##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805441_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24390555
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.24694345823619	37.0	35.0	37.0	35.0	37.0
2	36.22856544264778	37.0	36.0	37.0	35.0	37.0
3	37.96818817775979	39.0	38.0	39.0	35.0	39.0
4	37.8999298703945	39.0	38.0	39.0	35.0	39.0
5	37.77543561431874	39.0	38.0	39.0	35.0	39.0
6	37.77084301689732	39.0	38.0	39.0	35.0	39.0
7	37.71225824914603	39.0	37.0	39.0	35.0	39.0
8	38.75935246245934	40.0	39.0	40.0	36.0	40.0
9	33.1354344335338	40.0	36.0	40.0	3.0	40.0
10	34.98683391993335	40.0	36.0	40.0	17.0	40.0
11	37.2417229948232	40.0	36.0	40.0	32.0	40.0
12	37.99138773184948	40.0	37.0	40.0	34.0	40.0
13	38.35807085980618	40.0	38.0	40.0	35.0	40.0
14	38.42985315422302	40.0	38.0	40.0	35.0	40.0
15	38.35640468205828	40.0	38.0	40.0	35.0	40.0
16	38.36558594095132	40.0	38.0	40.0	35.0	40.0
17	38.227821384138245	40.0	38.0	40.0	35.0	40.0
18	38.208860683981975	40.0	38.0	40.0	34.0	40.0
19	38.041730620725936	40.0	38.0	40.0	34.0	40.0
20	37.90061882560688	40.0	38.0	40.0	34.0	40.0
21	37.7131503567672	40.0	37.0	40.0	34.0	40.0
22	37.637381355200816	39.0	37.0	40.0	34.0	40.0
23	37.32688796954395	39.0	37.0	40.0	33.0	40.0
24	37.304068685603916	39.0	37.0	40.0	33.0	40.0
25	37.16198872883376	38.0	36.0	40.0	33.0	40.0
26	36.995339097449815	38.0	36.0	40.0	32.0	40.0
27	36.939724372815625	38.0	36.0	40.0	32.0	40.0
28	36.857369297254614	38.0	36.0	40.0	32.0	40.0
29	36.65812832057327	38.0	36.0	40.0	32.0	40.0
30	36.43135221810246	38.0	36.0	40.0	31.0	40.0
31	36.20020827734342	38.0	35.0	40.0	31.0	40.0
32	35.97050571419962	38.0	35.0	39.0	30.0	40.0
33	35.716484393241565	38.0	35.0	39.0	30.0	40.0
34	35.45324827581824	38.0	34.0	39.0	30.0	40.0
35	35.27496910176911	37.0	34.0	39.0	30.0	40.0
36	35.05566285802025	37.0	34.0	38.0	29.0	40.0
37	34.89054017016013	37.0	34.0	38.0	29.0	40.0
38	34.59462254958938	36.0	33.0	38.0	28.0	40.0
39	34.48408652447638	36.0	33.0	38.0	28.0	39.0
40	34.31713915489008	36.0	33.0	38.0	28.0	39.0
41	34.05318009368791	36.0	33.0	38.0	27.0	39.0
42	33.70630430508859	35.0	32.0	38.0	26.0	39.0
43	33.55376001079106	35.0	32.0	38.0	26.0	39.0
44	33.21572711240068	35.0	31.0	38.0	26.0	39.0
45	32.949063561694274	35.0	31.0	38.0	24.0	39.0
46	32.753420863116894	34.0	31.0	37.0	24.0	38.0
47	32.506700114040044	34.0	31.0	37.0	24.0	38.0
48	32.16118764005165	34.0	30.0	37.0	24.0	38.0
49	31.78164781408213	34.0	30.0	36.0	23.0	38.0
50	31.34521772874787	33.0	29.0	36.0	22.0	38.0
51	33.77391158175777	36.0	32.0	38.0	26.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	38.0
8	189.0
9	345.0
10	453.0
11	493.0
12	521.0
13	559.0
14	586.0
15	741.0
16	1336.0
17	2571.0
18	5079.0
19	10123.0
20	18670.0
21	31586.0
22	49623.0
23	72331.0
24	100196.0
25	134596.0
26	174438.0
27	220779.0
28	274937.0
29	339212.0
30	417724.0
31	521282.0
32	673640.0
33	920269.0
34	1381384.0
35	2404641.0
36	4642236.0
37	7144439.0
38	4772744.0
39	72794.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.568397890084912	36.782164243495075	29.288825121035583	21.360612745384433
2	26.257266388567214	42.69775739010449	13.096344056131565	17.94863216519673
3	34.84819431128156	25.952664873759534	18.46461468383971	20.734526131119196
4	21.950988815137663	23.30326636683749	20.367798108735123	34.37794670928972
5	20.307114782750947	33.98314634496837	23.734502966414663	21.97523590586602
