##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805432_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17002584
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.67083809143363	35.0	35.0	35.0	33.0	37.0
2	35.627045159723956	36.0	35.0	37.0	35.0	37.0
3	37.89174627809514	39.0	38.0	39.0	35.0	39.0
4	37.90978535968415	39.0	38.0	39.0	35.0	39.0
5	37.93051315023646	39.0	38.0	39.0	35.0	39.0
6	37.93523278579303	39.0	38.0	39.0	35.0	39.0
7	37.84835740261598	39.0	38.0	39.0	35.0	39.0
8	38.74708732507953	40.0	39.0	40.0	36.0	40.0
9	38.74923723358755	40.0	39.0	40.0	36.0	40.0
10	38.521694525961465	40.0	38.0	40.0	36.0	40.0
11	38.725377683768535	40.0	39.0	40.0	36.0	40.0
12	38.78821672047025	40.0	39.0	40.0	36.0	40.0
13	38.86057401627894	40.0	39.0	40.0	36.0	40.0
14	38.82408568015309	40.0	39.0	40.0	36.0	40.0
15	38.79509196955004	40.0	39.0	40.0	36.0	40.0
16	38.53914910815909	40.0	39.0	40.0	36.0	40.0
17	38.31625369414437	40.0	39.0	40.0	35.0	40.0
18	38.52852625224495	40.0	39.0	40.0	35.0	40.0
19	38.53971455162345	40.0	39.0	40.0	36.0	40.0
20	38.44083216998075	40.0	39.0	40.0	35.0	40.0
21	38.38117453205936	40.0	38.0	40.0	35.0	40.0
22	38.22185169030778	40.0	38.0	40.0	35.0	40.0
23	38.00599214801703	40.0	38.0	40.0	34.0	40.0
24	38.128252505619145	40.0	38.0	40.0	34.0	40.0
25	37.964574325878935	40.0	38.0	40.0	34.0	40.0
26	37.9735647240443	40.0	38.0	40.0	34.0	40.0
27	37.731096932089855	40.0	38.0	40.0	33.0	40.0
28	37.82719473698821	40.0	38.0	40.0	34.0	40.0
29	37.854987806559286	40.0	38.0	40.0	34.0	40.0
30	37.767229557577835	40.0	38.0	40.0	33.0	40.0
31	37.66002308825529	40.0	38.0	40.0	33.0	40.0
32	37.56219454642894	40.0	38.0	40.0	33.0	40.0
33	37.46874622116262	40.0	38.0	40.0	33.0	40.0
34	37.18481837819475	40.0	37.0	40.0	32.0	40.0
35	37.20234106768712	40.0	37.0	40.0	32.0	40.0
36	37.09246423955324	39.0	37.0	40.0	31.0	40.0
37	36.5488129333753	39.0	36.0	40.0	30.0	40.0
38	36.638147178099516	39.0	35.0	40.0	30.0	40.0
39	36.625467281914325	39.0	35.0	40.0	31.0	40.0
40	36.48469015062652	39.0	35.0	40.0	30.0	40.0
41	36.62946085136236	39.0	35.0	40.0	31.0	40.0
42	36.58014334762293	39.0	35.0	40.0	30.0	40.0
43	36.51730854557166	39.0	35.0	40.0	30.0	40.0
44	36.39243176213686	39.0	35.0	40.0	30.0	40.0
45	36.25069142431527	39.0	35.0	40.0	30.0	40.0
46	36.01539154283843	39.0	35.0	40.0	29.0	40.0
47	35.82787151647067	38.0	35.0	40.0	28.0	40.0
48	30.659880404060935	37.0	28.0	40.0	3.0	40.0
49	31.883623159867934	37.0	28.0	40.0	12.0	40.0
50	33.45426130522278	37.0	30.0	40.0	22.0	40.0
51	33.89553876046135	36.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	69.0
9	185.0
10	224.0
11	285.0
12	286.0
13	325.0
14	408.0
15	591.0
16	970.0
17	1842.0
18	3439.0
19	6723.0
20	11503.0
21	18754.0
22	28240.0
23	39939.0
24	54471.0
25	71004.0
26	90830.0
27	113555.0
28	140091.0
29	173076.0
30	213043.0
31	264327.0
32	332191.0
33	432412.0
34	596692.0
35	896906.0
36	1509569.0
37	2803038.0
38	3771836.0
39	5425755.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.76052510606623	43.136825555456745	21.255222147410063	22.847427191066956
2	25.839966442747762	39.55711084856278	19.214508806426128	15.388413902263325
3	31.129062500147036	25.225789209451925	23.079974196863255	20.565174093537784
4	20.85041897161043	25.84911211142965	23.006326567773463	30.294142349186455
