##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805442_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15505870
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.14994108682712	37.0	37.0	37.0	35.0	37.0
2	36.20123617700909	37.0	37.0	37.0	35.0	37.0
3	37.9520598973163	39.0	39.0	39.0	35.0	39.0
4	37.91893224952873	39.0	38.0	39.0	35.0	39.0
5	37.92090350299596	39.0	38.0	39.0	35.0	39.0
6	37.89432073143912	39.0	38.0	39.0	35.0	39.0
7	37.85660849729812	39.0	38.0	39.0	35.0	39.0
8	38.72483930279307	40.0	39.0	40.0	36.0	40.0
9	38.73605299154449	40.0	39.0	40.0	36.0	40.0
10	38.672582254333356	40.0	39.0	40.0	36.0	40.0
11	38.759507205980704	40.0	39.0	40.0	36.0	40.0
12	38.75406868495608	40.0	39.0	40.0	36.0	40.0
13	38.81955014455816	40.0	39.0	40.0	36.0	40.0
14	38.77319260383326	40.0	39.0	40.0	36.0	40.0
15	38.74779254566174	40.0	39.0	40.0	36.0	40.0
16	38.52280833000663	40.0	39.0	40.0	36.0	40.0
17	38.29340411083029	40.0	39.0	40.0	35.0	40.0
18	38.49117637385068	40.0	39.0	40.0	35.0	40.0
19	38.50029956397158	40.0	39.0	40.0	35.0	40.0
20	38.36550358025703	40.0	39.0	40.0	35.0	40.0
21	38.352974905632514	40.0	38.0	40.0	35.0	40.0
22	38.13877634728009	40.0	38.0	40.0	34.0	40.0
23	38.11061043333912	40.0	38.0	40.0	34.0	40.0
24	38.13600184962211	40.0	38.0	40.0	34.0	40.0
25	37.92160594665117	40.0	38.0	40.0	34.0	40.0
26	37.927431804858415	40.0	38.0	40.0	34.0	40.0
27	37.54811435927168	40.0	38.0	40.0	33.0	40.0
28	37.69890841339441	40.0	38.0	40.0	33.0	40.0
29	37.734449469781445	40.0	38.0	40.0	33.0	40.0
30	37.66391540751986	40.0	38.0	40.0	33.0	40.0
31	37.58427253678768	40.0	38.0	40.0	33.0	40.0
32	37.48096604705186	40.0	38.0	40.0	33.0	40.0
33	37.34094288163128	40.0	37.0	40.0	32.0	40.0
34	37.08241672347311	40.0	37.0	40.0	31.0	40.0
35	37.10216943647793	40.0	37.0	40.0	31.0	40.0
36	36.97414772599022	40.0	37.0	40.0	31.0	40.0
37	36.52099688698538	39.0	36.0	40.0	30.0	40.0
38	36.61066912079103	39.0	36.0	40.0	30.0	40.0
39	36.58261593835109	39.0	36.0	40.0	30.0	40.0
40	36.42800049271663	39.0	35.0	40.0	30.0	40.0
41	36.55705278065662	39.0	36.0	40.0	30.0	40.0
42	36.51948423403524	39.0	36.0	40.0	30.0	40.0
43	36.4456360720166	39.0	35.0	40.0	30.0	40.0
44	36.25994175109168	39.0	35.0	40.0	29.0	40.0
45	36.14335790252337	39.0	35.0	40.0	29.0	40.0
46	35.90190205386734	39.0	35.0	40.0	28.0	40.0
47	35.71583097239949	38.0	35.0	40.0	28.0	40.0
48	29.977211146488393	37.0	26.0	40.0	3.0	40.0
49	31.33874487532786	37.0	26.0	40.0	12.0	40.0
50	33.120850168355595	37.0	28.0	40.0	20.0	40.0
51	33.75568504056851	36.0	31.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	57.0
9	154.0
10	208.0
11	278.0
12	341.0
13	363.0
14	445.0
15	708.0
16	1091.0
17	2141.0
18	4161.0
19	7562.0
20	13119.0
21	20642.0
22	30745.0
23	42722.0
24	56856.0
25	73470.0
26	92204.0
27	114037.0
28	139011.0
29	168512.0
30	204956.0
31	249557.0
32	310895.0
33	399050.0
34	540764.0
35	794599.0
36	1312076.0
37	2459474.0
38	3289910.0
39	5175759.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	18.032093652274913	32.61853736681657	22.76296009188778	26.586408889020742
2	27.135755684782602	26.754074424717867	25.418186789906017	20.691983100593518
3	23.482145793818727	24.540448230250867	26.084031402301193	25.893374573629213
4	21.255988860992645	27.92227717632097	24.985466794188266	25.83626716849812
