FastQCFastQC Report
Thu 26 May 2016
SRR805436_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805436_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20975005
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4997942.382807536875438No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA273650.1304648079940863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC1345150.042.0159412
CAACGCA1346400.041.99030713
AAGCAGT1371800.041.9804271
ATCAACG1388050.041.92014311
ACGCAGA1346450.041.80827315
AACGCAG1355050.041.7621114
CGCAGAG1311000.041.52974716
GTGGTAT1387850.041.5030446
GTATCAA1394250.041.465849
GGTATCA1395400.041.4219938
TGGTATC1390700.041.3629847
AGTGGTA1388750.041.3449135
TATCAAC1409350.041.2291110
AGAGTAC1303450.041.16613819
GAGTACT692500.040.5812320
AGCAGTG1421850.040.448892
CAGAGTA1334850.040.35790618
GCAGAGT1345300.040.24009317
CAGTGGT1431400.040.16334
GCAGTGG1453650.039.6011773
Produced by FastQC (version 0.11.5)