FastQCFastQC Report
Thu 26 May 2016
SRR805434_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805434_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19346489
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT857630.4433000737239713No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT373350.032.5612681
CAACGCA384350.031.25471313
TCAACGC385150.031.19563512
ATCAACG399850.030.87032511
AACGCAG390800.030.78492514
ACGCAGA389250.030.78034415
GTGGTAT406850.030.1235086
GGTATCA409100.030.0073328
AGTGGTA408400.029.926545
GTATCAA411900.029.7978869
TGGTATC412700.029.669257
AGAGTAC373850.029.55062119
TATCAAC421750.029.3473610
CGCAGAG388650.029.21265616
AGCAGTG443100.027.6235622
GCAGTGG445650.027.5058883
CAGTGGT447200.027.4508044
CAGAGTA412800.027.28015918
GCAGAGT418900.027.06552717
GAGTACT182100.026.71334520
Produced by FastQC (version 0.11.5)