FastQCFastQC Report
Thu 26 May 2016
SRR805430_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805430_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12840266
Sequences flagged as poor quality0
Sequence length53
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT264770.20620289330454683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT172200.031.2241571
TCAACGC220400.024.5022712
CAACGCA220700.024.4263713
AACGCAG226950.023.78475414
ATCAACG229300.023.56149111
ACGCAGA228950.023.55645215
AGCAGTG232650.023.0303042
AGTGGTA232350.022.9184425
CGCAGAG237750.022.73396516
GTATCAA236850.022.66169
GGTATCA237050.022.5433468
GTGGTAT236600.022.5067626
TGGTATC237450.022.475687
TATCAAC242700.022.13473510
GCAGTGG245000.021.9653073
AGAGTAC205400.021.58933819
CAGTGGT255850.020.9786994
GAGTACT88650.020.75634420
ACATGGG149150.020.6245424
GTACTTT94950.019.00789822
Produced by FastQC (version 0.11.5)