##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805435_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24486017
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.48330714627863	37.0	37.0	37.0	35.0	37.0
2	36.12386032403718	37.0	37.0	37.0	35.0	37.0
3	38.07449553759601	39.0	39.0	39.0	37.0	39.0
4	38.101318193154896	39.0	39.0	39.0	37.0	39.0
5	38.06781858396978	39.0	39.0	39.0	37.0	39.0
6	38.095556006515885	39.0	39.0	39.0	37.0	39.0
7	37.99352814302138	39.0	39.0	39.0	35.0	39.0
8	39.001495424919455	40.0	40.0	40.0	37.0	40.0
9	38.85979577650379	40.0	40.0	40.0	37.0	40.0
10	38.978665987204046	40.0	40.0	40.0	37.0	40.0
11	39.015300405941886	40.0	40.0	40.0	37.0	40.0
12	38.952261611188135	40.0	40.0	40.0	37.0	40.0
13	38.995154581490326	40.0	40.0	40.0	37.0	40.0
14	38.98997080660362	40.0	40.0	40.0	37.0	40.0
15	38.94960879100917	40.0	40.0	40.0	37.0	40.0
16	38.906352184595804	40.0	40.0	40.0	37.0	40.0
17	38.90423893767614	40.0	40.0	40.0	37.0	40.0
18	38.2484879022995	40.0	39.0	40.0	35.0	40.0
19	38.600880126808704	40.0	39.0	40.0	36.0	40.0
20	38.63245038995113	40.0	39.0	40.0	36.0	40.0
21	38.53788927778658	40.0	39.0	40.0	36.0	40.0
22	38.46384701930085	40.0	39.0	40.0	36.0	40.0
23	38.32816978767923	40.0	39.0	40.0	35.0	40.0
24	38.44569012591962	40.0	39.0	40.0	35.0	40.0
25	38.21985021083666	40.0	38.0	40.0	35.0	40.0
26	38.07972701317654	40.0	38.0	40.0	34.0	40.0
27	38.35023066430118	40.0	39.0	40.0	35.0	40.0
28	38.50644088011537	40.0	39.0	40.0	35.0	40.0
29	38.5311686665904	40.0	39.0	40.0	36.0	40.0
30	38.43159922661166	40.0	39.0	40.0	35.0	40.0
31	38.50163858826039	40.0	39.0	40.0	35.0	40.0
32	38.49847082112211	40.0	39.0	40.0	35.0	40.0
33	38.38373374485528	40.0	39.0	40.0	35.0	40.0
34	38.4306903405319	40.0	39.0	40.0	35.0	40.0
35	38.406124809927235	40.0	39.0	40.0	35.0	40.0
36	38.35722057205139	40.0	39.0	40.0	35.0	40.0
37	38.30615477396753	40.0	39.0	40.0	35.0	40.0
38	38.26743675788512	40.0	39.0	40.0	35.0	40.0
39	37.71221763016827	40.0	38.0	40.0	33.0	40.0
40	37.91905396455454	40.0	38.0	40.0	34.0	40.0
41	37.977606280351765	40.0	38.0	40.0	34.0	40.0
42	37.432601757974766	40.0	38.0	40.0	33.0	40.0
43	37.68817117132607	40.0	38.0	40.0	33.0	40.0
44	37.76990381898371	40.0	38.0	40.0	33.0	40.0
45	37.73466619744649	40.0	38.0	40.0	33.0	40.0
46	37.715875432088446	40.0	38.0	40.0	33.0	40.0
47	37.63468725844632	40.0	37.0	40.0	33.0	40.0
48	37.403492899641456	40.0	37.0	40.0	33.0	40.0
49	37.3470188311966	40.0	37.0	40.0	33.0	40.0
50	36.78910869824194	40.0	36.0	40.0	31.0	40.0
51	36.52983737616453	39.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	90.0
9	230.0
10	349.0
11	448.0
12	484.0
13	478.0
14	537.0
15	648.0
16	966.0
17	1796.0
18	3426.0
19	6218.0
20	10988.0
21	17718.0
22	26521.0
23	37240.0
24	49553.0
25	64742.0
26	82654.0
27	103873.0
28	128557.0
29	158937.0
30	195152.0
31	241097.0
32	298984.0
33	379994.0
34	507333.0
35	726760.0
36	1116833.0
37	1900321.0
38	3979153.0
39	1.4443933E7
>>END_MODULE
>>Per base sequence content	pass
#Base	G	A	T	C
1	22.270359446373007	29.106326275931277	20.8336741741215	27.789640103574214
2	25.20529165686686	23.460091528973454	29.01053691174028	22.32407990241941
3	21.019392414862736	25.481633047955494	27.065177648124646	26.43379688905713
4	21.9217931605618	27.884890384581535	26.51790203363822	23.675414421218445
5	25.660081833644078	27.36689678848136	25.105573519776613	21.86744785809795
