##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805443_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20142166
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.596259508535475	37.0	35.0	37.0	33.0	37.0
2	35.75560731651204	37.0	35.0	37.0	35.0	37.0
3	37.56524050094712	39.0	38.0	39.0	35.0	39.0
4	37.63415682305468	39.0	38.0	39.0	35.0	39.0
5	37.69199275787917	39.0	38.0	39.0	35.0	39.0
6	37.28573168347436	39.0	37.0	39.0	34.0	39.0
7	37.37852959805812	39.0	37.0	39.0	34.0	39.0
8	38.334426595431694	40.0	38.0	40.0	35.0	40.0
9	38.27417622315296	40.0	38.0	40.0	34.0	40.0
10	38.277257917544716	40.0	38.0	40.0	35.0	40.0
11	38.14105444270492	40.0	38.0	40.0	34.0	40.0
12	37.981757374057985	40.0	38.0	40.0	34.0	40.0
13	38.012129082840445	40.0	38.0	40.0	34.0	40.0
14	37.90168947073517	40.0	38.0	40.0	34.0	40.0
15	37.83407236341911	40.0	38.0	40.0	34.0	40.0
16	37.718757605314146	40.0	37.0	40.0	34.0	40.0
17	37.56953144959683	39.0	37.0	40.0	33.0	40.0
18	37.35167220844074	39.0	37.0	40.0	33.0	40.0
19	37.213234415802155	39.0	36.0	40.0	32.0	40.0
20	36.922248630062924	38.0	36.0	40.0	32.0	40.0
21	36.743440849410135	38.0	36.0	40.0	32.0	40.0
22	36.56446491405145	38.0	36.0	40.0	31.0	40.0
23	36.40442065664636	38.0	36.0	40.0	31.0	40.0
24	36.060469613843914	38.0	35.0	40.0	30.0	40.0
25	35.84802761530215	38.0	35.0	39.0	30.0	40.0
26	35.63355127745447	38.0	34.0	39.0	30.0	40.0
27	35.40678400724133	38.0	34.0	39.0	29.0	40.0
28	35.14938035958993	37.0	34.0	39.0	29.0	40.0
29	34.76134230052518	37.0	33.0	39.0	27.0	40.0
30	34.605218028686686	37.0	33.0	38.0	27.0	40.0
31	34.334536811979405	36.0	33.0	38.0	27.0	40.0
32	34.07233184355645	36.0	32.0	38.0	27.0	39.0
33	33.81340169671921	36.0	32.0	38.0	26.0	39.0
34	33.464162195863146	35.0	31.0	38.0	25.0	39.0
35	33.16886902828623	35.0	31.0	38.0	25.0	39.0
36	33.00602040515405	35.0	31.0	38.0	24.0	39.0
37	32.86628146148731	35.0	31.0	38.0	24.0	39.0
38	32.635670761525844	35.0	30.0	38.0	24.0	39.0
39	32.33528067438229	34.0	30.0	37.0	24.0	39.0
40	32.042978942781026	34.0	30.0	37.0	24.0	38.0
41	31.75959924071721	34.0	29.0	37.0	23.0	38.0
42	31.41880550483002	33.0	29.0	37.0	22.0	38.0
43	31.0819203356779	33.0	29.0	37.0	21.0	38.0
44	30.637265773700804	33.0	28.0	36.0	19.0	38.0
45	30.274260573564927	32.0	27.0	36.0	19.0	38.0
46	29.928465687354578	32.0	27.0	35.0	18.0	38.0
47	29.548967226265535	31.0	26.0	35.0	18.0	38.0
48	29.169135881414142	31.0	26.0	35.0	17.0	37.0
49	28.67437598319863	31.0	25.0	34.0	15.0	37.0
50	28.50027703078209	31.0	25.0	34.0	15.0	37.0
51	30.005946480631724	32.0	28.0	35.0	20.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	37.0
8	208.0
9	425.0
10	679.0
11	866.0
12	1208.0
13	1849.0
14	2845.0
15	4423.0
16	6793.0
17	10605.0
18	17070.0
19	26764.0
20	41476.0
21	62222.0
22	88874.0
23	121272.0
24	160424.0
25	202404.0
26	249419.0
27	302498.0
28	361692.0
29	431591.0
30	515709.0
31	627781.0
32	794390.0
33	1083403.0
34	1711013.0
35	3357580.0
36	5723743.0
37	3741944.0
38	487629.0
39	3329.0
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.51905778157126	29.20997672246371	20.583262991676268	27.687702504288765
2	25.112060937239818	23.06537439915846	29.524123671704423	22.298440991897298
3	20.45822182182393	25.39842537292166	27.53656682205876	26.606785983195653
4	21.7162791727563	28.066455216385368	26.866102682303385	23.351162928554952
