##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805438_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20509353
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.227965943147986	37.0	36.0	37.0	35.0	37.0
2	36.21567038219099	37.0	37.0	37.0	35.0	37.0
3	37.96341639836225	39.0	38.0	39.0	35.0	39.0
4	37.91489360975941	39.0	38.0	39.0	35.0	39.0
5	37.76588164434051	39.0	38.0	39.0	35.0	39.0
6	37.743257478673264	39.0	38.0	39.0	35.0	39.0
7	37.70283977266372	39.0	37.0	39.0	35.0	39.0
8	38.72956455525438	40.0	39.0	40.0	36.0	40.0
9	33.0278990760947	40.0	36.0	40.0	3.0	40.0
10	34.92518881507379	40.0	35.0	40.0	17.0	40.0
11	37.16500471760372	40.0	36.0	40.0	32.0	40.0
12	37.91643895348625	40.0	37.0	40.0	34.0	40.0
13	38.28408955660376	40.0	38.0	40.0	35.0	40.0
14	38.34639859190097	40.0	38.0	40.0	35.0	40.0
15	38.24971455706087	40.0	38.0	40.0	35.0	40.0
16	38.30120643006145	40.0	38.0	40.0	35.0	40.0
17	38.16748870625027	40.0	38.0	40.0	34.0	40.0
18	38.13796739468086	40.0	38.0	40.0	34.0	40.0
19	37.95001114857207	40.0	38.0	40.0	34.0	40.0
20	37.808744868743545	40.0	38.0	40.0	34.0	40.0
21	37.65862492102993	39.0	37.0	40.0	34.0	40.0
22	37.59915439555797	39.0	37.0	40.0	34.0	40.0
23	37.30908683467489	39.0	37.0	40.0	33.0	40.0
24	37.223993609159685	39.0	37.0	40.0	33.0	40.0
25	37.084888538414646	38.0	36.0	40.0	33.0	40.0
26	36.91763465185859	38.0	36.0	40.0	32.0	40.0
27	36.83357680761553	38.0	36.0	40.0	32.0	40.0
28	36.746148891191254	38.0	36.0	40.0	32.0	40.0
29	36.55554902195111	38.0	36.0	40.0	31.0	40.0
30	36.343899927023536	38.0	35.0	40.0	31.0	40.0
31	36.08517138497738	38.0	35.0	39.0	30.0	40.0
32	35.8592860535386	38.0	35.0	39.0	30.0	40.0
33	35.62270204233161	38.0	34.0	39.0	30.0	40.0
34	35.36143992450664	37.0	34.0	39.0	30.0	40.0
35	35.14522681432223	37.0	34.0	38.0	29.0	40.0
36	34.92967013635194	37.0	34.0	38.0	29.0	40.0
37	34.75826404665228	37.0	33.0	38.0	29.0	40.0
38	34.52484181241602	36.0	33.0	38.0	28.0	40.0
39	34.38860104460633	36.0	33.0	38.0	28.0	39.0
40	34.19440574258974	36.0	33.0	38.0	27.0	39.0
41	33.92557868597805	36.0	33.0	38.0	27.0	39.0
42	33.57060824883164	35.0	32.0	38.0	26.0	39.0
43	33.412289700216284	35.0	32.0	38.0	26.0	39.0
44	33.07198730257361	35.0	31.0	38.0	25.0	39.0
45	32.800694639172676	35.0	31.0	37.0	24.0	39.0
46	32.602926284412774	34.0	31.0	37.0	24.0	38.0
47	32.35405173434774	34.0	31.0	37.0	24.0	38.0
48	32.019932808216815	34.0	30.0	37.0	23.0	38.0
49	31.62604480989722	34.0	29.0	36.0	23.0	38.0
50	31.16296559915859	33.0	29.0	36.0	22.0	38.0
51	33.813336578681934	36.0	32.0	38.0	26.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	25.0
8	127.0
9	259.0
10	316.0
11	352.0
12	366.0
13	370.0
14	433.0
15	662.0
16	1206.0
17	2487.0
18	5010.0
19	9940.0
20	17539.0
21	29357.0
22	45133.0
23	65626.0
24	90588.0
25	119922.0
26	154860.0
27	194842.0
28	241833.0
29	295636.0
30	364608.0
31	454017.0
32	584537.0
33	797182.0
34	1193962.0
35	2083062.0
36	3966347.0
37	6057097.0
38	3675557.0
39	56094.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.750662929249888	36.423396681504286	25.870840489214846	23.955099900030977
2	24.968881270901136	37.50427914522706	19.61336859334373	17.913470990528076
3	30.8307092866362	25.82678254160431	21.911900390031807	21.430607781727684
4	20.839609128576605	24.559521697247106	23.334114927955067	31.266754246221222
5	21.294513776226875	32.98467777116129	24.786574203486573	20.934234249125264
6	29.76653139667546	24.606134576746523	23.689825807766827	21.937508218811193
