##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805450_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	45087930
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.749237146171936	37.0	37.0	37.0	37.0	37.0
2	36.6360734458202	37.0	37.0	37.0	37.0	37.0
3	38.56308943435638	39.0	39.0	39.0	38.0	39.0
4	38.55374076831649	39.0	39.0	39.0	38.0	39.0
5	38.475646209528804	39.0	39.0	39.0	38.0	39.0
6	38.4897629365553	39.0	39.0	39.0	38.0	39.0
7	38.53259231461724	39.0	39.0	39.0	38.0	39.0
8	39.444562280858754	40.0	40.0	40.0	39.0	40.0
9	39.45175130018167	40.0	40.0	40.0	39.0	40.0
10	39.38305089632635	40.0	40.0	40.0	39.0	40.0
11	39.420133503578455	40.0	40.0	40.0	38.0	40.0
12	39.41147617555297	40.0	40.0	40.0	38.0	40.0
13	39.434312974669716	40.0	40.0	40.0	39.0	40.0
14	39.440993787029036	40.0	40.0	40.0	39.0	40.0
15	39.41912713668603	40.0	40.0	40.0	39.0	40.0
16	39.41624556727266	40.0	40.0	40.0	39.0	40.0
17	39.4131450700886	40.0	40.0	40.0	39.0	40.0
18	39.367482450403024	40.0	40.0	40.0	38.0	40.0
19	39.37255409596315	40.0	40.0	40.0	38.0	40.0
20	39.315523311893	40.0	40.0	40.0	38.0	40.0
21	39.291226144114404	40.0	40.0	40.0	38.0	40.0
22	39.269437408193276	40.0	40.0	40.0	38.0	40.0
23	39.28622593674183	40.0	40.0	40.0	38.0	40.0
24	39.244476049355114	40.0	40.0	40.0	38.0	40.0
25	39.18887209947319	40.0	40.0	40.0	38.0	40.0
26	39.18681549585443	40.0	40.0	40.0	38.0	40.0
27	39.163244486939185	40.0	40.0	40.0	38.0	40.0
28	39.12809250724085	40.0	40.0	40.0	38.0	40.0
29	39.11709220183761	40.0	40.0	40.0	38.0	40.0
30	39.02845848101698	40.0	40.0	40.0	38.0	40.0
31	39.019507837241584	40.0	40.0	40.0	38.0	40.0
32	39.01543044890284	40.0	40.0	40.0	37.0	40.0
33	38.90856983232541	40.0	40.0	40.0	37.0	40.0
34	38.90163671297396	40.0	40.0	40.0	37.0	40.0
35	38.875774181693416	40.0	40.0	40.0	37.0	40.0
36	38.85699795932082	40.0	40.0	40.0	37.0	40.0
37	38.84357345746412	40.0	40.0	40.0	37.0	40.0
38	38.74002980398524	40.0	39.0	40.0	36.0	40.0
39	38.73615564076683	40.0	39.0	40.0	36.0	40.0
40	34.35429413148929	40.0	37.0	40.0	10.0	40.0
41	35.23698355635311	40.0	37.0	40.0	15.0	40.0
42	37.13494955301785	40.0	37.0	40.0	30.0	40.0
43	37.947748521611	40.0	37.0	40.0	34.0	40.0
44	38.18437770374466	40.0	38.0	40.0	35.0	40.0
45	38.39209580036165	40.0	39.0	40.0	35.0	40.0
46	38.40070923637435	40.0	39.0	40.0	35.0	40.0
47	38.469311143802784	40.0	39.0	40.0	35.0	40.0
48	38.41145071419336	40.0	39.0	40.0	35.0	40.0
49	38.41105699463249	40.0	39.0	40.0	35.0	40.0
50	38.326988043141476	40.0	38.0	40.0	35.0	40.0
51	38.22055907201772	40.0	38.0	40.0	35.0	40.0
52	38.16924939778784	40.0	38.0	40.0	35.0	40.0
53	38.089187993327705	40.0	38.0	40.0	35.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	6.0
12	19.0
13	83.0
14	300.0
15	787.0
16	1723.0
17	3065.0
18	4511.0
19	6572.0
20	9869.0
21	14782.0
22	22414.0
23	32381.0
24	46014.0
25	62267.0
26	81880.0
27	104473.0
28	131501.0
29	163840.0
30	201399.0
31	249127.0
32	306883.0
33	390525.0
34	521755.0
35	778145.0
36	1359840.0
37	3171168.0
38	8357541.0
39	2.9065058E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.360027838936054	22.687754793799584	36.86188299174524	27.090334375519127
2	27.384271134203765	25.0561602628464	28.37595560497011	19.183612997979726
3	19.002112095188224	25.07630534380265	31.40788898492346	24.513693576085664
4	19.29562301928698	28.391103783207612	29.609292331672798	22.703980865832605
5	24.3628904675819	28.027602952719278	27.65405508747019	19.95545149222863
6	23.13223516803721	26.949862191500028	27.575546271474426	22.34235636898833
