FastQCFastQC Report
Thu 26 May 2016
SRR805439_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805439_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8048789
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT218220.2711215314502592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT124750.033.6913831
TCAACGC146850.029.11133612
CAACGCA149000.028.64597313
ATCAACG151900.028.20276611
ACGCAGA151750.028.20098915
AACGCAG152250.028.03448314
CGCAGAG155850.027.5457216
TATCAAC156550.027.37943310
GGTATCA131800.027.2287568
GTATCAA132650.026.9525079
AGTGGTA134050.026.8724375
TGGTATC134400.026.7857157
GTGGTAT136000.026.4871316
AGAGTAC160550.026.41700619
AGCAGTG166750.025.6101932
GCAGAGT171750.025.00873217
CAGAGTA173000.024.6329518
CAGTGGT147200.024.4412354
GCAGTGG147950.024.2108823
GAGTACT68300.023.8506620
Produced by FastQC (version 0.11.5)