5	19.919195811648393	33.165311813780775	25.998277673558324	20.917214701012504
6	27.936053719834586	24.457658906434457	24.839665547307398	22.76662182642356
7	22.804251400845896	25.00495218844383	30.9564299167703	21.23436649393998
8	27.19400180584316	25.457795121023956	25.955395956285233	21.39280711684765
9	27.829269950967454	24.600895957932043	26.200141107963354	21.36969298313715
10	21.644562967605392	25.68004369218232	31.496606633438777	21.17878670677351
11	21.134881615641483	32.25470316747149	25.216408282411663	21.39400693447537
12	21.162248044179638	25.241298616727903	31.821992468909432	21.774460870183027
13	21.238642314603474	25.949626245046048	24.848634772220503	27.96309666812997
14	21.56642778532957	32.4989425136791	24.755066641635175	21.179563059356155
15	21.467001721620665	31.992643000616848	24.775016550425512	21.765338727336975
16	21.941347268156417	25.232717568106118	24.86746720380855	27.95846795992891
17	28.826288992308463	24.892151687061215	25.068618981679492	21.212940338950833
18	21.634229244213703	25.06379618533277	24.86274439226414	28.439230178189383
19	21.821371386843317	32.06641414034478	24.654011413794517	21.458203059017382
20	28.609380785885254	25.43558085053425	24.748338252585608	21.206700110994895
21	21.957027237742217	31.6932826210416	24.774357827022055	21.575332314194124
22	28.380968445737427	25.171950334137446	25.057208951298225	21.389872268826903
23	21.687315292781378	25.294772841586905	31.597356025413546	21.42055584021817
24	21.78747653886021	31.414313259678643	24.830866884704115	21.96734331675703
25	21.837145459772465	25.194199893380915	24.86396185426874	28.10469279257788
26	21.786064988709953	28.886379858496802	27.966878446240877	21.36067670655237
27	22.049295565897513	24.806017720600586	31.499282697265308	21.64540401623659
28	25.532107354976162	25.1687978721352	27.69105566542121	21.60803910746743
29	25.69389452803174	25.14240188432535	27.7355606653671	21.428142922275814
30	25.70926278029269	24.783127082330545	27.72590330975574	21.781706827621026
31	23.909401065155745	25.92236568276916	27.974242032858065	22.193991219217033
32	23.131089956679528	26.303990028809736	28.367682229948105	22.197237784562628
33	23.16620226666723	25.724195804590643	28.460768080898763	22.648833847843363
34	22.929591172729978	26.02445604738668	28.542749737334045	22.5032030425493
35	22.719840701860374	26.01953914769661	28.674088597356732	22.586531553086285
36	22.76731583858077	25.78613344889224	28.6342123056119	22.81233840691509
37	23.077692190787	25.95221408698819	28.48398808087053	22.48610564135428
38	22.963586005515396	26.052528250999966	28.624713749392445	22.359171994092193
39	23.00382106625675	25.66727504478143	28.60027040595712	22.7286334830047
40	22.719852464778295	26.13395705029306	28.36703526946257	22.779155215466073
41	22.832552981358596	26.171398418028698	28.514201135545044	22.481847465067663
42	22.979977631635286	25.868503281618842	28.509554782967108	22.641964303778767
43	22.871741142405178	26.207787004610594	28.516389038277946	22.404082814706282
44	23.008108649838167	25.97648098665473	28.504108551970685	22.51130181153641
45	22.975254820090875	25.62204074392457	28.649468810152623	22.753235625831934
46	22.93390816360619	25.905862308929045	28.395042777027303	22.765186750437465
47	23.038680473509203	25.96857630581328	28.398012913801807	22.594730306875707
