##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805430_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12840266
Sequences flagged as poor quality	0
Sequence length	53
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.54707519299055	37.0	35.0	37.0	33.0	37.0
2	35.620923040067865	37.0	35.0	37.0	33.0	37.0
3	37.435649853359735	39.0	37.0	39.0	35.0	39.0
4	37.512077631413554	39.0	37.0	39.0	35.0	39.0
5	37.48138558811788	39.0	37.0	39.0	35.0	39.0
6	37.35606365164086	39.0	37.0	39.0	34.0	39.0
7	37.37305613450687	39.0	37.0	39.0	34.0	39.0
8	38.21140239618089	40.0	38.0	40.0	34.0	40.0
9	38.26224970728799	40.0	38.0	40.0	34.0	40.0
10	38.34590233566812	40.0	38.0	40.0	35.0	40.0
11	38.35367468244038	40.0	38.0	40.0	35.0	40.0
12	38.3852406951694	40.0	38.0	40.0	35.0	40.0
13	38.42001980332806	40.0	38.0	40.0	35.0	40.0
14	38.35538757530413	40.0	38.0	40.0	34.0	40.0
15	38.34798983136331	40.0	38.0	40.0	34.0	40.0
16	38.329909598446015	40.0	38.0	40.0	34.0	40.0
17	38.24577029790505	40.0	38.0	40.0	34.0	40.0
18	38.19399691564022	40.0	38.0	40.0	34.0	40.0
19	38.15756628406296	40.0	38.0	40.0	34.0	40.0
20	38.04303649161162	40.0	38.0	40.0	34.0	40.0
21	38.07271811970251	40.0	38.0	40.0	34.0	40.0
22	38.012748879189886	40.0	38.0	40.0	34.0	40.0
23	32.49889122234695	40.0	34.0	40.0	3.0	40.0
24	24.210047439827182	32.0	3.0	40.0	3.0	40.0
25	29.970603646373057	34.0	17.0	40.0	16.0	40.0
26	34.34597982627463	37.0	30.0	40.0	25.0	40.0
27	36.334029528671756	38.0	35.0	40.0	30.0	40.0
28	36.569607903761494	39.0	36.0	40.0	30.0	40.0
29	37.06381768103558	39.0	37.0	40.0	31.0	40.0
30	37.364522121270696	40.0	37.0	40.0	32.0	40.0
31	37.47399524277768	40.0	37.0	40.0	33.0	40.0
32	37.49431686228307	40.0	37.0	40.0	33.0	40.0
33	37.557211587361195	40.0	38.0	40.0	33.0	40.0
34	37.56910113855897	40.0	38.0	40.0	33.0	40.0
35	37.30125995832174	40.0	37.0	40.0	32.0	40.0
36	37.469646111692704	40.0	37.0	40.0	33.0	40.0
37	37.35136725360674	40.0	37.0	40.0	32.0	40.0
38	37.32327741496944	40.0	37.0	40.0	32.0	40.0
39	37.312106774112	40.0	37.0	40.0	32.0	40.0
40	37.32095550045459	40.0	37.0	40.0	32.0	40.0
41	37.265458363557265	40.0	37.0	40.0	32.0	40.0
42	37.20286994054484	40.0	37.0	40.0	32.0	40.0
43	37.232152199962215	40.0	37.0	40.0	32.0	40.0
44	37.06301761972844	40.0	37.0	40.0	31.0	40.0
45	37.05577267636044	40.0	36.0	40.0	31.0	40.0
46	36.923096842386286	40.0	36.0	40.0	31.0	40.0
47	36.7021877895676	39.0	36.0	40.0	31.0	40.0
48	36.70871787235561	39.0	36.0	40.0	31.0	40.0
49	36.60379333263034	39.0	35.0	40.0	30.0	40.0
50	36.51613315487389	39.0	35.0	40.0	30.0	40.0
51	36.432032327056156	39.0	35.0	40.0	30.0	40.0
52	36.236200091181914	39.0	35.0	40.0	30.0	40.0
53	35.87106980494018	38.0	35.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	13.0
13	39.0
14	175.0
15	443.0
16	987.0
17	1880.0
18	3447.0
19	6012.0
20	9747.0
21	15084.0
22	22460.0
23	32474.0
24	45138.0
25	61328.0
26	80990.0
27	104980.0
28	133705.0
29	168992.0
30	212043.0
31	263350.0
32	329080.0
33	415749.0
34	541089.0
35	738172.0
36	1110782.0
37	2196095.0
38	3151752.0
39	3194258.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.35007171969802	29.835363223783684	22.855009389992386	25.95955566652591
2	24.25283089929757	24.37177703327953	29.769912866291087	21.605479201131814
3	20.739282192440562	26.39664941520682	27.627675314514512	25.23639307783811
4	21.305765783979865	28.75739490132058	27.257776435472596	22.679062879226958