6	31.701496747408985	23.658116840719696	21.983165204727815	22.6572212071435
7	22.323682261432758	22.91543591361492	32.960381590332815	21.80050023461951
8	31.167035764458824	24.910212170243767	23.029541558197426	20.893210507099983
9	27.642331222065263	33.93126150675948	20.67088264289189	17.755524628283368
10	21.03680297557805	24.54592361674427	33.291653264962605	21.12562014271508
11	20.913374049913994	34.44806401494349	23.515233663194625	21.12332827194789
12	20.763939155956066	24.253052052321074	33.653887744661816	21.329121047061044
13	20.753221892654757	24.41783305053944	23.788454178266957	31.040490878538847
14	21.222255910125867	34.54005044165662	23.338923612029326	20.89877003618819
15	21.08545295504756	34.148948230165324	23.46960944513153	21.29598936965559
16	21.2146669069236	23.959696694068665	23.688013659385774	31.137622739621957
17	31.273093211696086	23.95186169400409	23.59000850944146	21.18503658485836
18	21.105813295351417	24.08996433250494	23.60140226411412	31.20282010802952
19	21.430758750672137	33.88069275176395	23.6731144494252	21.015434048138715
20	31.369851977538026	24.16197581399849	23.55851271117037	20.909659497293113
21	21.31493112805346	33.95990374142778	23.59432985432271	21.130835276196052
22	31.137688338785242	23.792160530992426	23.99591973204382	21.074231398178515
23	21.26445667185515	24.112370546713677	33.52614157406422	21.09703120736695
24	21.26737583462123	33.7029887183789	23.605137316473527	21.424498130526345
25	21.071873928248046	24.001307063328404	23.81491934070381	31.11189966771974
26	21.636350628347735	29.92542810116457	27.488730781238885	20.94949048924881
27	21.50908825157935	23.861937540986663	33.4685209090158	21.160453298418176
28	27.384981604559634	23.878415230813733	27.524855420469112	21.21174774415752
29	27.525113717174538	24.033311255114942	27.296697430624274	21.144877597086246
30	27.41913826889138	23.92443714380423	27.40265647911661	21.253768108187778
31	25.01815969337311	24.90776040151608	28.076060589847174	21.998019315263633
32	23.844131468103125	25.799142331939557	28.220108152520513	22.136618047436805
33	23.613833305556188	25.225789245058177	28.615154513704177	22.545222935681455
34	22.99908304669574	25.118518213300188	28.9852567930496	22.897141946954466
35	22.85419499474284	25.324884981092065	28.9664462329783	22.854473791186795
36	23.187381344950943	25.187393234799288	29.072901375142962	22.552324045106804
37	23.26873250731687	25.13840295967025	29.043324352397885	22.549540180614997
38	23.506918969248545	25.295033261850747	28.863939340453715	22.334108428446996
39	23.34860358856123	25.20417432075654	28.85706372815215	22.590158362530087
40	22.952552740189798	25.554764948973073	28.763105226592835	22.72957708424429
41	23.309125192108173	25.75089824729286	28.53988357378502	22.400092986813952
42	23.328440045747218	25.676029102248798	28.644694636919905	22.350836215084076
43	23.028032777441922	25.44783831282232	29.159939164975953	22.364189744759805
44	23.50588988237455	25.283664106864318	28.688551777522076	22.521894233239053
45	23.349111982076668	25.035125276977094	28.821742678672134	22.794020062274107
46	23.0425342924751	25.124528736635966	28.69010975765004	23.142827213238895
47	23.12952288293563	25.28565258150132	28.656834582074907	22.927989953488144
48	23.07329210015926	25.194920738785974	28.691298742484538	23.04048841857022
