FastQCFastQC Report
Thu 26 May 2016
SRR805437_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805437_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22034430
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3588251.628474165204183No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA292360.1326832597893388No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT253830.11519698943880101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT988050.041.021711
TCAACGC1023400.039.21560712
CAACGCA1024100.039.21296713
AACGCAG1032650.038.9035514
ACGCAGA1023550.038.86253715
ATCAACG1054600.038.8618911
GGTATCA1050350.038.8177768
GTATCAA1055950.038.6204389
AGTGGTA1047250.038.6125565
TGGTATC1049150.038.6112567
GTGGTAT1055600.038.415836
CGCAGAG998850.038.28502716
TATCAAC1078250.037.98863610
AGAGTAC1009650.037.875519
CAGTGGT1076850.037.5992474
GCAGTGG1079800.037.5652923
AGCAGTG1085500.037.3410872
GAGTACT560200.036.77436420
GCAGAGT1034350.036.76656717
CAGAGTA1029800.036.75203718
Produced by FastQC (version 0.11.5)