FastQCFastQC Report
Thu 26 May 2016
SRR805433_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805433_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25536785
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4834421.8931200619028592No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC325620.12751017796484562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2156500.042.609671
TCAACGC2141100.042.1574212
CAACGCA2144850.042.1088913
ATCAACG2211150.042.03785711
GTATCAA2199200.041.9808359
GGTATCA2200300.041.956788
GTGGTAT2195350.041.9304476
AACGCAG2157550.041.89439414
ACGCAGA2147400.041.89019415
AGTGGTA2193200.041.8648575
TGGTATC2202600.041.732167
TATCAAC2227250.041.68649710
CGCAGAG2070600.041.4738516
AGAGTAC2029250.041.3543219
AGCAGTG2235150.041.145562
GCAGTGG2247050.040.9216543
CAGTGGT2247750.040.8598634
CAGAGTA2076650.040.8221218
GCAGAGT2109150.040.45978517
GTACATG1396300.040.04977422
Produced by FastQC (version 0.11.5)