5	25.496706759342562	27.260047405030818	25.329098171467756	21.914147664158858
6	24.441239338410774	25.45392089410841	27.40764821419901	22.697191553281808
7	24.102487289599342	26.543327068201105	26.748766741372304	22.60541890082725
8	22.830449317119122	25.404939071597365	27.72724641431314	24.037365196970377
9	22.790344394937467	25.25290477697384	28.366497426344317	23.59025340174438
10	23.016010294026966	26.10090195860763	27.45129793886119	23.43178980850421
11	22.941385747689697	26.567817979456628	27.053912672549714	23.436883600303958
12	23.176410123916167	25.895114755781478	27.193207522964514	23.735267597337845
13	23.30382938955026	26.286428182549983	26.37211906604285	24.037623361856912
14	23.444112217126996	26.620126157236516	26.397280213061496	23.538481412574992
15	23.379650430842442	26.368787746064648	26.32808209405086	23.92347972904205
16	23.774339859973352	26.08348079347574	26.094562024759405	24.04761732179151
17	24.29305269353852	25.90645911666104	26.170134830583763	23.63035335921668
18	23.624787920028066	25.91303239184902	26.14703900265741	24.315140685465504
19	23.971006891711646	26.31232907126274	25.99000028100255	23.72666375602306
20	24.186599395516847	25.961557461099265	26.18965606777345	23.66218707561044
21	23.82991978121916	26.215179638575115	26.170338383667378	23.784562196538346
22	24.161190013030375	25.870172055974518	26.192272469604312	23.776365461390796
23	23.648692995579523	25.99651894438761	26.763889246072146	23.590898813960724
24	23.595635146686806	26.182899098339274	26.246943849037883	23.974521905936037
25	23.749317724816684	25.924654776452545	26.131851956735936	24.194175541994838
26	23.78984464729364	26.02531922336456	26.605495158762967	23.579340970578837
27	23.84370677910211	25.612002204728128	26.73356976603211	23.810721250137647
28	24.202938254009027	25.753640397959188	26.242366387011206	23.801054961020576
29	24.36264302458832	25.702762056473965	26.407264243577377	23.527330675360336
30	24.06378241545621	25.637495987273663	26.34245492763787	23.956266669632253
31	24.11332525012454	25.85354524433966	26.15742517463117	23.875704330904632
32	23.978697226504835	25.906925799340545	26.325624562919398	23.78875241123522
33	23.88240668853588	25.7455727452549	26.308148785984585	24.06387178022463
34	24.042528494701116	25.856543928790977	26.1553797143763	23.945547862131612
35	23.97458644715767	25.88652580859477	26.295856165617938	23.843031578629628
36	23.922174010481296	25.718033502454503	26.301088969279668	24.058703517784533
37	24.0509039593855	25.874868671025748	26.083818393711976	23.990408975876775
38	24.00822235304783	25.87385090560767	26.296183836435468	23.821742904909033
39	23.978672402958054	25.690886471693265	26.30982189303772	24.020619232310963
40	24.045591720374066	25.85402682114724	26.134657017522343	23.96572444095635
41	23.988150033119577	25.819085196696324	26.366141555977645	23.826623214206457
42	23.91647948884941	25.67860378074533	26.39190343282842	24.013013297576833
43	24.05935389471023	25.78189455890692	26.117672746813824	24.041078799569025
44	24.004265479690716	25.717412913784944	26.380092389269354	23.898229217254986
45	24.02398530525466	25.549665314048152	26.39143178543956	24.034917595257628
46	24.02900959112342	25.733295019016328	26.172289514444476	24.06540587541578
47	23.97150832735665	25.7149305591067	26.365237978874767	23.948323134661884
