##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805433_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25536785
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.329923246015504	37.0	37.0	37.0	35.0	37.0
2	36.00613413160662	37.0	37.0	37.0	35.0	37.0
3	38.01946443140748	39.0	39.0	39.0	35.0	39.0
4	38.04915493473435	39.0	39.0	39.0	35.0	39.0
5	37.99713922484761	39.0	39.0	39.0	35.0	39.0
6	38.054064988995286	39.0	39.0	39.0	35.0	39.0
7	37.87742987224116	39.0	38.0	39.0	35.0	39.0
8	38.915930372597806	40.0	39.0	40.0	37.0	40.0
9	38.770999364250436	40.0	40.0	40.0	36.0	40.0
10	38.691924884044724	40.0	39.0	40.0	36.0	40.0
11	38.867948804048744	40.0	39.0	40.0	36.0	40.0
12	38.878131370100036	40.0	39.0	40.0	37.0	40.0
13	38.94602358910881	40.0	40.0	40.0	37.0	40.0
14	38.95135844234112	40.0	40.0	40.0	37.0	40.0
15	38.91310863916503	40.0	40.0	40.0	37.0	40.0
16	38.84758351530939	40.0	39.0	40.0	36.0	40.0
17	38.84589109396504	40.0	39.0	40.0	36.0	40.0
18	38.054306092172524	40.0	39.0	40.0	34.0	40.0
19	38.49471713060199	40.0	39.0	40.0	35.0	40.0
20	38.57663331543105	40.0	39.0	40.0	36.0	40.0
21	38.421257061137496	40.0	39.0	40.0	36.0	40.0
22	36.32380986878341	40.0	38.0	40.0	27.0	40.0
23	36.51432930966055	40.0	37.0	40.0	26.0	40.0
24	37.673731755974764	40.0	38.0	40.0	32.0	40.0
25	35.59601629570833	40.0	38.0	40.0	25.0	40.0
26	36.36711441945413	40.0	38.0	40.0	24.0	40.0
27	37.449094668729835	40.0	38.0	40.0	30.0	40.0
28	38.11867981815252	40.0	38.0	40.0	34.0	40.0
29	38.32974883095112	40.0	39.0	40.0	35.0	40.0
30	38.32607636395889	40.0	39.0	40.0	35.0	40.0
31	38.39944452678753	40.0	39.0	40.0	35.0	40.0
32	38.40612402853374	40.0	39.0	40.0	35.0	40.0
33	38.22161062952913	40.0	39.0	40.0	35.0	40.0
34	38.28920472956952	40.0	39.0	40.0	35.0	40.0
35	38.27589228636259	40.0	39.0	40.0	35.0	40.0
36	38.207455793671755	40.0	39.0	40.0	35.0	40.0
37	38.10960244212418	40.0	38.0	40.0	35.0	40.0
38	38.02415777867104	40.0	38.0	40.0	34.0	40.0
39	37.41479735996524	40.0	38.0	40.0	33.0	40.0
40	37.645874803739	40.0	38.0	40.0	33.0	40.0
41	37.70671817145345	40.0	38.0	40.0	33.0	40.0
42	36.906622936285835	40.0	37.0	40.0	31.0	40.0
43	37.270228613351286	40.0	37.0	40.0	32.0	40.0
44	37.43407394470369	40.0	37.0	40.0	33.0	40.0
45	37.42945593973556	40.0	37.0	40.0	33.0	40.0
46	37.4128505604758	40.0	37.0	40.0	33.0	40.0
47	37.31696441035941	40.0	37.0	40.0	33.0	40.0
48	37.08214475706319	40.0	36.0	40.0	32.0	40.0
49	36.99282348972277	40.0	36.0	40.0	32.0	40.0
50	36.185285970806426	39.0	35.0	40.0	29.0	40.0
51	36.00898014374167	39.0	35.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	9.0
8	62.0
9	167.0
10	266.0
11	346.0
12	341.0
13	468.0
14	516.0
15	743.0
16	1250.0
17	2362.0
18	4312.0
19	7838.0
20	13525.0
21	21964.0
22	32126.0
23	45534.0
24	61158.0
25	80109.0
26	101976.0
27	127440.0
28	158801.0
29	195720.0
30	241361.0
31	300707.0
32	386748.0
33	517086.0
34	740973.0
35	1143658.0
36	1815182.0
37	2465007.0
38	4039808.0
39	1.3029221E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.921322319939648	33.475960266728954	28.779006441100552	22.82371097223084
2	26.139194890821223	35.75030686125916	18.61775474085716	19.49274350706246
3	32.28583786095235	24.403839402649943	20.87974269274695	22.430580043650757
4	22.269040523307847	23.604752908402528	21.880060469632337	32.24614609865729
5	22.734416254826126	31.879541610269264	23.505049676378604	21.880992458526006
