FastQCFastQC Report
Thu 26 May 2016
SRR805441_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805441_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24390555
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5968272.4469594890317174No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT432330.1772530391374858No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG265610.10889871099694123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2544400.043.1323741
TCAACGC2555400.042.9176512
CAACGCA2557200.042.80032713
ATCAACG2566000.042.7456211
AACGCAG2567150.042.60902414
ACGCAGA2570800.042.55640815
TATCAAC2582850.042.43103410
GTATCAA2209250.042.3795439
GGTATCA2214050.042.3527038
CGCAGAG2582400.042.34694716
GTGGTAT2215600.042.312926
AGTGGTA2219450.042.2283675
TGGTATC2222500.042.1997767
AGAGTAC2558650.042.1728319
AGCAGTG2622650.041.855762
CAGAGTA2592000.041.77951418
GCAGAGT2614650.041.63014217
CAGTGGT2266050.041.3808174
GCAGTGG2269600.041.3458333
GTACATG1585900.041.1722122