49	22.808824973437464	25.638838476615234	28.62915993506503	22.923176614882276
50	23.409254114963762	25.573309012443545	28.29641227926138	22.72102459333131
51	23.193506666822465	25.531341947733456	28.491799387098816	22.78335199834526
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10820.0
1	6518.0
2	2216.0
3	1753.0
4	1290.0
5	1289.0
6	1288.0
7	1517.5
8	1747.0
9	2317.0
10	2887.0
11	4283.0
12	5679.0
13	8188.0
14	10697.0
15	15006.0
16	19315.0
17	28394.5
18	37474.0
19	51553.5
20	65633.0
21	143580.0
22	221527.0
23	526349.5
24	831172.0
25	589478.0
26	356289.5
27	364795.0
28	403659.0
29	442523.0
30	487663.5
31	532804.0
32	590502.5
33	648201.0
34	721645.0
35	795089.0
36	886539.5
37	977990.0
38	1057088.5
39	1136187.0
40	1219556.5
41	1302926.0
42	1376022.5
43	1449119.0
44	1525710.5
45	1602302.0
46	1663216.0
47	1724130.0
48	1750246.5
49	1776363.0
50	1779392.0
51	1782421.0
52	1762957.5
53	1743494.0
54	1699083.5
55	1654673.0
56	1531039.5
57	1407406.0
58	1263728.5
59	1120051.0
60	982452.5
61	844854.0
62	722535.0
63	600216.0
64	497912.0
65	395608.0
66	316769.0
67	237930.0
68	190425.5
69	142921.0
70	108885.0
71	74849.0
72	55652.0
73	36455.0
74	27552.5
75	14133.5
76	9617.0
77	7065.0
78	4513.0
79	3523.0
80	2533.0
81	1919.5
82	1306.0
83	973.5
84	641.0
85	447.5
86	254.0
87	187.5
88	121.0
89	89.5
90	58.0
91	38.0
92	18.0
93	11.5
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.4390555E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.38419670414774
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.37374669125244	35.74119342816972
2	15.34960456482389	15.160557823202941
3	5.135902679008483	7.608972844605429
4	2.2440819832811743	4.43288744330366
5	1.212771883635676	2.9945882629361997
6	0.7397342344318057	2.1918708565183462
7	0.4765689737599143	1.6474483160277404
8	0.36822224420321203	1.454748779085932
9	0.2535011169346267	1.1267054121078686
>10	1.6832020583082294	15.117553592840395
>50	0.10347220314247091	3.5346819368650557
>100	0.05761153201752899	4.838189929006532
>500	9.63216844920275E-4	0.31688022706405755
>1k	4.952152348097462E-4	0.5213550101609974
>5k	6.070156042018735E-5	0.21715751106020417
>10k+	6.070156042010586E-5	3.0952086270449337
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	596827	2.4469594890317174	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT	43233	0.1772530391374858	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAG	26561	0.10889871099694123	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.559916328267233E-5	0.0	0.0	0.0	4.0999477051670206E-6
2	7.379905869300638E-5	0.0	0.0	0.0	1.2299843115501062E-5
3	7.379905869300638E-5	0.0	0.0	8.199895410334041E-6	2.0499738525835103E-5
4	7.379905869300638E-5	0.0	0.0	8.199895410334041E-6	2.0499738525835103E-5
5	7.78990063981734E-5	0.0	0.0	1.2299843115501062E-5	2.4599686231002123E-5
6	8.199895410334041E-5	0.0	0.0	1.6399790820668082E-5	2.4599686231002123E-5
7	9.019884951367446E-5	0.0	0.0	2.4599686231002123E-5	2.4599686231002123E-5
8	9.429879721884148E-5	0.0	0.0	2.8699633936169144E-5	2.8699633936169144E-5
9	1.0659864033434254E-4	0.0	0.0	3.2799581641336164E-5	3.689952934650319E-5
10	1.0659864033434254E-4	4.0999477051670206E-6	0.0	3.2799581641336164E-5	4.509942475683723E-5
11	1.0659864033434254E-4	1.6399790820668082E-5	0.0	3.689952934650319E-5	5.739926787233829E-5
12	1.1069858803950956E-4	1.6399790820668082E-5	0.0	4.509942475683723E-5	6.149921557750531E-5
