##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805446_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23317805
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.75732102571404	35.0	35.0	35.0	33.0	37.0
2	35.68975819121911	36.0	35.0	37.0	35.0	37.0
3	38.07526415972687	39.0	39.0	39.0	35.0	39.0
4	38.08843696908864	39.0	39.0	39.0	37.0	39.0
5	38.09479850268925	39.0	39.0	39.0	37.0	39.0
6	37.76757940981152	39.0	38.0	39.0	35.0	39.0
7	37.741495565298706	39.0	37.0	39.0	35.0	39.0
8	38.831869809358125	40.0	39.0	40.0	36.0	40.0
9	38.81005532038714	40.0	39.0	40.0	36.0	40.0
10	38.65995937439223	40.0	38.0	40.0	36.0	40.0
11	38.572151881362764	40.0	38.0	40.0	36.0	40.0
12	38.530593767294995	40.0	38.0	40.0	36.0	40.0
13	38.602403828319176	40.0	38.0	40.0	36.0	40.0
14	38.492500001608214	40.0	38.0	40.0	36.0	40.0
15	38.43997867723827	40.0	38.0	40.0	36.0	40.0
16	38.325166669847356	40.0	38.0	40.0	35.0	40.0
17	38.02815608072887	40.0	38.0	40.0	34.0	40.0
18	38.028458339024624	40.0	38.0	40.0	34.0	40.0
19	37.92524141959331	39.0	38.0	40.0	34.0	40.0
20	37.62170684590595	39.0	37.0	40.0	34.0	40.0
21	37.224949217990286	38.0	36.0	40.0	33.0	40.0
22	37.36825644609345	39.0	37.0	40.0	33.0	40.0
23	37.04936699659338	38.0	36.0	40.0	33.0	40.0
24	36.850017186437576	38.0	36.0	40.0	32.0	40.0
25	36.54069866353201	38.0	36.0	40.0	32.0	40.0
26	36.52087321255152	38.0	36.0	40.0	31.0	40.0
27	36.30088402403228	38.0	35.0	39.0	31.0	40.0
28	36.023884323588774	38.0	35.0	39.0	31.0	40.0
29	35.52135970774265	37.0	34.0	39.0	30.0	40.0
30	35.35518561888651	37.0	34.0	39.0	30.0	40.0
31	35.08423447232705	37.0	34.0	38.0	29.0	40.0
32	34.838386460475164	37.0	33.0	38.0	29.0	40.0
33	34.52137021473505	36.0	33.0	38.0	28.0	40.0
34	34.21774249334361	36.0	32.0	38.0	27.0	39.0
35	33.9195703454935	36.0	32.0	38.0	27.0	39.0
36	33.79498340431271	35.0	32.0	38.0	27.0	39.0
37	33.56859893116012	35.0	31.0	38.0	27.0	39.0
38	33.31554556700341	35.0	31.0	38.0	26.0	39.0
39	33.0458918410202	35.0	31.0	38.0	26.0	39.0
40	32.79418843240176	34.0	31.0	37.0	25.0	38.0
41	32.46456606871873	34.0	30.0	37.0	24.0	38.0
42	32.146287482891296	34.0	30.0	37.0	24.0	38.0
43	31.832698103444987	33.0	29.0	37.0	24.0	38.0
44	31.430783171915195	33.0	29.0	36.0	23.0	38.0
45	30.934691665875068	33.0	28.0	36.0	22.0	38.0
46	30.682065657552243	33.0	28.0	36.0	22.0	38.0
47	30.29618280965983	32.0	27.0	35.0	21.0	38.0
48	29.861658247849657	31.0	27.0	35.0	20.0	37.0
49	29.398256954288794	31.0	26.0	35.0	19.0	37.0
50	29.14563643533343	31.0	26.0	34.0	18.0	37.0
51	30.59894964384512	32.0	28.0	35.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	69.0
8	318.0
9	635.0
10	754.0
11	776.0
12	765.0
13	846.0
14	912.0
15	1214.0
16	1794.0
17	2968.0
18	5667.0
19	10869.0
20	19594.0
21	32908.0
22	51104.0
23	73576.0
24	100605.0
25	133201.0
26	172616.0
27	215663.0
28	268029.0
29	330625.0
30	412604.0
31	530767.0
32	722087.0
33	1090324.0
34	1951291.0
35	4253613.0
36	7395206.0
37	4947064.0
38	586435.0
39	2903.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.29719070898826	45.561668433199436	20.07147756832172	21.069663289490585
2	26.581206936073098	42.07960826501465	17.04943497040137	14.289749828510873
3	35.116671573503595	23.664204242208907	21.031657996968413	20.18746618731909
4	20.859360475825234	24.60970490146907	20.95303567381235	33.57789894889335
5	19.822110185757193	35.428519108037825	24.028196479042517	20.72117422716246
6	31.746954741237438	22.695601065366144	23.22452306295554	22.33292113044088
7	22.82005531824286	23.231461966510143	32.97802258831824	20.970460126928756
