##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805444_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28051985
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.250994537463214	37.0	37.0	37.0	35.0	37.0
2	36.31782831054558	37.0	37.0	37.0	35.0	37.0
3	38.139406819160925	39.0	39.0	39.0	37.0	39.0
4	38.09454400463996	39.0	39.0	39.0	37.0	39.0
5	38.08428761814895	39.0	39.0	39.0	37.0	39.0
6	37.72381783321216	39.0	38.0	39.0	35.0	39.0
7	37.70983126506021	39.0	37.0	39.0	35.0	39.0
8	38.793145832638935	40.0	39.0	40.0	36.0	40.0
9	38.76217811324226	40.0	39.0	40.0	36.0	40.0
10	38.63520160872751	40.0	38.0	40.0	36.0	40.0
11	38.54593437861884	40.0	38.0	40.0	36.0	40.0
12	38.46685484111018	40.0	38.0	40.0	36.0	40.0
13	38.53215239491965	40.0	38.0	40.0	36.0	40.0
14	38.420840913753516	40.0	38.0	40.0	35.0	40.0
15	38.363415886611946	40.0	38.0	40.0	35.0	40.0
16	38.24705257043307	40.0	38.0	40.0	35.0	40.0
17	37.98417926574537	40.0	38.0	40.0	34.0	40.0
18	37.9414376201898	40.0	38.0	40.0	34.0	40.0
19	37.84550455163868	39.0	37.0	40.0	34.0	40.0
20	37.55205505064971	39.0	37.0	40.0	34.0	40.0
21	37.209898550851214	38.0	36.0	40.0	33.0	40.0
22	37.28168904981234	39.0	36.0	40.0	33.0	40.0
23	37.00012722807317	38.0	36.0	40.0	33.0	40.0
24	36.772572315292486	38.0	36.0	40.0	32.0	40.0
25	36.47470922289457	38.0	36.0	40.0	32.0	40.0
26	36.42678947675182	38.0	35.0	40.0	31.0	40.0
27	36.19764672624771	38.0	35.0	39.0	31.0	40.0
28	35.94121849131176	38.0	35.0	39.0	31.0	40.0
29	35.454291024325016	37.0	34.0	39.0	30.0	40.0
30	35.27756435061547	37.0	34.0	39.0	30.0	40.0
31	35.01718192135066	37.0	33.0	38.0	29.0	40.0
32	34.7711319894118	36.0	33.0	38.0	29.0	40.0
33	34.46016230223993	36.0	33.0	38.0	28.0	40.0
34	34.168707882882444	36.0	32.0	38.0	27.0	39.0
35	33.87337049410229	36.0	32.0	38.0	27.0	39.0
36	33.72579017135507	35.0	31.0	38.0	27.0	39.0
37	33.5819012451347	35.0	31.0	38.0	27.0	39.0
38	33.288643210097256	35.0	31.0	38.0	26.0	39.0
39	33.01832882771041	35.0	31.0	38.0	26.0	39.0
40	32.77647649533536	34.0	31.0	37.0	25.0	38.0
41	32.442120833873254	34.0	30.0	37.0	24.0	38.0
42	32.12539501215333	34.0	30.0	37.0	24.0	38.0
43	31.798965527751424	33.0	29.0	37.0	24.0	38.0
44	31.423610414735357	33.0	29.0	36.0	23.0	38.0
45	30.94863835839068	33.0	28.0	36.0	22.0	38.0
46	30.69069825183494	32.0	28.0	36.0	22.0	38.0
47	30.309653880108662	32.0	27.0	35.0	21.0	38.0
48	29.87421446289808	31.0	27.0	35.0	20.0	37.0
49	29.428135798589654	31.0	26.0	35.0	19.0	37.0
50	29.21138546880016	31.0	26.0	34.0	18.0	37.0
51	30.485928785431764	32.0	28.0	35.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	77.0
8	340.0
9	702.0
10	798.0
11	771.0
12	860.0
13	904.0
14	1069.0
15	1432.0
16	2211.0
17	3699.0
18	7059.0
19	13469.0
20	24232.0
21	40832.0
22	62853.0
23	91919.0
24	127168.0
25	166927.0
26	214272.0
27	268811.0
28	332191.0
29	408336.0
30	505438.0
31	644836.0
32	865731.0
33	1279992.0
34	2255471.0
35	4971517.0
36	8923060.0
37	6017242.0
38	812316.0
39	5449.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.2694001868317	35.218894491780176	28.997210001359974	21.514495320028153
2	28.333549301413075	41.14506691772436	12.30434851580022	18.217035265062346
3	34.67722872374272	25.841515315226353	17.861848279185946	21.619407681844972
4	23.205416657680374	23.396583165148563	19.61840490075836	33.77959527641271
5	21.328116352550452	32.284934559889436	23.26661018819167	23.120338899368438
