##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805436_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20975005
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.65496928367836	35.0	35.0	35.0	33.0	37.0
2	35.27067330854033	36.0	35.0	37.0	35.0	37.0
3	37.83231293627821	39.0	38.0	39.0	35.0	39.0
4	37.98181235236893	39.0	38.0	39.0	35.0	39.0
5	37.96889740908286	39.0	39.0	39.0	35.0	39.0
6	38.03961376886442	39.0	39.0	39.0	35.0	39.0
7	37.89360035909407	39.0	38.0	39.0	35.0	39.0
8	38.919650984588564	40.0	39.0	40.0	37.0	40.0
9	38.78645473505251	40.0	39.0	40.0	36.0	40.0
10	38.74994332540088	40.0	39.0	40.0	36.0	40.0
11	38.88423454487854	40.0	39.0	40.0	36.0	40.0
12	38.88580808443192	40.0	39.0	40.0	37.0	40.0
13	38.94932778323533	40.0	40.0	40.0	37.0	40.0
14	38.95294127462663	40.0	40.0	40.0	37.0	40.0
15	38.91754052025256	40.0	40.0	40.0	37.0	40.0
16	38.863261105301284	40.0	39.0	40.0	37.0	40.0
17	38.84002201668128	40.0	39.0	40.0	36.0	40.0
18	38.06867173571592	40.0	39.0	40.0	34.0	40.0
19	38.48112131558491	40.0	39.0	40.0	35.0	40.0
20	38.546377176072184	40.0	39.0	40.0	36.0	40.0
21	38.40641592218929	40.0	39.0	40.0	35.0	40.0
22	36.872877598837285	40.0	38.0	40.0	31.0	40.0
23	36.95143090549919	40.0	38.0	40.0	30.0	40.0
24	37.84390201575637	40.0	38.0	40.0	33.0	40.0
25	36.25964241724853	40.0	38.0	40.0	30.0	40.0
26	36.826771102080784	40.0	38.0	40.0	30.0	40.0
27	37.6957015743262	40.0	38.0	40.0	31.0	40.0
28	38.205848866305395	40.0	38.0	40.0	35.0	40.0
29	38.36957902989773	40.0	39.0	40.0	35.0	40.0
30	38.34622056109164	40.0	39.0	40.0	35.0	40.0
31	38.42281472638505	40.0	39.0	40.0	35.0	40.0
32	38.4181455975815	40.0	39.0	40.0	35.0	40.0
33	38.26001772109232	40.0	39.0	40.0	35.0	40.0
34	38.31987849347354	40.0	39.0	40.0	35.0	40.0
35	38.30252607806291	40.0	39.0	40.0	35.0	40.0
36	38.22872819339018	40.0	39.0	40.0	35.0	40.0
37	38.067453476173185	40.0	38.0	40.0	35.0	40.0
38	38.025071603081855	40.0	38.0	40.0	34.0	40.0
39	37.46998868415049	40.0	38.0	40.0	33.0	40.0
40	37.67521981520386	40.0	38.0	40.0	33.0	40.0
41	37.732517203214016	40.0	38.0	40.0	33.0	40.0
42	37.00784838907071	40.0	37.0	40.0	31.0	40.0
43	37.358330736989096	40.0	37.0	40.0	33.0	40.0
44	37.506409462119315	40.0	37.0	40.0	33.0	40.0
45	37.51101322741044	40.0	37.0	40.0	33.0	40.0
46	37.5062449806329	40.0	37.0	40.0	33.0	40.0
47	37.427452293813516	40.0	37.0	40.0	33.0	40.0
48	37.22383274759648	40.0	37.0	40.0	33.0	40.0
49	37.13264416385121	40.0	36.0	40.0	32.0	40.0
50	36.380375737693505	40.0	35.0	40.0	30.0	40.0
51	36.09717890412899	39.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	14.0
8	87.0
9	217.0
10	286.0
11	329.0
12	406.0
13	499.0
14	673.0
15	961.0
16	1581.0
17	2704.0
18	4528.0
19	7665.0
20	12434.0
21	19382.0
22	27401.0
23	37746.0
24	50170.0
25	63880.0
26	80865.0
27	100930.0
28	124453.0
29	154481.0
30	190849.0
31	239853.0
32	307255.0
33	410577.0
34	582639.0
35	884829.0
36	1415795.0
37	2003013.0
38	3438435.0
39	1.0810067E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.828688002696543	39.94622170531068	23.113458137435487	23.11163215455729
2	25.838906832203378	39.382326726501375	20.13126099373993	14.647505447555318
3	26.621976967347567	26.887826725190294	25.160127494606083	21.330068812856066
4	18.25009338495986	27.990324674535238	25.33708096851467	28.42250097199023
5	18.824729719969078	34.00222312223525	27.708355731023666	19.464691426772006
