##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805447_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17852717
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.95934960488087	37.0	37.0	37.0	35.0	37.0
2	36.038065746519145	37.0	37.0	37.0	35.0	37.0
3	37.840808376674545	39.0	39.0	39.0	35.0	39.0
4	37.82512112862149	39.0	38.0	39.0	35.0	39.0
5	37.85603911158173	39.0	38.0	39.0	35.0	39.0
6	37.88075238071605	39.0	38.0	39.0	35.0	39.0
7	37.76991110092654	39.0	38.0	39.0	35.0	39.0
8	38.682091302965254	40.0	39.0	40.0	36.0	40.0
9	38.70109238834627	40.0	39.0	40.0	36.0	40.0
10	38.44161698188573	40.0	38.0	40.0	36.0	40.0
11	38.66825604192348	40.0	39.0	40.0	36.0	40.0
12	38.75432803869573	40.0	39.0	40.0	36.0	40.0
13	38.835112268905625	40.0	39.0	40.0	36.0	40.0
14	38.79537025092595	40.0	39.0	40.0	36.0	40.0
15	38.77437708781246	40.0	39.0	40.0	36.0	40.0
16	38.50779940106595	40.0	39.0	40.0	36.0	40.0
17	38.273720241014296	40.0	39.0	40.0	35.0	40.0
18	38.49284369432395	40.0	39.0	40.0	35.0	40.0
19	38.505380161462256	40.0	39.0	40.0	35.0	40.0
20	38.39955878984695	40.0	39.0	40.0	35.0	40.0
21	38.30963875134524	40.0	38.0	40.0	35.0	40.0
22	38.17103469460699	40.0	38.0	40.0	34.0	40.0
23	37.85456443408586	40.0	38.0	40.0	34.0	40.0
24	38.05016317684306	40.0	38.0	40.0	34.0	40.0
25	37.91523486313036	40.0	38.0	40.0	34.0	40.0
26	37.929091129378236	40.0	38.0	40.0	34.0	40.0
27	37.65973005677511	40.0	38.0	40.0	33.0	40.0
28	37.79352308110861	40.0	38.0	40.0	33.0	40.0
29	37.836319648152156	40.0	38.0	40.0	34.0	40.0
30	37.751270072785	40.0	38.0	40.0	33.0	40.0
31	37.61508267901183	40.0	38.0	40.0	33.0	40.0
32	37.50075868003733	40.0	38.0	40.0	33.0	40.0
33	37.353130002564875	40.0	37.0	40.0	32.0	40.0
34	37.065219820602096	40.0	37.0	40.0	31.0	40.0
35	37.07925639553912	40.0	37.0	40.0	31.0	40.0
36	36.973857984753806	39.0	37.0	40.0	31.0	40.0
37	36.46103307412535	39.0	36.0	40.0	30.0	40.0
38	36.49990861335	39.0	35.0	40.0	30.0	40.0
39	36.46508836722164	39.0	35.0	40.0	30.0	40.0
40	36.335545116185955	39.0	35.0	40.0	30.0	40.0
41	36.49041140348553	39.0	35.0	40.0	30.0	40.0
42	36.44491043016029	39.0	35.0	40.0	30.0	40.0
43	36.381059364801445	39.0	35.0	40.0	30.0	40.0
44	36.24481198015966	39.0	35.0	40.0	30.0	40.0
45	36.11970665305454	39.0	35.0	40.0	29.0	40.0
46	35.880924455364415	39.0	35.0	40.0	28.0	40.0
47	35.68368585017059	38.0	35.0	40.0	28.0	40.0
48	30.170983890015172	37.0	27.0	40.0	3.0	40.0
49	31.485361191800667	37.0	26.0	40.0	12.0	40.0
50	33.184911966060966	36.0	29.0	40.0	20.0	40.0
51	33.696394391957256	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	54.0
9	169.0
10	225.0
11	350.0
12	540.0
13	869.0
14	1119.0
15	1569.0
16	2310.0
17	3741.0
18	6192.0
19	10412.0
20	16811.0
21	25545.0
22	36658.0
23	49691.0
24	66478.0
25	85843.0
26	107455.0
27	131983.0
28	161201.0
29	194927.0
30	235800.0
31	287444.0
32	354739.0
33	456792.0
34	620627.0
35	925230.0
36	1550528.0
37	2822935.0
38	3793685.0
39	5900790.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.651484141041388	35.73307077012423	27.092296371471075	22.523148717363302
2	25.36641901622033	37.691142474279964	18.23969427174586	18.702744237753837
3	33.59518890037858	24.051857204704472	20.534734292825007	21.81821960209194
4	21.445760888944804	23.240272054948274	21.659168181515454	33.65479887459147
