##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805439_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8048789
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.16294339434168	37.0	35.0	37.0	33.0	37.0
2	35.43059744764088	37.0	35.0	37.0	33.0	37.0
3	37.2902837930029	39.0	37.0	39.0	34.0	39.0
4	37.42785889902195	39.0	37.0	39.0	34.0	39.0
5	37.3817215235733	39.0	37.0	39.0	34.0	39.0
6	37.35686213168217	39.0	37.0	39.0	34.0	39.0
7	37.37855371783258	39.0	37.0	39.0	34.0	39.0
8	38.37885003570102	40.0	38.0	40.0	35.0	40.0
9	31.902121300483838	40.0	33.0	40.0	3.0	40.0
10	34.24374747555191	39.0	33.0	40.0	17.0	40.0
11	36.63170148950358	39.0	34.0	40.0	32.0	40.0
12	37.48511434453059	39.0	37.0	40.0	33.0	40.0
13	37.86012330550596	39.0	38.0	40.0	34.0	40.0
14	37.88049320214507	40.0	38.0	40.0	34.0	40.0
15	37.801449137255304	40.0	38.0	40.0	34.0	40.0
16	37.89428484210482	40.0	38.0	40.0	34.0	40.0
17	37.83488957158648	40.0	38.0	40.0	34.0	40.0
18	37.732322216422865	40.0	37.0	40.0	33.0	40.0
19	37.50240775351423	39.0	37.0	40.0	33.0	40.0
20	37.32042932172778	39.0	37.0	40.0	32.0	40.0
21	37.29518142418692	39.0	37.0	40.0	32.0	40.0
22	37.09324682259654	39.0	37.0	40.0	32.0	40.0
23	37.01295263175616	39.0	36.0	40.0	32.0	40.0
24	36.777182008374176	38.0	36.0	40.0	31.0	40.0
25	36.64013505634202	38.0	36.0	40.0	31.0	40.0
26	36.43808403972324	38.0	36.0	40.0	31.0	40.0
27	36.280147734025576	38.0	36.0	40.0	30.0	40.0
28	36.19439309441458	38.0	35.0	40.0	30.0	40.0
29	36.05407148826985	38.0	35.0	40.0	30.0	40.0
30	35.76621563815377	38.0	35.0	39.0	30.0	40.0
31	35.511625910431995	38.0	34.0	39.0	30.0	40.0
32	35.298934535369234	38.0	34.0	39.0	29.0	40.0
33	35.1053396976862	37.0	34.0	39.0	29.0	40.0
34	34.79554452228776	37.0	34.0	38.0	27.0	40.0
35	34.54370502196045	37.0	33.0	38.0	27.0	40.0
36	34.30988810863349	37.0	33.0	38.0	26.0	40.0
37	34.191335740072205	36.0	33.0	38.0	26.0	40.0
38	34.08159749249235	36.0	33.0	38.0	26.0	39.0
39	33.92819578199901	36.0	33.0	38.0	26.0	39.0
40	33.729909679580366	36.0	32.0	38.0	26.0	39.0
41	33.45689668346381	36.0	32.0	38.0	25.0	39.0
42	33.008647884793604	35.0	31.0	38.0	24.0	39.0
43	32.86998665265048	35.0	31.0	38.0	24.0	39.0
44	32.576671720429	35.0	31.0	38.0	24.0	39.0
45	32.30820400435395	34.0	30.0	37.0	23.0	38.0
46	32.117581415042686	34.0	30.0	37.0	23.0	38.0
47	31.82142294946482	34.0	30.0	37.0	23.0	38.0
48	31.521175421544783	34.0	29.0	37.0	22.0	38.0
49	31.194115164405478	33.0	29.0	36.0	21.0	38.0
50	30.68334578034037	33.0	28.0	36.0	20.0	38.0
51	33.49134720763583	36.0	32.0	38.0	24.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	12.0
8	42.0
9	92.0
10	131.0
11	203.0
12	371.0
13	721.0
14	1164.0
15	1952.0
16	2978.0
17	4334.0
18	6765.0
19	10111.0
20	14799.0
21	21329.0
22	30040.0
23	40788.0
24	53419.0
25	67482.0
26	84634.0
27	103266.0
28	124712.0
29	150049.0
30	181046.0
31	220150.0
32	275753.0
33	362811.0
34	523650.0
35	877000.0
36	1555852.0
37	2106528.0
38	1209410.0
39	17194.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.573322769425316	29.30351385779898	23.794647865660288	32.32851550711542
2	23.30859959181437	25.498158791341158	30.531686195277324	20.661555421567147
3	17.11725826083899	25.683081020014313	31.89764323552276	25.302017483623935
4	17.75513061654368	27.816619369696483	31.415396775837955	23.012853237921878