48	20.936617634119614	34.781183848290354	25.109642157921407	19.172556359668626
49	22.969426294262096	26.12494077370828	28.238484220986642	22.66714871104298
50	23.08884931843301	25.964935682717403	28.40611756424788	22.540097434601705
51	23.128613862457613	25.82751539413068	28.244383324322936	22.799487419088766
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3400.0
1	2229.5
2	1059.0
3	1021.5
4	984.0
5	1139.0
6	1294.0
7	1506.0
8	1718.0
9	2167.0
10	2616.0
11	3847.0
12	5078.0
13	6886.5
14	8695.0
15	12140.5
16	15586.0
17	23212.5
18	30839.0
19	41901.0
20	52963.0
21	75486.0
22	98009.0
23	332310.0
24	566611.0
25	407894.0
26	269387.0
27	289597.0
28	331905.0
29	374213.0
30	413438.5
31	452664.0
32	498483.5
33	544303.0
34	602449.0
35	660595.0
36	737119.5
37	813644.0
38	866882.5
39	920121.0
40	970242.0
41	1020363.0
42	1053584.5
43	1086806.0
44	1106679.0
45	1126552.0
46	1140823.0
47	1155094.0
48	1160500.0
49	1165906.0
50	1153700.0
51	1141494.0
52	1114900.5
53	1088307.0
54	1041798.5
55	995290.0
56	927048.0
57	858806.0
58	785030.5
59	711255.0
60	627313.0
61	543371.0
62	464883.0
63	386395.0
64	323538.0
65	260681.0
66	210909.0
67	161137.0
68	129343.5
69	97550.0
70	75103.0
71	52656.0
72	40180.5
73	27705.0
74	20932.5
75	10773.5
76	7387.0
77	5575.5
78	3764.0
79	2931.0
80	2098.0
81	1666.0
82	1234.0
83	945.5
84	657.0
85	486.0
86	315.0
87	241.0
88	167.0
89	149.5
90	132.0
91	105.0
92	78.0
93	55.5
94	33.0
95	23.5
96	14.0
97	11.0
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1.7002584E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.92076815280884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.20611166947903	31.08791697062066
2	16.388990497271422	14.724120847730335
3	6.050079225391074	8.153226185697532
4	2.727546308024496	4.900939013152724
5	1.4240513279907094	3.198473977118509
6	0.8739656237765538	2.355552429551492
7	0.597602862460118	1.8791345742418106
8	0.40173414418324777	1.4436965079938202
9	0.32603537318126596	1.3181183467461142
>10	1.7669603920845367	13.88780444758057
>50	0.11693687511339337	3.716809712984764
>100	0.11729189384452729	9.863672654258897
>500	0.00214257099717463	0.6082875088974031
>1k	4.410086870883824E-4	0.3939836446647252
>5k	8.267063647939646E-5	0.25871611843874887
>10k+	2.7556878826465483E-5	2.209547060321746
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	346138	2.0357964412938645	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.1170212715902476E-5	0.0	0.0	0.0	1.764437687824392E-5
2	4.705167167531712E-5	5.88145895941464E-6	0.0	0.0	1.764437687824392E-5
3	4.705167167531712E-5	5.88145895941464E-6	0.0	0.0	1.764437687824392E-5
4	5.8814589594146396E-5	1.176291791882928E-5	0.0	0.0	2.9407294797073198E-5
5	5.8814589594146396E-5	1.176291791882928E-5	0.0	0.0	3.528875375648784E-5
6	5.8814589594146396E-5	1.176291791882928E-5	0.0	5.88145895941464E-6	4.705167167531712E-5
7	5.8814589594146396E-5	1.176291791882928E-5	0.0	5.88145895941464E-6	4.705167167531712E-5
8	5.8814589594146396E-5	1.176291791882928E-5	0.0	1.176291791882928E-5	5.8814589594146396E-5
9	5.8814589594146396E-5	1.764437687824392E-5	0.0	1.176291791882928E-5	7.057750751297568E-5
10	6.469604855356104E-5	4.705167167531712E-5	0.0	1.764437687824392E-5	7.645896647239031E-5
11	6.469604855356104E-5	4.705167167531712E-5	0.0	1.764437687824392E-5	1.1174772022887815E-4