6	31.259852021167127	23.274028558050347	21.860435188454577	23.605684232327945
7	23.26215774035242	22.631820885402583	31.087404331636424	23.01861704260857
8	30.45328877795992	24.63983921280437	22.98511495710553	21.92175705213018
9	30.71144876200383	24.1127962958771	23.0409826612983	22.13477228082077
10	22.048457533397368	24.407549055797656	31.489158432103824	22.05483497870115
11	21.980697622645955	32.620461617956806	23.330680520469407	22.068160238927835
12	21.851444737333207	24.017462578851372	31.87110644754729	22.25998623626813
13	21.83118948623422	24.210814314922814	23.605634324986273	30.352361873856697
14	22.26998552865332	32.65263759409539	23.237293189768923	21.840083687482366
15	22.0164491033344	32.35074451950548	23.3032172233088	22.329589153851323
16	22.241573992000923	23.758500512530574	23.527033826661466	30.47289166880704
17	30.599560066783155	23.761669628726807	23.431507609889284	22.207262694600757
18	22.09450418571092	23.87523378470365	23.469102097409504	30.561159932175926
19	22.275835382059416	32.14547918801468	23.498148170263175	22.08053725966273
20	30.75681097077444	23.932851810665092	23.41611832460341	21.89421889395706
21	22.304877890102965	32.077473305365025	23.44527490657078	22.172373897961233
22	30.363391396366424	23.670193036250375	23.801581242824707	22.164834324558495
23	22.265222942333672	23.885913955821664	31.777523052290242	22.071340049554426
24	22.26557942334562	31.895439841422984	23.45501753262737	22.383963202604022
25	22.0929641877393	23.84967766095697	23.571811406572476	30.485546744731256
26	22.407854560024898	29.267839691201885	26.2436615448069	22.080644203966315
27	22.50725216058685	23.67954353319382	31.634338889030488	22.178865417188838
28	27.823963259640983	23.67391113320501	26.3011619320344	22.200963675119603
29	28.02695067746543	23.77003980288739	26.13057150857595	22.07243801107123
30	27.819047386486197	23.757994309493608	26.159179109784926	22.263779194235276
31	25.869431343272144	24.538744762625534	26.72523530865998	22.86658858544235
32	24.663962282883013	25.394684190797907	26.871217847863527	23.070135678455554
33	24.335422252649856	24.86244021590629	27.286407717671317	23.515729813772538
34	23.796811526884817	24.79365363984046	27.619414454984202	23.79012037829052
35	23.80118911371156	24.954062965597622	27.59544823655082	23.649299684139997
36	23.825226628347334	24.9224288405972	27.667974298432004	23.584370232623467
37	24.081728975685678	24.759242527756946	27.664516432616086	23.494512063941286
38	24.238673306006685	24.846295190875082	27.52727837263566	23.387753130482565
39	24.209734177456603	24.78853100769874	27.544378766778895	23.457356048065762
40	23.776189100343522	25.09645930582096	27.391320079488136	23.73603151434738
41	24.132520390268283	25.324974328911125	27.186842571033743	23.35566270978685
42	24.057320720797478	25.23325532934657	27.347619072233215	23.361804877622742
43	23.925379968654624	25.00496488929393	27.751544142063388	23.318110999988058
44	24.21425079187801	24.897179290520796	27.33758413174683	23.55098578585437
45	24.247781395862003	24.589347242271803	27.425339062458505	23.737532299407686
46	23.893681677072053	24.704269590904175	27.374868480786652	24.027180251237123
47	24.02329460820687	24.81544888891107	27.31198523027871	23.84927127260335
48	23.90278976692737	24.739115609822264	27.39078179316009	23.967312830090275
49	23.759338242908655	25.145250861926527	27.317817259634214	23.777593635530607