5	21.875902698732077	33.72697836413359	23.127807380803716	21.269311556330614
6	32.51567254440879	23.17602973261717	22.167886266275325	22.14041145669872
7	22.513805601690766	22.867314818243074	33.35646333272409	21.262416247342074
8	31.747033238694144	23.836164545710325	23.235214001319797	21.18158821427573
9	31.570175004734573	23.395794600900246	23.741809159916667	21.292221234448515
10	21.34937780058912	23.784452528990403	33.48266821235109	21.38350145806938
11	21.13197111677735	34.00589949417783	23.453600928082825	21.408528460961993
12	21.056918115040975	23.727127921201014	33.59829206949284	21.61766189426517
13	21.13739886203316	23.83060236713549	23.395060819033876	31.636937951797478
14	21.450124370424962	34.1011174937686	23.150722660309913	21.29803547549653
15	21.280553542634433	33.80084947294017	23.164944585185548	21.753652399239847
16	21.771929729239535	23.35729065777495	23.402953175138553	31.46782643784697
17	32.31254379935558	23.07472862534033	23.35735227304617	21.255375302257914
18	21.487832916412668	23.52956695611094	23.218297808675285	31.764302318801107
19	21.764255827278276	33.64129952880561	23.200474191127324	21.393970452788782
20	31.97714387115418	23.537168040024383	23.235230805484676	21.25045728333676
21	21.69736404828464	33.6426494633842	23.205745097510928	21.45424139082023
22	31.827962096749758	23.204064681023063	23.602345794200403	21.36562742802678
23	21.628719034755324	23.55853173497345	33.42535480733829	21.387394422932935
24	21.544233295133733	33.403918294341416	23.280680470093152	21.7711679404317
25	21.790296681451903	23.37723160009762	23.40029251569943	31.432179202751044
26	21.82117153372229	30.28047215446254	26.521296450282613	21.37705986153256
27	22.011081002404286	23.3442618286057	33.27576413158849	21.368893037401534
28	28.841419488137294	23.257356289241578	26.42829660045583	21.472927622165297
29	28.970128188331223	23.378211843048877	26.254205452312945	21.397454516306958
30	28.81025336367568	23.27472059294952	26.2813273744271	21.6336986689477
31	26.102127760161096	24.251025768234605	26.998596348107682	22.64825012349661
32	24.712227276105928	25.47488429912377	27.3618631830662	22.451025241704105
33	24.33328775670392	25.017788608871133	27.635989524731723	23.01293410969322
34	23.951855619511583	24.598569506254986	28.116314172234958	23.33326070199847
35	23.448772531374356	25.07197643921651	28.08999324864669	23.389257780762446
36	23.55594949497043	25.07521964303809	28.106971056562426	23.261859805429054
37	24.422669109693498	24.643683087565886	28.106478134392653	22.82716966834796
38	24.113999006425747	24.894378822002274	28.030657742460154	22.960964429111826
39	24.146240597439594	24.862366887908436	27.913011784144675	23.078380730507295
40	23.6415555122506	25.204012364056403	27.704242441080535	23.450189682612457
41	23.880695582638765	25.50773084007325	27.59791688850498	23.013656688783
42	23.822788430466915	25.463054167049194	27.754514901009188	22.959642501474704
43	23.756708852775745	25.050153430427425	28.319538140889144	22.87359957590769
44	23.970989961920083	24.824333461399743	27.91113531906656	23.29354125761362
45	24.00921383563073	24.599241672850134	27.85050029079607	23.54104420072306
46	23.933707121442634	24.65419129200334	27.714397758055537	23.697703828498486
47	23.764377153348704	24.81727011076241	27.781356753708693	23.636995982180192
