##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805445_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27809532
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.12972677857362	37.0	35.0	37.0	35.0	37.0
2	36.146350179499606	37.0	35.0	37.0	35.0	37.0
3	37.84467494814368	39.0	38.0	39.0	35.0	39.0
4	37.83977576465508	39.0	38.0	39.0	35.0	39.0
5	37.88165101088361	39.0	38.0	39.0	35.0	39.0
6	37.829327620472	39.0	38.0	39.0	35.0	39.0
7	37.75985985668511	39.0	38.0	39.0	35.0	39.0
8	38.71762343933008	40.0	39.0	40.0	36.0	40.0
9	38.69295696885514	40.0	39.0	40.0	36.0	40.0
10	38.62501929194637	40.0	39.0	40.0	36.0	40.0
11	38.730139759273904	40.0	39.0	40.0	36.0	40.0
12	38.71644193796573	40.0	39.0	40.0	36.0	40.0
13	38.79845762956385	40.0	39.0	40.0	36.0	40.0
14	38.740585422293336	40.0	39.0	40.0	36.0	40.0
15	38.72557226061913	40.0	39.0	40.0	36.0	40.0
16	38.52497870154737	40.0	39.0	40.0	36.0	40.0
17	38.331733090653955	40.0	39.0	40.0	35.0	40.0
18	38.484572771666926	40.0	39.0	40.0	35.0	40.0
19	38.440001255684564	40.0	39.0	40.0	35.0	40.0
20	38.34330761121762	40.0	39.0	40.0	35.0	40.0
21	38.32770903875693	40.0	38.0	40.0	35.0	40.0
22	38.16248216618676	40.0	38.0	40.0	34.0	40.0
23	38.07619754262675	40.0	38.0	40.0	34.0	40.0
24	38.12435714488112	40.0	38.0	40.0	34.0	40.0
25	37.98731672291357	40.0	38.0	40.0	34.0	40.0
26	37.94895451674627	40.0	38.0	40.0	34.0	40.0
27	37.54449999374315	40.0	38.0	40.0	33.0	40.0
28	37.71655150471428	40.0	38.0	40.0	33.0	40.0
29	37.75609618313606	40.0	38.0	40.0	33.0	40.0
30	37.6682738134536	40.0	38.0	40.0	33.0	40.0
31	37.5846723346513	40.0	38.0	40.0	33.0	40.0
32	37.49481630974588	40.0	38.0	40.0	33.0	40.0
33	37.33592107914653	40.0	37.0	40.0	32.0	40.0
34	37.13583317403543	40.0	37.0	40.0	31.0	40.0
35	37.141657687730955	40.0	37.0	40.0	31.0	40.0
36	37.053159650439284	40.0	37.0	40.0	31.0	40.0
37	36.702367447247944	39.0	36.0	40.0	30.0	40.0
38	36.70210660143436	39.0	36.0	40.0	30.0	40.0
39	36.64656560923068	39.0	36.0	40.0	30.0	40.0
40	36.50761436762043	39.0	35.0	40.0	30.0	40.0
41	36.635657299087235	39.0	36.0	40.0	30.0	40.0
42	36.594482999570076	39.0	36.0	40.0	30.0	40.0
43	36.515737014200745	39.0	35.0	40.0	30.0	40.0
44	36.269130419023234	39.0	35.0	40.0	30.0	40.0
45	36.229736012817476	39.0	35.0	40.0	29.0	40.0
46	36.0651811040905	39.0	35.0	40.0	29.0	40.0
47	35.886305601978485	39.0	35.0	40.0	28.0	40.0
48	30.597845515703032	38.0	28.0	40.0	3.0	40.0
49	31.939505885967446	37.0	28.0	40.0	12.0	40.0
50	33.54323726123834	37.0	30.0	40.0	22.0	40.0
51	34.06230000562397	37.0	32.0	40.0	25.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	92.0
9	281.0
10	410.0
11	523.0
12	531.0
13	574.0
14	664.0
15	891.0
16	1459.0
17	2867.0
18	5981.0
19	11380.0
20	19970.0
21	33072.0
22	50197.0
23	71614.0
24	96723.0
25	125429.0
26	159321.0
27	198317.0
28	244915.0
29	299669.0
30	366819.0
31	450095.0
32	561862.0
33	722401.0
34	973680.0
35	1418787.0
36	2317222.0
37	4174891.0
38	5847204.0
39	9651685.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.41347333712772	38.87068290110024	15.102001716533742	20.6138420452383
2	28.844376093779644	29.63956027738978	26.157265070120562	15.358798558710014
3	24.78398773485293	24.855384117934815	26.204551015097987	24.15607713211427
4	21.5576731028771	29.78093626314891	24.170334833394534	24.491055800579456
5	22.385083646858924	31.010597373591185	23.250046063342598	23.354272916207293
