FastQCFastQC Report
Thu 26 May 2016
SRR805431_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805431_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5016171
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT317300.6325541932282612No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA168670.33625249218975983No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT61420.12244399164223069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT108450.035.726141
TCAACGC122350.031.90641812
CAACGCA123050.031.81633613
ATCAACG123800.031.58723611
ACGCAGA124950.031.20648215
AACGCAG125400.031.20215414
GGTATCA126800.030.822168
CGCAGAG126950.030.76801716
GTGGTAT127850.030.516236
GTATCAA128550.030.4375749
TGGTATC128700.030.4195797
AGTGGTA128800.030.4134335
TATCAAC129500.030.1621610
AGCAGTG130000.030.080772
AGAGTAC129450.029.75666219
GCAGTGG134350.029.240793
CAGAGTA133900.028.91896818
GCAGAGT136000.028.62132517
CAGTGGT141250.027.8761084
GAGTACT67350.027.6614720
Produced by FastQC (version 0.11.5)