7	23.612492301154656	27.251275452210827	28.211878433984438	20.924353812650082
8	21.874712367589286	25.919207202459727	28.281151962398805	23.92492846755218
9	21.514487358368413	25.18776976454674	29.898400747162267	23.399342129922577
10	22.845329115796623	26.39191907900851	27.47542191446802	23.287329890726856
11	22.483540495205702	26.891888804830916	27.61899914234253	23.00557155762085
12	22.44366951421367	26.805941190912957	27.53361708998395	23.216772204889423
13	22.64453258333217	26.80115055182174	27.120843649287067	23.43347321555902
14	22.915208127762796	26.995885595102724	26.714322436181924	23.374583840952557
15	22.686200053983406	26.962200305048377	26.77673381767582	23.5748658232924
16	23.2463810159393	26.616611141828866	26.568192418680564	23.56881542355127
17	23.65381599909333	26.33033940569017	26.397971696638102	23.617872898578398
18	23.128573434176285	26.73767458386313	26.373051501809908	23.760700480150675
19	23.53127544333927	26.596137813379322	26.47521853409549	23.397368209185917
20	23.681155910240278	26.513100069131585	26.448628269250772	23.357115751377364
21	23.2683824695434	26.874562660117686	26.56022354541448	23.29683132492443
22	23.470383315446064	26.45101471724251	26.64679882176893	23.4318031455425
23	23.307468318017705	26.49397743475915	26.95050316126733	23.24805108595582
24	23.244890594888698	26.56847187262755	26.580663605537	23.605973926946746
25	23.2659938036632	26.395895753031912	26.495299296286166	23.842811147018725
26	23.371662881839995	26.398038233292148	26.793285919313657	23.437012965554196
27	23.53063669146044	26.15982592236991	26.917425572653258	23.39211181351639
28	23.699945861342492	26.32537355340997	26.559387401461986	23.41529318378555
29	23.931187348809317	26.21367625437673	26.535689263179744	23.31944713363421
30	23.743094881490457	26.318427126727705	26.500384914543652	23.438093077238186
31	23.539013656204666	26.253502877599395	26.55839600531672	23.64908746087922
32	23.60331246078496	26.397441621294217	26.416916899933085	23.582329017987743
33	23.68392383504854	26.310963932032365	26.423464106691082	23.581648126228018
34	23.603130593930572	26.383369118963767	26.374124959828492	23.63937532727717
35	23.562257127350932	26.46878887542631	26.45032495392891	23.51862904329385
36	23.50060426371315	26.376034561799578	26.43704201989313	23.686319154594145
37	23.579090900824234	26.366530909713532	26.385079110972715	23.66929907848952
38	23.82411878300911	26.392140867855325	26.289918388358036	23.49382196077753
39	23.61107950620044	26.268409305993867	26.45297533064836	23.667535857157336
40	27.95800117681162	27.425188958552766	23.67560453540449	20.941205329231128
41	23.773353533861503	26.160001135558897	26.424280289647363	23.64236504093224
42	23.56132561419431	26.227549146745037	26.596326333899118	23.614798905161535
43	23.569216861364005	26.23546700857635	26.53417000957906	23.66114612048058
44	23.740149525604746	26.232827721299245	26.445986764085198	23.581035989010807
45	23.496408018731398	26.20406170786727	26.484977243355374	23.814553030045957
46	23.571574476805655	26.273991731268215	26.43050146680054	23.723932325125592
47	23.699369210340773	26.236766691218694	26.4300933753224	23.63377072311814
48	23.609236440883404	26.199342041207036	26.37372130412729	23.81770021378227
49	23.582887925881714	26.315000489044404	26.375495614901816	23.72661597017206
50	23.90125472604309	26.222982514389102	26.34403486698103	23.531727892586773
51	23.70942289876692	26.15809818725322	26.4030351360109	23.72944377796896
