FastQCFastQC Report
Thu 26 May 2016
SRR805445_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805445_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27809532
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2379080.8554908439307788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGTAC1095500.035.98151419
AAGCAGT1147950.035.3724021
TCAACGC1142050.035.2556812
CAACGCA1140800.035.15822213
CAGAGTA1138550.034.95103518
ACGCAGA1152500.034.75639715
ATCAACG1160900.034.67740611
AACGCAG1156550.034.65025314
CGCAGAG1169150.034.1825216
GAGTACT450200.033.89493620
GTGGTAT1192100.033.856646
GGTATCA1201050.033.7485968
TGGTATC1197950.033.7251177
GAGTACA720950.033.70240420
AGTGGTA1199300.033.6702655
GTACATG710750.033.65107322
GTATCAA1218550.033.332249
ACATGGG717400.033.2670124
TACATGG728400.032.8572923
GTACTTT460450.032.75925422
Produced by FastQC (version 0.11.5)