6	26.213505074253856	26.096942527546478	26.161486016332297	21.52806638186737
7	21.732643210335347	26.312713632249434	31.6856610999616	20.26898205745362
8	25.47788665604609	26.54388878572377	27.38166212594467	20.596562432285477
9	26.237376343891217	25.535111910581186	27.683292566557192	20.544219178970398
10	20.532767453452337	26.628003187603532	32.02701977901793	20.812209579926204
11	19.946264613524527	32.67623535727405	26.638510932416942	20.738989096784483
12	20.075041698440597	26.402549129308913	32.39539156248115	21.127017609769343
13	20.099880786679194	26.92192922004071	26.269986586415595	26.708203406864506
14	20.35392601813444	33.17572987467703	26.06469462105015	20.405649486138383
15	20.399661406516948	32.95106246696962	25.945710144050025	20.703565982463413
16	20.95133707953824	26.79001506793443	25.852327567979128	26.4063202845482
17	26.82185773018886	26.627903068437885	25.906291798261787	20.643947403111465
18	20.991832898251992	26.495087843840796	25.57740033911792	26.93567891878929
19	21.094941336128407	32.548745518773416	25.71649923325406	20.639813911844122
20	27.11969317766551	26.579917382618024	25.74058027638134	20.55980916333512
21	21.020347790143553	32.39385163436195	25.860470593451584	20.72532998204291
22	27.034839800991705	26.796298737473485	26.488575330494555	19.680286131040255
23	20.57918937325641	26.3099770417218	32.36408763668948	20.74674594833231
24	20.686483745772648	32.413918375704796	25.984322768933787	20.91527510958877
25	19.36767595526199	30.632531434438278	24.872775954046254	25.12701665625348
26	20.88885318501712	29.132641446331004	29.489580574593425	20.488924794058452
27	20.89942767594096	26.47022491770562	32.04830702066579	20.58204038568763
28	23.635908549247066	26.580894736377893	29.13444835889193	20.64874835548311
29	23.846859631261115	26.33455868067731	29.16527552675196	20.653306161309615
30	23.877405511941475	26.037686284222577	29.153499605840377	20.931408597995567
31	22.532604878997645	26.727001972109182	29.424627073986393	21.315766074906776
32	21.773496597497832	27.266677647991028	29.801118045025493	21.158707709485647
33	21.914645550740037	26.85691850848188	29.88058405707174	21.347851883706344
34	21.51723920923976	27.0341723398874	30.02610011296779	21.422488337905044
35	21.408857828639373	27.283049515363643	30.049151358962728	21.258941297034255
36	21.644752885636976	27.00151918914918	30.019087003793327	21.33464092142052
37	21.71193284578478	27.233261684562173	29.87697976710852	21.177825702544528
38	21.71667181962531	27.183135355629233	30.00220023785453	21.09799258689092
39	21.985210492202505	27.13477303104338	29.635506642310695	21.244509834443427
40	21.695904244122946	27.203693157641677	29.627039421444717	21.47336317679066
41	21.672686132851933	27.29176465035408	29.68501795351181	21.35053126328218
42	21.70998767342368	27.20926169028327	29.737642494006554	21.3431081422865
43	21.351217794703743	27.11011987839812	30.265623297825194	21.273039029072937
44	21.532996058880556	26.987826701352397	30.09734681827251	21.381830421494534
45	21.655665874692282	26.951602633706166	30.00835995033136	21.38437154127019
46	21.590226080994977	27.074768277766797	29.843072743010072	21.491932898228153
47	21.572776740696845	27.170253356316245	29.867106110344192	21.389863792642718
48	21.787231993508463	27.061829067502007	29.840598369344846	21.31034056964468