50	24.231315537920047	25.055599452231274	27.074333598852274	23.638751410996406
51	24.043984766140436	24.927009621600753	27.269111258971513	23.759894353287297
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3312.0
1	2077.5
2	843.0
3	708.0
4	573.0
5	656.5
6	740.0
7	906.5
8	1073.0
9	1477.0
10	1881.0
11	3139.5
12	4398.0
13	6530.0
14	8662.0
15	12141.5
16	15621.0
17	22793.0
18	29965.0
19	41481.5
20	52998.0
21	74746.5
22	96495.0
23	427477.0
24	758459.0
25	534913.5
26	317539.5
27	323711.0
28	358398.5
29	393086.0
30	438614.0
31	484142.0
32	546669.5
33	609197.0
34	684836.5
35	760476.0
36	860161.5
37	959847.0
38	1052909.5
39	1145972.0
40	1253237.5
41	1360503.0
42	1461981.5
43	1563460.0
44	1675853.5
45	1788247.0
46	1890329.0
47	1992411.0
48	2048600.5
49	2104790.0
50	2150612.5
51	2196435.0
52	2199455.0
53	2202475.0
54	2167073.5
55	2131672.0
56	2003918.5
57	1876165.0
58	1703523.0
59	1530881.0
60	1349557.5
61	1168234.0
62	1000925.0
63	833616.0
64	698248.0
65	562880.0
66	453746.5
67	344613.0
68	278945.5
69	213278.0
70	162162.5
71	111047.0
72	82950.5
73	54854.0
74	41370.0
75	20639.0
76	13392.0
77	9688.0
78	5984.0
79	4701.5
80	3419.0
81	2600.5
82	1782.0
83	1279.5
84	777.0
85	521.5
86	266.0
87	162.5
88	59.0
89	40.5
90	22.0
91	15.0
92	8.0
93	7.5
94	7.0
95	5.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.8051985E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.24997371326541
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.37273857348724	32.54737309154284
2	15.556361120556462	14.389625857996007
3	5.611084911771487	7.785375890169943
4	2.602991517283673	4.815531570008909
5	1.4627674334589174	3.382647767304782
6	0.8392427436536531	2.328897289981812
7	0.5820858929191436	1.8845020072461114
8	0.43046620553797477	1.592724055246435
9	0.3154451985604917	1.3130398927258677
>10	2.037858508784856	17.458533068238406
>50	0.13132532175080647	4.173352014564314
>100	0.055269434267660816	4.299303066763471
>500	0.001602204071945981	0.48141898709967895
>1k	6.290511672486048E-4	0.5596815645802371
>5k	6.594136421581418E-5	0.17539321364806693
>10k+	6.594136418907253E-5	2.8126006628832037
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	571297	2.036565326838725	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT	52909	0.18861053861250818	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG	37072	0.13215464075002178	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC	33359	0.11891850077632653	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.2777721433973386E-5	7.129620238995565E-6	0.0	0.0	1.7824050597488914E-5
2	4.990734167296895E-5	7.129620238995565E-6	0.0	3.5648101194977823E-6	4.2777721433973386E-5
3	5.347215179246674E-5	7.129620238995565E-6	0.0	7.129620238995565E-6	4.2777721433973386E-5
4	5.703696191196452E-5	7.129620238995565E-6	0.0	1.7824050597488914E-5	5.347215179246674E-5
5	7.129620238995566E-5	7.129620238995565E-6	0.0	2.4953670836484476E-5	8.199063274844899E-5
6	7.842582262895121E-5	7.129620238995565E-6	0.0	2.4953670836484476E-5	8.555544286794677E-5
7	8.199063274844899E-5	1.0694430358493347E-5	0.0	4.2777721433973386E-5	8.912025298744457E-5
8	1.033794934654357E-4	1.0694430358493347E-5	0.0	4.2777721433973386E-5	1.033794934654357E-4
9	1.0694430358493348E-4	1.0694430358493347E-5	0.0	4.634253155347117E-5	1.033794934654357E-4
10	1.0694430358493348E-4	1.425924047799113E-5	0.0	4.990734167296895E-5	1.1407392382392903E-4
