##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805431_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5016171
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	34.98621298994791	37.0	35.0	37.0	33.0	37.0
2	35.60715912595484	37.0	35.0	37.0	33.0	37.0
3	37.68126784354042	39.0	38.0	39.0	35.0	39.0
4	37.82556695136589	39.0	38.0	39.0	35.0	39.0
5	37.90396220543518	39.0	38.0	39.0	35.0	39.0
6	37.509737407277385	39.0	37.0	39.0	35.0	39.0
7	37.64582786352379	39.0	37.0	39.0	35.0	39.0
8	38.64971688564844	40.0	39.0	40.0	36.0	40.0
9	38.60320331982303	40.0	38.0	40.0	36.0	40.0
10	38.6059623964175	40.0	38.0	40.0	36.0	40.0
11	38.48383059508936	40.0	38.0	40.0	36.0	40.0
12	38.33473699361525	40.0	38.0	40.0	35.0	40.0
13	38.38944984132319	40.0	38.0	40.0	35.0	40.0
14	38.276487783211536	40.0	38.0	40.0	35.0	40.0
15	38.212830463714255	40.0	38.0	40.0	35.0	40.0
16	38.10688511217022	40.0	38.0	40.0	34.0	40.0
17	37.955470218220235	40.0	38.0	40.0	34.0	40.0
18	37.76528172584228	39.0	37.0	40.0	34.0	40.0
19	37.59528632496779	39.0	37.0	40.0	34.0	40.0
20	37.35984179965157	39.0	37.0	40.0	33.0	40.0
21	37.15876691604014	39.0	36.0	40.0	33.0	40.0
22	37.01697091267423	38.0	36.0	40.0	33.0	40.0
23	36.826486178401815	38.0	36.0	40.0	32.0	40.0
24	36.53028674660413	38.0	36.0	40.0	31.0	40.0
25	36.34652247700487	38.0	35.0	40.0	31.0	40.0
26	36.10827501694021	38.0	35.0	39.0	31.0	40.0
27	35.84719201159609	38.0	34.0	39.0	30.0	40.0
28	35.649540655611624	38.0	34.0	39.0	30.0	40.0
29	35.25534895042453	37.0	34.0	39.0	29.0	40.0
30	35.11004788313636	37.0	34.0	39.0	29.0	40.0
31	34.79987205380359	37.0	33.0	38.0	29.0	40.0
32	34.58324925525864	36.0	33.0	38.0	28.0	40.0
33	34.27367787102952	36.0	33.0	38.0	27.0	39.0
34	33.95167489306086	36.0	32.0	38.0	27.0	39.0
35	33.686144072839625	35.0	31.0	38.0	26.0	39.0
36	33.454118091269216	35.0	31.0	38.0	26.0	39.0
37	33.3768968402393	35.0	31.0	38.0	26.0	39.0
38	33.17972612975116	35.0	31.0	38.0	25.0	39.0
39	32.880375688946806	35.0	31.0	38.0	25.0	39.0
40	32.616213043773826	34.0	30.0	37.0	24.0	38.0
41	32.305386718275756	34.0	30.0	37.0	24.0	38.0
42	32.01178348983717	34.0	30.0	37.0	24.0	38.0
43	31.66140667852033	33.0	29.0	37.0	23.0	38.0
44	31.23058563992336	33.0	29.0	37.0	23.0	38.0
45	30.88892802896871	33.0	28.0	36.0	22.0	38.0
46	30.55259519661511	33.0	28.0	36.0	20.0	38.0
47	30.156925471639624	32.0	27.0	35.0	19.0	38.0
48	29.755953096495315	31.0	27.0	35.0	19.0	38.0
49	29.230419776359298	31.0	26.0	35.0	17.0	37.0
50	29.035450745199874	31.0	26.0	35.0	17.0	37.0
51	30.78218984161425	33.0	28.0	35.0	22.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	7.0
8	35.0
9	69.0
10	99.0
11	162.0
12	273.0
13	419.0
14	660.0
15	1069.0
16	1670.0
17	2295.0
18	3596.0
19	5240.0
20	7645.0
21	11223.0
22	15663.0
23	21014.0
24	28056.0
25	35589.0
26	44732.0
27	55697.0
28	68303.0
29	83869.0
30	102716.0
31	128885.0
32	169150.0
33	240477.0
34	401004.0
35	833018.0
36	1527277.0
37	1084405.0
38	141064.0
39	790.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.493599002107384	31.039073428716847	20.39804863111724	35.06927893805853
2	21.370423775425518	25.8014928119476	32.73427082130972	20.09381259131716
3	16.810172539971223	25.88994673427202	32.0247854389334	25.27509528682336
