FastQCFastQC Report
Thu 26 May 2016
SRR805440_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805440_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10430831
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1675001.6058164493317935No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC135840.13022931729983928No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT120660.11567630613514877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC660100.041.29828612
AAGCAGT655050.041.2594451
CAACGCA663750.041.2474613
ATCAACG665550.040.9634911
GTGGTAT661950.040.934746
GTATCAA665700.040.903569
ACGCAGA668650.040.86106515
GGTATCA666700.040.845588
AACGCAG670100.040.83308414
AGTGGTA666650.040.774395
TGGTATC670800.040.61277
TATCAAC671400.040.556310
CGCAGAG675900.040.43608516
AGAGTAC678500.039.9064119
CAGTGGT687150.039.620174
AGCAGTG690950.039.54232
CAGAGTA688850.039.43746618
GCAGTGG693400.039.3441733
GCAGAGT697000.039.16678217
GTACATG431900.038.7850222
Produced by FastQC (version 0.11.5)