6	31.111852960347203	23.652241266862685	22.365830311059128	22.87007546173099
7	23.40048287206083	23.16578222356495	31.639237280652203	21.794497623722016
8	30.278286792953775	24.35918225414828	23.439340543455256	21.923190409442693
9	30.07966742876991	23.820414355213472	24.133033974323705	21.966884241692917
10	21.92940888996011	24.23236127805438	31.71012325944711	22.1281065725384
11	21.86103693162628	32.19523914228044	23.82962459839796	22.11409932769532
12	21.83998494720459	24.043308505749646	31.88266651420686	22.2340400328389
13	21.81454713269505	24.17438217066087	23.87751629658941	30.133554400054667
14	22.107798612863757	32.299962583387064	23.60519932325075	21.987039480498428
15	21.951138328493585	32.0732464952029	23.645576371497036	22.330038804806478
16	22.16877339884406	23.82337870644249	23.826127682086838	30.18172021262661
17	30.33458988670657	23.736989601470977	23.727771526447043	22.20064898537541
18	22.304581410698333	23.943182354395827	23.54748258247857	30.204753652427275
19	22.279092689232417	31.93052688504054	23.72220308860336	22.068177337123682
20	30.447043353343027	23.87045589333191	23.713940497991427	21.96856025533363
21	22.4407379394078	31.723288581550108	23.75515946897779	22.080814010064305
22	30.473569793535088	24.57644139620551	24.38941707031641	20.560571739942986
23	22.337980290001266	23.840409041310405	31.746862418272308	22.074748250416018
24	22.1935494229207	31.69635880162675	23.731080478611542	22.379011296841007
25	20.48217894304236	29.460932533206506	22.07288427262868	27.984004251122453
26	22.545653260580767	29.00464956728108	26.442244002132608	22.00745317000554
27	22.46605436040598	23.735395822144408	31.66521549208328	22.13333432536633
28	27.724613728783794	23.724693613546105	26.418697576848455	22.131995080821646
29	27.882507527866174	23.770521622044434	26.25703666299419	22.089934187095203
30	27.85109793578166	23.67047378908504	26.294249648105666	22.18417862702764
31	25.528804820183904	24.666546708992538	26.852366106383403	22.952282364440162
32	24.351612781327013	25.46502623568315	27.11510474008377	23.068256242906067
33	24.237295336903216	24.92099534064292	27.485492006922563	23.356217315531303
34	23.669169787817847	24.891022891096117	27.766952652810446	23.672854668275587
35	23.719121259782703	25.03247374326878	27.700068743970707	23.548336252977812
36	23.63487807881846	25.05782932346417	27.694136125592944	23.613156472124427
37	23.90174017598535	24.891132536848314	27.73476770862111	23.472359578545223
38	24.08074469828524	24.95993524635149	27.60716746450268	23.352152590860594
39	24.161518374376413	25.08432443629846	27.407980291959227	23.346176897365897
40	23.743842460983245	25.101272536852232	27.451266868558434	23.703618133606092
41	23.94056260410228	25.32722110477102	27.340332778773835	23.39188351235287
42	24.065116262677545	25.26975889878072	27.34665307320401	23.31847176533773
43	23.902613426083196	25.03701621014548	27.72061949066807	23.339750873103252
44	24.047142191156794	24.93481070541965	27.44185691346816	23.576190189955394
45	23.970370585020785	24.75879402986711	27.503892913692933	23.76694247141917
46	23.83657927182298	24.850348232951017	27.437670795286095	23.87540169993991
47	24.035414011591513	24.91119770950024	27.355557874650234	23.697830404258013
48	24.132423090847183	24.81582940060779	27.42632245993378	23.62542504861125