48	23.925535118715633	25.569856787000962	26.342876927933172	24.161731166350233
49	24.24962638079738	25.685728138671877	26.126926965054302	23.93771851547644
50	23.99377504882047	25.723241482569453	26.33395534521957	23.949028123390505
51	24.036029690153484	25.606188530071694	26.26780059304446	24.089981186730363
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6347.0
1	4286.0
2	2225.0
3	1885.5
4	1546.0
5	1593.0
6	1640.0
7	1830.0
8	2020.0
9	2570.5
10	3121.0
11	4652.0
12	6183.0
13	8821.5
14	11460.0
15	15500.5
16	19541.0
17	27656.0
18	35771.0
19	47764.0
20	59757.0
21	78803.5
22	97850.0
23	139490.0
24	181130.0
25	196854.5
26	246267.5
27	279956.0
28	317490.5
29	355025.0
30	398270.0
31	441515.0
32	491412.5
33	541310.0
34	600592.5
35	659875.0
36	733495.0
37	807115.0
38	871780.5
39	936446.0
40	1007961.0
41	1079476.0
42	1143375.0
43	1207274.0
44	1270958.0
45	1334642.0
46	1387124.5
47	1439607.0
48	1472332.5
49	1505058.0
50	1520476.0
51	1535894.0
52	1518361.5
53	1500829.0
54	1442218.0
55	1383607.0
56	1296603.5
57	1209600.0
58	1112361.0
59	1015122.0
60	900020.5
61	784919.0
62	674956.5
63	564994.0
64	475499.5
65	386005.0
66	310525.5
67	235046.0
68	191800.0
69	148554.0
70	112424.5
71	76295.0
72	56196.5
73	36098.0
74	27170.5
75	13851.0
76	9459.0
77	6953.5
78	4448.0
79	3463.5
80	2479.0
81	1893.0
82	1307.0
83	927.0
84	547.0
85	355.0
86	163.0
87	108.5
88	54.0
89	37.5
90	21.0
91	15.0
92	9.0
93	6.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.0142166E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.75785267297915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.10554949991823	41.31966311266604
2	14.671293895383162	16.360796870815054
3	4.774227524294079	7.986020247806136
4	2.124313062582334	4.737885390990036
5	1.0987468253002488	3.0631881804997416
6	0.6869715822594026	2.298243616448589
7	0.4572267391259809	1.7845786810833177
8	0.32864212554402494	1.4659503374574767
9	0.24152220603302918	1.21200836231183
>10	1.410408728600757	14.037997875497615
>50	0.07636552247330612	2.880301622047201
>100	0.023979259542723366	2.3568783939347466
>500	6.443430921478819E-4	0.22087194497431448
>1k	7.905809241291112E-5	0.07374889328275047
>5k	1.9751838720476366E-5	0.06988426779559141
>10k+	9.875919360237852E-6	0.131982202389464
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	23968	0.11899415385614437	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0355308361573427E-4	0.0	0.0	0.0	1.4894128069443971E-5
2	2.0355308361573427E-4	0.0	0.0	9.929418712962647E-6	1.9858837425925295E-5
3	2.085177929722156E-4	0.0	0.0	9.929418712962647E-6	4.4682384208331915E-5
4	2.1348250232869692E-4	4.964709356481324E-6	0.0	9.929418712962647E-6	4.964709356481324E-5
5	2.2341192104165957E-4	4.964709356481324E-6	0.0	1.9858837425925295E-5	6.950593099073853E-5
6	2.2837663039814088E-4	4.964709356481324E-6	0.0	1.9858837425925295E-5	8.44000590601825E-5
7	2.2837663039814088E-4	4.964709356481324E-6	0.0	3.4752965495369264E-5	8.44000590601825E-5
8	2.2837663039814088E-4	4.964709356481324E-6	0.0	3.4752965495369264E-5	8.936476841666383E-5
9	2.3334133975462222E-4	4.964709356481324E-6	0.0	3.4752965495369264E-5	8.936476841666383E-5
10	2.3334133975462222E-4	9.929418712962647E-6	0.0	3.971767485185059E-5	1.1418831519907044E-4
11	2.3334133975462222E-4	9.929418712962647E-6	0.0	5.4611802921294565E-5	1.1915302455555177E-4