8	30.84057011369638	23.83087516170583	24.096714935217957	21.231839789379833
9	31.526316477901755	23.103765555977503	24.265543004583837	21.104374961536905
10	21.351422228636014	24.25097902654216	33.37928248392162	21.0183162609002
11	20.986812437963177	34.390295312959346	23.550467121583697	21.072425127493776
12	21.203590989803715	23.587267326405723	33.70344678669369	21.505694897096873
13	21.205893950995815	24.295558694311065	23.072836401196426	31.425710953496694
14	21.343398317294447	34.70446725152732	22.988188639539615	20.963945791638622
15	21.26557795641571	34.15582212819774	22.902022724694714	21.676577190691834
16	21.703483668381306	23.71399023192792	22.934838849540085	31.647687250150693
17	31.961194460627834	23.462049708366635	23.13230597819992	21.444449852805615
18	21.40633734607524	23.561677439192927	22.99698449318021	32.03500072155162
19	21.77083563397155	34.1245455993821	22.852386834867175	21.25223193177917
20	32.05024229338911	23.92221737852255	22.99393103253072	21.033609295557625
21	21.888123689172286	33.84638476906382	22.90448436291495	21.361007178848954
22	31.780032468750814	23.654494923514456	23.308167299623612	21.257305308111118
23	21.46892471225315	23.76881100086393	33.63526712741615	21.126997159466768
24	21.527497978476102	33.59816672281117	23.072038727487428	21.802296571225295
25	21.37532670849593	23.811520852841852	23.052246984654	31.760905454008213
26	21.527858218215652	30.147884845936403	27.138849475754682	21.185407460093263
27	21.888852745788036	23.26318021786356	33.398499558599106	21.449467477749298
28	28.384777212091787	23.457280820385968	26.769247791548135	21.38869417597411
29	28.509741804599532	23.48142117150392	26.844473568588466	21.16436345530808
30	28.34077650104716	23.19141102689554	26.826024147641686	21.64178832441561
31	25.705700000493188	24.364330176017855	27.405199588897837	22.52477023459112
32	24.17354034824461	25.750382593901953	27.84630028426775	22.229776773585677
33	23.92184427307802	25.107474738724335	27.9591539598174	23.011527028380243
34	23.39764398921768	24.9352758546527	28.452330740393446	23.214749415736172
35	23.125568637356732	25.369553437812865	28.40803411813419	23.096843806696214
36	23.415407239231996	25.014691562949427	28.395447170091696	23.17445402772688
37	23.641753587012158	24.930717106520103	28.50811214863492	22.91941715783282
38	23.687126639921726	24.93637801671298	28.68054261539626	22.695952727969036
39	24.015498028223497	24.738949485168092	28.297817912106222	22.94773457450219
40	23.302424906632506	25.243186483461887	28.07863347343371	23.37575513647189
41	23.459901135634336	25.594343035289985	28.194827943710827	22.750927885364852
42	23.59216487143623	25.30087201604096	28.17924757497543	22.927715537547382
43	23.557487507936532	25.11455087646543	28.649935103239777	22.678026512358258
44	23.527621918100785	24.934503912353673	28.36626346262009	23.171610706925456
45	23.703041516986698	24.521115945518883	28.256664810431342	23.519177727063077
46	23.431077667902276	24.857412608090684	28.0772654201371	23.634244303869938
47	23.418018977343706	24.909973301517873	28.21892112057717	23.453086600561246
48	23.728511324286313	24.572368625606057	28.21177636574283	23.487343684364802
49	23.3210287160391	25.448561732118442	27.963026537017527	23.267383014824937
50	23.605721035920833	25.128287160819813	28.28816005623171	22.977831747027647
51	23.49493016173692	24.901391876293673	28.093917930954476	23.509760031014928
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18356.0
1	11235.0
2	4114.0