5	24.807710369863052	28.39646001103092	25.74431090446257	21.05151871464345
6	24.019424519710107	26.449179479615143	27.655011196808537	21.876384803866213
7	23.35750676816197	27.28431015369931	27.589701023327713	21.768482054811013
8	22.713672754131416	26.32730505738744	27.960293034427792	22.99872915405335
9	22.499689648173955	26.272586564795464	28.703462996794617	22.524260790235964
10	22.126636628867345	27.089828201378385	28.317520836406352	22.46601433334792
11	22.089729293770084	27.848636469057574	27.565737345316677	22.49589689185567
12	22.12494663272552	26.910961190367864	27.978322255940803	22.98576992096581
13	22.368212621140405	27.30544678747309	26.783790927695733	23.542549663690767
14	22.59376090806842	27.931056879974292	26.81250528610544	22.662676925851848
15	22.577779930727292	27.512000140807054	26.75953909366052	23.150680834805133
16	22.94991396595678	26.866499494636635	26.550182060091277	23.633404479315303
17	23.855463741950516	26.707702161310365	26.56258055713176	22.874253539607356
18	22.780509375740348	26.94534521325337	26.524395989927314	23.749749421078974
19	23.292414658699435	27.6071305687904	26.413720712639442	22.686734059870723
20	23.831788219963666	26.781298767486593	26.674533066526816	22.712379946022924
21	23.090347193741938	27.45774892825429	26.646309352158283	22.805594525845493
22	23.712491625952296	26.651270308574603	26.8037905133741	22.832447552098998
23	19.51554586174461	37.474169148832274	23.570493010035772	19.439791979387344
24	15.973056944458937	49.05353206857241	18.72970544379688	16.243705543171767
25	22.92309209170589	26.709236397439117	26.71548237396328	23.652189136891714
26	23.071344472147228	26.951887133802366	27.362003248219313	22.614765145831093
27	23.212058067955912	26.32584091326457	27.608166372877324	22.853934645902196
28	24.04038202946886	26.414608544713946	26.882075495943774	22.662933929873414
29	23.719820134567303	26.5154242131744	27.02605226402631	22.738703388231986
30	23.533570098937204	26.459833464509224	27.022757939749848	22.983838496803727
31	23.38236606624816	26.69391740015355	26.942424713008283	22.98129182059001
32	23.234697785855836	26.774733482935638	27.084353236918922	22.906215494289604
33	23.175228612865183	26.60535225672116	27.05366072634321	23.165758404070445
34	23.2829444499047	26.72915810311095	26.928780135863228	23.059117311121124
35	23.524769658198668	26.629923398783173	27.031939992520403	22.813366950497755
36	23.161264727693336	26.61377108542767	27.021667619658345	23.20329656722065
37	23.324618041401944	26.748822804761208	26.855082285678506	23.07147686815834
38	23.30758568397259	26.83844711628248	26.950968149725245	22.90299905001968
39	23.23457317784538	26.650507084510554	26.99394233733164	23.120977400312423
40	23.2999690193334	26.739523931980848	26.86938884287911	23.09111820580664
41	23.23925376623818	26.740365036051433	27.059898914866714	22.96048228284367
42	23.20321089921346	26.6224547061564	27.05472768243275	23.119606712197395
43	23.302912883580447	26.69799831249602	26.864723830487623	23.134364973435908
44	23.26965812078971	26.663528621603323	27.042656281419713	23.024156976187253
45	23.221442608743466	26.51322799699009	27.059244722811815	23.206084671454626
46	23.312671248399372	26.688559255703893	26.885463276228077	23.113306219668658
47	23.316362760709165	26.685272719428088	26.96603014298925	23.0323343768735
48	23.22045353266046	26.593732559746037	26.95025165366512	23.235562253928386