48	21.25945871432343	34.769682396242544	24.387755656463945	19.583103232970085
49	23.47961937670328	25.366256575959838	27.62997923509346	23.524144812243424
50	24.02954687513391	25.11241846269114	27.63551340672683	23.222521255448118
51	23.946691139505543	24.845047395306832	27.709597368288534	23.498664096899088
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5934.0
1	3644.0
2	1354.0
3	1158.5
4	963.0
5	1086.0
6	1209.0
7	1467.0
8	1725.0
9	2173.0
10	2621.0
11	3666.5
12	4712.0
13	6494.5
14	8277.0
15	11101.5
16	13926.0
17	19975.5
18	26025.0
19	35584.0
20	45143.0
21	66874.0
22	88605.0
23	342759.0
24	596913.0
25	409473.0
26	228696.5
27	235360.0
28	261613.0
29	287866.0
30	316718.0
31	345570.0
32	382474.5
33	419379.0
34	465771.5
35	512164.0
36	570883.5
37	629603.0
38	684713.5
39	739824.0
40	803607.0
41	867390.0
42	926806.0
43	986222.0
44	1056431.5
45	1126641.0
46	1193467.5
47	1260294.0
48	1286783.0
49	1313272.0
50	1335802.0
51	1358332.0
52	1360966.5
53	1363601.0
54	1334115.5
55	1304630.0
56	1225102.5
57	1145575.0
58	1028704.0
59	911833.0
60	803441.0
61	695049.0
62	601562.5
63	508076.0
64	431026.5
65	353977.0
66	282536.5
67	211096.0
68	171219.5
69	131343.0
70	98661.0
71	65979.0
72	48134.0
73	30289.0
74	22443.0
75	10957.0
76	7317.0
77	5497.0
78	3677.0
79	2805.0
80	1933.0
81	1502.5
82	1072.0
83	819.0
84	566.0
85	431.0
86	296.0
87	245.0
88	194.0
89	168.0
90	142.0
91	105.0
92	68.0
93	46.5
94	25.0
95	21.0
96	17.0
97	12.5
98	8.0
99	4.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.7852717E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.540661692904806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.4877903314961	37.6466221139617
2	13.877448277965978	14.02750837154926
3	4.509338074614777	6.837147902641207
4	2.0138414566485072	4.071235190544753
5	1.1482119949198317	2.9015696993489275
6	0.7494117927720081	2.272546073229801
7	0.5256933334757089	1.8598222244987836
8	0.4048994633720834	1.6371109438341735
9	0.3073088444575365	1.3978433108669273
>10	1.8327284126315415	15.901981975052577
>50	0.10047386988655206	3.483796686003297
>100	0.04045985175540517	3.574056329363288
>500	0.001791390170204678	0.5823136018564666
>1k	4.7782102016704123E-4	0.45658476601888504
>5k	6.822808012140694E-5	0.23009879905094327
>10k+	5.685673343450559E-5	3.119762012179172
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	383455	2.1478803478484534	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTATTT	60849	0.3408388762337968	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC	36010	0.20170599242681098	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT	35372	0.19813230669594997	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG	27150	0.15207769215184447	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.161527122174176E-5	0.0	0.0	5.601388292885615E-6	1.6804164878656847E-5
2	1.1762915415059792E-4	0.0	0.0	5.601388292885615E-6	1.6804164878656847E-5
3	1.1762915415059792E-4	0.0	0.0	1.120277658577123E-5	2.240555317154246E-5
4	1.1762915415059792E-4	0.0	0.0	1.120277658577123E-5	2.240555317154246E-5
5	1.1762915415059792E-4	0.0	0.0	1.6804164878656847E-5	2.240555317154246E-5
6	1.2883193073636915E-4	0.0	0.0	1.6804164878656847E-5	2.8006941464428077E-5
7	1.2883193073636915E-4	0.0	0.0	2.240555317154246E-5	2.8006941464428077E-5