12	7.645896647239031E-5	4.705167167531712E-5	0.0	1.764437687824392E-5	1.1762917918829279E-4
13	8.82218843912196E-5	4.705167167531712E-5	0.0	1.764437687824392E-5	1.4115501502595136E-4
14	9.410334335063424E-5	5.2933130634731754E-5	0.0	1.764437687824392E-5	1.5291793294478062E-4
15	9.998480231004888E-5	5.2933130634731754E-5	0.0	2.352583583765856E-5	1.646808508636099E-4
16	9.998480231004888E-5	5.8814589594146396E-5	0.0	3.528875375648784E-5	1.646808508636099E-4
17	1.0586626126946351E-4	5.8814589594146396E-5	0.0	4.1170212715902476E-5	1.764437687824392E-4
18	1.2351063814770744E-4	5.8814589594146396E-5	0.0	4.705167167531712E-5	1.9996960462009776E-4
19	1.2939209710712208E-4	5.8814589594146396E-5	0.0	4.705167167531712E-5	1.9996960462009776E-4
20	1.4703647398536598E-4	5.8814589594146396E-5	0.0	4.705167167531712E-5	2.0585106357951237E-4
21	1.646808508636099E-4	5.8814589594146396E-5	0.0	4.705167167531712E-5	2.1761398149834166E-4
22	1.7056230982302455E-4	5.8814589594146396E-5	0.0	4.705167167531712E-5	2.5878419421424415E-4
23	1.7056230982302455E-4	5.8814589594146396E-5	0.0	5.2933130634731754E-5	2.7054711213307344E-4
24	1.8232522774185383E-4	5.8814589594146396E-5	0.0	5.8814589594146396E-5	2.7054711213307344E-4
25	1.9996960462009776E-4	5.8814589594146396E-5	0.0	6.469604855356104E-5	2.764285710924881E-4
26	2.4113981733600023E-4	6.469604855356104E-5	0.0	6.469604855356104E-5	2.9407294797073195E-4
27	2.881914890113173E-4	6.469604855356104E-5	0.0	7.057750751297568E-5	3.0583586588956124E-4
28	3.3524316068663445E-4	6.469604855356104E-5	0.0	7.645896647239031E-5	3.117173248489759E-4
29	3.8817629132136623E-4	6.469604855356104E-5	0.0	7.645896647239031E-5	3.117173248489759E-4
30	4.4110942195609796E-4	6.469604855356104E-5	0.0	7.645896647239031E-5	3.175987838083905E-4
31	4.940425525908297E-4	6.469604855356104E-5	0.0	8.234042543180495E-5	3.175987838083905E-4
32	6.234346496979518E-4	7.057750751297568E-5	0.0	8.234042543180495E-5	3.2348024276780517E-4
33	7.116565340891714E-4	8.234042543180495E-5	0.0	8.82218843912196E-5	3.4700607860546374E-4
34	8.586930080745373E-4	8.234042543180495E-5	0.0	8.82218843912196E-5	3.528875375648784E-4
35	9.292705155875131E-4	8.82218843912196E-5	0.0	9.410334335063424E-5	3.7641337340253695E-4
36	9.822036462222448E-4	8.82218843912196E-5	0.0	9.998480231004888E-5	3.940577502807809E-4
37	0.001046899694775806	8.82218843912196E-5	0.0	1.5291793294478062E-4	3.940577502807809E-4
38	0.0011762917918829278	9.410334335063424E-5	0.0	1.5291793294478062E-4	4.1170212715902475E-4
39	0.0012586322173147328	9.998480231004888E-5	0.0	3.940577502807809E-4	4.293465040372687E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	120845	0.0	42.49204	1
TCAACGC	120385	0.0	42.061928	12
CAACGCA	120385	0.0	42.043236	13
AACGCAG	121040	0.0	41.776684	14
ACGCAGA	120980	0.0	41.754627	15
GTATCAA	122565	0.0	41.732346	9
AGTGGTA	122120	0.0	41.65411	5
ATCAACG	121740	0.0	41.612247	11
CGCAGAG	121110	0.0	41.57233	16
TGGTATC	122570	0.0	41.565434	7
GGTATCA	122755	0.0	41.56511	8
GTGGTAT	123340	0.0	41.298645	6
AGAGTAC	121630	0.0	41.139317	19
TATCAAC	123710	0.0	41.03508	10
CAGTGGT	124165	0.0	40.964443	4
GCAGTGG	125615	0.0	40.70294	3
CAGAGTA	125125	0.0	40.40559	18
GCAGAGT	125055	0.0	40.370636	17
AGCAGTG	127165	0.0	40.288208	2
GTACATG	69345	0.0	40.003242	22
>>END_MODULE