49	23.32654946556403	26.733114407442958	26.802746921286523	23.137589205706487
50	23.359282432310984	26.664774701707895	26.981979968327757	22.99396289765337
51	23.262212792164895	26.61543771756753	26.914652702677657	23.207696787589917
52	23.361953716535155	26.753106205120673	26.726884006920105	23.158056071424067
53	23.331946550016955	26.756221405382103	26.819631306703464	23.092200737897485
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8602.0
1	5395.0
2	2188.0
3	1763.5
4	1339.0
5	1346.5
6	1354.0
7	1507.5
8	2083.5
9	2506.0
10	3506.0
11	4506.0
12	6292.5
13	8079.0
14	10767.0
15	13455.0
16	19052.0
17	24649.0
18	33560.5
19	42472.0
20	58701.5
21	74931.0
22	111838.5
23	148746.0
24	152206.5
25	180432.5
26	205198.0
27	232144.5
28	259091.0
29	284928.5
30	310766.0
31	337296.5
32	363827.0
33	400174.0
34	436521.0
35	477237.5
36	517954.0
37	560243.0
38	602532.0
39	639715.0
40	676898.0
41	714882.0
42	784445.5
43	816025.0
44	841085.0
45	866145.0
46	881652.0
47	897159.0
48	897894.0
49	898629.0
50	891414.0
51	884199.0
52	860769.0
53	837339.0
54	794248.0
55	751157.0
56	697584.0
57	644011.0
58	580289.5
59	453132.5
60	389697.0
61	335943.5
62	282190.0
63	235412.0
64	188634.0
65	153091.5
66	117549.0
67	92608.0
68	67667.0
69	51655.0
70	35643.0
71	26347.0
72	17051.0
73	12530.0
74	8009.0
75	5894.5
76	2711.5
77	1643.0
78	1194.5
79	746.0
80	569.0
81	392.0
82	267.5
83	143.0
84	95.0
85	47.0
86	34.0
87	21.0
88	14.5
89	8.0
90	4.5
91	1.0
92	3.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1.2840266E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.05287614800787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.10783691334989	55.876744562450284
2	10.927985083575106	14.873616308796278
3	3.002910148060796	6.130700171687319
4	1.2796875686203009	3.4834567846185065
5	0.6982170545660525	2.375783936940521
6	0.4254585477033882	1.7372206711781981
7	0.2959456874226137	1.4097968648895725
8	0.21998917725303394	1.1976716986802294
9	0.17104180356192492	1.0475898006538409
>10	0.8367739568632779	9.64425121340266
>50	0.029258122512266706	1.3117565953981023
>100	0.0047340335784196675	0.4640599667655801
>500	5.7866077615683335E-5	0.0349559165459057
>1k	6.93594342044534E-5	0.10059788701246464
>5k	2.3118280641009036E-5	0.10352086919050991
>10k+	1.1559140320504518E-5	0.20827675179005556
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26477	0.20620289330454683	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0903200914996621E-4	1.557600130713803E-5	0.0	0.0	1.557600130713803E-5
2	1.0903200914996621E-4	1.557600130713803E-5	0.0	0.0	1.557600130713803E-5
3	1.0903200914996621E-4	1.557600130713803E-5	0.0	0.0	1.557600130713803E-5
4	1.0903200914996621E-4	1.557600130713803E-5	0.0	0.0	2.3364001960707044E-5
5	1.0903200914996621E-4	1.557600130713803E-5	0.0	0.0	3.115200261427606E-5
6	1.0903200914996621E-4	1.557600130713803E-5	0.0	0.0	5.4516004574983104E-5
7	1.0903200914996621E-4	1.557600130713803E-5	0.0	0.0	5.4516004574983104E-5
8	1.0903200914996621E-4	1.557600130713803E-5	0.0	7.788000653569015E-6	6.230400522855212E-5
9	1.0903200914996621E-4	1.557600130713803E-5	0.0	7.788000653569015E-6	6.230400522855212E-5
10	1.1682000980353522E-4	1.557600130713803E-5	0.0	7.788000653569015E-6	7.788000653569015E-5
11	1.1682000980353522E-4	1.557600130713803E-5	0.0	7.788000653569015E-6	7.788000653569015E-5
12	1.1682000980353522E-4	1.557600130713803E-5	0.0	7.009200588212113E-5	7.788000653569015E-5