49	23.709860109641838	25.25192188445022	27.333691378926517	23.70452662698143
50	23.930533933696037	25.04532187587435	27.54127036743271	23.482873822996904
51	24.05116384071057	24.917169487075213	27.3227659629041	23.708900709310118
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4023.0
1	2571.0
2	1119.0
3	967.5
4	816.0
5	909.5
6	1003.0
7	1253.0
8	1503.0
9	1999.0
10	2495.0
11	3851.5
12	5208.0
13	7639.5
14	10071.0
15	13426.5
16	16782.0
17	24461.5
18	32141.0
19	53371.5
20	74602.0
21	101641.0
22	128680.0
23	402121.5
24	675563.0
25	475586.0
26	290782.5
27	305956.0
28	342321.5
29	378687.0
30	424914.5
31	471142.0
32	529562.5
33	587983.0
34	659700.5
35	731418.0
36	820451.0
37	909484.0
38	996102.5
39	1082721.0
40	1176179.5
41	1269638.0
42	1363456.5
43	1457275.0
44	1556748.0
45	1656221.0
46	1748287.0
47	1840353.0
48	1877970.0
49	1915587.0
50	1948326.5
51	1981066.0
52	1972477.0
53	1963888.0
54	1908041.5
55	1852195.0
56	1729866.0
57	1607537.0
58	1467036.5
59	1326536.0
60	1170738.5
61	1014941.0
62	875311.5
63	735682.0
64	619215.0
65	502748.0
66	406760.5
67	310773.0
68	253368.5
69	195964.0
70	149354.5
71	102745.0
72	77031.0
73	51317.0
74	39428.5
75	20873.0
76	14206.0
77	10511.0
78	6816.0
79	5105.0
80	3394.0
81	2590.0
82	1786.0
83	1320.0
84	854.0
85	618.5
86	383.0
87	274.0
88	165.0
89	130.0
90	95.0
91	66.0
92	37.0
93	30.0
94	23.0
95	16.5
96	10.0
97	6.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2.5536785E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.23615593456318
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.07019943969628	36.70765933222213
2	14.69666892255887	14.76608303424632
3	4.820767015784884	7.265304105875046
4	2.229493775339125	4.480047878122969
5	1.180969677070601	2.9663688425654717
6	0.7522318803777921	2.267354282496506
7	0.5073209354872299	1.7840097536803463
8	0.3592337331923943	1.4437217470086705
9	0.3129716991751055	1.4150245574579217
>10	1.9408727383232522	17.83081047101568
>50	0.09900657072133116	3.3393929289046795
>100	0.028452092081142487	2.51057978569747
>500	0.0013248204505651828	0.4296608387095651
>1k	4.13866879663964E-4	0.3886427856894021
>5k	4.046270108413919E-5	0.13777983161773855
>10k+	3.237016086602338E-5	2.2675598246902138
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	483442	1.8931200619028592	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATC	32562	0.12751017796484562	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9579598606480809E-4	0.0	0.0	3.915919721296162E-6	7.831839442592323E-6
2	2.8977805937591594E-4	3.915919721296162E-6	0.0	3.915919721296162E-6	1.1747759163888484E-5
3	2.8977805937591594E-4	7.831839442592323E-6	0.0	3.915919721296162E-6	2.3495518327776968E-5
4	2.8977805937591594E-4	7.831839442592323E-6	0.0	7.831839442592323E-6	3.9159197212961614E-5
5	2.8977805937591594E-4	7.831839442592323E-6	0.0	7.831839442592323E-6	5.09069563768501E-5
6	2.976098988185083E-4	7.831839442592323E-6	0.0	7.831839442592323E-6	6.265471554073859E-5
7	2.976098988185083E-4	1.5663678885184646E-5	0.0	1.1747759163888484E-5	6.657063526203474E-5
8	2.976098988185083E-4	1.9579598606480807E-5	0.0	1.1747759163888484E-5	6.657063526203474E-5
9	2.976098988185083E-4	1.9579598606480807E-5	0.0	1.1747759163888484E-5	7.048655498333091E-5
10	3.0152581853980443E-4	2.3495518327776968E-5	0.0	1.1747759163888484E-5	8.22343141472194E-5