49	21.612080664581487	27.35106856947114	29.515296897426246	21.521553868521128
50	21.80908180951566	27.11965026945166	29.699954779510186	21.371313141522492
51	21.863265348446877	27.133047167330833	29.49641251575387	21.507274968468423
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	29523.0
1	18025.5
2	6528.0
3	5415.0
4	4302.0
5	4495.5
6	4689.0
7	5544.0
8	6399.0
9	8134.5
10	9870.0
11	14289.5
12	18709.0
13	25794.5
14	32880.0
15	43755.5
16	54631.0
17	74119.0
18	93607.0
19	128513.0
20	163419.0
21	211230.5
22	259042.0
23	566104.5
24	873167.0
25	679888.5
26	519849.0
27	553088.0
28	603650.0
29	654212.0
30	712448.0
31	770684.0
32	829053.0
33	887422.0
34	943655.5
35	999889.0
36	1051251.0
37	1102613.0
38	1132210.0
39	1161807.0
40	1179983.5
41	1198160.0
42	1209658.0
43	1221156.0
44	1232011.5
45	1242867.0
46	1251580.0
47	1260293.0
48	1242542.0
49	1224791.0
50	1211360.5
51	1197930.0
52	1170531.0
53	1143132.0
54	1099526.5
55	1055921.0
56	983341.0
57	910761.0
58	819836.0
59	728911.0
60	639981.5
61	551052.0
62	473613.5
63	396175.0
64	334298.5
65	272422.0
66	220872.5
67	169323.0
68	137306.0
69	105289.0
70	81752.0
71	58215.0
72	44182.5
73	30150.0
74	23308.0
75	12624.5
76	8783.0
77	6680.0
78	4577.0
79	3582.0
80	2587.0
81	2060.5
82	1534.0
83	1106.5
84	679.0
85	514.5
86	350.0
87	269.5
88	189.0
89	140.0
90	91.0
91	66.5
92	42.0
93	36.5
94	31.0
95	24.0
96	17.0
97	14.0
98	11.0
99	10.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.0975005E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.13962707907848
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.0141530468237	40.70596748399856
2	18.27349093887226	21.248278972379058
3	6.036961015447746	10.529599863872006
4	2.408730313959772	5.601707287507711
5	1.1017179522958094	3.2026735446402164
6	0.5927002197816456	2.067562184867564
7	0.372187118926527	1.5147174208617438
8	0.23745121611636555	1.104426012358333
9	0.17221326827389213	0.9011173675962048
>10	0.7580462863617071	7.587901487476452
>50	0.0210085850032899	0.8258002096489291
>100	0.010641943046538205	1.1343933200794343
>500	3.413650827956738E-4	0.13042227053965294
>1k	2.675831576983316E-4	0.26428437089537477
>5k	3.5658740134241286E-5	0.13494366720526113
>10k+	5.348811020135854E-5	3.0462045360736183
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	499794	2.382807536875438	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	27365	0.1304648079940863	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.907031726571698E-5	0.0	0.0	0.0	0.0
2	1.907031726571698E-5	4.767579316429245E-6	0.0	0.0	0.0
3	1.907031726571698E-5	9.53515863285849E-6	0.0	9.53515863285849E-6	0.0
4	2.8605475898575472E-5	9.53515863285849E-6	0.0	1.4302737949287736E-5	4.767579316429245E-6
5	2.8605475898575472E-5	9.53515863285849E-6	0.0	1.907031726571698E-5	4.767579316429245E-6
6	3.3373055215004715E-5	3.3373055215004715E-5	0.0	2.8605475898575472E-5	9.53515863285849E-6
7	3.3373055215004715E-5	3.814063453143396E-5	0.0	3.814063453143396E-5	1.4302737949287736E-5
8	3.3373055215004715E-5	3.814063453143396E-5	0.0	4.290821384786321E-5	1.4302737949287736E-5
9	3.3373055215004715E-5	4.767579316429245E-5	0.0	4.290821384786321E-5	1.4302737949287736E-5
10	3.3373055215004715E-5	6.19785311135802E-5	0.0	4.290821384786321E-5	1.4302737949287736E-5
11	3.3373055215004715E-5	6.19785311135802E-5	0.0	6.674611043000943E-5	2.3837896582146226E-5
