FastQCFastQC Report
Thu 26 May 2016
SRR805429_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805429_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086428
Sequences flagged as poor quality0
Sequence length53
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT318612.9326379658845316No Hit
AAGCAGTGGTATCAACGCAGAGAACTTTTTTTTTTTTTTTTTTTTTTTTTTTT64220.591111422017842No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18260.16807372416763927No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG17630.1622749045495882No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG13650.1256410917244401No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA12790.11772524272202116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT116100.044.8139531
AGTGGTA120300.043.1126375
CAACGCA121350.043.1075413
TCAACGC121300.043.10593812
GTGGTAT120400.043.0963446
TGGTATC120500.043.0605857
ATCAACG121600.043.01891311
GCAGTGG121050.042.9231723
GGTATCA121650.042.9046448
AACGCAG122400.042.77614614
GTATCAA122300.042.7534759
CGCAGAG122150.042.7482616
AGCAGTG121750.042.734292
CAGTGGT121400.042.7219924
ACGCAGA122400.042.6993515
TATCAAC123050.042.47379310
AGAGTAC101850.042.17771519
GAGTACT51650.042.0861620
ACATGGG58200.041.9931324
GTACTTT51200.041.49218822