FastQCFastQC Report
Thu 26 May 2016
SRR805435_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805435_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24486017
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT526390.21497575534640853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT273050.029.2446441
CAACGCA294800.026.77408613
TCAACGC295400.026.74255212
AACGCAG304600.025.9200614
ATCAACG315750.025.73159211
ACGCAGA307200.025.58349415
GTATCAA317950.025.3554029
GGTATCA319200.025.2843048
AGTGGTA327000.024.543585
TGGTATC329450.024.3610557
TATCAAC333250.024.34658610
GTGGTAT331350.024.262116
CGCAGAG327700.024.01739316
AGAGTAC330900.023.85992819
AGCAGTG363750.022.0515462
CAGTGGT370200.021.807134
CAGAGTA358400.021.73409718
GCAGTGG374600.021.5509873
GCAGAGT375650.020.92173617
GAGTACT147400.020.24084320
Produced by FastQC (version 0.11.5)