11	1.1050911370443126E-4	1.425924047799113E-5	0.0	5.347215179246674E-5	1.425924047799113E-4
12	1.1407392382392903E-4	1.425924047799113E-5	0.0	5.703696191196452E-5	1.4972202501890687E-4
13	1.1407392382392903E-4	1.425924047799113E-5	0.0	6.0601772031462303E-5	1.4972202501890687E-4
14	1.1763873394342681E-4	1.425924047799113E-5	0.0	6.0601772031462303E-5	1.7111088573589354E-4
15	1.2120354406292461E-4	1.425924047799113E-5	0.0	6.0601772031462303E-5	1.7467569585539135E-4
16	1.390275946604135E-4	1.425924047799113E-5	0.0	8.199063274844899E-5	1.818053160943869E-4
17	1.6754607561639577E-4	1.425924047799113E-5	0.0	8.555544286794677E-5	2.0319417681137359E-4
18	1.6754607561639577E-4	1.7824050597488914E-5	0.0	8.555544286794677E-5	2.245830375283603E-4
19	1.9606455657237803E-4	1.7824050597488914E-5	0.0	8.912025298744457E-5	2.6023113872333814E-4
20	2.4240708812584921E-4	1.7824050597488914E-5	0.0	9.624987322644012E-5	2.9587923991831593E-4
21	2.78055189320827E-4	1.7824050597488914E-5	0.0	1.033794934654357E-4	3.1013848039630705E-4
22	3.030088601573115E-4	1.7824050597488914E-5	0.0	1.0694430358493348E-4	3.2796253099379597E-4
23	3.208329107548004E-4	1.7824050597488914E-5	0.0	1.1050911370443126E-4	3.422217714717871E-4
24	3.3509215123279153E-4	1.7824050597488914E-5	0.0	1.2476835418242239E-4	3.636106321887738E-4
25	3.6717544230827157E-4	1.7824050597488914E-5	0.0	1.2476835418242239E-4	3.7430506254726713E-4
26	4.09953163742245E-4	1.7824050597488914E-5	0.0	1.2476835418242239E-4	3.7430506254726713E-4
27	4.42036454817725E-4	1.7824050597488914E-5	0.0	1.2833316430192017E-4	3.885643030252583E-4
28	5.026382268491874E-4	1.7824050597488914E-5	0.0	1.3189797442141794E-4	4.135179738617428E-4
29	5.846288595976364E-4	1.7824050597488914E-5	0.0	1.3546278454091572E-4	4.242124042202361E-4
30	6.523602518680941E-4	1.7824050597488914E-5	0.0	1.3546278454091572E-4	4.456012649372228E-4
31	7.307860744970454E-4	1.7824050597488914E-5	0.0	1.3546278454091572E-4	4.527308851762184E-4
32	8.234711376039878E-4	1.7824050597488914E-5	0.0	1.3546278454091572E-4	4.5986050541521394E-4
33	8.947673399939434E-4	1.7824050597488914E-5	0.0	1.390275946604135E-4	4.812493661322006E-4
34	0.0010409245548933526	1.7824050597488914E-5	0.0	1.4972202501890687E-4	4.883789863711962E-4
35	0.0011015263269248147	1.7824050597488914E-5	0.0	1.5328683513840465E-4	4.990734167296895E-4
36	0.0012405539215852282	1.7824050597488914E-5	0.0	1.6398126549689799E-4	5.204622774466763E-4
37	0.0013225445543336772	1.7824050597488914E-5	0.0	1.6398126549689799E-4	5.275918976856718E-4
38	0.0014080999972016241	1.7824050597488914E-5	0.0	1.6754607561639577E-4	5.418511381636629E-4
39	0.001522173921025553	2.1388860716986693E-5	0.0	1.7111088573589354E-4	5.454159482831607E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	248750	0.0	42.743217	12
CAACGCA	249475	0.0	42.724525	13
AAGCAGT	247815	0.0	42.72381	1
ATCAACG	249620	0.0	42.59064	11
AACGCAG	250630	0.0	42.512367	14
ACGCAGA	250635	0.0	42.489975	15
GTATCAA	250355	0.0	42.443134	9
GTGGTAT	249895	0.0	42.42942	6
GGTATCA	250800	0.0	42.3759	8
AGTGGTA	250750	0.0	42.3691	5
TATCAAC	251585	0.0	42.248154	10
CGCAGAG	252405	0.0	42.212517	16
TGGTATC	251835	0.0	42.204422	7
AGAGTAC	250555	0.0	41.980003	19
CAGAGTA	254310	0.0	41.52294	18
AGCAGTG	257215	0.0	41.398636	2
GCAGTGG	257635	0.0	41.29709	3
CAGTGGT	257525	0.0	41.28066	4
GCAGAGT	257700	0.0	41.238647	17
GAGTACA	169815	0.0	40.976067	20
>>END_MODULE