3	3256.5
4	2399.0
5	2328.5
6	2258.0
7	2473.0
8	2688.0
9	3286.5
10	3885.0
11	5524.0
12	7163.0
13	9892.0
14	12621.0
15	16966.5
16	21312.0
17	30318.0
18	39324.0
19	52929.0
20	66534.0
21	91057.0
22	115580.0
23	502469.0
24	889358.0
25	615449.0
26	340496.0
27	339452.0
28	367332.0
29	395212.0
30	428507.5
31	461803.0
32	505008.0
33	548213.0
34	605769.0
35	663325.0
36	737187.5
37	811050.0
38	883260.0
39	955470.0
40	1037696.5
41	1119923.0
42	1199952.0
43	1279981.0
44	1371341.0
45	1462701.0
46	1573149.5
47	1683598.0
48	1696058.5
49	1708519.0
50	1741869.0
51	1775219.0
52	1779918.0
53	1784617.0
54	1723558.5
55	1662500.0
56	1549664.5
57	1436829.0
58	1292306.0
59	1147783.0
60	1011862.5
61	875942.0
62	760841.0
63	645740.0
64	552428.0
65	459116.0
66	359969.0
67	260822.0
68	214020.5
69	167219.0
70	121901.0
71	76583.0
72	55622.5
73	34662.0
74	25671.5
75	12357.5
76	8034.0
77	5978.5
78	3923.0
79	3342.5
80	2762.0
81	2231.0
82	1700.0
83	1258.5
84	817.0
85	565.0
86	313.0
87	207.5
88	102.0
89	67.5
90	33.0
91	23.0
92	13.0
93	9.0
94	5.0
95	5.0
96	5.0
97	4.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.3317805E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.83802819176736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.00057804763088	27.770095233656267
2	15.984189100164215	13.055255301900933
3	6.046896619957944	7.408300039156359
4	2.885044837983054	4.712781697122594
5	1.6596769871310109	3.388896779484186
6	1.055609570153038	2.5865408031245507
7	0.6838202844291812	1.9548110437534891
8	0.5109565744943873	1.669316719517653
9	0.38749669724107444	1.4242140941532966
>10	2.5513243083760244	19.04916724520375
>50	0.1587718374856883	4.407567357939685
>100	0.06691206430291977	4.870317975702477
>500	0.00561820950419198	1.572544266123375
>1k	0.002937571850094382	1.9325819611167139
>5k	5.576309870820536E-5	0.15742208343993683
>10k+	1.1152619741638806E-4	4.040187398604789
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	661002	2.8347522419027005	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC	75360	0.3231865091932967	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT	40717	0.1746176366085916	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG	27695	0.11877189984220213	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.146281993523833E-5	8.577136655873056E-6	0.0	4.288568327936528E-6	0.0
2	5.5751388263174855E-5	8.577136655873056E-6	0.0	4.288568327936528E-6	0.0
3	6.003995659111138E-5	8.577136655873056E-6	0.0	8.577136655873056E-6	4.288568327936528E-6
4	8.148279823079402E-5	8.577136655873056E-6	0.0	8.577136655873056E-6	8.577136655873056E-6
5	8.148279823079402E-5	8.577136655873056E-6	0.0	8.577136655873056E-6	1.715427331174611E-5
6	9.43485032146036E-5	1.2865704983809582E-5	0.0	8.577136655873056E-6	2.144284163968264E-5
7	9.43485032146036E-5	1.2865704983809582E-5	0.0	1.715427331174611E-5	2.144284163968264E-5
8	9.43485032146036E-5	1.715427331174611E-5	0.0	2.144284163968264E-5	3.001997829555569E-5
9	9.43485032146036E-5	2.144284163968264E-5	0.0	2.144284163968264E-5	4.288568327936528E-5
10	9.43485032146036E-5	2.144284163968264E-5	4.288568327936528E-6	2.144284163968264E-5	4.71742516073018E-5
11	9.43485032146036E-5	2.144284163968264E-5	4.288568327936528E-6	3.001997829555569E-5	6.432852491904791E-5
12	9.43485032146036E-5	2.5731409967619163E-5	4.288568327936528E-6	3.430854662349222E-5	6.861709324698444E-5
13	9.43485032146036E-5	2.5731409967619163E-5	4.288568327936528E-6	3.430854662349222E-5	7.71942299028575E-5