7	22.258244811525746	24.35291352194289	32.33035678892455	21.058484877606816
8	28.922701754657986	25.24022088848927	24.753091918599285	21.083985438253464
9	25.62415303885988	34.29825894556498	22.423744912869754	17.653843102705384
10	20.969374314245798	25.257237515001087	32.623223170423756	21.150165000329363
11	20.86953206178664	33.23053633139963	24.822138465313852	21.077793141499882
12	20.822031782280018	25.08574014987211	32.720661641544716	21.37156642630316
13	20.84554788247099	25.31711263636644	24.651933193601963	29.185406287560607
14	21.27142674856686	33.36067695553341	24.37382593200283	20.994070363896903
15	21.161257500419442	32.964599127042185	24.47552587348806	21.398617499050314
16	21.392288679218694	24.88486594384523	24.488573579088527	29.23427179784755
17	29.55015694546776	24.72423679089243	24.47815881856439	21.247447445075423
18	21.334363887539503	24.911468440764562	24.41455856749845	29.339609104197486
19	21.696496227842974	32.761282133083384	24.43272101270089	21.109500626372757
20	29.544008531132114	24.974673750069055	24.443106518279734	21.0382112005191
21	21.522565826430508	32.810552336780205	24.459586804127852	21.207295032661442
22	29.310144498463703	24.682411970772556	24.77339972645651	21.234043804307234
23	21.36908463177751	24.91788990125627	32.58495770198114	21.12806776498508
24	21.32236935996957	32.56387951389788	24.57842526773029	21.535325858402263
25	21.19727521389875	24.828101598329308	24.73509525142017	29.239527936351774
26	21.673267801280712	29.19092084474825	28.09997467984485	21.03583667412619
27	21.623836695384785	24.60882603171343	32.52999253560071	21.237344737301076
28	26.108668566970394	24.658964132120598	27.956250009446908	21.276117291462096
29	26.184180456594607	24.760576308769956	27.857860752603948	21.19738248203149
30	26.09146178331418	24.6347751681879	27.894907264992707	21.378855783505212
31	24.050841584324967	25.467687839787047	28.411886030729494	22.069584545158495
32	23.18414920256139	26.237751137249433	28.609283774090777	21.968815886098405
33	22.98072981629406	25.795523632559252	28.850315268355857	22.373431282790833
34	22.644458847629174	25.685885849251317	29.088231110947284	22.581424192172225
35	22.57522214377021	25.881942740953356	29.127120684889473	22.415714430386956
36	22.84354850199321	25.727774055085987	29.123844131016714	22.304833311904083
37	22.847356520705457	25.75584905091838	29.037156852290757	22.359637576085408
38	23.041097395905176	25.811321303017216	28.997794323399674	22.14978697767794
39	22.904920501392706	25.75608309048072	28.986233744184908	22.352762663941668
40	22.645492522362844	25.940408749120465	28.890769981871202	22.523328746645493
41	22.8554016306609	26.11051650434804	28.825692356067982	22.208389508923077
42	22.858044327385656	26.012639209047695	28.898015456655312	22.231301006911337
43	22.68126156880717	25.911860798339177	29.13881778718227	22.268059845671388
44	23.02556301995485	25.739788085952785	28.93207308880002	22.302575805292346
45	22.823859923811344	25.587130905592193	29.02448458515488	22.56452458544158
46	22.679028441316508	25.70347294719634	28.89730846214408	22.720190149343082
47	22.770937727777177	25.80004352160695	28.831523841829625	22.59749490878625
48	22.807199232467255	25.690517882255964	28.864347890447835	22.637934994828946
49	22.61016717592213	26.069725358961836	28.76582211052684	22.554285354589197
50	22.958091364461865	25.97128734387672	28.656618275574075	22.414003016087346
