##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805440_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10430831
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.47843292638909	35.0	35.0	35.0	33.0	37.0
2	35.4159335914847	37.0	35.0	37.0	35.0	37.0
3	37.78481839078785	39.0	38.0	39.0	35.0	39.0
4	37.73736665851455	39.0	38.0	39.0	35.0	39.0
5	37.768442706050934	39.0	38.0	39.0	35.0	39.0
6	37.52675688063588	39.0	38.0	39.0	35.0	39.0
7	37.646763234875536	39.0	37.0	39.0	35.0	39.0
8	38.68562389707972	40.0	39.0	40.0	36.0	40.0
9	38.67716800320128	40.0	39.0	40.0	36.0	40.0
10	38.59785716018216	40.0	38.0	40.0	36.0	40.0
11	38.50916106300639	40.0	38.0	40.0	36.0	40.0
12	38.396276864230664	40.0	38.0	40.0	36.0	40.0
13	38.48447549385087	40.0	38.0	40.0	36.0	40.0
14	38.374204701427914	40.0	38.0	40.0	35.0	40.0
15	38.32974870362678	40.0	38.0	40.0	35.0	40.0
16	38.23893187417187	40.0	38.0	40.0	35.0	40.0
17	38.0618885494358	40.0	38.0	40.0	34.0	40.0
18	37.956850705375246	40.0	38.0	40.0	34.0	40.0
19	37.8465755029489	40.0	38.0	40.0	34.0	40.0
20	37.58586089641372	39.0	37.0	40.0	34.0	40.0
21	37.32647638524678	39.0	37.0	40.0	33.0	40.0
22	37.30058870669077	39.0	37.0	40.0	33.0	40.0
23	37.051993364670565	38.0	36.0	40.0	33.0	40.0
24	36.82240897201766	38.0	36.0	40.0	32.0	40.0
25	36.58047733684881	38.0	36.0	40.0	31.0	40.0
26	36.46866476889521	38.0	36.0	40.0	31.0	40.0
27	36.22846904527549	38.0	35.0	40.0	31.0	40.0
28	36.01515785271567	38.0	35.0	39.0	30.0	40.0
29	35.57557878178642	38.0	34.0	39.0	30.0	40.0
30	35.40620656206586	38.0	34.0	39.0	30.0	40.0
31	35.15015246628001	37.0	34.0	39.0	29.0	40.0
32	34.91041845084059	37.0	33.0	38.0	29.0	40.0
33	34.63984317261012	37.0	33.0	38.0	28.0	40.0
34	34.369731711692005	36.0	33.0	38.0	27.0	40.0
35	34.03411022573369	36.0	32.0	38.0	27.0	39.0
36	33.9184018991392	36.0	32.0	38.0	27.0	39.0
37	33.751004881586134	35.0	32.0	38.0	27.0	39.0
38	33.48520448658405	35.0	31.0	38.0	26.0	39.0
39	33.22791443941523	35.0	31.0	38.0	25.0	39.0
40	32.9861715715651	35.0	31.0	38.0	25.0	39.0
41	32.70047573390845	34.0	31.0	37.0	24.0	39.0
42	32.34899903948209	34.0	30.0	37.0	24.0	38.0
43	32.04620216740162	34.0	30.0	37.0	24.0	38.0
44	31.59277002954031	33.0	29.0	37.0	23.0	38.0
45	31.168147868563874	33.0	29.0	36.0	22.0	38.0
46	30.866715413182323	33.0	28.0	36.0	22.0	38.0
47	30.50532273027911	32.0	28.0	36.0	21.0	38.0
48	30.05008757212153	32.0	27.0	35.0	19.0	38.0
49	29.549180501534345	31.0	26.0	35.0	18.0	37.0
50	29.35419191433549	31.0	26.0	35.0	18.0	37.0
51	31.009350261738494	33.0	29.0	36.0	22.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	17.0
8	111.0
9	222.0
10	298.0
11	339.0
12	421.0
13	541.0
14	785.0
15	1207.0
16	1944.0
17	3378.0
18	5319.0
19	8798.0
20	14193.0
21	21307.0
22	30591.0
23	42268.0
24	56438.0
25	72281.0
26	90490.0
27	111542.0
28	135882.0
29	164399.0
30	201228.0
31	250644.0
32	327249.0
33	461712.0
34	743734.0
35	1488967.0
36	2945581.0
37	2745770.0
38	500031.0
39	3144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.432869634260204	44.971134131115726	29.570788751155106	15.025207483468957
2	31.505706496443093	42.75064949283523	13.820662994156457	11.922981016565219
3	36.81614628786527	25.18837664995243	20.62658286765455	17.368894194527744
4	23.57592602161803	26.345475255039602	19.756997309226847	30.321601414115516