12	3.814063453143396E-5	7.151368974643868E-5	0.0	6.674611043000943E-5	3.3373055215004715E-5
13	3.814063453143396E-5	7.628126906286792E-5	0.0	6.674611043000943E-5	3.3373055215004715E-5
14	3.814063453143396E-5	7.628126906286792E-5	0.0	8.104884837929717E-5	3.3373055215004715E-5
15	3.814063453143396E-5	7.628126906286792E-5	0.0	9.53515863285849E-5	3.3373055215004715E-5
16	3.814063453143396E-5	7.628126906286792E-5	0.0	9.53515863285849E-5	3.3373055215004715E-5
17	4.767579316429245E-5	7.628126906286792E-5	0.0	9.53515863285849E-5	3.3373055215004715E-5
18	6.19785311135802E-5	7.628126906286792E-5	0.0	9.53515863285849E-5	3.814063453143396E-5
19	6.674611043000943E-5	7.628126906286792E-5	0.0	9.53515863285849E-5	4.767579316429245E-5
20	7.151368974643868E-5	8.104884837929717E-5	0.0	1.0488674496144339E-4	5.2443372480721694E-5
21	8.104884837929717E-5	8.104884837929717E-5	0.0	1.1442190359430189E-4	5.2443372480721694E-5
22	1.0488674496144339E-4	8.581642769572642E-5	0.0	1.287246415435896E-4	6.674611043000943E-5
23	1.1442190359430189E-4	8.581642769572642E-5	0.0	1.287246415435896E-4	6.674611043000943E-5
24	1.1918948291073112E-4	8.581642769572642E-5	0.0	1.3349222086001886E-4	6.674611043000943E-5
25	1.3349222086001886E-4	1.0011916564501415E-4	0.0	1.3349222086001886E-4	7.151368974643868E-5
26	1.7640043470788208E-4	1.0011916564501415E-4	0.0	1.477949588093066E-4	7.151368974643868E-5
27	2.3361138650503303E-4	1.0011916564501415E-4	0.0	1.6209769675859433E-4	7.151368974643868E-5
28	2.860547589857547E-4	1.0488674496144339E-4	0.0	1.6686527607502358E-4	7.628126906286792E-5
29	3.575684487321934E-4	1.0488674496144339E-4	0.0	1.6686527607502358E-4	8.581642769572642E-5
30	4.004766625800566E-4	1.0488674496144339E-4	0.0	1.811680140243113E-4	1.0011916564501415E-4
31	4.910606695922123E-4	1.0488674496144339E-4	0.0	1.907031726571698E-4	1.0011916564501415E-4
32	6.483907870343773E-4	1.0965432427787264E-4	0.0	2.002383312900283E-4	1.3349222086001886E-4
33	8.152560631094009E-4	1.0965432427787264E-4	0.0	2.0500591060645755E-4	1.4302737949287736E-4
34	9.582834426022783E-4	1.0965432427787264E-4	0.0	2.1454106923931603E-4	1.4302737949287736E-4
35	0.0011156135600444434	1.1918948291073112E-4	0.0	2.2407622787217453E-4	1.6209769675859433E-4
36	0.0014112034776630567	1.1918948291073112E-4	0.0	2.2407622787217453E-4	1.6209769675859433E-4
37	0.001711560974598099	1.1918948291073112E-4	0.0	2.2407622787217453E-4	1.7163285539145283E-4
38	0.002140643113076731	1.1918948291073112E-4	0.0	2.2407622787217453E-4	1.811680140243113E-4
39	0.0028700827484904054	1.1918948291073112E-4	0.0	2.765196003528962E-4	1.8593559334074055E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	134515	0.0	42.01594	12
CAACGCA	134640	0.0	41.990307	13
AAGCAGT	137180	0.0	41.980427	1
ATCAACG	138805	0.0	41.920143	11
ACGCAGA	134645	0.0	41.808273	15
AACGCAG	135505	0.0	41.76211	14
CGCAGAG	131100	0.0	41.529747	16
GTGGTAT	138785	0.0	41.503044	6
GTATCAA	139425	0.0	41.46584	9
GGTATCA	139540	0.0	41.421993	8
TGGTATC	139070	0.0	41.362984	7
AGTGGTA	138875	0.0	41.344913	5
TATCAAC	140935	0.0	41.22911	10
AGAGTAC	130345	0.0	41.166138	19
GAGTACT	69250	0.0	40.58123	20
AGCAGTG	142185	0.0	40.44889	2
CAGAGTA	133485	0.0	40.357906	18
GCAGAGT	134530	0.0	40.240093	17
CAGTGGT	143140	0.0	40.1633	4
GCAGTGG	145365	0.0	39.601177	3
>>END_MODULE