51	22.852373743823122	25.837328949382265	28.70994028919391	22.6003570176007
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11858.0
1	7342.5
2	2827.0
3	2191.0
4	1555.0
5	1558.5
6	1562.0
7	1895.0
8	2228.0
9	2874.0
10	3520.0
11	5313.5
12	7107.0
13	10131.0
14	13155.0
15	18543.5
16	23932.0
17	33586.0
18	43240.0
19	58999.0
20	74758.0
21	143290.5
22	211823.0
23	448515.5
24	685208.0
25	509692.5
26	353011.0
27	371845.0
28	410021.0
29	448197.0
30	491410.0
31	534623.0
32	584802.0
33	634981.0
34	696927.5
35	758874.0
36	833646.0
37	908418.0
38	968919.0
39	1029420.0
40	1089812.0
41	1150204.0
42	1204878.5
43	1259553.0
44	1308719.5
45	1357886.0
46	1391032.5
47	1424179.0
48	1427737.5
49	1431296.0
50	1421219.5
51	1411143.0
52	1378556.0
53	1345969.0
54	1289128.5
55	1232288.0
56	1133718.5
57	1035149.0
58	931519.0
59	827889.0
60	730138.0
61	632387.0
62	548401.5
63	464416.0
64	393204.0
65	321992.0
66	263522.5
67	205053.0
68	167775.5
69	130498.0
70	103057.0
71	75616.0
72	59364.5
73	43113.0
74	34119.0
75	20024.5
76	14924.0
77	11552.5
78	8181.0
79	6404.0
80	4627.0
81	3663.5
82	2700.0
83	1914.0
84	1128.0
85	782.0
86	436.0
87	298.0
88	160.0
89	123.0
90	86.0
91	57.0
92	28.0
93	19.5
94	11.0
95	8.5
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.0509353E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.18662626577124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.43854779982685	35.35073073133573
2	16.85190520390949	16.91480536669622
3	5.825514485369199	8.770887548491821
4	2.4210990456173906	4.8602717181926165
5	1.1870480001357189	2.978696717117125
6	0.6641768824298804	1.99996781837241
7	0.41625006145587057	1.4623130387173062
8	0.30224098742460276	1.2134764382460965
9	0.23478485068879879	1.0604753598945282
>10	1.500202849984766	13.738744490070815
>50	0.1026911803433291	3.613421695788699
>100	0.05439431181381521	4.364595237405076
>500	6.330235002557577E-4	0.21007030560026146
>1k	4.111339036175289E-4	0.38329137802784197
>5k	5.009179823977882E-5	0.20874235755508255
>10k+	5.009179823977882E-5	2.8695097984882514
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	448658	2.187577540841976	No Hit
AAGCAGTGATATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	73043	0.3561448281669344	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.264066009298294E-5	0.0	0.0	0.0	0.0
2	1.3164725381634417E-4	0.0	0.0	0.0	0.0
3	1.3164725381634417E-4	4.875824215420155E-6	0.0	0.0	0.0
4	1.3164725381634417E-4	4.875824215420155E-6	0.0	4.875824215420155E-6	0.0
5	1.3164725381634417E-4	9.75164843084031E-6	0.0	4.875824215420155E-6	4.875824215420155E-6
6	1.3164725381634417E-4	9.75164843084031E-6	0.0	4.875824215420155E-6	9.75164843084031E-6
7	1.3164725381634417E-4	9.75164843084031E-6	0.0	4.875824215420155E-6	9.75164843084031E-6
8	1.3164725381634417E-4	9.75164843084031E-6	0.0	9.75164843084031E-6	9.75164843084031E-6
9	1.3164725381634417E-4	9.75164843084031E-6	0.0	9.75164843084031E-6	9.75164843084031E-6
10	1.3164725381634417E-4	9.75164843084031E-6	4.875824215420155E-6	1.4627472646260465E-5	1.950329686168062E-5
11	1.3164725381634417E-4	2.4379121077100773E-5	4.875824215420155E-6	1.4627472646260465E-5	3.900659372336124E-5
12	1.3164725381634417E-4	3.4130769507941085E-5	4.875824215420155E-6	1.4627472646260465E-5	3.900659372336124E-5
13	1.4139890224718448E-4	3.4130769507941085E-5	4.875824215420155E-6	1.4627472646260465E-5	3.900659372336124E-5
14	1.4139890224718448E-4	3.4130769507941085E-5	4.875824215420155E-6	1.950329686168062E-5	5.850989058504186E-5