5	23.116222825570407	28.26769343810603	29.046257766230422	19.56982597009314
6	22.564127348847137	26.683057538220968	29.600042938136404	21.152772174795487
7	23.017673838884335	27.52010271358834	29.03600777707056	20.42621567045676
8	21.743308216925552	25.900020487554094	29.47587519066533	22.88079610485503
9	19.555848712147878	35.71157102018701	26.6528666610592	18.079713606605914
10	21.85785961093029	26.54025344682287	29.074088039828105	22.527798902418734
11	21.7152915798886	27.819713002788372	28.121919955908897	22.343075461414134
12	21.839658115028236	26.74158808238109	28.76006564465785	22.658688157932826
13	22.145803548832006	27.055610477551344	27.11114678245386	23.687439191162795
14	22.52528920810323	28.042069434296263	27.015057793166154	22.417583564434352
15	22.288943094420787	27.82590026897214	27.107059211019198	22.778097425587873
16	22.716609914857997	26.798814082466322	26.726753552615186	23.7578224500605
17	24.059470312863215	26.406109043236192	26.828259505871006	22.706161138029586
18	22.589485200817165	26.61020185769561	26.74259444495315	24.057718496534076
19	23.175970944200426	27.51083423854197	26.650605451329383	22.662589365928216
20	23.962151324876327	26.571189777741722	26.800926201444714	22.66573269593724
21	22.90071214439837	27.584075070175153	26.84770342470153	22.66750936072495
22	23.822950259970785	26.456911716781246	26.902059427822	22.81807859542597
23	22.720660213604805	26.708489935566703	28.05076639479554	22.520083456032953
24	22.53112859586703	27.498335463881585	26.93058545825962	23.039950481991763
25	22.58854095939153	26.53775617673665	26.896456100414607	23.97724676345721
26	22.870496418778032	26.983226917738808	27.604488078889883	22.541788584593284
27	22.831459490365567	26.17111717054578	28.19084957998029	22.80657375910836
28	23.560699628229788	26.35878515389085	27.169130660525454	22.91138455735391
29	23.721456730944247	26.328196701391978	27.29825567548112	22.652090892182663
30	23.486663645922388	26.2408419452914	27.365110453261977	22.907383955524242
31	23.309730196679276	26.44869930122407	27.187170641446805	23.054399860649845
32	23.250665410659916	26.621073058319705	27.30815778622101	22.820103744799372
33	23.03701836388058	26.549161619219987	27.30931323954448	23.104506777354953
34	23.207789395398486	26.50985135776326	27.173864291882914	23.108494954955336
35	23.10510314036062	26.59003733356658	27.290937804432442	23.01392172164036
36	23.346369248839792	26.345329713575545	27.402544656096712	22.905756381487947
37	23.125988269788163	26.60820155678078	27.06157410760799	23.204236065823068
38	23.339598043879644	26.62487487248082	27.223983632817312	22.81154345082223
39	23.06468712249756	26.540762840223543	27.30241779229149	23.092132244987415
40	23.14443825027591	26.598908233275836	27.13813220846018	23.118521307988072
41	23.215877568662812	26.56721402437062	27.29153416743811	22.925374239528455
42	23.218996050213267	26.443071125358113	27.400295870596185	22.93763695383243
43	23.075409232370237	26.588086729568882	27.15167461838048	23.1848294196804
44	23.342318950092988	26.457445958640484	27.304939910836275	22.895295180430246
45	23.103748899368586	26.370389384042742	27.38879103427857	23.137070682310096
46	23.084280132079495	26.49890561176346	27.19870032622299	23.218113929934056