5	24.659235502425854	28.791967171142286	24.60301808282928	21.945779243602583
6	25.879992544758856	25.180044718548523	26.02602111329451	22.913941623398106
7	23.95483129937243	25.969474786000397	27.665903299847088	22.409790614780082
8	24.502211098119616	25.130431249584834	26.684474976250925	23.68288267604462
9	24.51016937456589	24.814267113035257	27.33326798173853	23.342295530660323
10	22.879580442761355	25.74043249427475	28.218797139405915	23.16118992355798
11	22.66935038150068	28.05303410901807	26.1241516922301	23.153463817251144
12	22.750848549613792	25.579422502574833	28.047113770462413	23.622615177348965
13	22.93897085426358	25.949346924745274	25.591308323879925	25.52037389711122
14	23.160448268945892	28.097649470813312	25.47686134347831	23.26504091676249
15	23.07247513361069	27.820928461285956	25.412356739737916	23.694239665365437
16	23.69885082230149	25.49938184700375	25.382697004424777	25.41907032626999
17	26.322934475782393	25.118758250907558	25.43750205567311	23.120805217636935
18	23.35732209801836	25.46580746517287	25.278987892972143	25.897882543836626
19	23.676897845783564	27.683683663025683	25.24480728911051	23.394611202080245
20	25.828799029012885	25.51992245517343	25.356674601296152	23.294603914517534
21	23.6472832546642	27.55297187452236	25.327143849393813	23.47260102141963
22	25.88878276420478	25.235023897401437	25.584910746704313	23.29128259168947
23	23.42106569963504	25.52610720972122	27.759009974932074	23.29381711571166
24	23.393018256956882	27.473137592408552	25.41878011359569	23.71506403703888
25	23.661052233766956	25.346394623455502	25.474475150378534	25.518077992399007
26	23.583507407194823	26.72019693187161	26.42637272207235	23.269922938861217
27	23.714212746527604	25.23464984551012	27.649148354784348	23.401989053177928
28	25.121312122441374	25.301340718063546	26.116245009148148	23.46110215034693
29	25.150771933467773	25.312426842221686	26.17297191321738	23.363829311093156
30	25.06988643655596	25.15257770121896	26.161685864772505	23.615849997452578
31	24.59439554181739	25.53752869074744	26.147968479034066	23.720107288401103
32	24.282404018607146	25.76468137550489	26.289592264090956	23.66332234179701
33	24.222755640283324	25.497769554368766	26.359514171084886	23.919960634263024
34	24.255362646533214	25.521754019606767	26.424831370313306	23.79805196354671
35	24.075572670221018	25.670613774009453	26.410024074753625	23.843789481015897
36	24.032176201657823	25.53478779326797	26.382118513827347	24.050917491246864
37	24.472409481054594	25.525991124651505	26.26718784563523	23.734411548658667
38	24.275774271292097	25.63271199874628	26.38996715437444	23.70154657558718
39	24.219698733447398	25.5020066594135	26.350349899747645	23.927944707391458
40	24.125747217021683	25.701066757299014	26.209055022388295	23.964131003291012
41	24.157103084186826	25.731423003030464	26.34620308309047	23.765270829692238
42	24.135646693800478	25.565821201906118	26.371567670824014	23.92696443346939
43	24.151369771576828	25.679429790137544	26.30228423171354	23.866916206572093
44	24.29227124953324	25.544532489953802	26.357669708310468	23.805526552202487
45	24.213636513139864	25.351218603019372	26.33116361739135	24.103981266449416
46	24.299887719940898	25.506392095380654	26.227222335799283	23.96649784887917
47	24.20527838812011	25.535406913639804	26.30840449455593	23.950910203684153