6	24.59012831690838	25.584989996535572	26.921107667286194	22.903774019269854
7	24.341035130376657	26.400234876909543	26.75368558308197	22.505044409631832
8	23.215617305174625	25.476046185870082	27.392499155742644	23.915837353212652
9	23.17055893573871	25.1986960557938	28.18979501647818	23.440949991989303
10	23.059687494295215	26.15293863432342	27.421250258872238	23.36612361250913
11	23.00580776367181	26.80686695594469	26.819212777643664	23.36811250273983
12	23.105170595936446	25.93639055302461	27.192875019240574	23.76556383179837
13	23.39469910520768	26.284650541572358	26.09340261423489	24.22724773898507
14	23.46339137149174	26.892221793360676	26.12970904986303	23.514677785284558
15	23.44549544337897	26.613687313865707	26.07590691454637	23.864910328208953
16	23.890974183347176	25.996931228137264	25.851713653551737	24.26038093496382
17	24.564436102449818	25.90261209081085	25.896290115293148	23.636661691446186
18	23.779657589880788	26.109534270110164	25.684777561005532	24.42603057900352
19	24.001310625570504	26.612568307863217	25.723665061573715	23.662456004992563
20	24.489323845523753	25.942418483169394	25.971880195950202	23.59637747535665
21	24.029261271851603	26.355470552846548	25.962152194862888	23.653115980438958
22	24.577075969521708	25.83559833353052	25.96125372289009	23.626071974057684
23	23.727088811544974	25.986835670333807	26.766170259540374	23.51990525858085
24	23.602642275385172	26.460105782006117	26.001382748366137	23.935869194242578
25	23.692877449198864	26.1116007556476	25.83877157317991	24.356750221973627
26	23.9356200724683	26.06376529102303	26.526216983350132	23.474397653158537
27	23.84348585562119	25.61531342561757	26.755756152582922	23.78544456617832
28	24.37098283481548	25.820957324337396	26.095154634581853	23.712905206265276
29	24.362185977409066	25.795959383676	26.271851399923474	23.570003238991465
30	24.344383980457092	25.601648483703986	26.26871083198219	23.785256703856735
31	24.172657398710456	25.906998267623514	26.050386226555343	23.869958107110683
32	24.03145436025794	26.018690585733072	26.204792719044505	23.74506233496448
33	24.085297335209724	25.724927006299147	26.24645731480134	23.943318343689786
34	24.074152198783494	25.947895078239963	26.04422352561464	23.933729197361906
35	24.008551492878567	25.960784067086124	26.183556108778326	23.847108331256976
36	23.91923521085524	25.85473578655116	26.17482459478812	24.051204407805482
37	24.1265576185788	25.96044509811457	25.966101387579695	23.946895895726936
38	24.08778038502546	25.99510569644708	26.146878032470532	23.770235886056927
39	24.04691624611712	26.06168655359506	26.049671532940614	23.841725667347205
40	24.120950336675826	25.98120796861327	25.958497047518996	23.939344647191906
41	24.02281677742852	25.986688647647348	26.20406577353924	23.786428801384886
42	24.02536517066046	25.970949052269297	26.12948443186983	23.874201345200404
43	24.14052068982881	25.92845132795587	25.950929463129917	23.980098519085402
44	24.092321752451614	25.88143673999736	26.186978470201993	23.83926303734903
45	24.06080172206039	25.680840620179264	26.238019846184045	24.020337811576297
46	24.11202687640052	25.911903107802303	25.989183949353624	23.986886066443557
47	24.107718294894593	25.892059129093965	26.15568714176748	23.84453543424396
