FastQCFastQC Report
Thu 26 May 2016
SRR805447_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805447_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17852717
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3834552.1478803478484534No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTATTT608490.3408388762337968No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC360100.20170599242681098No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT353720.19813230669594997No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG271500.15207769215184447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1919600.043.0519371
TCAACGC1926500.042.3184512
CAACGCA1926150.042.28175413
AGTGGTA1944200.042.1623275
ACGCAGA1930600.042.12252815
GTATCAA1954850.042.100679
ATCAACG1935750.042.0953111
AACGCAG1932500.042.0950814
GTGGTAT1952550.042.0119866
GGTATCA1956100.041.9921048
TGGTATC1956700.041.9401327
CGCAGAG1934350.041.90244716
TATCAAC1957100.041.78899810
AGAGTAC1926200.041.67207319
AGCAGTG1983200.041.624772
GCAGTGG1982400.041.5609863
CAGTGGT1986950.041.3333244
CAGAGTA1968700.041.26733418
GCAGAGT1970550.041.16464617
GTACATG1324350.040.87665622