48	21.589423876248155	35.528506301162075	23.041441724972543	19.840628097617223
49	24.260457491259764	25.670600875668377	26.116451382605426	23.952490250466436
50	24.28936267361973	25.611842482879066	26.257662420747756	23.841132422753446
51	24.34551560151091	25.386966355322212	26.19115857413999	24.076359469026894
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3906.0
1	2587.0
2	1268.0
3	1157.0
4	1046.0
5	1169.0
6	1292.0
7	1527.0
8	1762.0
9	2347.0
10	2932.0
11	4085.5
12	5239.0
13	7230.5
14	9222.0
15	12171.0
16	15120.0
17	20918.5
18	26717.0
19	35932.5
20	45148.0
21	60626.5
22	76105.0
23	148816.5
24	221528.0
25	196405.0
26	191293.0
27	211304.0
28	238546.5
29	265789.0
30	296355.5
31	326922.0
32	362892.0
33	398862.0
34	442049.0
35	485236.0
36	536386.5
37	587537.0
38	636426.5
39	685316.0
40	743031.0
41	800746.0
42	852717.5
43	904689.0
44	961042.5
45	1017396.0
46	1064539.5
47	1111683.0
48	1134392.5
49	1157102.0
50	1172711.5
51	1188321.0
52	1177998.0
53	1167675.0
54	1125694.0
55	1083713.0
56	1017182.5
57	950652.0
58	869951.5
59	789251.0
60	700728.5
61	612206.0
62	527273.5
63	442341.0
64	372745.0
65	303149.0
66	245229.5
67	187310.0
68	152284.5
69	117259.0
70	90053.5
71	62848.0
72	47347.5
73	31847.0
74	23967.5
75	12252.5
76	8417.0
77	6369.0
78	4321.0
79	3358.5
80	2396.0
81	1879.0
82	1362.0
83	1029.0
84	696.0
85	524.0
86	352.0
87	287.5
88	223.0
89	193.0
90	163.0
91	123.0
92	83.0
93	57.5
94	32.0
95	21.5
96	11.0
97	8.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.550587E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.36629690128837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.86926736198635	46.40313016158184
2	13.396941365781116	16.17447480111789
3	4.191358287789459	7.590503364611225
4	1.8511832194546836	4.469963033771372
5	0.953382049661599	2.877607193511547
6	0.5739682156867355	2.078900143202892
7	0.3973016176333069	1.6788539186590048
8	0.30477546871582517	1.4718533146183064
9	0.22428912673924783	1.2185553614824918
>10	1.1899191193378238	12.230500936122693
>50	0.03237045729999581	1.3045382042130165
>100	0.014980433251655156	1.6063061900632891
>500	1.3230489429900236E-4	0.057096334540920085
>1k	7.641069306612957E-5	0.0882206278794549
>5k	3.273664482227198E-5	0.12127219428215187
>10k+	2.182442988151465E-5	0.6282242203419087
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	82145	0.5297671140026325	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.02883875590341E-5	6.449170539931007E-6	0.0	0.0	3.224585269965503E-5
2	9.02883875590341E-5	6.449170539931007E-6	0.0	0.0	3.224585269965503E-5
3	9.02883875590341E-5	6.449170539931007E-6	0.0	0.0	5.1593364319448055E-5
4	9.02883875590341E-5	6.449170539931007E-6	0.0	0.0	7.094087593924107E-5
5	9.67375580989651E-5	6.449170539931007E-6	0.0	0.0	9.02883875590341E-5
6	9.67375580989651E-5	6.449170539931007E-6	0.0	0.0	1.2253424025868913E-4
7	1.0318672863889611E-4	1.2898341079862014E-5	0.0	0.0	1.2253424025868913E-4
8	1.0318672863889611E-4	1.2898341079862014E-5	0.0	0.0	1.3543258133855114E-4
9	1.0318672863889611E-4	1.2898341079862014E-5	0.0	6.449170539931007E-6	1.5478009295834415E-4
10	1.0318672863889611E-4	1.2898341079862014E-5	0.0	6.449170539931007E-6	1.805767751180682E-4
11	1.0963589917882712E-4	1.2898341079862014E-5	0.0	6.449170539931007E-6	1.870259456579992E-4
