FastQCFastQC Report
Thu 26 May 2016
SRR805446_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805446_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23317805
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6610022.8347522419027005No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC753600.3231865091932967No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT407170.1746176366085916No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG276950.11877189984220213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2532700.043.2596661
TCAACGC2605400.042.20973612
CAACGCA2615100.042.15125313
GTGGTAT2602800.042.1127246
GTATCAA2610900.042.0863539
GGTATCA2613200.042.0432788
AGTGGTA2611300.042.0428545
ACGCAGA2621500.042.02689415
AACGCAG2623350.042.00840414
ATCAACG2618000.041.98596211
TGGTATC2620950.041.924117
CGCAGAG2631900.041.86680616
TATCAAC2626950.041.82407410
AGAGTAC2620200.041.63384619
AGCAGTG2655150.041.4247782
CAGTGGT2653950.041.393494
GCAGTGG2659600.041.33773
CAGAGTA2647700.041.32378818
GCAGAGT2674250.041.1078817
GTACATG1685900.040.9401522