##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805434_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19346489
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.270529603588535	37.0	37.0	37.0	35.0	37.0
2	35.97293612293166	37.0	37.0	37.0	35.0	37.0
3	38.0156213874259	39.0	39.0	39.0	35.0	39.0
4	38.08323923787928	39.0	39.0	39.0	37.0	39.0
5	38.0500145530282	39.0	39.0	39.0	37.0	39.0
6	38.08178465870474	39.0	39.0	39.0	37.0	39.0
7	38.0038329952272	39.0	39.0	39.0	35.0	39.0
8	38.98817635592691	40.0	40.0	40.0	37.0	40.0
9	38.80805995341067	40.0	40.0	40.0	36.0	40.0
10	38.92756137819116	40.0	40.0	40.0	37.0	40.0
11	38.977966079529985	40.0	40.0	40.0	37.0	40.0
12	38.92050960771228	40.0	40.0	40.0	37.0	40.0
13	38.966004580986244	40.0	40.0	40.0	37.0	40.0
14	38.96327638570492	40.0	40.0	40.0	37.0	40.0
15	38.92910677487786	40.0	40.0	40.0	37.0	40.0
16	38.86908265370528	40.0	39.0	40.0	37.0	40.0
17	38.877306678229836	40.0	39.0	40.0	37.0	40.0
18	38.03240789582027	40.0	39.0	40.0	34.0	40.0
19	38.49727710283763	40.0	39.0	40.0	35.0	40.0
20	38.5702457432974	40.0	39.0	40.0	36.0	40.0
21	38.507873082294154	40.0	39.0	40.0	36.0	40.0
22	36.221392780881324	40.0	38.0	40.0	27.0	40.0
23	36.676395701566314	40.0	38.0	40.0	27.0	40.0
24	37.773161683238754	40.0	38.0	40.0	32.0	40.0
25	35.49125492486001	40.0	38.0	40.0	24.0	40.0
26	36.28579640471199	40.0	38.0	40.0	19.0	40.0
27	37.41897550506451	40.0	38.0	40.0	30.0	40.0
28	38.11901523837219	40.0	38.0	40.0	34.0	40.0
29	38.356271672860125	40.0	39.0	40.0	35.0	40.0
30	38.37250872755258	40.0	39.0	40.0	35.0	40.0
31	38.45661148128738	40.0	39.0	40.0	35.0	40.0
32	38.46492658176892	40.0	39.0	40.0	35.0	40.0
33	38.295355865345904	40.0	39.0	40.0	35.0	40.0
34	38.36949458891482	40.0	39.0	40.0	35.0	40.0
35	38.359186878818164	40.0	39.0	40.0	35.0	40.0
36	38.2989267458297	40.0	39.0	40.0	35.0	40.0
37	38.240187302202486	40.0	39.0	40.0	35.0	40.0
38	38.19462911332387	40.0	38.0	40.0	35.0	40.0
39	37.5619316249062	40.0	38.0	40.0	33.0	40.0
40	37.81559951265576	40.0	38.0	40.0	33.0	40.0
41	37.88431802793778	40.0	38.0	40.0	34.0	40.0
42	37.056581067500154	40.0	38.0	40.0	31.0	40.0
43	37.458255758964846	40.0	38.0	40.0	33.0	40.0
44	37.641585612769326	40.0	38.0	40.0	33.0	40.0
45	37.65136278732539	40.0	38.0	40.0	33.0	40.0
46	37.65810395881134	40.0	38.0	40.0	33.0	40.0
47	37.57061800722602	40.0	38.0	40.0	33.0	40.0
48	37.34570200308697	40.0	37.0	40.0	33.0	40.0
49	37.295195577864284	40.0	37.0	40.0	33.0	40.0
50	36.48014339966285	40.0	36.0	40.0	30.0	40.0
51	36.38239677493937	39.0	35.0	40.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	39.0
9	114.0
10	168.0
11	215.0
12	210.0
13	272.0
14	330.0
15	461.0
16	787.0
17	1467.0
18	2882.0
19	5208.0
20	9355.0
21	15201.0
22	22969.0
23	32623.0
24	43422.0
25	56480.0
26	72625.0
27	89463.0
28	111950.0
29	139191.0
30	172093.0
31	215917.0
32	279829.0
33	375871.0
34	538868.0
35	841660.0
36	1361088.0
37	1727427.0
38	2911893.0
39	1.0316406E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.219236368934954	29.591612204157556	22.072387398044164	34.11676402886332
2	22.85182081358535	25.400071299758835	30.661160275644843	21.08694761101097
3	17.75075053669945	25.53893887412853	31.081262341709653	25.62904824746237
4	18.1671258283609	27.139596233714553	30.93601634901299	23.75726158891156