48	24.215837144930514	25.669050217518024	26.174203832334186	23.94090880521728
49	24.149701439805423	25.973967101305206	25.910212346908036	23.96611911198134
50	24.04114968963715	25.936958223952878	26.26727327682571	23.75461880958426
51	24.185313601636395	25.726842385186615	26.118339295443597	23.96950471773339
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7350.0
1	4814.5
2	2279.0
3	2010.0
4	1741.0
5	1884.5
6	2028.0
7	2355.5
8	2683.0
9	3351.0
10	4019.0
11	5917.5
12	7816.0
13	10691.5
14	13567.0
15	17956.5
16	22346.0
17	31349.5
18	40353.0
19	54828.5
20	69304.0
21	93088.0
22	116872.0
23	179948.0
24	243024.0
25	247819.0
26	294335.0
27	336056.0
28	381541.5
29	427027.0
30	481591.0
31	536155.0
32	597134.5
33	658114.0
34	732060.0
35	806006.0
36	896853.5
37	987701.0
38	1065675.0
39	1143649.0
40	1229858.0
41	1316067.0
42	1392292.5
43	1468518.0
44	1543764.5
45	1619011.0
46	1674168.5
47	1729326.0
48	1759025.5
49	1788725.0
50	1809931.0
51	1831137.0
52	1814488.0
53	1797839.0
54	1731368.5
55	1664898.0
56	1562430.5
57	1459963.0
58	1344914.5
59	1229866.0
60	1099298.0
61	968730.0
62	842492.0
63	716254.0
64	609691.0
65	503128.0
66	405027.0
67	306926.0
68	252296.5
69	197667.0
70	150172.0
71	102677.0
72	76229.5
73	49782.0
74	38075.0
75	20307.5
76	14247.0
77	10580.0
78	6913.0
79	5280.5
80	3648.0
81	2814.0
82	1980.0
83	1444.0
84	908.0
85	637.5
86	367.0
87	280.5
88	194.0
89	136.5
90	79.0
91	61.0
92	43.0
93	40.0
94	37.0
95	23.0
96	9.0
97	6.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.4486017E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.179682813699486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.21931106486977	31.041664639310863
2	17.105629121500765	15.798650543193727
3	6.442501604239094	8.925380418315342
4	2.9782350556448836	5.501358008572854
5	1.5927084707345853	3.6775385996607763
6	0.9557876516978564	2.648278235559461
7	0.6368389151179977	2.0586313372498735
8	0.4798065371045749	1.772585095634304
9	0.331419435818387	1.377435998194951
>10	2.0646705785286104	18.095553614705597
>50	0.14290314073864313	4.4816863316941955
>100	0.04806909811856015	3.607353846289681
>500	0.0019267745206060492	0.5901572063256946
>1k	1.6516936348350377E-4	0.12720270982191464
>5k	9.127334069907634E-6	0.02626771748707702
>10k+	1.8254668139790576E-5	0.2702556979836531
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	52639	0.21497575534640853	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.940453729163057E-4	0.0	0.0	4.083963512726467E-6	1.633585405090587E-5
2	2.9812933642903215E-4	0.0	0.0	8.167927025452934E-6	2.041981756363234E-5
3	3.022132999417586E-4	4.083963512726467E-6	0.0	1.633585405090587E-5	3.675567161453821E-5
4	3.1038122696721155E-4	4.083963512726467E-6	0.0	2.041981756363234E-5	4.4923598639991145E-5
5	3.1038122696721155E-4	4.083963512726467E-6	0.0	2.4503781076358806E-5	5.309152566544408E-5
6	3.1038122696721155E-4	4.083963512726467E-6	0.0	2.4503781076358806E-5	6.1259452690897E-5
7	3.14465190479938E-4	4.083963512726467E-6	0.0	3.675567161453821E-5	6.1259452690897E-5
8	3.14465190479938E-4	4.083963512726467E-6	0.0	3.675567161453821E-5	7.759530674180289E-5
9	3.14465190479938E-4	4.083963512726467E-6	0.0	4.900756215271761E-5	9.393116079270876E-5
10	3.14465190479938E-4	4.083963512726467E-6	0.0	4.900756215271761E-5	1.1843494186906756E-4