47	23.247584201797313	26.48117623657422	27.248248152610287	23.022991409018175
48	23.09361072827229	26.42628599159451	27.32027140977357	23.15983187035963
49	23.197986678492878	26.52241225357007	27.035880801447277	23.243720266489778
50	23.249137230457897	26.57716583202765	27.165316422135056	23.008380515379397
51	23.220139079307458	26.504036818458033	27.131075246226484	23.144748856008025
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4124.0
1	2831.5
2	1539.0
3	1296.0
4	1053.0
5	1093.0
6	1133.0
7	1353.5
8	1574.0
9	1927.0
10	2280.0
11	3214.0
12	4148.0
13	5512.0
14	6876.0
15	9592.0
16	12308.0
17	17226.0
18	22144.0
19	29670.0
20	37196.0
21	50565.0
22	63934.0
23	90690.5
24	117447.0
25	122017.5
26	145558.0
27	164528.0
28	183375.0
29	202222.0
30	221970.5
31	241719.0
32	262950.0
33	284181.0
34	308753.0
35	333325.0
36	364850.5
37	396376.0
38	419414.0
39	442452.0
40	465167.0
41	487882.0
42	504642.5
43	521403.0
44	532249.5
45	543096.0
46	548497.0
47	553898.0
48	555514.5
49	557131.0
50	551361.5
51	545592.0
52	530768.0
53	515944.0
54	489007.0
55	462070.0
56	426518.0
57	390966.0
58	354535.0
59	318104.0
60	280630.5
61	243157.0
62	207859.5
63	172562.0
64	144219.0
65	115876.0
66	92607.0
67	69338.0
68	55520.0
69	41702.0
70	31990.0
71	22278.0
72	16310.0
73	10342.0
74	7667.5
75	3802.5
76	2612.0
77	1968.5
78	1325.0
79	1006.0
80	687.0
81	544.0
82	401.0
83	281.0
84	161.0
85	111.5
86	62.0
87	48.5
88	35.0
89	26.0
90	17.0
91	11.0
92	5.0
93	3.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	8048789.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.01651057819721
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.0191079629266	49.79508452825472
2	13.157469964734112	16.582756904299586
3	3.576213545664401	6.760814961907586
4	1.4333813247395377	3.6130675765215767
5	0.7237532589685778	2.2804202449899047
6	0.45335427679843754	1.7141282747723814
7	0.2986341991037848	1.3173219616784577
8	0.2050534054444152	1.0337400074626684
9	0.17237545928896417	0.9776249958334154
>10	0.8507768881296082	9.74349693064534
>50	0.0884772244081877	3.858933501870891
>100	0.021208331369257927	1.827810182033283
>500	6.472091823179122E-5	0.03227426329548033
>1k	1.0786458847292712E-4	0.1658664927438914
>5k	0.0	0.0
>10k+	2.1572917370340553E-5	0.29665917369084704
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21822	0.2711215314502592	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.212114642339363E-5	0.0	0.0	0.0	1.2424229284678727E-5
2	8.696960499275109E-5	0.0	0.0	0.0	1.2424229284678727E-5
3	9.939383427742982E-5	0.0	0.0	0.0	1.2424229284678727E-5
4	9.939383427742982E-5	0.0	0.0	0.0	1.2424229284678727E-5
5	2.1121189783953833E-4	0.0	0.0	1.2424229284678727E-5	2.4848458569357454E-5
6	2.2363612712421706E-4	1.2424229284678727E-5	0.0	1.2424229284678727E-5	2.4848458569357454E-5
7	2.2363612712421706E-4	1.2424229284678727E-5	0.0	1.2424229284678727E-5	2.4848458569357454E-5
8	2.2363612712421706E-4	2.4848458569357454E-5	0.0	1.2424229284678727E-5	2.4848458569357454E-5
9	2.2363612712421706E-4	2.4848458569357454E-5	0.0	1.2424229284678727E-5	2.4848458569357454E-5
10	2.484845856935745E-4	2.4848458569357454E-5	0.0	1.2424229284678727E-5	3.727268785403618E-5
11	2.6090881497825325E-4	3.727268785403618E-5	0.0	1.2424229284678727E-5	3.727268785403618E-5
