602466757E-5	0.0	7.753809640521629E-5	2.25110602466757E-5
21	0.8226842053171775	2.25110602466757E-5	0.0	8.754301207040549E-5	2.25110602466757E-5
22	0.8249278076550961	2.5012289162973E-5	0.0	9.00442409867028E-5	2.25110602466757E-5
23	0.8262784712698967	2.5012289162973E-5	0.0	9.504669881929739E-5	2.25110602466757E-5
24	0.8272764616074993	2.5012289162973E-5	0.0	9.754792773559469E-5	2.25110602466757E-5
25	0.8280293315113048	2.5012289162973E-5	0.0	1.0255038556818929E-4	2.25110602466757E-5
26	0.8289672923549163	2.5012289162973E-5	0.0	1.075528434007839E-4	2.25110602466757E-5
27	0.8297251647165543	3.0014746995567596E-5	0.0	1.1005407231708118E-4	2.25110602466757E-5
28	0.8307881870059807	3.0014746995567596E-5	0.0	1.1255530123337849E-4	2.7513518079270296E-5
29	0.8312434106687469	3.25159759118649E-5	0.0	1.300639036474596E-4	2.7513518079270296E-5
30	0.8320112879460501	3.50172048281622E-5	0.0	1.325651325637569E-4	2.7513518079270296E-5
31	0.8436420024068325	3.7518433744459494E-5	0.0	1.450712771452434E-4	2.7513518079270296E-5
32	0.8441322432744268	3.7518433744459494E-5	0.0	1.575774217267299E-4	2.7513518079270296E-5
33	0.8444599042624618	3.7518433744459494E-5	0.0	1.575774217267299E-4	3.0014746995567596E-5
34	0.8446850148649285	4.2520891577054094E-5	0.0	1.575774217267299E-4	3.25159759118649E-5
35	0.8449376389854746	5.0024578325946E-5	0.0	1.650811084756218E-4	3.50172048281622E-5
36	0.8452227790819324	7.503686748891899E-5	0.0	1.7008356630821638E-4	3.50172048281622E-5
37	0.845993157588152	7.503686748891899E-5	0.0	1.725847952245137E-4	3.7518433744459494E-5
38	0.846245781708698	7.503686748891899E-5	0.0	1.725847952245137E-4	3.7518433744459494E-5
39	0.8465759439256493	7.503686748891899E-5	0.0	1.7758725305710827E-4	3.7518433744459494E-5
40	0.8467835459257019	7.753809640521629E-5	0.0	1.8258971088970288E-4	4.00196626607568E-5
41	0.847061182335411	7.753809640521629E-5	0.0	1.9759708438748668E-4	4.2520891577054094E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	507520	0.0	44.445892	1
GTGGTAT	516085	0.0	43.833027	6
AGTGGTA	515960	0.0	43.74846	5
ATCAACG	519450	0.0	43.690224	11
TCAACGC	519470	0.0	43.663666	12
CAACGCA	520220	0.0	43.60839	13
GTATCAA	519125	0.0	43.594902	9
GGTATCA	518935	0.0	43.585506	8
TGGTATC	519075	0.0	43.55564	7
TATCAAC	520405	0.0	43.475933	10
AACGCAG	522925	0.0	43.412025	14
ACGCAGA	522965	0.0	43.392525	15
CGCAGAG	524320	0.0	43.270077	16
AGAGTAC	520235	0.0	43.16445	19
AGCAGTG	524965	0.0	43.114853	2
GCAGTGG	525975	0.0	43.092823	3
CAGTGGT	526120	0.0	42.92551	4
CAGAGTA	527820	0.0	42.736946	18
GTACATG	408700	0.0	42.473072	22
GCAGAGT	534165	0.0	42.386795	17
>>END_MODULE