##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805449_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	50675822
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.77075495687075	37.0	37.0	37.0	37.0	37.0
2	36.66458629916255	37.0	37.0	37.0	37.0	37.0
3	38.58967479600035	39.0	39.0	39.0	38.0	39.0
4	38.56608802517303	39.0	39.0	39.0	38.0	39.0
5	38.46728526672937	39.0	39.0	39.0	38.0	39.0
6	38.50726318756112	39.0	39.0	39.0	38.0	39.0
7	38.47709525067003	39.0	39.0	39.0	37.0	39.0
8	39.420696422053105	40.0	40.0	40.0	39.0	40.0
9	39.46488260220031	40.0	40.0	40.0	39.0	40.0
10	39.1078578458974	40.0	39.0	40.0	38.0	40.0
11	39.340723787371424	40.0	40.0	40.0	38.0	40.0
12	39.42950004836626	40.0	40.0	40.0	38.0	40.0
13	39.46259717306608	40.0	40.0	40.0	39.0	40.0
14	39.47359330451512	40.0	40.0	40.0	39.0	40.0
15	39.45588558977889	40.0	40.0	40.0	39.0	40.0
16	39.44091458447384	40.0	40.0	40.0	39.0	40.0
17	39.43360326745169	40.0	40.0	40.0	39.0	40.0
18	39.39882660413481	40.0	40.0	40.0	39.0	40.0
19	39.396588199398124	40.0	40.0	40.0	39.0	40.0
20	39.35046638217334	40.0	40.0	40.0	38.0	40.0
21	39.29203573648988	40.0	40.0	40.0	38.0	40.0
22	39.30860608437688	40.0	40.0	40.0	38.0	40.0
23	38.95707967006435	40.0	39.0	40.0	37.0	40.0
24	39.1187504368454	40.0	40.0	40.0	38.0	40.0
25	39.196675527039304	40.0	40.0	40.0	38.0	40.0
26	39.20534356601063	40.0	40.0	40.0	38.0	40.0
27	39.20845394476285	40.0	40.0	40.0	38.0	40.0
28	39.18560780326366	40.0	40.0	40.0	38.0	40.0
29	39.17426768134121	40.0	40.0	40.0	38.0	40.0
30	39.08987751594834	40.0	40.0	40.0	38.0	40.0
31	38.99308161197661	40.0	40.0	40.0	37.0	40.0
32	38.95554136250617	40.0	40.0	40.0	37.0	40.0
33	38.78119597152267	40.0	40.0	40.0	36.0	40.0
34	38.78260993970655	40.0	40.0	40.0	36.0	40.0
35	38.77174333748351	40.0	40.0	40.0	36.0	40.0
36	38.73660121388855	40.0	39.0	40.0	36.0	40.0
37	38.67079438790356	40.0	39.0	40.0	36.0	40.0
38	38.49867376596279	40.0	39.0	40.0	35.0	40.0
39	38.48413831353342	40.0	39.0	40.0	35.0	40.0
40	34.13773288176756	40.0	35.0	40.0	10.0	40.0
41	35.00362397673589	40.0	35.0	40.0	15.0	40.0
42	36.880160345499675	40.0	35.0	40.0	30.0	40.0
43	37.70500517978771	40.0	37.0	40.0	34.0	40.0
44	37.938110446437356	40.0	38.0	40.0	35.0	40.0
45	38.13118322974613	40.0	38.0	40.0	35.0	40.0
46	38.13046803661123	40.0	38.0	40.0	35.0	40.0
47	38.17023923163989	40.0	38.0	40.0	35.0	40.0
48	38.08371785266749	40.0	38.0	40.0	35.0	40.0
49	38.04770075559899	40.0	38.0	40.0	35.0	40.0
50	37.9191804920303	40.0	38.0	40.0	35.0	40.0
51	37.73937642294189	40.0	37.0	40.0	34.0	40.0
52	37.59404605217849	40.0	37.0	40.0	34.0	40.0
53	37.41272234321132	40.0	37.0	40.0	34.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	6.0
12	17.0
13	69.0
14	225.0
15	740.0
16	1613.0
17	2924.0
18	4628.0
19	6984.0
20	10901.0
21	16716.0
22	25657.0
23	37944.0
24	53959.0
25	75038.0
26	98718.0
27	127370.0
28	161118.0
29	197090.0
30	238116.0
31	290946.0
32	359120.0
33	462366.0
34	648260.0
35	1051505.0
36	1986992.0
37	4635602.0
38	9405196.0
39	3.0776E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.399068731435674	37.79000368262403	30.226580241757105	19.58434734418319
2	23.566483045899087	41.12168718249898	17.850214644766886	17.461615126835042
3	36.94501886915618	23.194242414064835	20.067842214774533	19.79289650200445
4	20.205211471458718	22.93236210356884	20.352392113146188	36.51003431182626
5	20.88324882031514	38.0870467182555	21.645566992480163	19.3841374689492
6	36.144106355097705	22.745667549309807	20.66127511459015	20.44895098100234