12	2.3334133975462222E-4	1.4894128069443971E-5	0.0	5.4611802921294565E-5	1.3404715262499574E-4
13	2.3830604911110354E-4	1.4894128069443971E-5	0.0	5.4611802921294565E-5	1.439765713379584E-4
14	2.4327075846758487E-4	1.9858837425925295E-5	0.0	5.4611802921294565E-5	1.7872953683332766E-4
15	2.532001771805475E-4	1.9858837425925295E-5	0.0	5.4611802921294565E-5	1.9362366490277162E-4
16	2.680943052499915E-4	1.9858837425925295E-5	0.0	5.4611802921294565E-5	2.1348250232869692E-4
17	2.829884333194355E-4	2.482354678240662E-5	0.0	5.4611802921294565E-5	2.2837663039814088E-4
18	2.829884333194355E-4	2.9788256138887942E-5	0.0	6.454122163425722E-5	2.3334133975462222E-4
19	2.978825613888794E-4	3.4752965495369264E-5	0.0	7.447064034721985E-5	2.3830604911110354E-4
20	3.276708175277674E-4	3.4752965495369264E-5	0.0	9.432947777314515E-5	2.4327075846758487E-4
21	3.475296549536927E-4	3.4752965495369264E-5	0.0	9.929418712962648E-5	2.482354678240662E-4
22	3.8228262044906193E-4	3.4752965495369264E-5	0.0	1.1915302455555177E-4	2.532001771805475E-4
23	4.1207087658794985E-4	3.4752965495369264E-5	0.0	1.1915302455555177E-4	2.5816488653702886E-4
24	4.2200029530091253E-4	3.4752965495369264E-5	0.0	1.2908244326851443E-4	2.730590146064728E-4
25	4.4682384208331915E-4	3.4752965495369264E-5	0.0	1.3404715262499574E-4	2.929178520323981E-4
26	4.8654151693516975E-4	3.4752965495369264E-5	0.0	1.3404715262499574E-4	2.978825613888794E-4
27	5.262591917870204E-4	3.4752965495369264E-5	0.0	1.439765713379584E-4	3.028472707453608E-4
28	6.007298321342401E-4	3.4752965495369264E-5	0.0	1.5390599005092105E-4	3.028472707453608E-4
29	6.305180882731281E-4	3.971767485185059E-5	0.0	1.7872953683332766E-4	3.127766894583234E-4
30	6.603063444120161E-4	3.971767485185059E-5	0.0	1.7872953683332766E-4	3.127766894583234E-4
31	7.347769847592359E-4	3.971767485185059E-5	0.0	1.7872953683332766E-4	3.22706108171286E-4
32	8.241417531758997E-4	4.4682384208331915E-5	0.0	1.886589555462903E-4	3.52494364310174E-4
33	9.234359403055263E-4	4.964709356481324E-5	0.0	1.886589555462903E-4	3.574590736666553E-4
34	9.730830338703395E-4	5.4611802921294565E-5	0.0	1.886589555462903E-4	3.8228262044906193E-4
35	0.001042588964861078	5.4611802921294565E-5	0.0	1.9362366490277162E-4	3.971767485185059E-4
36	0.001131953733277742	5.9576512277775884E-5	0.0	1.9362366490277162E-4	4.2696500465739384E-4
37	0.0011915302455555176	6.454122163425722E-5	0.0	2.1348250232869692E-4	4.4682384208331915E-4
38	0.0012610361765462562	6.950593099073853E-5	0.0	2.2341192104165957E-4	4.4682384208331915E-4
39	0.001380189201101808	6.950593099073853E-5	0.0	2.3334133975462222E-4	4.7661209822220707E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	18535	0.0	24.266256	1
TCAACGC	20100	0.0	22.46642	12
CAACGCA	20475	0.0	22.13187	13
ATCAACG	21175	0.0	21.304605	11
AACGCAG	21450	0.0	21.220282	14
ACGCAGA	21505	0.0	21.019531	15
GTATCAA	21765	0.0	20.789112	9
GGTATCA	21690	0.0	20.757263	8
AGTGGTA	22100	0.0	20.535069	5
GTGGTAT	22355	0.0	20.139791	6
TGGTATC	22570	0.0	19.997784	7
CGCAGAG	23055	0.0	19.69421	16
TATCAAC	23110	0.0	19.686285	10
AGAGTAC	23470	0.0	19.192587	19
AGCAGTG	25435	0.0	17.780619	2
GCAGTGG	25680	0.0	17.759932	3
CAGTGGT	26020	0.0	17.59704	4
CAGAGTA	26025	0.0	17.51585	18
GCAGAGT	27240	0.0	16.718061	17
GAGTACA	18270	0.0	15.751232	20
>>END_MODULE