5	19.835965130678467	33.67831383712382	24.517260417698264	21.968460614499456
6	28.552873687628534	24.115442000738003	22.811250608892042	24.52043370274142
7	23.243804832040706	24.131653556653347	29.706683964106023	22.91785764719992
8	26.302842026680327	25.632051751197967	25.489321033003026	22.57578518911868
9	28.003991244801107	24.543269850695502	25.063794054375915	22.388944850127473
10	22.40020953268249	25.812718085452634	29.86989243714139	21.917179944723483
11	21.829027811878078	31.328587338822768	24.968988568600142	21.873396280699016
12	22.18338117068525	25.113761310100795	30.495451417053925	22.20740610216003
13	22.021505285628727	25.722178798601952	24.69356468338908	27.56275123238024
14	22.272022238688365	31.390346560115873	24.441437120398174	21.89619408079759
15	22.237825538540505	30.892466765112005	24.31791867781196	22.55178901853553
16	22.50525389587848	25.133836412458415	24.526531011766945	27.834378679896165
17	28.110147695806788	24.87514177921203	24.684505002525686	22.330205522455497
18	22.4284431412991	24.793825151610644	24.465193616884406	28.31253809020585
19	22.494995844530507	30.897749182208013	24.451570541215748	22.15568443204573
20	28.354097578610947	25.21164421128096	24.50350312453533	21.93075508557276
21	22.839867696063717	30.524241069575375	24.30124694763054	22.334644286730367
22	27.971069610848836	25.199171571277496	24.681734369965348	22.14802444790832
23	22.43818349659773	25.06505953360763	30.475807728070752	22.020949241723887
24	22.721152322379684	30.249996380921136	24.398813479002776	22.630037817696405
25	22.301051565306736	25.24567793304292	24.42644310889516	28.026827392755187
26	22.526345216407016	28.443534364615818	26.788316290427865	22.241804128549298
27	22.8461855052584	24.5849923174865	30.05410594803041	22.51471622922469
28	26.111035640401038	24.907507369259456	26.67031993903458	22.311137051304925
29	26.360335049048345	24.839238599494134	26.659822213589695	22.140604137867825
30	26.356270176364667	24.622678672485442	26.498751633498806	22.52229951765109
31	24.996512741889884	25.410698342250964	26.870975092971978	22.721813822887167
32	24.156502967021513	25.939994617878483	27.133600381407767	22.769902033692237
33	24.097351399902845	25.40141816121841	27.108961884244888	23.392268554633855
34	23.549494762210223	25.70708891746017	27.369113736000518	23.37430258432909
35	23.475550509829944	25.752972126573614	27.552694507273678	23.21878285632276
36	23.796857604154454	25.370989137874055	27.472614598012374	23.359538659959114
37	23.744877086015485	25.65569320411768	27.439031463552617	23.16039824631422
38	23.797730017867224	25.535568546743782	27.66188043886436	23.00482099652463
39	24.068590508273022	25.306219609923698	27.388086337512323	23.237103544290957
40	23.51081136296811	25.853817399591655	27.274001467380693	23.361369770059547
41	23.65324488528287	25.975418449402543	27.33798486429317	23.033351801021414
42	23.868165441468662	25.57804838368103	27.235615264018755	23.318170910831554
43	23.62025614258346	25.91175142229799	27.520415199901137	22.947577235217405
44	23.795572950994988	25.578000448861648	27.437545484151745	23.18888111599162
45	24.01506648894992	25.16911643952433	27.25579582297901	23.560021248546736
46	23.633188956853008	25.52910693309095	27.245183053967608	23.592521056088437
47	23.66878535372685	25.51394994320203	27.335540188504638	23.481724514566483