7	21.020408904269967	22.2485290914472	37.32801018994817	19.403051814334653
8	35.08242846065723	23.258444628683083	21.968835552386302	19.690291358273377
9	34.700319217318274	22.78595895296972	22.689384298492485	19.824337531219523
10	19.740149454309787	23.209689622794873	37.11757847756273	19.93258244533261
11	19.48844164777436	37.97971348150998	22.655589089408355	19.876255781307307
12	19.358582876070564	23.158402837550423	37.330309116643434	20.15270516973558
13	19.452160045869608	23.15673734902613	22.496529015355684	34.89457358974858
14	19.86789084546078	38.084887897822355	22.18107088623052	19.866150370486345
15	19.615275308213057	37.93373889426007	22.2603749772426	20.190610820284277
16	19.85400453889036	22.93435713780824	22.37848652953276	34.833151793768636
17	34.961903528668955	22.59183482016335	22.299279131574817	20.146982519592875
18	19.912584743075307	22.975765444909804	22.274474403197644	34.837175408817245
19	20.115474397238195	37.737665113749905	22.257103200023078	19.88975728898882
20	35.01935498944644	22.935625987477813	22.337228195331495	19.707790827744244
21	20.13720073450412	37.761408191859225	22.316241855928848	19.785149217707808
22	34.7868693674076	22.741428841548935	22.606348644921834	19.865353146121638
23	20.040937865793275	22.871980251252758	37.29810440963345	19.788977473320514
24	19.818796427219276	37.56813850991899	22.51481781588072	20.098247246981018
25	19.910281869724777	22.960450449131343	22.45227319647622	34.67699448466766
26	20.154485111262723	34.2616820305352	25.71782851396076	19.866004344241322
27	20.421878899172075	22.57798798014564	37.1971548877885	19.802978232893786
28	32.00432545524372	22.494824060278688	25.631850628885704	19.868999855591884
29	32.14572227363179	22.53532660999559	25.477056889180798	19.841894227191816
30	32.03180206923925	22.525536931596292	25.470752107385646	19.971908891778806
31	26.769430597494797	24.25004768546231	26.792068612128283	22.188453104914608
32	24.71797497433786	26.329915279913962	27.706522451673305	21.245587294074873
33	24.3776943568868	25.732336418736335	27.740489340261714	22.14947988411515
34	23.56725659033217	25.30832948304223	28.29543248454855	22.82898144207705
35	22.964831236481967	25.448818570718007	28.565397518366847	23.020952674433186
36	22.963647239900716	25.787946764829982	28.53898847462208	22.709417520647225
37	23.756922976010138	25.680702327828055	28.349665447952678	22.212709248209137
38	23.88530964529791	25.59176445129987	28.361452133919013	22.161473769483205
39	23.512477015173033	25.56697945619905	28.53659088154505	22.383952647082864
40	27.558361066151033	27.115323358740977	25.316607592472796	20.00970798263519
41	23.367062501719264	26.05857089007851	28.166428163710894	22.40793844449134
42	23.452472068435316	26.06031333838058	28.30306728916997	22.184147304014132
43	23.23977300259678	25.58826574140228	28.7967188771008	22.37524237890014
44	23.271855363293366	25.599937974365766	28.55911838983095	22.569088272509916
45	23.39037539440406	25.264813662026043	28.568793615227396	22.776017328342498
46	23.243246059235112	25.35747126114698	28.364287805731102	23.034994873886802
47	23.204043932429947	25.51746077251594	28.439576175005115	22.838919120049006
48	23.01939966558411	25.502747641666275	28.44443648097114	23.03341621177847
49	22.713097776687274	26.08991917289472	28.259585014723587	22.937398035694418
50	23.513392639195867	25.74865189162595	28.005978867002888	22.731976602175294