52	23.604317164261033	26.292568765077483	26.357339979901496	23.745774090759987
53	23.674173997342525	26.297421505045808	26.36969583655759	23.658708661054078
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	801.0
1	692.5
2	584.0
3	606.5
4	629.0
5	736.0
6	843.0
7	1016.5
8	1585.0
9	1980.0
10	2895.5
11	3811.0
12	6431.5
13	9052.0
14	14751.0
15	20450.0
16	33683.5
17	46917.0
18	72531.0
19	98145.0
20	131868.5
21	165592.0
22	228217.5
23	290843.0
24	342934.0
25	467951.5
26	540878.0
27	625370.5
28	709863.0
29	817365.5
30	924868.0
31	1043495.5
32	1162123.0
33	1290505.0
34	1418887.0
35	1576522.0
36	1734157.0
37	1895974.0
38	2057791.0
39	2229851.5
40	2401912.0
41	2557794.5
42	2862212.5
43	3010748.0
44	3107388.0
45	3204028.0
46	3269301.0
47	3334574.0
48	3351664.0
49	3368754.0
50	3345043.0
51	3321332.0
52	3219194.0
53	3117056.0
54	2952559.5
55	2788063.0
56	2593920.5
57	2399778.0
58	2175854.0
59	1678487.0
60	1405044.0
61	1196972.0
62	988900.0
63	821710.5
64	654521.0
65	526552.0
66	398583.0
67	311605.0
68	224627.0
69	170897.0
70	117167.0
71	87060.5
72	56954.0
73	40831.0
74	24708.0
75	18091.5
76	8151.0
77	4827.0
78	3493.0
79	2159.0
80	1662.0
81	1165.0
82	883.5
83	602.0
84	506.5
85	411.0
86	321.0
87	231.0
88	169.5
89	108.0
90	94.0
91	80.0
92	62.5
93	36.0
94	27.0
95	19.0
96	11.0
97	7.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	4.508793E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.33148218623595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.8871220258364	27.683236899924125
2	17.461586032468833	15.132728082186997
3	6.895085588800481	8.96322835091041
4	3.5446949441420292	6.1438754331092404
5	2.0702596250990384	4.485370903293126
6	1.3705099526065057	3.563173655845673
7	0.9473884763249195	2.873622281972293
8	0.6432376451942642	2.2297952451401293
9	0.4960949271139386	1.9346875647227688
>10	2.4941140933761314	18.880067041561006
>50	0.1369181449120986	4.024574413485759
>100	0.05202691526986339	3.6124997867625317
>500	8.223761058179326E-4	0.2245395605917006
>1k	1.167836402260583E-4	0.08616205029933294
>5k	5.61727741146226E-6	0.018126367080016532
>10k+	1.6851832082412702E-5	0.1443123631146902
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.562999676410072E-4	0.0	0.0	8.871553872621785E-6	2.2178884681554463E-6
2	0.0018497189824416423	0.0	0.0	2.2178884681554465E-5	2.2178884681554463E-6
3	0.0019051661941455285	0.0	0.0	2.2178884681554465E-5	6.65366540446634E-6
4	0.0019783565135946582	0.0	0.0	2.8832550086020803E-5	6.65366540446634E-6
5	0.0058241751173762025	0.0	0.0	3.32683270223317E-5	6.65366540446634E-6
6	0.0081440864550668	8.871553872621785E-6	0.0	3.32683270223317E-5	6.65366540446634E-6
7	0.008452372952140406	8.871553872621785E-6	0.0	3.32683270223317E-5	6.65366540446634E-6
8	0.008554395821675557	8.871553872621785E-6	0.0	4.435776936310893E-5	6.65366540446634E-6
9	0.008711865902914593	8.871553872621785E-6	0.0	4.879354629941982E-5	6.65366540446634E-6
10	0.008920347418921205	8.871553872621785E-6	0.0	5.5447211703886165E-5	6.65366540446634E-6
11	0.009233069692931124	1.1089442340777232E-5	0.0	5.5447211703886165E-5	8.871553872621785E-6
12	0.0097121336020527	1.1089442340777232E-5	0.0	5.988298864019705E-5	1.1089442340777232E-5
13	0.01055049544301546	1.9960996213399017E-5	0.0	6.21008771083525E-5	1.5525219277088127E-5
14	0.011107185448522476	2.439677314970991E-5	0.0	6.65366540446634E-5	1.9960996213399017E-5
15	0.012400214425457102	2.439677314970991E-5	0.0	7.319031944912973E-5	1.9960996213399017E-5