5	23.39653463737012	28.046442948898893	28.719645202806564	19.837377210924423
6	23.007347741494595	26.331857940735397	29.08684361281264	21.57395070495737
7	23.453030676522236	27.103620713815307	28.89999834078421	20.543350268878243
8	22.143547596672448	25.589438993297442	29.282822325022384	22.984191085007723
9	22.217281905776286	24.918366324763113	30.636923319781694	22.227428449678904
10	22.103798782301016	26.153112329580836	29.026853399601343	22.716235488516805
11	21.866499911172514	27.81996774711939	27.733357716741263	22.580174624966833
12	22.09672773183806	26.45739493093553	28.66084383579884	22.78503350142757
13	22.372374646376407	26.701532252182815	26.82463986101044	24.10145324043034
14	22.598948057190118	28.093759027800857	26.694993598063192	22.61229931694583
15	22.385271043236838	27.893546989327106	26.78066805816807	22.94051390926798
16	22.94782272897165	26.514624953395938	26.486459636164472	24.05109268146794
17	24.39091661541275	26.3240735825503	26.488444492434777	22.796565309602173
18	22.95084653344594	26.274989741032595	26.20067134662005	24.573492378901413
19	23.090225828572823	27.613801139834727	26.358095259558468	22.937877772033985
20	24.297990193466113	26.451455868814232	26.428790257498402	22.821763680221256
21	23.22477220543738	27.518698612445906	26.548248625370736	22.70828055674598
22	24.954724343006113	26.929465082785818	26.808192432228918	21.307618141979145
23	22.816108907409504	26.463825038227867	27.98773462202884	22.732331432333794
24	22.62602790614876	27.60880281688321	26.68099105734379	23.084178219624242
25	20.95309903517894	31.99346920260312	24.554465670747803	22.498966091470137
26	23.01075404431264	26.856221818853022	27.485250682953378	22.64777345388096
27	22.914090510169572	26.077207083931352	28.14669886613535	22.862003539763727
28	23.78034071195037	26.097071153324	27.086444470622034	23.0361436641036
29	23.787215344344908	26.188663999964024	27.16925019314874	22.854870462542326
30	23.73605877531577	26.035499257772305	27.262512593370303	22.965929373541627
31	23.477159085558107	26.224686039932106	27.078246600713957	23.21990827379583
32	23.400680092392992	26.486666392025963	27.130142321947925	22.98251119363312
33	23.408014756579345	26.27109756194005	27.252024902296224	23.06886277918438
34	23.28049291010891	26.36257669285626	27.055007241882496	23.301923155152338
35	23.234370846307048	26.497479723581886	27.17565445595839	23.092494974152675
36	23.160321234514438	26.385914260721933	27.223270330859517	23.230494173904113
37	23.32004013751539	26.38518544631018	27.012803201655867	23.281971214518563
38	23.39848331136466	26.48573599064926	27.151458851267535	22.964321846718544
39	23.358160749477594	26.55700473610483	27.073501553692765	23.01133296072481
40	23.324609442054317	26.44872152254603	26.95961008739105	23.267058948008604
41	23.28883034022349	26.565719495666627	27.13265440566503	23.012795758444852
42	23.355757212587772	26.385340513206295	27.145209655354	23.11369261885193
43	23.24775311944198	26.43101805190596	26.99972589341663	23.321502935235433
44	23.3329055209966	26.426288511574374	27.155960960151475	23.08484500727755
45	23.33537108464487	26.238993545547206	27.22172483079488	23.203910539013048
46	23.24304425469655	26.369156698148178	26.986173046695967	23.40162600045931