6	24.88522999955555	25.189571690742586	27.83438426795532	22.090814041746548
7	21.72043384261195	26.384931612657127	28.051504067022776	23.843130477708147
8	24.142513437478918	25.050338855037186	27.171323846801883	23.635823860682017
9	25.3022812465884	25.413430186455493	26.597855008850924	22.686433558105186
10	22.162264363168717	26.675080328572232	29.30975609370197	21.85289921455708
11	21.98987023586014	29.71807652138842	26.442257280705046	21.849795962046397
12	22.118901533474205	25.67301024699013	29.42872609290944	22.779362126626225
13	22.406993400680026	26.308774272073332	25.748851149310966	25.53538117793568
14	22.35120677327472	29.353014642605274	25.758283167080986	22.537495417039022
15	22.474463072589643	28.85486170712977	25.58680599155714	23.083869228723447
16	23.400426156038872	25.63625306603506	25.32593500674517	25.637385771180902
17	26.439384165112884	25.454980687916645	25.534180150892148	22.571454996078323
18	22.786456816317514	25.78296175570304	25.24831413919515	26.182267288784296
19	23.449736586721418	28.81088757624544	25.13464807678173	22.604727760251414
20	26.139889732772204	25.884869259935765	25.418374534314346	22.55686647297768
21	23.271265406408133	28.6355376278896	25.28135676644972	22.811840199252543
22	26.332305771992136	25.58964674414514	25.524751729011474	22.55329575485125
23	22.779930277143823	25.900752303203088	28.74544239004094	22.573875029612147
24	22.838406629784348	28.579639527914384	25.365133796570184	23.216820045731083
25	23.161641123626246	25.67716709508092	25.286711045694695	25.874480735598137
26	22.92599170672847	27.477316051201438	27.010278346287887	22.586413895782208
27	23.331608025622295	25.269296153563463	28.636062627734983	22.763033193079266
28	25.171563476868293	25.604098623450405	26.440779370181417	22.783558529499885
29	25.096830108467845	25.601283042087868	26.66468101656655	22.637205832877736
30	25.07889740826994	25.334647846644813	26.589972819391566	22.99648192569368
31	24.479894160031172	25.915905380931974	26.479467543718467	23.12473291531839
32	23.884458034029482	26.215068991452284	26.861717773603672	23.038755200914565
33	23.91056059483489	25.738984028929362	26.884458177865056	23.46599719837069
34	23.963470510758686	25.949562905265722	26.83018182398754	23.25678475998805
35	23.592061887269445	26.10265070264397	27.015837591225917	23.289449818860668
36	23.72444455375948	25.769872718462146	26.951262610244576	23.5544201175338
37	24.183308802176175	25.852506255768702	26.738177399029944	23.226007543025176
38	23.837571232770117	26.081708243058532	26.943071174300954	23.137649349870397
39	23.8797474189785	25.77236826567236	26.886277697877116	23.46160661747202
40	23.817290416825426	26.106872276743097	26.63285379991292	23.442983506518555
41	23.712319214864888	26.2232532356172	26.885173759846086	23.17925378967183
42	23.767936835470657	25.900860179883644	26.870020682117197	23.4611823025285
43	23.887910087807303	26.087562350923417	26.672494884128216	23.35203267714106
44	23.973700815964825	25.919076955340348	26.902495158854165	23.204727069840658
45	23.830314728057992	25.644221556838858	26.852292947612348	23.6731707674908
46	24.028315183441418	25.88994665570064	26.579555527939124	23.502182632918814
47	23.7720972794508	25.906221650907323	26.884648760000708	23.43703230964117
48	21.12433247708016	35.55712480166872	23.460660898572474	19.857881822678642