4	16.783598485777297	27.997689871417858	32.03666701155124	23.1820446312536
5	22.310044852936635	29.19318340622758	29.35848877560195	19.13828296523384
6	22.64641695827355	26.730109479920046	30.23922031366156	20.38425324814485
7	22.378583186259	28.01543248824651	29.88331139428859	19.722672931205896
8	21.214288747333374	26.059897080861077	30.565784140931402	22.160030030874147
9	21.25186721106597	25.489302497861416	31.89440710852959	21.364423182543018
10	21.016269182210895	26.850041595471925	30.38564674130926	21.74804248100792
11	20.835972298392537	28.79427356045079	28.67783016169106	21.691923979465614
12	21.242716805308273	27.05741092159737	29.755405068926077	21.94446720416828
13	21.37670346565139	27.537956740310488	27.587177550366604	23.498162243671516
14	21.68570409581332	29.08844216036495	27.376518862694276	21.849334881127458
15	21.46986217176408	28.883066386692157	27.34559886415355	22.301472577390204
16	22.02271413793509	27.23114901784648	27.13446172389259	23.61167512032584
17	23.772116221715727	26.99497285878013	27.107827863125085	22.125083056379058
18	21.954813741397572	27.221560030549195	27.02088904066468	23.802737187388548
19	22.426089541205833	28.583993647744464	26.945134047463693	22.04478276358601
20	23.672159501739472	27.291852690029906	26.985124709664003	22.050863098566616
21	22.35866759725695	28.632078132902567	27.08629749663638	21.922956773204103
22	23.548758604919968	26.93078046980456	27.29984683536506	22.220614089910413
23	21.757412177535414	27.302916906142155	28.954555177644465	21.98511573867797
24	21.79658548323014	28.543803630298886	27.22648410510726	22.433126781363715
25	21.811118480610013	27.06552468007969	27.32000165066143	23.803355188648872
26	21.923355483694635	27.66592287224658	28.478554658523404	21.932166985535382
27	22.135110625215926	26.769960593448666	29.027479326362677	22.067449454972728
28	22.957909529001306	26.841349706778338	28.000421038278	22.200319725942357
29	23.229929761166435	26.723570627875326	28.06832143481552	21.97817817614272
30	22.85609880524408	26.87464203273772	27.993064829727693	22.276194332290505
31	22.746373678249803	26.903189703859777	27.90405271271653	22.446383905173885
32	22.69181014762057	27.112931357403884	27.973986532755763	22.221271962219788
33	22.57231661360827	27.062394802729013	27.907162654542677	22.45812592912004
34	22.513965333318982	26.980958185037952	27.93293928775554	22.572137193887528
35	22.377965184998676	27.15950074269797	28.068760016355103	22.393774055948253
36	22.567272925903044	26.859630582769206	28.211538243014445	22.361558248313305
37	22.54737727242552	27.0764094764712	27.89434411227209	22.481869138831193
38	22.617689867430755	27.1520249210005	28.05295114540553	22.177334066163215
39	22.52945523587613	27.106093472491267	27.98977546818081	22.374675823451792
40	22.486314760800617	27.13996392866192	27.866195151640564	22.507526158896894
41	22.45515553596558	27.31551615764295	27.98598771852076	22.24334058787071
42	22.43874859928021	27.06576390637401	28.121609091875055	22.37387840247073
43	22.424135859802227	27.084244137610142	27.97039813834098	22.521221864246655
44	22.51496210954531	27.014350188619968	28.068221757192884	22.40246594464184
