FastQCFastQC Report
Thu 26 May 2016
SRR805444_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805444_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28051985
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5712972.036565326838725No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT529090.18861053861250818No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG370720.13215464075002178No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC333590.11891850077632653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC2487500.042.74321712
CAACGCA2494750.042.72452513
AAGCAGT2478150.042.723811
ATCAACG2496200.042.5906411
AACGCAG2506300.042.51236714
ACGCAGA2506350.042.48997515
GTATCAA2503550.042.4431349
GTGGTAT2498950.042.429426
GGTATCA2508000.042.37598
AGTGGTA2507500.042.36915
TATCAAC2515850.042.24815410
CGCAGAG2524050.042.21251716
TGGTATC2518350.042.2044227
AGAGTAC2505550.041.98000319
CAGAGTA2543100.041.5229418
AGCAGTG2572150.041.3986362
GCAGTGG2576350.041.297093
CAGTGGT2575250.041.280664
GCAGAGT2577000.041.23864717
GAGTACA1698150.040.97606720