48	23.86240367617882	25.073131757191735	27.32973048839541	23.734734078234034
49	23.632230260465345	25.778559733160282	27.194103710433044	23.395106295941332
50	23.759286292722027	25.639337843744187	27.334303470164556	23.267072393369233
51	23.879669798120588	25.29971964841536	27.22173334032543	23.59887721313863
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6210.0
1	3905.0
2	1600.0
3	1377.0
4	1154.0
5	1184.0
6	1214.0
7	1288.0
8	1362.0
9	1710.5
10	2059.0
11	2936.5
12	3814.0
13	5383.0
14	6952.0
15	9118.0
16	11284.0
17	15856.5
18	20429.0
19	27071.5
20	33714.0
21	44529.5
22	55345.0
23	160727.5
24	266110.0
25	203556.5
26	147141.5
27	153280.0
28	169211.0
29	185142.0
30	204359.0
31	223576.0
32	247028.5
33	270481.0
34	300602.5
35	330724.0
36	364163.0
37	397602.0
38	431331.5
39	465061.0
40	502724.5
41	540388.0
42	574789.0
43	609190.0
44	647654.0
45	686118.0
46	722643.0
47	759168.0
48	768464.5
49	777761.0
50	787398.0
51	797035.0
52	791840.0
53	786645.0
54	754433.0
55	722221.0
56	671804.5
57	621388.0
58	560535.5
59	499683.0
60	437526.0
61	375369.0
62	321401.5
63	267434.0
64	222439.0
65	177444.0
66	142035.5
67	106627.0
68	85606.5
69	64586.0
70	48123.5
71	31661.0
72	23308.5
73	14956.0
74	11207.5
75	5556.0
76	3653.0
77	2674.0
78	1695.0
79	1355.5
80	1016.0
81	863.0
82	710.0
83	505.0
84	300.0
85	203.0
86	106.0
87	80.0
88	54.0
89	37.5
90	21.0
91	16.0
92	11.0
93	8.5
94	6.0
95	7.0
96	8.0
97	5.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.0430831E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.015630773284336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.92539414614632	45.3980065801834
2	13.367453468949595	15.777773966051761
3	4.185893025875843	7.41099351814664
4	1.8661375477790683	4.40525137967567
5	1.0115199226482203	2.984774313741425
6	0.6189488811106297	2.191659518109743
7	0.42532907516091845	1.7570744559787486
8	0.2844289511379465	1.3428603169271662
9	0.2210894284677197	1.1742958870494664
>10	1.0228266036818188	10.310805833315925
>50	0.04765400481820861	1.9017916284955725
>100	0.02175030743599209	2.3323783393675357
>500	0.0010972912760922229	0.4279975662036536
>1k	3.8894837194474417E-4	0.4074962875849498
>5k	1.7679427931495376E-5	0.056466783429683705
>10k+	7.07177117259815E-5	2.120373625738631
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	167500	1.6058164493317935	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC	13584	0.13022931729983928	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGT	12066	0.11567630613514877	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.8347855506430885E-5	1.9173927753215443E-5	0.0	0.0	0.0
2	2.0132624140876217E-4	1.9173927753215443E-5	0.0	0.0	0.0
3	2.0132624140876217E-4	1.9173927753215443E-5	0.0	0.0	9.586963876607721E-6
4	2.0132624140876217E-4	1.9173927753215443E-5	0.0	0.0	9.586963876607721E-6
5	2.0132624140876217E-4	1.9173927753215443E-5	0.0	0.0	9.586963876607721E-6
6	2.1091320528536988E-4	1.9173927753215443E-5	0.0	0.0	9.586963876607721E-6
7	2.1091320528536988E-4	1.9173927753215443E-5	0.0	0.0	9.586963876607721E-6
8	2.3008713303858532E-4	1.9173927753215443E-5	0.0	0.0	4.793481938303861E-5
9	2.3008713303858532E-4	1.9173927753215443E-5	0.0	0.0	7.669571101286177E-5
10	2.3008713303858532E-4	1.9173927753215443E-5	0.0	0.0	7.669571101286177E-5
11	2.3008713303858532E-4	1.9173927753215443E-5	0.0	0.0	7.669571101286177E-5