45	22.67815431331986	26.850819080928463	28.08207694673886	22.388949659012823
46	22.44263602656289	26.907715067927306	27.955247139700777	22.69440176580902
47	22.434243170737204	26.995710473187618	28.030862584230082	22.5391837718451
48	22.277888851875264	26.92866730420474	28.0719098292303	22.72153401468969
49	22.70119977967258	26.967780803325887	27.796959074959766	22.534060342041766
50	22.550287859006403	27.111476064113443	27.847017177045995	22.491218899834156
51	22.485038887230917	27.05960382929529	27.93317851404986	22.522178769423927
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	18637.0
1	11962.0
2	5287.0
3	4082.0
4	2877.0
5	2638.5
6	2400.0
7	2491.5
8	2583.0
9	3045.5
10	3508.0
11	4585.0
12	5662.0
13	7192.5
14	8723.0
15	10834.0
16	12945.0
17	16811.5
18	20678.0
19	26785.5
20	32893.0
21	41696.0
22	50499.0
23	80836.5
24	111174.0
25	105050.5
26	109763.0
27	120599.0
28	130058.5
29	139518.0
30	149422.0
31	159326.0
32	170827.5
33	182329.0
34	194019.5
35	205710.0
36	220028.5
37	234347.0
38	245972.5
39	257598.0
40	269304.0
41	281010.0
42	292549.5
43	304089.0
44	315896.0
45	327703.0
46	335885.5
47	344068.0
48	345313.0
49	346558.0
50	343739.0
51	340920.0
52	329720.5
53	318521.0
54	301435.0
55	284349.0
56	260971.5
57	237594.0
58	211149.5
59	184705.0
60	160606.5
61	136508.0
62	115552.0
63	94596.0
64	78481.0
65	62366.0
66	49350.0
67	36334.0
68	28785.0
69	21236.0
70	15949.0
71	10662.0
72	7710.5
73	4759.0
74	3433.0
75	1559.5
76	1012.0
77	742.0
78	472.0
79	330.0
80	188.0
81	152.0
82	116.0
83	85.5
84	55.0
85	33.0
86	11.0
87	9.0
88	7.0
89	4.0
90	1.0
91	2.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5016171.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.50622348842407
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.82142091183724	47.70256272995408
2	17.520963229119932	22.954642660383815
3	5.653579349692114	11.110338971714127
4	2.045402330794923	5.35946328819183
5	0.8096554407369182	2.651873512476553
6	0.41029423709717117	1.612609559477985
7	0.21086558739953384	0.9669105805948178
8	0.11489505159129672	0.6021072741802799
9	0.08565579633992998	0.5049888964310151
>10	0.29782932572763743	3.4308674426211825
>50	0.02278753903742558	1.019535692936931
>100	0.006213405071441015	0.6242411418316126
>500	2.6941281380241165E-4	0.122588871509834
>1k	6.735309623134005E-5	0.12971418104640434
>5k	3.3676548115670026E-5	0.13549396258282578
>10k+	6.735309623134005E-5	1.0720612340666857
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	31730	0.6325541932282612	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16867	0.33625249218975983	No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6142	0.12244399164223069	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.974209810630458E-5	0.0	0.0	0.0	0.0
2	7.974209810630458E-5	0.0	0.0	0.0	0.0
3	7.974209810630458E-5	0.0	0.0	0.0	0.0
4	9.967762263288073E-5	0.0	0.0	0.0	0.0
5	4.186460150580991E-4	0.0	0.0	0.0	1.9935524526576146E-5
6	4.186460150580991E-4	0.0	0.0	0.0	5.980657357972844E-5
7	4.3858153958467523E-4	0.0	0.0	0.0	5.980657357972844E-5
8	4.585170641112514E-4	0.0	0.0	0.0	5.980657357972844E-5
9	4.983881131644037E-4	0.0	0.0	0.0	5.980657357972844E-5