13	1.1682000980353522E-4	1.557600130713803E-5	0.0	1.8691201568565635E-4	8.566800718925916E-5
14	1.2460801045710424E-4	1.557600130713803E-5	0.0	2.6479202222134653E-4	8.566800718925916E-5
15	1.2460801045710424E-4	1.557600130713803E-5	0.0	4.2834003594629583E-4	8.566800718925916E-5
16	1.2460801045710424E-4	1.557600130713803E-5	0.0	4.2834003594629583E-4	8.566800718925916E-5
17	1.3239601111067326E-4	1.557600130713803E-5	0.0	4.2834003594629583E-4	8.566800718925916E-5
18	1.4018401176424226E-4	1.557600130713803E-5	0.0	4.2834003594629583E-4	9.345600784282817E-5
19	1.557600130713803E-4	3.115200261427606E-5	0.0	4.2834003594629583E-4	9.345600784282817E-5
20	1.557600130713803E-4	3.894000326784508E-5	0.0	4.2834003594629583E-4	9.345600784282817E-5
21	1.8691201568565635E-4	3.894000326784508E-5	0.0	4.3612803659986483E-4	9.345600784282817E-5
22	2.024880169927944E-4	3.894000326784508E-5	0.0	4.3612803659986483E-4	9.345600784282817E-5
23	2.102760176463634E-4	3.894000326784508E-5	0.0	4.3612803659986483E-4	1.012440084963972E-4
24	2.1806401829993242E-4	3.894000326784508E-5	0.0	4.4391603725343383E-4	1.012440084963972E-4
25	2.1806401829993242E-4	3.894000326784508E-5	0.0	4.4391603725343383E-4	1.0903200914996621E-4
26	2.3364001960707044E-4	3.894000326784508E-5	0.0	4.517040379070029E-4	1.1682000980353522E-4
27	2.492160209142085E-4	3.894000326784508E-5	0.0	4.517040379070029E-4	1.1682000980353522E-4
28	2.6479202222134653E-4	3.894000326784508E-5	0.0	4.517040379070029E-4	1.1682000980353522E-4
29	3.193080267963296E-4	3.894000326784508E-5	0.0	4.517040379070029E-4	1.1682000980353522E-4
30	3.3488402810346766E-4	3.894000326784508E-5	0.0	4.517040379070029E-4	1.1682000980353522E-4
31	3.660360307177437E-4	3.894000326784508E-5	0.0	4.517040379070029E-4	1.1682000980353522E-4
32	4.6728003921414087E-4	3.894000326784508E-5	0.0	4.517040379070029E-4	1.4018401176424226E-4
33	5.763120483641071E-4	3.894000326784508E-5	0.0	4.517040379070029E-4	1.4797201241781128E-4
34	6.541920548997972E-4	3.894000326784508E-5	0.0	4.517040379070029E-4	1.4797201241781128E-4
35	7.009200588212113E-4	3.894000326784508E-5	0.0	4.517040379070029E-4	1.4797201241781128E-4
36	7.554360633961944E-4	3.894000326784508E-5	0.0	4.517040379070029E-4	1.557600130713803E-4
37	8.177400686247466E-4	3.894000326784508E-5	0.0	4.517040379070029E-4	1.7133601437851833E-4
38	8.488920712390226E-4	3.894000326784508E-5	0.0	4.517040379070029E-4	1.7133601437851833E-4
39	9.111960764675748E-4	3.894000326784508E-5	7.788000653569015E-6	4.517040379070029E-4	1.7133601437851833E-4
40	9.657120810425578E-4	3.894000326784508E-5	7.788000653569015E-6	4.517040379070029E-4	1.7912401503208735E-4
41	0.001066956089538955	3.894000326784508E-5	7.788000653569015E-6	4.517040379070029E-4	1.8691201568565635E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	17220	0.0	31.224157	1
TCAACGC	22040	0.0	24.50227	12
CAACGCA	22070	0.0	24.42637	13
AACGCAG	22695	0.0	23.784754	14
ATCAACG	22930	0.0	23.561491	11
ACGCAGA	22895	0.0	23.556452	15
AGCAGTG	23265	0.0	23.030304	2
AGTGGTA	23235	0.0	22.918442	5
CGCAGAG	23775	0.0	22.733965	16
GTATCAA	23685	0.0	22.6616	9
GGTATCA	23705	0.0	22.543346	8
GTGGTAT	23660	0.0	22.506762	6
TGGTATC	23745	0.0	22.47568	7
TATCAAC	24270	0.0	22.134735	10
GCAGTGG	24500	0.0	21.965307	3
AGAGTAC	20540	0.0	21.589338	19
CAGTGGT	25585	0.0	20.978699	4
GAGTACT	8865	0.0	20.756344	20
ACATGGG	14915	0.0	20.62454	24
GTACTTT	9495	0.0	19.007898	22
>>END_MODULE