13	1.1479853574467658E-4	1.6399790820668082E-5	0.0	4.509942475683723E-5	6.969911098783935E-5
14	1.2299843115501062E-4	1.6399790820668082E-5	0.0	5.329932016717127E-5	7.78990063981734E-5
15	1.3119832656534466E-4	1.6399790820668082E-5	0.0	5.739926787233829E-5	8.609890180850743E-5
16	1.352982742705117E-4	2.0499738525835103E-5	0.0	5.739926787233829E-5	1.0249869262917551E-4
17	1.393982219756787E-4	2.0499738525835103E-5	0.0	5.739926787233829E-5	1.393982219756787E-4
18	1.6809785591184785E-4	2.0499738525835103E-5	0.0	5.739926787233829E-5	1.4759811738601275E-4
19	1.8449764673251592E-4	2.0499738525835103E-5	0.0	6.559916328267233E-5	1.557980127963468E-4
20	2.0909733296351805E-4	2.0499738525835103E-5	0.0	6.559916328267233E-5	1.6809785591184785E-4
21	2.4599686231002125E-4	2.0499738525835103E-5	0.0	6.559916328267233E-5	1.762977513221819E-4
22	2.6649660083585635E-4	2.4599686231002123E-5	0.0	7.379905869300638E-5	1.96797489848017E-4
23	2.787964439513574E-4	2.4599686231002123E-5	0.0	7.379905869300638E-5	2.0909733296351805E-4
24	3.2799581641336164E-4	2.4599686231002123E-5	0.0	7.78990063981734E-5	2.2139717607901912E-4
25	3.6899529346503184E-4	2.4599686231002123E-5	0.0	8.609890180850743E-5	2.2959707148935315E-4
26	4.0999477051670204E-4	2.4599686231002123E-5	0.0	8.609890180850743E-5	2.4189691460485422E-4
27	4.3049450904253714E-4	2.4599686231002123E-5	0.0	9.019884951367446E-5	2.4599686231002125E-4
28	4.6739403838904036E-4	2.8699633936169144E-5	0.0	9.429879721884148E-5	2.5419675772035525E-4
29	5.206933585562117E-4	2.8699633936169144E-5	0.0	1.0659864033434254E-4	2.5419675772035525E-4
30	6.02692312659552E-4	3.2799581641336164E-5	0.0	1.1069858803950956E-4	2.5419675772035525E-4
31	7.379905869300637E-4	4.0999477051670206E-5	0.0	1.1479853574467658E-4	2.582967054255223E-4
32	9.306881290729136E-4	4.919937246200425E-5	0.0	1.1479853574467658E-4	2.6649660083585635E-4
33	0.0010454866648175903	4.919937246200425E-5	4.0999477051670206E-6	1.1889848344984359E-4	2.705965485410234E-4
34	0.0011274856189209307	4.919937246200425E-5	4.0999477051670206E-6	1.2299843115501062E-4	2.705965485410234E-4
35	0.0012012846776139371	4.919937246200425E-5	4.0999477051670206E-6	1.2709837886017763E-4	2.787964439513574E-4
36	0.0012955834748327785	5.329932016717127E-5	4.0999477051670206E-6	1.2709837886017763E-4	2.828963916565244E-4
37	0.0014390816445136242	5.329932016717127E-5	4.0999477051670206E-6	1.2709837886017763E-4	2.8699633936169145E-4
38	0.0015825798141944698	5.329932016717127E-5	4.0999477051670206E-6	1.2709837886017763E-4	2.910962870668585E-4
39	0.0017014782976443135	5.329932016717127E-5	4.0999477051670206E-6	1.3119832656534466E-4	2.910962870668585E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	254440	0.0	43.132374	1
TCAACGC	255540	0.0	42.91765	12
CAACGCA	255720	0.0	42.800327	13
ATCAACG	256600	0.0	42.74562	11
AACGCAG	256715	0.0	42.609024	14
ACGCAGA	257080	0.0	42.556408	15
TATCAAC	258285	0.0	42.431034	10
GTATCAA	220925	0.0	42.379543	9
GGTATCA	221405	0.0	42.352703	8
CGCAGAG	258240	0.0	42.346947	16
GTGGTAT	221560	0.0	42.31292	6
AGTGGTA	221945	0.0	42.228367	5
TGGTATC	222250	0.0	42.199776	7
AGAGTAC	255865	0.0	42.17283	19
AGCAGTG	262265	0.0	41.85576	2
CAGAGTA	259200	0.0	41.779514	18
GCAGAGT	261465	0.0	41.630142	17
CAGTGGT	226605	0.0	41.380817	4
GCAGTGG	226960	0.0	41.345833	3
GTACATG	158590	0.0	41.17221	22
>>END_MODULE