47	23.331752857068793	26.428914311015294	27.080572604155723	23.15876022776019
48	23.38460999305869	26.228671259162322	27.19319252190927	23.193526225869718
49	23.305815334244887	26.426815739021176	26.867479675511145	23.399889251222795
50	23.243747224625615	26.43630066416702	27.298539802234917	23.021412308972444
51	23.48226078644037	26.267065822640994	27.05591696767305	23.194756423245583
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11466.0
1	7917.0
2	4368.0
3	3693.0
4	3018.0
5	3314.5
6	3611.0
7	4278.5
8	4946.0
9	6181.5
10	7417.0
11	10554.0
12	13691.0
13	18060.0
14	22429.0
15	29318.5
16	36208.0
17	47829.0
18	59450.0
19	79184.5
20	98919.0
21	126460.0
22	154001.0
23	237540.0
24	321079.0
25	309694.5
26	333695.0
27	369080.0
28	408680.5
29	448281.0
30	494135.5
31	539990.0
32	590989.0
33	641988.0
34	695351.5
35	748715.0
36	807893.0
37	867071.0
38	917218.0
39	967365.0
40	1023942.5
41	1080520.0
42	1132394.0
43	1184268.0
44	1234719.0
45	1285170.0
46	1323143.5
47	1361117.0
48	1370343.5
49	1379570.0
50	1372613.5
51	1365657.0
52	1331217.5
53	1296778.0
54	1234616.0
55	1172454.0
56	1085844.5
57	999235.0
58	906162.5
59	813090.0
60	715453.0
61	617816.0
62	530937.5
63	444059.0
64	373653.5
65	303248.0
66	243518.0
67	183788.0
68	148650.5
69	113513.0
70	87570.0
71	61627.0
72	46159.5
73	30692.0
74	22965.0
75	11815.0
76	8392.0
77	6378.0
78	4364.0
79	3292.0
80	2220.0
81	1737.0
82	1254.0
83	914.0
84	574.0
85	400.5
86	227.0
87	168.5
88	110.0
89	84.5
90	59.0
91	43.0
92	27.0
93	19.0
94	11.0
95	8.0
96	5.0
97	3.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.9346489E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.00031715327016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.74549615990025	31.372947079343067
2	20.091580888887297	20.09170833109893
3	7.868504531095589	11.802831662301678
4	3.4894392506666794	6.978922768816133
5	1.7770769650042273	4.442720592799106
6	1.061674776213286	3.1850445314574856
7	0.6365205883637106	2.2278361904940156
8	0.44199626643937684	1.7679962802024112
9	0.32371227293273336	1.456714468173836
>10	1.5025266638561519	12.801956392554601
>50	0.04465678957691543	1.4920164387500372
>100	0.01606550337275519	1.4431395377415996
>500	5.971517260966356E-4	0.18671779015626588
>1k	1.087340465008159E-4	0.11810358796620728
>5k	1.0864479629526684E-5	0.03332157038331795
>10k+	3.259343888858001E-5	0.5980227777613821
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	85763	0.4433000737239713	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.820903420770559E-5	0.0	0.0	0.0	4.13511722979813E-5
2	9.820903420770559E-5	0.0	0.0	1.550668961174299E-5	4.652006883522897E-5
3	9.820903420770559E-5	0.0	0.0	2.067558614899065E-5	6.719565498421962E-5
4	9.820903420770559E-5	0.0	0.0	4.652006883522897E-5	1.0854682728220091E-4
5	4.3935620566605137E-4	0.0	0.0	4.652006883522897E-5	1.1371572381944858E-4
6	4.44525102203299E-4	0.0	0.0	4.652006883522897E-5	1.4472910304293455E-4
7	4.60031791815042E-4	0.0	0.0	5.168896537247663E-5	1.550668961174299E-4
8	4.60031791815042E-4	0.0	0.0	6.202675844697195E-5	1.654046891919252E-4
9	4.6520068835228964E-4	0.0	0.0	7.236455152146728E-5	1.7057358572917288E-4
10	4.8070737796403266E-4	0.0	0.0	7.236455152146728E-5	1.9124917187816353E-4