11	3.0152581853980443E-4	3.524327749166546E-5	0.0	1.1747759163888484E-5	8.615023386851556E-5
12	3.0152581853980443E-4	3.524327749166546E-5	0.0	1.5663678885184646E-5	9.789799303240404E-5
13	3.2110541714628526E-4	3.9159197212961614E-5	0.0	3.524327749166546E-5	1.018139127537002E-4
14	3.406850157527661E-4	5.09069563768501E-5	0.0	3.9159197212961614E-5	1.1747759163888484E-4
15	3.759282932444315E-4	5.4822876098146264E-5	0.0	4.307511693425778E-5	1.1747759163888484E-4
16	3.8376013268702383E-4	5.4822876098146264E-5	0.0	4.307511693425778E-5	1.3314127052406949E-4
17	3.9159197212961617E-4	5.4822876098146264E-5	0.0	4.307511693425778E-5	1.4488902968795797E-4
18	4.2683524962128163E-4	5.873879581944242E-5	0.0	4.6991036655553936E-5	1.4880494940925414E-4
19	4.464148482277624E-4	6.265471554073859E-5	0.0	4.6991036655553936E-5	1.5663678885184646E-4
20	4.8165812571942786E-4	6.265471554073859E-5	0.0	4.6991036655553936E-5	1.7621638745832728E-4
21	5.129854834897971E-4	6.657063526203474E-5	0.0	5.09069563768501E-5	1.8013230717962343E-4
22	5.208173229323895E-4	7.048655498333091E-5	0.0	5.09069563768501E-5	1.9188006634351191E-4
23	5.443128412601665E-4	7.440247470462707E-5	0.0	5.4822876098146264E-5	2.036278255074004E-4
24	5.678083595879435E-4	7.440247470462707E-5	0.0	5.4822876098146264E-5	2.0754374522869657E-4
25	5.913038779157204E-4	7.440247470462707E-5	0.0	5.4822876098146264E-5	2.1145966494999271E-4
26	6.343789948499782E-4	7.440247470462707E-5	0.0	5.873879581944242E-5	2.1929150439258506E-4
27	6.813700315055322E-4	9.398207331110787E-5	0.0	5.873879581944242E-5	2.1929150439258506E-4
28	7.361929076036784E-4	9.398207331110787E-5	0.0	5.873879581944242E-5	2.1929150439258506E-4
29	7.870998639805285E-4	9.789799303240404E-5	0.0	7.048655498333091E-5	2.1929150439258506E-4
30	8.771660175703402E-4	1.018139127537002E-4	0.0	7.831839442592323E-5	2.3887110299906586E-4
31	0.0010612142444712599	1.018139127537002E-4	0.0	8.22343141472194E-5	2.4278702272036203E-4
32	0.0012335147122082908	1.0572983247499636E-4	0.0	8.22343141472194E-5	2.545347818842505E-4
33	0.001358824143289768	1.1356167191758869E-4	0.0	9.398207331110787E-5	2.6236662132684283E-4
34	0.0014488902968795797	1.2139351136018101E-4	0.0	9.398207331110787E-5	2.6236662132684283E-4
35	0.0015624519687971684	1.2139351136018101E-4	0.0	1.018139127537002E-4	2.858621396546198E-4
36	0.0017034250787638303	1.2139351136018101E-4	0.0	1.0572983247499636E-4	2.9369397909721214E-4
37	0.0019148847437138231	1.2139351136018101E-4	0.0	1.0572983247499636E-4	3.0152581853980443E-4
38	0.002102848890336039	1.3705719024536566E-4	0.0	1.0572983247499636E-4	3.0935765798239677E-4
39	0.0023182244750073275	1.4097310996666183E-4	0.0	1.0572983247499636E-4	3.2110541714628526E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	215650	0.0	42.60967	1
TCAACGC	214110	0.0	42.15742	12
CAACGCA	214485	0.0	42.10889	13
ATCAACG	221115	0.0	42.037857	11
GTATCAA	219920	0.0	41.980835	9
GGTATCA	220030	0.0	41.95678	8
GTGGTAT	219535	0.0	41.930447	6
AACGCAG	215755	0.0	41.894394	14
ACGCAGA	214740	0.0	41.890194	15
AGTGGTA	219320	0.0	41.864857	5
TGGTATC	220260	0.0	41.73216	7
TATCAAC	222725	0.0	41.686497	10
CGCAGAG	207060	0.0	41.47385	16
AGAGTAC	202925	0.0	41.35432	19
AGCAGTG	223515	0.0	41.14556	2
GCAGTGG	224705	0.0	40.921654	3
CAGTGGT	224775	0.0	40.859863	4
CAGAGTA	207665	0.0	40.82212	18
GCAGAGT	210915	0.0	40.459785	17
GTACATG	139630	0.0	40.049774	22
>>END_MODULE