51	23.302473514884475	25.539279856180723	28.242115145167258	22.916131483767547
52	23.48664023644254	25.512320254025678	28.130572800575393	22.870466708956393
53	23.796389528718446	25.61903781254895	27.726464900756813	22.858107757975784
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	559.0
1	447.5
2	336.0
3	385.0
4	434.0
5	520.0
6	606.0
7	781.5
8	1357.0
9	1757.0
10	2664.0
11	3571.0
12	6117.5
13	8664.0
14	13665.5
15	18667.0
16	30891.0
17	43115.0
18	67314.0
19	91513.0
20	128962.0
21	166411.0
22	768670.5
23	1370930.0
24	1009477.5
25	637167.0
26	626309.0
27	678039.0
28	729769.0
29	807534.0
30	885299.0
31	986100.0
32	1086901.0
33	1199335.0
34	1311769.0
35	1458955.5
36	1606142.0
37	1766705.0
38	1927268.0
39	2116368.5
40	2305469.0
41	2492539.0
42	2890493.0
43	3101377.0
44	3288800.0
45	3476223.0
46	3630453.0
47	3784683.0
48	3906566.0
49	4028449.0
50	3987734.5
51	3947020.0
52	3819847.5
53	3692675.0
54	3499155.0
55	3305635.0
56	3176135.5
57	3046636.0
58	2653211.0
59	1933701.5
60	1607617.0
61	1382044.5
62	1156472.0
63	959408.5
64	762345.0
65	611722.5
66	461100.0
67	362993.5
68	264887.0
69	202486.0
70	140085.0
71	104537.0
72	68989.0
73	49767.0
74	30545.0
75	22485.5
76	10334.0
77	6242.0
78	4637.0
79	3032.0
80	2443.0
81	1854.0
82	1395.0
83	936.0
84	650.0
85	364.0
86	265.5
87	167.0
88	130.5
89	94.0
90	75.0
91	56.0
92	37.5
93	18.0
94	17.0
95	11.5
96	6.0
97	5.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	5.0675822E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.33483767725724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.66695871765854	23.639928524915632
2	17.667821209823416	13.545861163787665
3	7.179847158332289	8.257148260865549
4	3.7402597490031777	5.735290013952628
5	2.235823501442735	4.285496550140207
6	1.4777304348934566	3.3989133811430046
7	1.1118427324756197	2.9835617470463722
8	0.824427230739783	2.5283427253696225
9	0.6570604148996656	2.2669473914396976
>10	3.220809064023389	20.846141030412756
>50	0.15482550668346198	4.044431380339056
>100	0.058927225481259754	3.777130633919914
>500	0.0025428352895515895	0.6671470158184174
>1k	9.193532259731259E-4	0.5962410721520902
>5k	1.0520159631057164E-4	0.27130272376698
>10k+	9.966443159932313E-5	3.1561163849303955
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	943758	1.8623437425445217	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	113706	0.22437919211256208	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	85670	0.16905497852605134	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCC	56032	0.11056949406760487	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.012384604239868077	1.9733276354155635E-6	0.0	3.946655270831127E-6	0.0
2	0.017055470752896717	1.9733276354155635E-6	0.0	7.893310541662254E-6	0.0
3	0.017126510547771676	1.9733276354155635E-6	0.0	7.893310541662254E-6	0.0
4	0.0172054436531883	1.9733276354155635E-6	0.0	7.893310541662254E-6	1.9733276354155635E-6
5	0.017262670154615353	3.946655270831127E-6	0.0	7.893310541662254E-6	5.919982906246691E-6
6	0.018126987658927368	3.946655270831127E-6	0.0	7.893310541662254E-6	5.919982906246691E-6
7	0.019040638354124773	3.946655270831127E-6	0.0	1.1839965812493383E-5	5.919982906246691E-6
8	0.020802819932550873	3.946655270831127E-6	0.0	1.3813293447908945E-5	5.919982906246691E-6
9	0.02687474906672456	3.946655270831127E-6	0.0	1.7759948718740074E-5	5.919982906246691E-6
10	0.03401424845165807	3.946655270831127E-6	0.0	1.7759948718740074E-5	5.919982906246691E-6