49	23.929176514009658	26.084045571137267	26.49184819075704	23.49492972409604
50	23.840789553740063	26.061211673752727	26.828250831405576	23.269747941101635
51	23.892422928943933	25.749667416193844	26.673444198917117	23.684465455945105
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	24281.0
1	15263.5
2	6246.0
3	4974.5
4	3703.0
5	3838.0
6	3973.0
7	4493.0
8	5013.0
9	6208.5
10	7404.0
11	10155.5
12	12907.0
13	17370.0
14	21833.0
15	28473.5
16	35114.0
17	47788.5
18	60463.0
19	80566.0
20	100669.0
21	133890.5
22	167112.0
23	360938.5
24	554765.0
25	461875.5
26	405236.0
27	441486.0
28	490940.5
29	540395.0
30	596360.5
31	652326.0
32	716043.0
33	779760.0
34	857294.0
35	934828.0
36	1025780.5
37	1116733.0
38	1197328.5
39	1277924.0
40	1379120.0
41	1480316.0
42	1569602.0
43	1658888.0
44	1753019.5
45	1847151.0
46	1918376.5
47	1989602.0
48	2006786.0
49	2023970.0
50	2040069.0
51	2056168.0
52	2024813.0
53	1993458.0
54	1911120.5
55	1828783.0
56	1708577.0
57	1588371.0
58	1445435.5
59	1302500.0
60	1148609.0
61	994718.0
62	857496.5
63	720275.0
64	612048.0
65	503821.0
66	404902.5
67	305984.0
68	249531.0
69	193078.0
70	146971.0
71	100864.0
72	75398.5
73	49933.0
74	37735.5
75	19557.0
76	13576.0
77	10460.5
78	7345.0
79	5804.0
80	4263.0
81	3235.0
82	2207.0
83	1689.0
84	1171.0
85	921.5
86	672.0
87	540.5
88	409.0
89	352.0
90	295.0
91	229.0
92	163.0
93	107.5
94	52.0
95	41.0
96	30.0
97	20.0
98	10.0
99	5.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.7809532E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.609045821445704
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.8233148367177	32.29003303823632
2	16.82100389523286	16.01663890421716
3	6.283277633017555	8.974225582175931
4	2.9357531900086693	5.5907363257431255
5	1.6057922678912526	3.822511883087893
6	0.9806463083881963	2.8012581018411127
7	0.6842035549573295	2.2801994879411684
8	0.47945112906536996	1.826096861825366
9	0.35249713456902965	1.5103847008462723
>10	1.887753749027464	16.16973750028674
>50	0.10762822235590083	3.4382570536015677
>100	0.03580651698819163	2.9788716080262274
>500	0.0022408198792690476	0.7221297612457837
>1k	5.844168998162996E-4	0.40227604522116744
>5k	7.72083356632354E-6	0.035728923650576554
>10k+	3.860416783161769E-5	1.1409142220535546
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	237908	0.8554908439307788	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.708901250118125E-5	0.0	0.0	0.0	1.797944675947801E-5
2	9.708901250118125E-5	0.0	0.0	0.0	1.797944675947801E-5
3	9.708901250118125E-5	0.0	0.0	0.0	1.797944675947801E-5
4	1.0068490185307685E-4	0.0	0.0	0.0	1.797944675947801E-5
5	1.0428079120497245E-4	0.0	0.0	3.595889351895602E-6	2.5171225463269213E-5
6	1.0787668055686806E-4	0.0	0.0	3.595889351895602E-6	4.315067222274722E-5
7	1.2585612731634606E-4	0.0	0.0	3.595889351895602E-6	4.315067222274722E-5
8	2.049656930580493E-4	0.0	0.0	3.595889351895602E-6	4.674656157464282E-5
9	3.775683819490382E-4	0.0	0.0	3.595889351895602E-6	5.0342450926538425E-5
10	7.587326532499719E-4	0.0	0.0	3.595889351895602E-6	5.0342450926538425E-5
11	0.0013232872814975816	0.0	0.0	3.595889351895602E-6	6.113011898222523E-5
12	0.0020712322666918666	0.0	0.0	7.191778703791204E-6	6.832189768601643E-5
13	0.003027738834296097	0.0	0.0	1.0787668055686805E-5	8.630134444549444E-5
