FastQCFastQC Report
Thu 26 May 2016
SRR805443_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805443_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20142166
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT239680.11899415385614437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT185350.024.2662561
TCAACGC201000.022.4664212
CAACGCA204750.022.1318713
ATCAACG211750.021.30460511
AACGCAG214500.021.22028214
ACGCAGA215050.021.01953115
GTATCAA217650.020.7891129
GGTATCA216900.020.7572638
AGTGGTA221000.020.5350695
GTGGTAT223550.020.1397916
TGGTATC225700.019.9977847
CGCAGAG230550.019.6942116
TATCAAC231100.019.68628510
AGAGTAC234700.019.19258719
AGCAGTG254350.017.7806192
GCAGTGG256800.017.7599323
CAGTGGT260200.017.597044
CAGAGTA260250.017.5158518
GCAGAGT272400.016.71806117
GAGTACA182700.015.75123220
Produced by FastQC (version 0.11.5)