FastQCFastQC Report
Thu 26 May 2016
SRR805449_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805449_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50675822
Sequences flagged as poor quality0
Sequence length53
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9437581.8623437425445217No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG1137060.22437919211256208No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG856700.16905497852605134No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC560320.11056949406760487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT7911050.045.157381
AGTGGTA8128800.043.9326935
AGCAGTG8149200.043.9080772
GTGGTAT8168400.043.8281756
GCAGTGG8196350.043.723733
ATCAACG8220450.043.69074211
TCAACGC8218800.043.6689212
TGGTATC8198650.043.6449667
GGTATCA8206100.043.6282548
GTATCAA8211950.043.6140569
CAACGCA8230750.043.6083713
TATCAAC8220150.043.5711210
AACGCAG8263800.043.46638514
ACGCAGA8266200.043.4364215
CGCAGAG8287650.043.32116716
CAGTGGT8257550.043.2605134
AGAGTAC8227850.043.22187819
CAGAGTA8311150.042.94843718
GAGTACT1760450.042.7510620
GCAGAGT8397550.042.64759417