8	1.2883193073636915E-4	0.0	0.0	2.240555317154246E-5	3.920971805019931E-5
9	1.3443331902925477E-4	0.0	0.0	2.240555317154246E-5	5.0412494635970534E-5
10	1.45636095615026E-4	0.0	0.0	2.240555317154246E-5	5.6013882928856154E-5
11	1.45636095615026E-4	0.0	0.0	2.240555317154246E-5	5.6013882928856154E-5
12	1.5683887220079723E-4	0.0	0.0	2.240555317154246E-5	5.6013882928856154E-5
13	1.6804164878656845E-4	0.0	0.0	2.240555317154246E-5	6.161527122174176E-5
14	1.7924442537233968E-4	0.0	0.0	2.240555317154246E-5	7.2818047807513E-5
15	2.0725136683676776E-4	0.0	0.0	2.240555317154246E-5	7.2818047807513E-5
16	2.0725136683676776E-4	0.0	0.0	3.920971805019931E-5	7.841943610039861E-5
17	2.2405553171542461E-4	0.0	0.0	3.920971805019931E-5	8.962221268616984E-5
18	2.632652497656239E-4	0.0	0.0	4.481110634308492E-5	1.0082498927194107E-4
19	2.744680263513951E-4	0.0	0.0	4.481110634308492E-5	1.0082498927194107E-4
20	3.024749678158232E-4	0.0	0.0	4.481110634308492E-5	1.1202776585771231E-4
21	3.5848885074467936E-4	0.0	0.0	4.481110634308492E-5	1.1202776585771231E-4
22	3.9209718050199307E-4	0.0	0.0	4.481110634308492E-5	1.1202776585771231E-4
23	4.089013453806499E-4	0.0	0.0	4.481110634308492E-5	1.1202776585771231E-4
24	4.145027336735355E-4	0.0	0.0	4.481110634308492E-5	1.1762915415059792E-4
25	4.6491522830950606E-4	0.0	0.0	4.481110634308492E-5	1.1762915415059792E-4
26	5.433346644099046E-4	0.0	0.0	4.481110634308492E-5	1.1762915415059792E-4
27	5.82544382460104E-4	0.0	0.0	4.481110634308492E-5	1.2323054244348352E-4
28	6.665652068533882E-4	0.0	0.0	4.481110634308492E-5	1.3443331902925477E-4
29	7.225790897822444E-4	0.0	0.0	4.481110634308492E-5	1.4003470732214037E-4
30	8.065999141755285E-4	5.601388292885615E-6	0.0	4.481110634308492E-5	1.45636095615026E-4
31	9.634387863763258E-4	5.601388292885615E-6	0.0	4.481110634308492E-5	1.5683887220079723E-4
32	0.0012042984829704073	5.601388292885615E-6	0.0	4.481110634308492E-5	1.6244026049368283E-4
33	0.0013163262488281196	5.601388292885615E-6	0.0	5.6013882928856154E-5	1.6804164878656845E-4
34	0.0014003470732214037	5.601388292885615E-6	0.0	5.6013882928856154E-5	1.7924442537233968E-4
35	0.0014787665093218025	5.601388292885615E-6	0.0	6.161527122174176E-5	1.7924442537233968E-4
36	0.0015795914985937435	5.601388292885615E-6	0.0	6.161527122174176E-5	1.848458136652253E-4
37	0.0017196262059158839	5.601388292885615E-6	0.0	1.0082498927194107E-4	1.848458136652253E-4
38	0.0019100734078739948	5.601388292885615E-6	0.0	1.45636095615026E-4	1.904472019581109E-4
39	0.002078115056660563	5.601388292885615E-6	0.0	2.632652497656239E-4	1.9604859025099653E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	191960	0.0	43.051937	1
TCAACGC	192650	0.0	42.31845	12
CAACGCA	192615	0.0	42.281754	13
AGTGGTA	194420	0.0	42.162327	5
ACGCAGA	193060	0.0	42.122528	15
GTATCAA	195485	0.0	42.10067	9
ATCAACG	193575	0.0	42.09531	11
AACGCAG	193250	0.0	42.09508	14
GTGGTAT	195255	0.0	42.011986	6
GGTATCA	195610	0.0	41.992104	8
TGGTATC	195670	0.0	41.940132	7
CGCAGAG	193435	0.0	41.902447	16
TATCAAC	195710	0.0	41.788998	10
AGAGTAC	192620	0.0	41.672073	19
AGCAGTG	198320	0.0	41.62477	2
GCAGTGG	198240	0.0	41.560986	3
CAGTGGT	198695	0.0	41.333324	4
CAGAGTA	196870	0.0	41.267334	18
GCAGAGT	197055	0.0	41.164646	17
GTACATG	132435	0.0	40.876656	22
>>END_MODULE