##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805448_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	21108538
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.25140315260109	37.0	37.0	37.0	35.0	37.0
2	36.17500302484236	37.0	37.0	37.0	35.0	37.0
3	37.873826031911825	39.0	38.0	39.0	35.0	39.0
4	37.859765560267604	39.0	38.0	39.0	35.0	39.0
5	37.89920216170348	39.0	38.0	39.0	35.0	39.0
6	37.83667623025337	39.0	38.0	39.0	35.0	39.0
7	37.78491461606673	39.0	38.0	39.0	35.0	39.0
8	38.73468849429553	40.0	39.0	40.0	36.0	40.0
9	38.71105166070715	40.0	39.0	40.0	36.0	40.0
10	38.70410390335891	40.0	39.0	40.0	36.0	40.0
11	38.78289874931177	40.0	39.0	40.0	36.0	40.0
12	38.75169658836628	40.0	39.0	40.0	36.0	40.0
13	38.840488479116836	40.0	39.0	40.0	36.0	40.0
14	38.77454217814611	40.0	39.0	40.0	36.0	40.0
15	38.76029922110191	40.0	39.0	40.0	36.0	40.0
16	38.56709365660473	40.0	39.0	40.0	36.0	40.0
17	38.37395962714234	40.0	39.0	40.0	35.0	40.0
18	38.53489123690139	40.0	39.0	40.0	35.0	40.0
19	38.48473612904883	40.0	39.0	40.0	35.0	40.0
20	38.39889901422827	40.0	39.0	40.0	35.0	40.0
21	38.40068620574291	40.0	39.0	40.0	35.0	40.0
22	38.22860806371337	40.0	38.0	40.0	35.0	40.0
23	38.222280576703135	40.0	38.0	40.0	34.0	40.0
24	38.227311621487004	40.0	38.0	40.0	35.0	40.0
25	38.08682519841024	40.0	38.0	40.0	34.0	40.0
26	38.038521000364874	40.0	38.0	40.0	34.0	40.0
27	37.61202604367958	40.0	38.0	40.0	33.0	40.0
28	37.797387341558185	40.0	38.0	40.0	33.0	40.0
29	37.839379875574515	40.0	38.0	40.0	34.0	40.0
30	37.7597433796694	40.0	38.0	40.0	33.0	40.0
31	37.69646448276048	40.0	38.0	40.0	33.0	40.0
32	37.61879287897627	40.0	38.0	40.0	33.0	40.0
33	37.4746639961517	40.0	38.0	40.0	33.0	40.0
34	37.27943579038965	40.0	37.0	40.0	32.0	40.0
35	37.295309556730075	40.0	37.0	40.0	32.0	40.0
36	37.22007454992856	40.0	37.0	40.0	32.0	40.0
37	36.91614701122361	40.0	37.0	40.0	31.0	40.0
38	36.92049027744129	40.0	36.0	40.0	31.0	40.0
39	36.86747836349443	39.0	36.0	40.0	31.0	40.0
40	36.73992391135757	39.0	36.0	40.0	31.0	40.0
41	36.871726976070065	40.0	36.0	40.0	31.0	40.0
42	36.84727075840117	40.0	36.0	40.0	31.0	40.0
43	36.78489855621455	39.0	36.0	40.0	31.0	40.0
44	36.57919610538636	39.0	36.0	40.0	30.0	40.0
45	36.52684686168222	39.0	36.0	40.0	30.0	40.0
46	36.360586555070746	39.0	35.0	40.0	30.0	40.0
47	36.1991862723984	39.0	35.0	40.0	29.0	40.0
48	31.273087932475477	38.0	30.0	40.0	3.0	40.0
49	32.52113770266799	38.0	30.0	40.0	12.0	40.0
50	34.069920332710865	38.0	30.0	40.0	22.0	40.0
51	34.51562846275758	37.0	33.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	8.0
8	62.0
9	182.0
10	332.0
11	408.0
12	446.0
13	523.0
14	592.0
15	808.0
16	1366.0
17	2406.0
18	4784.0
19	8888.0
20	15157.0
21	24018.0
22	35858.0
23	50952.0
24	68373.0
25	88167.0
26	111595.0
27	139173.0
28	170509.0
29	210659.0
30	258390.0
31	318476.0
32	396938.0
33	509453.0
34	687919.0
35	993929.0
36	1617471.0
37	2978251.0
38	4403349.0
39	8009096.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	20.402156700762504	30.433633063549927	21.781115300358557	27.383094935329012
2	23.38936026739512	24.822031729530487	30.227995894362746	21.56061210871165
3	20.998190400491023	26.486263520476882	27.60811288778029	24.907433191251805
4	21.130137009015026	28.00175455069413	27.61499635834561	23.25311208194523
