FastQCFastQC Report
Thu 26 May 2016
SRR805442_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805442_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15505870
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT821450.5297671140026325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT422600.037.695221
TCAACGC434250.036.4559612
CAACGCA434700.036.3561113
AACGCAG440450.035.8610514
ATCAACG442700.035.7448611
ACGCAGA442950.035.61293815
GGTATCA447050.035.5430038
GTGGTAT445350.035.517016
AGTGGTA446100.035.4018145
GTATCAA450200.035.394279
TGGTATC452400.035.0132647
TATCAAC454700.034.8806910
CGCAGAG453150.034.7517416
AGAGTAC456100.034.0435219
CAGAGTA476950.032.95628718
GCAGTGG483300.032.8770943
AGCAGTG484150.032.8704952
CAGTGGT483100.032.816194
GCAGAGT484800.032.5247517
GTACATG312850.031.56544522
Produced by FastQC (version 0.11.5)