14	0.004239553545884915	0.0	0.0	1.0787668055686805E-5	8.630134444549444E-5
15	0.005440580589418045	0.0	3.595889351895602E-6	1.0787668055686805E-5	8.630134444549444E-5
16	0.007198970482494995	0.0	3.595889351895602E-6	1.0787668055686805E-5	1.0068490185307685E-4
17	0.00917311373668568	0.0	3.595889351895602E-6	1.0787668055686805E-5	1.1506845926065926E-4
18	0.011158044658932052	0.0	3.595889351895602E-6	1.0787668055686805E-5	1.2226023796445046E-4
19	0.013696742541370347	0.0	3.595889351895602E-6	1.0787668055686805E-5	1.4383557407582408E-4
20	0.016519515682608395	0.0	3.595889351895602E-6	1.0787668055686805E-5	1.5462324213151089E-4
21	0.01964793941875757	0.0	3.595889351895602E-6	1.0787668055686805E-5	1.582191314834065E-4
22	0.022715233035924518	0.0	3.595889351895602E-6	1.0787668055686805E-5	1.7260268889098888E-4
23	0.025703417087349762	3.595889351895602E-6	3.595889351895602E-6	1.0787668055686805E-5	1.7979446759478008E-4
24	0.029248963988318825	3.595889351895602E-6	3.595889351895602E-6	1.0787668055686805E-5	1.869862462985713E-4
25	0.03333749018142412	3.595889351895602E-6	3.595889351895602E-6	1.0787668055686805E-5	1.977739143542581E-4
26	0.037145537005081566	3.595889351895602E-6	3.595889351895602E-6	1.0787668055686805E-5	2.049656930580493E-4
27	0.04105067284124019	3.595889351895602E-6	3.595889351895602E-6	1.0787668055686805E-5	2.1934925046563172E-4
28	0.046084917933894035	3.595889351895602E-6	3.595889351895602E-6	1.0787668055686805E-5	2.409245865770053E-4
29	0.05055101250894837	3.595889351895602E-6	3.595889351895602E-6	1.0787668055686805E-5	2.445204759289009E-4
30	0.05522207277706076	3.595889351895602E-6	3.595889351895602E-6	1.0787668055686805E-5	2.6249992268837896E-4
31	0.06035340688221578	3.595889351895602E-6	3.595889351895602E-6	1.0787668055686805E-5	2.6969170139217016E-4
32	0.06491299458041941	3.595889351895602E-6	3.595889351895602E-6	1.0787668055686805E-5	2.7328759074406574E-4
33	0.07046145185039432	3.595889351895602E-6	3.595889351895602E-6	1.4383557407582408E-5	2.840752587997525E-4
34	0.07585888176758962	3.595889351895602E-6	3.595889351895602E-6	1.4383557407582408E-5	2.8767114815164815E-4
35	0.08146487326719486	7.191778703791204E-6	3.595889351895602E-6	1.797944675947801E-5	3.0205470555923057E-4
36	0.08702411820522546	7.191778703791204E-6	3.595889351895602E-6	1.797944675947801E-5	3.0924648426302177E-4
37	0.09236760978214233	7.191778703791204E-6	3.595889351895602E-6	6.832189768601643E-5	3.1284237361491735E-4
38	0.09810664918776771	7.191778703791204E-6	3.595889351895602E-6	6.832189768601643E-5	3.2003415231870856E-4
39	0.10370544890866916	7.191778703791204E-6	3.595889351895602E-6	1.0428079120497245E-4	3.308218203743954E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAGTAC	109550	0.0	35.981514	19
AAGCAGT	114795	0.0	35.372402	1
TCAACGC	114205	0.0	35.25568	12
CAACGCA	114080	0.0	35.158222	13
CAGAGTA	113855	0.0	34.951035	18
ACGCAGA	115250	0.0	34.756397	15
ATCAACG	116090	0.0	34.677406	11
AACGCAG	115655	0.0	34.650253	14
CGCAGAG	116915	0.0	34.18252	16
GAGTACT	45020	0.0	33.894936	20
GTGGTAT	119210	0.0	33.85664	6
GGTATCA	120105	0.0	33.748596	8
TGGTATC	119795	0.0	33.725117	7
GAGTACA	72095	0.0	33.702404	20
AGTGGTA	119930	0.0	33.670265	5
GTACATG	71075	0.0	33.651073	22
GTATCAA	121855	0.0	33.33224	9
ACATGGG	71740	0.0	33.26701	24
TACATGG	72840	0.0	32.85729	23
GTACTTT	46045	0.0	32.759254	22
>>END_MODULE