FastQCFastQC Report
Thu 26 May 2016
SRR805448_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805448_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21108538
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT931830.44144696330934907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT366550.033.5520361
TCAACGC396350.030.89314812
CAACGCA396100.030.83312213
AACGCAG404550.030.17797514
ATCAACG406800.030.1161511
ACGCAGA405700.030.11461615
GGTATCA411850.029.8342868
GTGGTAT414300.029.543816
GTATCAA416800.029.5285519
AGTGGTA415700.029.401015
CGCAGAG416300.029.27215816
TGGTATC422000.029.0420637
TATCAAC425500.028.77144410
AGAGTAC426700.028.34778619
AGCAGTG436750.028.1797372
GCAGTGG441550.027.8020613
GAGTACT216250.027.33294720
CAGAGTA448800.027.22760618
CAGTGGT456100.026.915154
GCAGAGT459550.026.62985417
Produced by FastQC (version 0.11.5)