FastQCFastQC Report
Thu 26 May 2016
SRR805450_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805450_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45087930
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCCG143450.022.72185521
ATGCCGA163200.020.15931122
GAGATCG117750.019.67813127
GATCGGA117700.019.60662729
AGATCGG117850.019.54178828
CGAGATC118750.019.41347326
GCCGAGA167600.019.09725424
ACCGATC46300.018.57667230
AGCGGTT198300.018.2738277
AAGAGCG222950.018.1823274
TGCCGAG186050.017.80972723
GCGGTTC205450.017.4319788
GACCGAT49250.017.36852829
CGGTTCA202150.017.077179
GAGCGGT228650.016.9376776
AGAGCGG235450.016.7678895
CCGAGAT138400.016.65715225
CTCGTAT45000.016.55444336
CCGATCT56250.015.54133231
TATGCCG47700.015.518867540
Produced by FastQC (version 0.11.5)