11	0.04335795480535076	3.946655270831127E-6	0.0	1.9733276354155636E-5	7.893310541662254E-6
12	0.05372779152945955	3.946655270831127E-6	0.0	1.9733276354155636E-5	7.893310541662254E-6
13	0.07126475422539767	3.946655270831127E-6	0.0	2.3679931624986765E-5	7.893310541662254E-6
14	0.0996076590528714	3.946655270831127E-6	0.0	2.9599914531233456E-5	9.866638177077818E-6
15	0.14704448208062615	3.946655270831127E-6	0.0	2.9599914531233456E-5	9.866638177077818E-6
16	0.1851277321165111	3.946655270831127E-6	0.0	3.354656980206458E-5	9.866638177077818E-6
17	0.20183195055030384	3.946655270831127E-6	0.0	3.551989743748015E-5	9.866638177077818E-6
18	0.21080467130853842	3.946655270831127E-6	0.0	3.551989743748015E-5	1.1839965812493383E-5
19	0.217521878579493	5.919982906246691E-6	0.0	3.749322507289571E-5	1.1839965812493383E-5
20	0.22086074893861612	5.919982906246691E-6	0.0	3.749322507289571E-5	1.1839965812493383E-5
21	0.22255978403270893	5.919982906246691E-6	0.0	4.735986324997353E-5	1.5786621083324508E-5
22	0.22404175308690602	5.919982906246691E-6	0.0	4.933319088538909E-5	1.7759948718740074E-5
23	0.22553161545164477	5.919982906246691E-6	0.0	5.525317379163578E-5	1.9733276354155636E-5
24	0.2267787585172274	5.919982906246691E-6	0.0	5.919982906246691E-5	1.9733276354155636E-5
25	0.22793315518394552	5.919982906246691E-6	0.0	5.919982906246691E-5	1.9733276354155636E-5
26	0.2293381644603614	5.919982906246691E-6	0.0	6.314648433329803E-5	2.3679931624986765E-5
27	0.23076685366840227	7.893310541662254E-6	0.0	6.906646723954472E-5	2.3679931624986765E-5
28	0.23414124392496288	7.893310541662254E-6	0.0	7.10397948749603E-5	2.3679931624986765E-5
29	0.2353351071443893	7.893310541662254E-6	0.0	8.485308832286924E-5	2.3679931624986765E-5
30	0.2380484326430857	9.866638177077818E-6	0.0	8.485308832286924E-5	3.1573242166649016E-5
31	0.2826633971521962	9.866638177077818E-6	0.0	9.471972649994706E-5	3.1573242166649016E-5
32	0.2836046744342894	9.866638177077818E-6	0.0	9.866638177077818E-5	3.1573242166649016E-5
33	0.2842381126052578	9.866638177077818E-6	0.0	1.0063970940619375E-4	3.1573242166649016E-5
34	0.28461501818362217	9.866638177077818E-6	0.0	1.0063970940619375E-4	3.1573242166649016E-5
35	0.2850313903146948	1.3813293447908945E-5	0.0	1.0458636467702487E-4	3.1573242166649016E-5
36	0.2854556557563092	2.17066039895712E-5	0.0	1.08533019947856E-4	3.354656980206458E-5
37	0.2867777852720376	2.17066039895712E-5	0.0	1.08533019947856E-4	3.551989743748015E-5
38	0.28720797069655823	2.17066039895712E-5	0.0	1.1050634758327156E-4	3.551989743748015E-5
39	0.28763026281053716	2.17066039895712E-5	0.0	1.1050634758327156E-4	3.551989743748015E-5
40	0.28799730175072447	2.17066039895712E-5	0.0	1.1050634758327156E-4	3.551989743748015E-5
41	0.2884590604174117	2.17066039895712E-5	0.0	1.1247967521868713E-4	3.946655270831127E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	791105	0.0	45.15738	1
AGTGGTA	812880	0.0	43.932693	5
AGCAGTG	814920	0.0	43.908077	2
GTGGTAT	816840	0.0	43.828175	6
GCAGTGG	819635	0.0	43.72373	3
ATCAACG	822045	0.0	43.690742	11
TCAACGC	821880	0.0	43.66892	12
TGGTATC	819865	0.0	43.644966	7
GGTATCA	820610	0.0	43.628254	8
GTATCAA	821195	0.0	43.614056	9
CAACGCA	823075	0.0	43.60837	13
TATCAAC	822015	0.0	43.57112	10
AACGCAG	826380	0.0	43.466385	14
ACGCAGA	826620	0.0	43.43642	15
CGCAGAG	828765	0.0	43.321167	16
CAGTGGT	825755	0.0	43.260513	4
AGAGTAC	822785	0.0	43.221878	19
CAGAGTA	831115	0.0	42.948437	18
GAGTACT	176045	0.0	42.75106	20
GCAGAGT	839755	0.0	42.647594	17
>>END_MODULE