12	2.3008713303858532E-4	1.9173927753215443E-5	0.0	9.586963876607721E-6	7.669571101286177E-5
13	2.3008713303858532E-4	1.9173927753215443E-5	0.0	9.586963876607721E-6	7.669571101286177E-5
14	2.3008713303858532E-4	1.9173927753215443E-5	0.0	9.586963876607721E-6	9.586963876607722E-5
15	2.3967409691519304E-4	1.9173927753215443E-5	0.0	9.586963876607721E-6	9.586963876607722E-5
16	2.4926106079180076E-4	1.9173927753215443E-5	0.0	9.586963876607721E-6	1.0545660264268494E-4
17	2.684349885450162E-4	1.9173927753215443E-5	0.0	1.9173927753215443E-5	1.2463053039590038E-4
18	2.684349885450162E-4	1.9173927753215443E-5	0.0	1.9173927753215443E-5	1.2463053039590038E-4
19	2.780219524216239E-4	1.9173927753215443E-5	0.0	1.9173927753215443E-5	1.342174942725081E-4
20	2.780219524216239E-4	1.9173927753215443E-5	0.0	1.9173927753215443E-5	1.342174942725081E-4
21	2.8760891629823164E-4	1.9173927753215443E-5	0.0	1.9173927753215443E-5	1.4380445814911582E-4
22	3.163698079280548E-4	1.9173927753215443E-5	0.0	1.9173927753215443E-5	1.4380445814911582E-4
23	3.3554373568127024E-4	1.9173927753215443E-5	0.0	1.9173927753215443E-5	1.72565349778939E-4
24	3.5471766343448573E-4	1.9173927753215443E-5	0.0	1.9173927753215443E-5	1.72565349778939E-4
25	4.1223944669413205E-4	1.9173927753215443E-5	0.0	1.9173927753215443E-5	1.72565349778939E-4
26	4.5058730220056293E-4	1.9173927753215443E-5	0.0	1.9173927753215443E-5	1.9173927753215445E-4
27	4.793481938303861E-4	1.9173927753215443E-5	0.0	1.9173927753215443E-5	2.0132624140876217E-4
28	5.081090854602093E-4	1.9173927753215443E-5	0.0	2.8760891629823165E-5	2.1091320528536988E-4
29	5.560439048432478E-4	1.9173927753215443E-5	0.0	2.8760891629823165E-5	2.3967409691519304E-4
30	6.231526519795019E-4	1.9173927753215443E-5	0.0	2.8760891629823165E-5	2.588480246684085E-4
31	6.51913543609325E-4	1.9173927753215443E-5	0.0	2.8760891629823165E-5	2.588480246684085E-4
32	7.190222907455791E-4	1.9173927753215443E-5	0.0	3.8347855506430885E-5	2.684349885450162E-4
33	8.436528211414795E-4	1.9173927753215443E-5	0.0	3.8347855506430885E-5	2.684349885450162E-4
34	9.203485321543413E-4	1.9173927753215443E-5	0.0	5.752178325964633E-5	2.684349885450162E-4
35	9.491094237841645E-4	1.9173927753215443E-5	0.0	5.752178325964633E-5	2.8760891629823164E-4
36	0.0010066312070438107	1.9173927753215443E-5	0.0	5.752178325964633E-5	3.067828440514471E-4
37	0.0011312617374397112	1.9173927753215443E-5	0.0	5.752178325964633E-5	3.45130699557878E-4
38	0.0012558922678356115	1.9173927753215443E-5	0.0	6.710874713625405E-5	3.45130699557878E-4
39	0.001342174942725081	1.9173927753215443E-5	0.0	6.710874713625405E-5	3.5471766343448573E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	66010	0.0	41.298286	12
AAGCAGT	65505	0.0	41.259445	1
CAACGCA	66375	0.0	41.24746	13
ATCAACG	66555	0.0	40.96349	11
GTGGTAT	66195	0.0	40.93474	6
GTATCAA	66570	0.0	40.90356	9
ACGCAGA	66865	0.0	40.861065	15
GGTATCA	66670	0.0	40.84558	8
AACGCAG	67010	0.0	40.833084	14
AGTGGTA	66665	0.0	40.77439	5
TGGTATC	67080	0.0	40.6127	7
TATCAAC	67140	0.0	40.5563	10
CGCAGAG	67590	0.0	40.436085	16
AGAGTAC	67850	0.0	39.90641	19
CAGTGGT	68715	0.0	39.62017	4
AGCAGTG	69095	0.0	39.5423	2
CAGAGTA	68885	0.0	39.437466	18
GCAGTGG	69340	0.0	39.344173	3
GCAGAGT	69700	0.0	39.166782	17
GTACATG	43190	0.0	38.78502	22
>>END_MODULE