14	1.0292563987047665E-4	2.5731409967619163E-5	4.288568327936528E-6	3.859711495142875E-5	8.148279823079402E-5
15	1.243684815101593E-4	2.5731409967619163E-5	4.288568327936528E-6	4.288568327936528E-5	8.577136655873056E-5
16	1.372341864939689E-4	2.5731409967619163E-5	4.288568327936528E-6	4.288568327936528E-5	1.0292563987047665E-4
17	1.5009989147777845E-4	2.5731409967619163E-5	4.288568327936528E-6	4.288568327936528E-5	1.1150277652634971E-4
18	1.7583130144539763E-4	2.5731409967619163E-5	4.288568327936528E-6	5.146281993523833E-5	1.2007991318222276E-4
19	1.9298557475714374E-4	2.5731409967619163E-5	4.288568327936528E-6	5.5751388263174855E-5	1.2865704983809582E-4
20	2.0156271141301679E-4	2.5731409967619163E-5	4.288568327936528E-6	6.003995659111138E-5	1.2865704983809582E-4
21	2.315826897085725E-4	2.5731409967619163E-5	4.288568327936528E-6	6.003995659111138E-5	1.415227548219054E-4
22	2.35871258036509E-4	2.5731409967619163E-5	4.288568327936528E-6	6.003995659111138E-5	1.54388459805715E-4
23	2.616026680041282E-4	2.5731409967619163E-5	4.288568327936528E-6	6.432852491904791E-5	1.6296559646158804E-4
24	2.7875694131587427E-4	2.5731409967619163E-5	4.288568327936528E-6	6.432852491904791E-5	1.6725416478952456E-4
25	3.001997829555569E-4	3.001997829555569E-5	4.288568327936528E-6	6.432852491904791E-5	1.6725416478952456E-4
26	3.473740345628587E-4	3.430854662349222E-5	4.288568327936528E-6	6.861709324698444E-5	1.6725416478952456E-4
27	3.988368544980971E-4	3.430854662349222E-5	4.288568327936528E-6	8.148279823079402E-5	1.6725416478952456E-4
28	4.4601110610539884E-4	3.859711495142875E-5	4.288568327936528E-6	8.577136655873056E-5	1.715427331174611E-4
29	5.189167676803198E-4	3.859711495142875E-5	4.288568327936528E-6	9.005993488666708E-5	1.715427331174611E-4
30	5.961109975831774E-4	3.859711495142875E-5	4.288568327936528E-6	9.43485032146036E-5	1.8440843810127067E-4
31	6.861709324698444E-4	3.859711495142875E-5	4.288568327936528E-6	1.0721420819841319E-4	1.9727414308508026E-4
32	8.662908022431786E-4	3.859711495142875E-5	4.288568327936528E-6	1.1579134485428624E-4	2.058512797409533E-4
33	0.0010464106720165128	3.859711495142875E-5	4.288568327936528E-6	1.1579134485428624E-4	2.1013984806888986E-4
34	0.0011922219951663547	3.859711495142875E-5	4.288568327936528E-6	1.2007991318222276E-4	2.1442841639682638E-4
35	0.0012951476350368312	3.859711495142875E-5	4.288568327936528E-6	1.243684815101593E-4	2.4015982636444553E-4
36	0.001406650411563181	3.859711495142875E-5	4.288568327936528E-6	1.243684815101593E-4	2.5302553134825515E-4
37	0.0014666903681542923	4.71742516073018E-5	4.288568327936528E-6	1.3294561816603234E-4	2.5731409967619164E-4
38	0.0015653274396968326	4.71742516073018E-5	4.288568327936528E-6	1.4581132314984193E-4	2.658912363320647E-4
39	0.001715427331174611	5.146281993523833E-5	8.577136655873056E-6	1.5009989147777845E-4	2.658912363320647E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	253270	0.0	43.259666	1
TCAACGC	260540	0.0	42.209736	12
CAACGCA	261510	0.0	42.151253	13
GTGGTAT	260280	0.0	42.112724	6
GTATCAA	261090	0.0	42.086353	9
GGTATCA	261320	0.0	42.043278	8
AGTGGTA	261130	0.0	42.042854	5
ACGCAGA	262150	0.0	42.026894	15
AACGCAG	262335	0.0	42.008404	14
ATCAACG	261800	0.0	41.985962	11
TGGTATC	262095	0.0	41.92411	7
CGCAGAG	263190	0.0	41.866806	16
TATCAAC	262695	0.0	41.824074	10
AGAGTAC	262020	0.0	41.633846	19
AGCAGTG	265515	0.0	41.424778	2
CAGTGGT	265395	0.0	41.39349	4
GCAGTGG	265960	0.0	41.3377	3
CAGAGTA	264770	0.0	41.323788	18
GCAGAGT	267425	0.0	41.10788	17
GTACATG	168590	0.0	40.94015	22
>>END_MODULE