5	24.36070655390724	28.556501639289277	26.09459262408415	20.988199182719335
6	24.1016502421911	26.28245499522516	27.613627244103782	22.00226751847996
7	23.09082703880297	27.14479799595784	28.027701397415587	21.736673567823598
8	22.92442044067666	26.07819641511885	28.151798101791798	22.845585042412694
9	22.800878014384512	25.934548380375748	28.857654660877035	22.4069189443627
10	22.223467110796587	26.680805653143764	28.75653917860157	22.339188057458077
11	22.050589197603358	28.053809316400784	27.47018765581965	22.425413830176204
12	22.129533556516325	26.69611225561903	28.333463928198157	22.84089025966649
13	22.381474264110572	27.01654657466093	26.72765873221537	23.87432042901313
14	22.543934591775137	28.126727677681895	26.73596816605679	22.59336956448618
15	22.51879784379193	27.877757332127885	26.632995615328735	22.970449208751454
16	23.102751123739598	26.594954136567868	26.52111197847999	23.78118276121255
17	24.674986964990186	26.30443188438726	26.555581442921344	22.464999707701217
18	22.795321021285318	26.639372182005218	26.41115647137665	24.15415032533281
19	23.230429317274364	27.768592974084704	26.368803940850853	22.632173767790075
20	24.208995431137865	26.614112261114435	26.531671686594304	22.645220621153392
21	23.010025611437417	27.698730248395222	26.530496806552872	22.760747333614482
22	24.270060768775174	26.389383291253992	26.662064421515126	22.678491518455708
23	22.756597354113296	26.677105728497157	27.900766978745757	22.665529938643786
24	22.73110056224642	27.63601155134477	26.60061535289654	23.03227253351227
25	22.98679330610201	26.43829714781763	26.621407887178165	23.953501658902194
26	22.94487661817223	26.984412658043865	27.464659087237592	22.60605163654631
27	23.233844996749657	26.24658799202484	27.885071907869698	22.63449510335581
28	23.735627735089942	26.37761080374207	27.105581637155545	22.781179824012447
29	23.688092467607184	26.414846921184214	27.19944413014298	22.697616481065623
30	23.59146805903848	26.3172892409697	27.156456785401247	22.93478591459058
31	23.44218249506432	26.576411876559142	27.038585050276815	22.942820578099724
32	23.24874891856556	26.675149174234612	27.220231927005084	22.855869980194743
33	23.31811421520524	26.451647196030347	27.211699834446136	23.01853875431828
34	23.447161522981837	26.47108956574823	27.155589837628735	22.926159073641198
35	23.208158708101905	26.634596863127136	27.210108061486775	22.947136367284177
36	23.171955348115535	26.537825594553254	27.16091469717135	23.129304360159857
37	23.533974735720683	26.50044261710593	27.043147185276407	22.92243546189698
38	23.327968047810796	26.653177022492038	27.129875124463855	22.888979805233316
39	23.260871975122104	26.558746039161974	27.12443656685271	23.05594541886321
40	23.320994566274557	26.62620215573433	27.001078899921914	23.051724378069196
41	23.228742795924568	26.658326597512342	27.16576107734226	22.94716952922083
42	23.180823797460533	26.578823223095792	27.161667946875333	23.078685032568337
43	23.292825869797333	26.65762072200358	26.983829955442673	23.065723452756416
44	23.40867472678591	26.535011567357248	27.17227029176535	22.884043414091494
45	23.26020873638904	26.46655585526577	27.1344704214001	23.13876498694509
46	23.42087831947433	26.58373118972048	26.958792693269423	23.036597797535766
47	23.289002772243155	26.631209608168977	27.083012570553205	22.99677504903466