10	5.382591622175559E-4	0.0	0.0	0.0	5.980657357972844E-5
11	5.980657357972844E-4	0.0	0.0	0.0	5.980657357972844E-5
12	6.77807833903589E-4	0.0	0.0	0.0	5.980657357972844E-5
13	7.774854565364697E-4	0.0	0.0	0.0	5.980657357972844E-5
14	8.970986036959266E-4	0.0	0.0	0.0	5.980657357972844E-5
15	9.768407018022312E-4	0.0	0.0	0.0	5.980657357972844E-5
16	0.0010167117508553835	0.0	0.0	0.0	9.967762263288073E-5
17	0.0010964538489616881	0.0	0.0	0.0	1.1961314715945688E-4
18	0.0011562604225414165	0.0	0.0	0.0	1.1961314715945688E-4
19	0.0011761959470679927	1.9935524526576146E-5	0.0	0.0	1.1961314715945688E-4
20	0.001236002520647721	1.9935524526576146E-5	0.0	1.9935524526576146E-5	1.1961314715945688E-4
21	0.0012958090942274496	1.9935524526576146E-5	0.0	1.9935524526576146E-5	1.1961314715945688E-4
22	0.001335680143280602	1.9935524526576146E-5	0.0	3.987104905315229E-5	1.1961314715945688E-4
23	0.0013755511923337542	1.9935524526576146E-5	0.0	3.987104905315229E-5	1.1961314715945688E-4
24	0.0013755511923337542	1.9935524526576146E-5	0.0	7.974209810630458E-5	1.1961314715945688E-4
25	0.0014353577659134825	1.9935524526576146E-5	0.0	1.3954867168603302E-4	1.1961314715945688E-4
26	0.0014552932904400588	1.9935524526576146E-5	0.0	1.3954867168603302E-4	1.1961314715945688E-4
27	0.001495164339493211	1.9935524526576146E-5	0.0	1.5948419621260917E-4	1.1961314715945688E-4
28	0.0015350353885463634	1.9935524526576146E-5	0.0	1.5948419621260917E-4	1.1961314715945688E-4
29	0.0015350353885463634	1.9935524526576146E-5	0.0	1.5948419621260917E-4	1.1961314715945688E-4
30	0.0015549709130729394	1.9935524526576146E-5	0.0	1.5948419621260917E-4	1.3954867168603302E-4
31	0.0015948419621260917	1.9935524526576146E-5	0.0	1.5948419621260917E-4	1.3954867168603302E-4
32	0.0016546485357058203	1.9935524526576146E-5	0.0	1.5948419621260917E-4	1.3954867168603302E-4
33	0.001754326158338701	1.9935524526576146E-5	0.0	1.5948419621260917E-4	1.3954867168603302E-4
34	0.0017742616828652772	1.9935524526576146E-5	0.0	1.5948419621260917E-4	1.3954867168603302E-4
35	0.0018141327319184295	1.9935524526576146E-5	0.0	1.5948419621260917E-4	1.3954867168603302E-4
36	0.0019138103545513101	1.9935524526576146E-5	0.0	1.7941972073918532E-4	1.5948419621260917E-4
37	0.0019138103545513101	1.9935524526576146E-5	0.0	1.7941972073918532E-4	1.5948419621260917E-4
38	0.0019536814036044624	1.9935524526576146E-5	0.0	1.7941972073918532E-4	1.5948419621260917E-4
39	0.0020134879771841908	1.9935524526576146E-5	0.0	1.9935524526576147E-4	1.5948419621260917E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	10845	0.0	35.72614	1
TCAACGC	12235	0.0	31.906418	12
CAACGCA	12305	0.0	31.816336	13
ATCAACG	12380	0.0	31.587236	11
ACGCAGA	12495	0.0	31.206482	15
AACGCAG	12540	0.0	31.202154	14
GGTATCA	12680	0.0	30.82216	8
CGCAGAG	12695	0.0	30.768017	16
GTGGTAT	12785	0.0	30.51623	6
GTATCAA	12855	0.0	30.437574	9
TGGTATC	12870	0.0	30.419579	7
AGTGGTA	12880	0.0	30.413433	5
TATCAAC	12950	0.0	30.16216	10
AGCAGTG	13000	0.0	30.08077	2
AGAGTAC	12945	0.0	29.756662	19
GCAGTGG	13435	0.0	29.24079	3
CAGAGTA	13390	0.0	28.918968	18
GCAGAGT	13600	0.0	28.621325	17
CAGTGGT	14125	0.0	27.876108	4
GAGTACT	6735	0.0	27.66147	20
>>END_MODULE