11	4.8070737796403266E-4	0.0	0.0	7.753344805871494E-5	2.222625511016495E-4
12	6.150986879324719E-4	0.0	0.0	7.753344805871494E-5	2.791204130113738E-4
13	7.236455152146728E-4	0.0	0.0	9.820903420770559E-5	2.9979599916036447E-4
14	8.011789632733878E-4	0.0	0.0	1.2922241343119156E-4	3.2564048184660274E-4
15	8.993879974810934E-4	0.0	0.0	1.4472910304293455E-4	3.4114717145834576E-4
16	9.5107696285357E-4	0.0	0.0	1.7057358572917288E-4	3.9283613683082237E-4
17	0.0010182726178377896	5.1688965372476625E-6	0.0	1.809113788036682E-4	4.3418730912880366E-4
18	0.0011113127555082475	5.1688965372476625E-6	0.0	1.9641806841541118E-4	4.44525102203299E-4
19	0.0011371572381944858	5.1688965372476625E-6	0.0	2.222625511016495E-4	4.75538481426785E-4
20	0.0011681706174179718	1.0337793074495325E-5	0.0	2.4293813725064016E-4	4.858762745012803E-4
21	0.0011940151001042102	1.0337793074495325E-5	0.0	2.481070337878878E-4	4.962140675757756E-4
22	0.0012250284793276962	1.550668961174299E-5	0.0	2.584448268623831E-4	5.323963433365093E-4
23	0.0012612107550884297	1.550668961174299E-5	0.0	2.687826199368785E-4	5.479030329482523E-4
24	0.001318068616998154	1.550668961174299E-5	0.0	2.791204130113738E-4	5.789164121717382E-4
25	0.0013852642719823737	1.550668961174299E-5	0.0	2.8428930954862145E-4	6.099297913952242E-4
26	0.0014472910304293455	1.550668961174299E-5	0.0	2.894582060858691E-4	6.254364810069672E-4
27	0.0014989799958018222	1.550668961174299E-5	0.0	3.049648956976121E-4	6.306053775442149E-4
28	0.001535162271562556	1.550668961174299E-5	0.0	3.2564048184660274E-4	6.357742740814626E-4
29	0.0016488779953820044	1.550668961174299E-5	0.0	3.4114717145834576E-4	6.512809636932055E-4
30	0.0016953980642172334	1.550668961174299E-5	0.0	3.6699165414458404E-4	6.667876533049485E-4
31	0.0017264114434407194	1.550668961174299E-5	0.0	3.7216055068183175E-4	6.771254463794438E-4
32	0.0018039448914994344	1.550668961174299E-5	0.0	3.773294472190794E-4	7.184766186774252E-4
33	0.0018763094430209017	1.550668961174299E-5	0.0	3.9800503336807E-4	7.494899979009111E-4
34	0.0019279984083933784	1.550668961174299E-5	0.0	4.2384951605430835E-4	7.649966875126541E-4
35	0.0020055318564520933	1.550668961174299E-5	0.0	4.3418730912880366E-4	8.063478598106355E-4
36	0.0020468830287500746	1.550668961174299E-5	0.0	4.60031791815042E-4	8.425301355713691E-4
37	0.0021244164768087896	1.550668961174299E-5	0.0	4.6520068835228964E-4	8.73543514794855E-4
38	0.0021761054421812662	1.550668961174299E-5	0.0	4.962140675757756E-4	8.942191009438456E-4
39	0.002243301097165486	1.550668961174299E-5	0.0	5.065518606502709E-4	9.097257905555887E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	37335	0.0	32.561268	1
CAACGCA	38435	0.0	31.254713	13
TCAACGC	38515	0.0	31.195635	12
ATCAACG	39985	0.0	30.870325	11
AACGCAG	39080	0.0	30.784925	14
ACGCAGA	38925	0.0	30.780344	15
GTGGTAT	40685	0.0	30.123508	6
GGTATCA	40910	0.0	30.007332	8
AGTGGTA	40840	0.0	29.92654	5
GTATCAA	41190	0.0	29.797886	9
TGGTATC	41270	0.0	29.66925	7
AGAGTAC	37385	0.0	29.550621	19
TATCAAC	42175	0.0	29.34736	10
CGCAGAG	38865	0.0	29.212656	16
AGCAGTG	44310	0.0	27.623562	2
GCAGTGG	44565	0.0	27.505888	3
CAGTGGT	44720	0.0	27.450804	4
CAGAGTA	41280	0.0	27.280159	18
GCAGAGT	41890	0.0	27.065527	17
GAGTACT	18210	0.0	26.713345	20
>>END_MODULE