15	1.4627472646260465E-4	3.4130769507941085E-5	4.875824215420155E-6	2.4379121077100773E-5	5.850989058504186E-5
16	1.4627472646260465E-4	3.4130769507941085E-5	4.875824215420155E-6	2.4379121077100773E-5	6.338571480046202E-5
17	1.7552967175512557E-4	3.900659372336124E-5	4.875824215420155E-6	2.925494529252093E-5	6.826153901588217E-5
18	1.8040549597054574E-4	3.900659372336124E-5	4.875824215420155E-6	2.925494529252093E-5	6.826153901588217E-5
19	2.047846170476465E-4	3.900659372336124E-5	4.875824215420155E-6	2.925494529252093E-5	6.826153901588217E-5
20	2.047846170476465E-4	3.900659372336124E-5	4.875824215420155E-6	3.4130769507941085E-5	6.826153901588217E-5
21	2.1941208969390697E-4	3.900659372336124E-5	4.875824215420155E-6	3.4130769507941085E-5	6.826153901588217E-5
22	2.2428791390932714E-4	3.900659372336124E-5	4.875824215420155E-6	3.4130769507941085E-5	6.826153901588217E-5
23	2.3403956234016745E-4	3.900659372336124E-5	9.75164843084031E-6	3.4130769507941085E-5	6.826153901588217E-5
24	2.486670349864279E-4	4.388241793878139E-5	9.75164843084031E-6	3.4130769507941085E-5	7.801318744672248E-5
25	2.6817033184810854E-4	4.388241793878139E-5	9.75164843084031E-6	3.4130769507941085E-5	7.801318744672248E-5
26	3.2180439821773025E-4	4.388241793878139E-5	9.75164843084031E-6	3.4130769507941085E-5	7.801318744672248E-5
27	3.5105934351025114E-4	5.36340663696217E-5	9.75164843084031E-6	3.4130769507941085E-5	9.264066009298294E-5
28	3.803142888027721E-4	5.36340663696217E-5	9.75164843084031E-6	3.900659372336124E-5	9.75164843084031E-5
29	4.144450583107132E-4	6.338571480046202E-5	9.75164843084031E-6	4.388241793878139E-5	9.75164843084031E-5
30	4.485758278186543E-4	6.338571480046202E-5	9.75164843084031E-6	4.388241793878139E-5	9.75164843084031E-5
31	5.509681363424776E-4	6.338571480046202E-5	9.75164843084031E-6	4.388241793878139E-5	9.75164843084031E-5
32	7.021186870205023E-4	6.826153901588217E-5	9.75164843084031E-6	4.388241793878139E-5	1.0239230852382325E-4
33	8.435175892676868E-4	6.826153901588217E-5	9.75164843084031E-6	4.388241793878139E-5	1.072681327392434E-4
34	9.264066009298294E-4	6.826153901588217E-5	9.75164843084031E-6	4.388241793878139E-5	1.2189560538550388E-4
35	9.654131946531906E-4	6.826153901588217E-5	9.75164843084031E-6	4.875824215420155E-5	1.3652307803176434E-4
36	0.0010531780305307534	6.826153901588217E-5	1.4627472646260465E-5	4.875824215420155E-5	1.5115055067802481E-4
37	0.0011506945148391566	6.826153901588217E-5	1.4627472646260465E-5	4.875824215420155E-5	1.6090219910886512E-4
38	0.0012482109991475596	7.313736323130232E-5	1.4627472646260465E-5	5.36340663696217E-5	1.6577802332428526E-4
39	0.0013603549561022233	7.801318744672248E-5	1.4627472646260465E-5	5.36340663696217E-5	1.6577802332428526E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	174280	0.0	42.73038	1
TCAACGC	178220	0.0	41.816017	12
CAACGCA	178265	0.0	41.699436	13
ATCAACG	178995	0.0	41.622395	11
AACGCAG	179150	0.0	41.4834	14
ACGCAGA	179130	0.0	41.4717	15
TATCAAC	180185	0.0	41.36124	10
CGCAGAG	180425	0.0	41.20646	16
GTATCAA	153750	0.0	41.19366	9
GGTATCA	154080	0.0	41.191593	8
GTGGTAT	154080	0.0	41.159466	6
TGGTATC	154245	0.0	41.14753	7
AGTGGTA	154095	0.0	41.140858	5
AGCAGTG	181360	0.0	41.105675	2
AGAGTAC	179530	0.0	40.899296	19
CAGAGTA	182260	0.0	40.47062	18
GCAGAGT	183630	0.0	40.353703	17
GCAGTGG	157085	0.0	40.323395	3
GAGTACT	76340	0.0	40.192886	20
CAGTGGT	158150	0.0	40.122986	4
>>END_MODULE