FastQCFastQC Report
Thu 26 May 2016
SRR805432_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805432_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17002584
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3461382.0357964412938645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1208450.042.492041
TCAACGC1203850.042.06192812
CAACGCA1203850.042.04323613
AACGCAG1210400.041.77668414
ACGCAGA1209800.041.75462715
GTATCAA1225650.041.7323469
AGTGGTA1221200.041.654115
ATCAACG1217400.041.61224711
CGCAGAG1211100.041.5723316
TGGTATC1225700.041.5654347
GGTATCA1227550.041.565118
GTGGTAT1233400.041.2986456
AGAGTAC1216300.041.13931719
TATCAAC1237100.041.0350810
CAGTGGT1241650.040.9644434
GCAGTGG1256150.040.702943
CAGAGTA1251250.040.4055918
GCAGAGT1250550.040.37063617
AGCAGTG1271650.040.2882082
GTACATG693450.040.00324222
Produced by FastQC (version 0.11.5)