48	21.021413230987385	35.32476289925906	23.97167440018821	19.68214946956535
49	23.39451931725447	26.66858311077726	26.8591789729824	23.077718598985868
50	23.324827138667775	26.67974920859038	27.020810252230635	22.974613400511206
51	23.37504378559993	26.546315050336506	26.95168656398657	23.126954600076992
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16666.0
1	10590.5
2	4515.0
3	3988.5
4	3462.0
5	3697.0
6	3932.0
7	4682.5
8	5433.0
9	6617.5
10	7802.0
11	10830.0
12	13858.0
13	18498.0
14	23138.0
15	30290.0
16	37442.0
17	51163.5
18	64885.0
19	84669.5
20	104454.0
21	135169.5
22	165885.0
23	265448.0
24	365011.0
25	346739.0
26	367770.0
27	407073.0
28	449493.5
29	491914.0
30	538581.5
31	585249.0
32	637744.5
33	690240.0
34	750388.5
35	810537.0
36	877535.0
37	944533.0
38	1000328.5
39	1056124.0
40	1116891.0
41	1177658.0
42	1232141.5
43	1286625.0
44	1335788.5
45	1384952.0
46	1417737.5
47	1450523.0
48	1462502.5
49	1474482.0
50	1469928.5
51	1465375.0
52	1436994.0
53	1408613.0
54	1343451.0
55	1278289.0
56	1190669.0
57	1103049.0
58	1006301.5
59	909554.0
60	804196.5
61	698839.0
62	601953.5
63	505068.0
64	427053.0
65	349038.0
66	281980.5
67	214923.0
68	173785.0
69	132647.0
70	101633.0
71	70619.0
72	52312.5
73	34006.0
74	25399.5
75	12681.0
76	8569.0
77	6364.0
78	4159.0
79	3107.5
80	2056.0
81	1559.0
82	1062.0
83	769.5
84	477.0
85	361.5
86	246.0
87	188.5
88	131.0
89	103.0
90	75.0
91	65.0
92	55.0
93	37.5
94	20.0
95	15.0
96	10.0
97	7.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.1108538E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.32770233268237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.72392551254316	33.22130382510521
2	19.092088607730567	19.599060819335815
3	7.542370100513963	11.61397582206313
4	3.3259166435970697	6.828466378634578
5	1.6510562101250559	4.237246084391277
6	0.9496624755888707	2.924639571812628
7	0.6080130357040114	2.184553847770425
8	0.3827771615636937	1.5717657766792268
9	0.26916566961348487	1.243408983128827
>10	1.376227944257587	12.256999921349454
>50	0.061631772297603846	2.0970540460359666
>100	0.016832211066810706	1.4599013511883
>500	2.1956697272112547E-4	0.06834593716591639
>1k	7.54196665718705E-5	0.0792668181183264
>5k	9.41718971038242E-6	0.025951743914122916
>10k+	2.8251569131074155E-5	0.5880590733067655
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	93183	0.44144696330934907	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1740710796740164E-4	9.474839043803034E-6	0.0	0.0	1.894967808760607E-5
2	3.1740710796740164E-4	9.474839043803034E-6	0.0	0.0	2.3687097609507583E-5
3	3.1740710796740164E-4	9.474839043803034E-6	0.0	0.0	3.789935617521214E-5
4	3.1740710796740164E-4	9.474839043803034E-6	0.0	4.737419521901517E-6	3.789935617521214E-5
5	3.1740710796740164E-4	9.474839043803034E-6	0.0	9.474839043803034E-6	4.263677569711365E-5
6	3.2214452748930317E-4	9.474839043803034E-6	0.0	9.474839043803034E-6	5.211161474091668E-5
7	3.2214452748930317E-4	9.474839043803034E-6	0.0	9.474839043803034E-6	5.68490342628182E-5
8	3.2214452748930317E-4	1.421225856570455E-5	0.0	1.894967808760607E-5	5.68490342628182E-5
9	3.2214452748930317E-4	1.421225856570455E-5	0.0	1.894967808760607E-5	6.158645378471972E-5
10	3.2214452748930317E-4	1.421225856570455E-5	0.0	1.894967808760607E-5	7.106129282852276E-5
