FastQCFastQC Report
Thu 26 May 2016
SRR805438_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805438_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20509353
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4486582.187577540841976No Hit
AAGCAGTGATATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT730430.3561448281669344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1742800.042.730381
TCAACGC1782200.041.81601712
CAACGCA1782650.041.69943613
ATCAACG1789950.041.62239511
AACGCAG1791500.041.483414
ACGCAGA1791300.041.471715
TATCAAC1801850.041.3612410
CGCAGAG1804250.041.2064616
GTATCAA1537500.041.193669
GGTATCA1540800.041.1915938
GTGGTAT1540800.041.1594666
TGGTATC1542450.041.147537
AGTGGTA1540950.041.1408585
AGCAGTG1813600.041.1056752
AGAGTAC1795300.040.89929619
CAGAGTA1822600.040.4706218
GCAGAGT1836300.040.35370317
GCAGTGG1570850.040.3233953
GAGTACT763400.040.19288620
CAGTGGT1581500.040.1229864
Produced by FastQC (version 0.11.5)