12	1.2253424025868913E-4	1.2898341079862014E-5	0.0	6.449170539931007E-6	2.1282262781772323E-4
13	1.2898341079862013E-4	1.2898341079862014E-5	0.0	1.2898341079862014E-5	2.1927179835765423E-4
14	1.2898341079862013E-4	1.2898341079862014E-5	0.0	1.2898341079862014E-5	2.2572096889758524E-4
15	1.3543258133855114E-4	1.2898341079862014E-5	0.0	1.2898341079862014E-5	2.4506848051737825E-4
16	1.3543258133855114E-4	1.2898341079862014E-5	0.0	1.2898341079862014E-5	2.902126742968953E-4
17	1.4833092241841315E-4	1.934751161979302E-5	0.0	1.2898341079862014E-5	3.1600935645661934E-4
18	1.6767843403820616E-4	1.934751161979302E-5	0.0	1.2898341079862014E-5	3.224585269965503E-4
19	1.805767751180682E-4	1.934751161979302E-5	0.0	2.5796682159724028E-5	3.611535502361364E-4
20	1.9992428673786122E-4	1.934751161979302E-5	0.0	2.5796682159724028E-5	3.740518913159984E-4
21	2.2572096889758524E-4	2.5796682159724028E-5	0.0	2.5796682159724028E-5	3.933994029357914E-4
22	2.515176510573093E-4	2.5796682159724028E-5	0.0	2.5796682159724028E-5	4.191960850955154E-4
23	2.5796682159724026E-4	2.5796682159724028E-5	0.0	2.5796682159724028E-5	4.4499276725523944E-4
24	2.708651626771023E-4	2.5796682159724028E-5	0.0	2.5796682159724028E-5	4.578911083351015E-4
25	3.095601859166883E-4	2.5796682159724028E-5	0.0	2.5796682159724028E-5	5.030353021146186E-4
26	3.676027207760674E-4	3.224585269965503E-5	0.0	3.224585269965503E-5	5.159336431944805E-4
27	3.933994029357914E-4	3.224585269965503E-5	0.0	3.869502323958604E-5	5.223828137344115E-4
28	4.1274691455558444E-4	3.869502323958604E-5	0.0	5.1593364319448055E-5	5.352811548142736E-4
29	4.514419377951705E-4	3.869502323958604E-5	0.0	5.804253485937906E-5	5.417303253542045E-4
30	5.030353021146186E-4	4.514419377951705E-5	0.0	5.804253485937906E-5	5.610778369739976E-4
31	5.868745191337216E-4	4.514419377951705E-5	0.0	5.804253485937906E-5	5.675270075139286E-4
32	6.578153950729627E-4	4.514419377951705E-5	0.0	6.449170539931007E-5	6.062220307535147E-4
33	7.416546120920657E-4	4.514419377951705E-5	0.0	6.449170539931007E-5	6.320187129132387E-4
34	7.932479764115138E-4	4.514419377951705E-5	0.0	6.449170539931007E-5	6.384678834531697E-4
35	8.577396818108239E-4	4.514419377951705E-5	0.0	7.094087593924107E-5	7.029595888524798E-4
36	0.001012519774769168	4.514419377951705E-5	0.0	7.094087593924107E-5	7.287562710122038E-4
37	0.001083460650708409	4.514419377951705E-5	0.0	8.383921701910308E-5	7.416546120920657E-4
38	0.0011737490382674432	5.804253485937906E-5	0.0	8.383921701910308E-5	7.481037826319968E-4
39	0.0012317915731268224	5.804253485937906E-5	0.0	9.67375580989651E-5	7.610021237118588E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	42260	0.0	37.69522	1
TCAACGC	43425	0.0	36.45596	12
CAACGCA	43470	0.0	36.35611	13
AACGCAG	44045	0.0	35.86105	14
ATCAACG	44270	0.0	35.74486	11
ACGCAGA	44295	0.0	35.612938	15
GGTATCA	44705	0.0	35.543003	8
GTGGTAT	44535	0.0	35.51701	6
AGTGGTA	44610	0.0	35.401814	5
GTATCAA	45020	0.0	35.39427	9
TGGTATC	45240	0.0	35.013264	7
TATCAAC	45470	0.0	34.88069	10
CGCAGAG	45315	0.0	34.75174	16
AGAGTAC	45610	0.0	34.04352	19
CAGAGTA	47695	0.0	32.956287	18
GCAGTGG	48330	0.0	32.877094	3
AGCAGTG	48415	0.0	32.870495	2
CAGTGGT	48310	0.0	32.81619	4
GCAGAGT	48480	0.0	32.52475	17
GTACATG	31285	0.0	31.565445	22
>>END_MODULE