11	3.2214452748930317E-4	1.421225856570455E-5	0.0	1.894967808760607E-5	8.053613187232579E-5
12	3.2688194701120465E-4	1.421225856570455E-5	0.0	2.3687097609507583E-5	9.001097091612881E-5
13	3.410942055769092E-4	1.894967808760607E-5	0.0	2.3687097609507583E-5	9.948580995993185E-5
14	3.458316250988107E-4	1.894967808760607E-5	0.0	2.3687097609507583E-5	1.0896064900373488E-4
15	3.5056904462071225E-4	1.894967808760607E-5	0.0	2.3687097609507583E-5	1.4212258565704552E-4
16	3.553064641426138E-4	1.894967808760607E-5	0.0	2.3687097609507583E-5	1.4686000517894702E-4
17	3.647813031864168E-4	1.894967808760607E-5	0.0	2.3687097609507583E-5	1.658096832665531E-4
18	3.789935617521213E-4	1.894967808760607E-5	0.0	2.3687097609507583E-5	1.658096832665531E-4
19	4.12155498405432E-4	1.894967808760607E-5	0.0	2.84245171314091E-5	1.8002194183225763E-4
20	4.21630337449235E-4	1.894967808760607E-5	0.0	3.316193665331062E-5	1.8002194183225763E-4
21	4.3110517649303805E-4	1.894967808760607E-5	0.0	3.316193665331062E-5	1.8949678087606066E-4
22	4.547922741025456E-4	1.894967808760607E-5	0.0	3.316193665331062E-5	2.1318387848556826E-4
23	4.6426711314634866E-4	2.3687097609507583E-5	0.0	3.789935617521214E-5	2.1792129800746976E-4
24	4.8321679123395473E-4	2.84245171314091E-5	0.0	3.789935617521214E-5	2.226587175293713E-4
25	5.069038888434623E-4	2.84245171314091E-5	0.0	3.789935617521214E-5	2.3687097609507583E-4
26	5.542780840624774E-4	2.84245171314091E-5	0.0	4.263677569711365E-5	2.4160839561697736E-4
27	5.969148597595911E-4	2.84245171314091E-5	0.0	4.263677569711365E-5	2.558206541826819E-4
28	6.300767964129017E-4	2.84245171314091E-5	0.0	4.263677569711365E-5	2.6055807370458343E-4
29	6.632387330662124E-4	2.84245171314091E-5	0.0	4.263677569711365E-5	2.7003291274838644E-4
30	7.011380892414245E-4	2.84245171314091E-5	0.0	4.263677569711365E-5	2.795077517921895E-4
31	7.437748649385381E-4	2.84245171314091E-5	0.0	4.263677569711365E-5	2.8424517131409103E-4
32	8.24310996810864E-4	2.84245171314091E-5	0.0	4.263677569711365E-5	2.9845742987979557E-4
33	9.143219677269928E-4	2.84245171314091E-5	0.0	4.7374195219015165E-5	3.079322689235986E-4
34	9.616961629460079E-4	2.84245171314091E-5	0.0	5.211161474091668E-5	3.316193665331062E-4
35	0.001028020036252629	2.84245171314091E-5	0.0	5.211161474091668E-5	3.458316250988107E-4
36	0.0010990813290811519	2.84245171314091E-5	0.0	5.211161474091668E-5	3.647813031864168E-4
37	0.0011511929438220686	3.316193665331062E-5	0.0	8.053613187232579E-5	3.7425614223021985E-4
38	0.0012222542366505914	3.316193665331062E-5	0.0	8.053613187232579E-5	3.884684007959244E-4
39	0.0012696284318696066	3.316193665331062E-5	0.0	1.3738516613514399E-4	3.979432398397274E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	36655	0.0	33.552036	1
TCAACGC	39635	0.0	30.893148	12
CAACGCA	39610	0.0	30.833122	13
AACGCAG	40455	0.0	30.177975	14
ATCAACG	40680	0.0	30.11615	11
ACGCAGA	40570	0.0	30.114616	15
GGTATCA	41185	0.0	29.834286	8
GTGGTAT	41430	0.0	29.54381	6
GTATCAA	41680	0.0	29.528551	9
AGTGGTA	41570	0.0	29.40101	5
CGCAGAG	41630	0.0	29.272158	16
TGGTATC	42200	0.0	29.042063	7
TATCAAC	42550	0.0	28.771444	10
AGAGTAC	42670	0.0	28.347786	19
AGCAGTG	43675	0.0	28.179737	2
GCAGTGG	44155	0.0	27.802061	3
GAGTACT	21625	0.0	27.332947	20
CAGAGTA	44880	0.0	27.227606	18
CAGTGGT	45610	0.0	26.91515	4
GCAGAGT	45955	0.0	26.629854	17
>>END_MODULE