12	2.73333044262932E-4	3.727268785403618E-5	0.0	2.4848458569357454E-5	3.727268785403618E-5
13	3.4787841997100435E-4	3.727268785403618E-5	0.0	2.4848458569357454E-5	3.727268785403618E-5
14	3.727268785403618E-4	4.969691713871491E-5	0.0	2.4848458569357454E-5	3.727268785403618E-5
15	3.9757533710971926E-4	7.454537570807236E-5	0.0	2.4848458569357454E-5	3.727268785403618E-5
16	4.0999956639439794E-4	7.454537570807236E-5	0.0	2.4848458569357454E-5	3.727268785403618E-5
17	4.2242379567907667E-4	7.454537570807236E-5	0.0	2.4848458569357454E-5	3.727268785403618E-5
18	4.472722542484341E-4	7.454537570807236E-5	0.0	2.4848458569357454E-5	3.727268785403618E-5
19	4.721207128177916E-4	8.696960499275109E-5	0.0	2.4848458569357454E-5	3.727268785403618E-5
20	4.845449421024703E-4	8.696960499275109E-5	0.0	2.4848458569357454E-5	4.969691713871491E-5
21	4.96969171387149E-4	8.696960499275109E-5	0.0	2.4848458569357454E-5	6.212114642339363E-5
22	4.96969171387149E-4	8.696960499275109E-5	0.0	2.4848458569357454E-5	9.939383427742982E-5
23	4.96969171387149E-4	8.696960499275109E-5	0.0	2.4848458569357454E-5	1.1181806356210853E-4
24	5.342418592411852E-4	8.696960499275109E-5	0.0	2.4848458569357454E-5	1.36666522131466E-4
25	5.839387763799001E-4	8.696960499275109E-5	0.0	2.4848458569357454E-5	1.36666522131466E-4
26	6.087872349492576E-4	8.696960499275109E-5	0.0	3.727268785403618E-5	1.36666522131466E-4
27	6.460599228032938E-4	9.939383427742982E-5	0.0	4.969691713871491E-5	1.36666522131466E-4
28	6.460599228032938E-4	9.939383427742982E-5	0.0	4.969691713871491E-5	1.4909075141614472E-4
29	6.584841520879725E-4	9.939383427742982E-5	0.0	4.969691713871491E-5	1.6151498070082345E-4
30	6.584841520879725E-4	9.939383427742982E-5	0.0	6.212114642339363E-5	1.863634392701809E-4
31	6.8333261065733E-4	9.939383427742982E-5	0.0	6.212114642339363E-5	2.1121189783953833E-4
32	7.081810692266874E-4	9.939383427742982E-5	0.0	6.212114642339363E-5	2.1121189783953833E-4
33	7.578779863654023E-4	9.939383427742982E-5	0.0	8.696960499275109E-5	2.484845856935745E-4
34	7.827264449347598E-4	1.1181806356210853E-4	0.0	9.939383427742982E-5	2.484845856935745E-4
35	8.075749035041173E-4	1.2424229284678726E-4	0.0	1.1181806356210853E-4	2.73333044262932E-4
36	8.324233620734746E-4	1.36666522131466E-4	0.0	1.1181806356210853E-4	2.9818150283228943E-4
37	8.945445084968682E-4	1.36666522131466E-4	0.0	1.2424229284678726E-4	3.1060573211696816E-4
38	9.442414256355832E-4	1.36666522131466E-4	0.0	1.2424229284678726E-4	3.1060573211696816E-4
39	0.0010063625720589768	1.36666522131466E-4	0.0	1.2424229284678726E-4	3.1060573211696816E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	12475	0.0	33.691383	1
TCAACGC	14685	0.0	29.111336	12
CAACGCA	14900	0.0	28.645973	13
ATCAACG	15190	0.0	28.202766	11
ACGCAGA	15175	0.0	28.200989	15
AACGCAG	15225	0.0	28.034483	14
CGCAGAG	15585	0.0	27.54572	16
TATCAAC	15655	0.0	27.379433	10
GGTATCA	13180	0.0	27.228756	8
GTATCAA	13265	0.0	26.952507	9
AGTGGTA	13405	0.0	26.872437	5
TGGTATC	13440	0.0	26.785715	7
GTGGTAT	13600	0.0	26.487131	6
AGAGTAC	16055	0.0	26.417006	19
AGCAGTG	16675	0.0	25.610193	2
GCAGAGT	17175	0.0	25.008732	17
CAGAGTA	17300	0.0	24.63295	18
CAGTGGT	14720	0.0	24.441235	4
GCAGTGG	14795	0.0	24.210882	3
GAGTACT	6830	0.0	23.85066	20
>>END_MODULE