11	3.14465190479938E-4	4.083963512726467E-6	0.0	4.900756215271761E-5	1.22518905381794E-4
12	3.185491539926645E-4	4.083963512726467E-6	0.0	5.7175489178170546E-5	1.511066499708793E-4
13	3.185491539926645E-4	4.083963512726467E-6	0.0	6.1259452690897E-5	1.6744250402178518E-4
14	3.185491539926645E-4	8.167927025452934E-6	0.0	6.534341620362347E-5	1.7152646753451165E-4
15	3.2263311750539096E-4	8.167927025452934E-6	0.0	7.351134322907642E-5	1.7152646753451165E-4
16	3.267170810181174E-4	8.167927025452934E-6	0.0	7.351134322907642E-5	1.7969439455996458E-4
17	3.308010445308439E-4	8.167927025452934E-6	0.0	7.759530674180289E-5	1.9194628509814398E-4
18	3.308010445308439E-4	8.167927025452934E-6	0.0	7.759530674180289E-5	1.9603024861087045E-4
19	3.430529350690233E-4	8.167927025452934E-6	0.0	8.167927025452936E-5	2.0828213914904985E-4
20	3.634727526326556E-4	8.167927025452934E-6	0.0	8.984719727998229E-5	2.1236610266177632E-4
21	3.7164067965810856E-4	8.167927025452934E-6	0.0	9.393116079270876E-5	2.1645006617450278E-4
22	3.8797653370901443E-4	8.167927025452934E-6	0.0	9.393116079270876E-5	2.3686988373813512E-4
23	4.2064824181082617E-4	8.167927025452934E-6	0.0	9.801512430543522E-5	2.5728970130176743E-4
24	4.4923598639991144E-4	8.167927025452934E-6	0.0	1.0209908781816169E-4	2.6545762832722036E-4
25	4.6965580396354377E-4	8.167927025452934E-6	0.0	1.0209908781816169E-4	2.777095188653998E-4
26	5.104954390908084E-4	8.167927025452934E-6	0.0	1.0209908781816169E-4	2.777095188653998E-4
27	5.268312931417143E-4	8.167927025452934E-6	0.0	1.1435097835634109E-4	2.8587744589085275E-4
28	5.717548917817055E-4	8.167927025452934E-6	0.0	1.1435097835634109E-4	2.899614094035792E-4
29	6.085105633962437E-4	8.167927025452934E-6	0.0	1.1843494186906756E-4	2.899614094035792E-4
30	6.37098307985329E-4	8.167927025452934E-6	0.0	1.1843494186906756E-4	2.9812933642903215E-4
31	6.820219066253201E-4	8.167927025452934E-6	0.0	1.2660286889452048E-4	3.14465190479938E-4
32	8.372125201089258E-4	8.167927025452934E-6	0.0	1.3068683240724695E-4	3.2263311750539096E-4
33	9.107238633380022E-4	8.167927025452934E-6	0.0	1.388547594326999E-4	3.3488500804357036E-4
34	9.760672795416257E-4	8.167927025452934E-6	0.0	1.388547594326999E-4	3.430529350690233E-4
35	0.0010250748416943433	1.2251890538179403E-5	0.0	1.4293872294542638E-4	3.675567161453821E-4
36	0.0011108380754615993	1.2251890538179403E-5	0.0	1.4702268645815284E-4	3.798086066835615E-4
37	0.0011925173457161286	1.2251890538179403E-5	0.0	1.4702268645815284E-4	3.8389257019628796E-4
38	0.001302784360559743	1.2251890538179403E-5	0.0	1.4702268645815284E-4	3.920604972217409E-4
39	0.0014130513754033577	1.2251890538179403E-5	0.0	1.511066499708793E-4	3.9614446073446736E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	27305	0.0	29.244644	1
CAACGCA	29480	0.0	26.774086	13
TCAACGC	29540	0.0	26.742552	12
AACGCAG	30460	0.0	25.92006	14
ATCAACG	31575	0.0	25.731592	11
ACGCAGA	30720	0.0	25.583494	15
GTATCAA	31795	0.0	25.355402	9
GGTATCA	31920	0.0	25.284304	8
AGTGGTA	32700	0.0	24.54358	5
TGGTATC	32945	0.0	24.361055	7
TATCAAC	33325	0.0	24.346586	10
GTGGTAT	33135	0.0	24.26211	6
CGCAGAG	32770	0.0	24.017393	16
AGAGTAC	33090	0.0	23.859928	19
AGCAGTG	36375	0.0	22.051546	2
CAGTGGT	37020	0.0	21.80713	4
CAGAGTA	35840	0.0	21.734097	18
GCAGTGG	37460	0.0	21.550987	3
GCAGAGT	37565	0.0	20.921736	17
GAGTACT	14740	0.0	20.240843	20
>>END_MODULE