16	0.013484761886385114	2.661466161786536E-5	0.0	7.984398485359607E-5	1.9960996213399017E-5
17	0.01549416883853395	2.661466161786536E-5	0.0	8.427976178990696E-5	1.9960996213399017E-5
18	0.01658536996486643	2.661466161786536E-5	2.2178884681554463E-6	9.758709259883965E-5	1.9960996213399017E-5
19	0.01759450921787716	2.8832550086020803E-5	2.2178884681554463E-6	1.0202286953515054E-4	1.9960996213399017E-5
20	0.01884983409085314	2.8832550086020803E-5	2.2178884681554463E-6	1.1311231187592778E-4	1.9960996213399017E-5
21	0.02018500294868272	3.32683270223317E-5	4.4357769363108926E-6	1.1533020034408321E-4	2.2178884681554465E-5
22	0.022023632488783584	3.548621549048714E-5	4.4357769363108926E-6	1.197659772803941E-4	2.2178884681554465E-5
23	0.024671791319761188	3.770410395864259E-5	4.4357769363108926E-6	1.2198386574854955E-4	2.2178884681554465E-5
24	0.028746052435762743	3.9921992426798035E-5	4.4357769363108926E-6	1.330733080893268E-4	2.2178884681554465E-5
25	0.035213415208904024	3.9921992426798035E-5	4.4357769363108926E-6	1.3972697349379313E-4	2.2178884681554465E-5
26	0.04616313057618746	3.9921992426798035E-5	4.4357769363108926E-6	1.3972697349379313E-4	2.2178884681554465E-5
27	0.07079056412658553	4.435776936310893E-5	4.4357769363108926E-6	1.4416275043010402E-4	2.2178884681554465E-5
28	0.17190853516672866	4.6575657831264376E-5	4.4357769363108926E-6	1.4416275043010402E-4	2.2178884681554465E-5
29	0.17357860518324972	5.101143476757527E-5	4.4357769363108926E-6	1.530343043027258E-4	2.439677314970991E-5
30	0.17479400806379888	5.5447211703886165E-5	4.4357769363108926E-6	1.6412374664350303E-4	2.439677314970991E-5
31	0.18323750946206668	5.5447211703886165E-5	4.4357769363108926E-6	1.7521318898428028E-4	2.439677314970991E-5
32	0.18343711942420066	5.7665100172041605E-5	4.4357769363108926E-6	1.7521318898428028E-4	2.661466161786536E-5
33	0.18353914229373583	5.7665100172041605E-5	4.4357769363108926E-6	1.7521318898428028E-4	2.661466161786536E-5
34	0.18367886926722962	5.7665100172041605E-5	4.4357769363108926E-6	1.863026313250575E-4	2.661466161786536E-5
35	0.18387404345242728	5.7665100172041605E-5	4.4357769363108926E-6	1.907384082613684E-4	3.32683270223317E-5
36	0.18402042409132555	9.09334271943733E-5	4.4357769363108926E-6	1.907384082613684E-4	3.770410395864259E-5
37	0.1841534973994149	9.315131566252875E-5	4.4357769363108926E-6	1.9295629672952383E-4	3.770410395864259E-5
38	0.18429322437290868	9.758709259883965E-5	4.4357769363108926E-6	2.0404573907030108E-4	3.770410395864259E-5
39	0.184455130231084	9.98049810669951E-5	4.4357769363108926E-6	2.1291729294292287E-4	4.213988089495348E-5
40	0.18459707509304596	9.98049810669951E-5	4.4357769363108926E-6	2.1735306987923376E-4	4.213988089495348E-5
41	0.18482551760526597	1.0202286953515054E-4	4.4357769363108926E-6	2.1735306987923376E-4	4.213988089495348E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCCG	14345	0.0	22.721855	21
ATGCCGA	16320	0.0	20.159311	22
GAGATCG	11775	0.0	19.678131	27
GATCGGA	11770	0.0	19.606627	29
AGATCGG	11785	0.0	19.541788	28
CGAGATC	11875	0.0	19.413473	26
GCCGAGA	16760	0.0	19.097254	24
ACCGATC	4630	0.0	18.576672	30
AGCGGTT	19830	0.0	18.273827	7
AAGAGCG	22295	0.0	18.182327	4
TGCCGAG	18605	0.0	17.809727	23
GCGGTTC	20545	0.0	17.431978	8
GACCGAT	4925	0.0	17.368528	29
CGGTTCA	20215	0.0	17.07717	9
GAGCGGT	22865	0.0	16.937677	6
AGAGCGG	23545	0.0	16.767889	5
CCGAGAT	13840	0.0	16.657152	25
CTCGTAT	4500	0.0	16.554443	36
CCGATCT	5625	0.0	15.541332	31
TATGCCG	4770	0.0	15.5188675	40
>>END_MODULE