##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805429_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1086428
Sequences flagged as poor quality	0
Sequence length	53
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.249216699127786	33.0	32.0	35.0	28.0	35.0
2	33.998916633223736	35.0	35.0	35.0	31.0	37.0
3	36.81969997091386	39.0	37.0	39.0	33.0	39.0
4	37.05016347148638	39.0	37.0	39.0	33.0	39.0
5	37.09895179432047	39.0	37.0	39.0	34.0	39.0
6	37.16995603942461	39.0	37.0	39.0	34.0	39.0
7	37.077639751552795	39.0	37.0	39.0	34.0	39.0
8	38.039083123778106	40.0	38.0	40.0	34.0	40.0
9	38.187979323066045	40.0	38.0	40.0	34.0	40.0
10	37.84140044255118	40.0	37.0	40.0	33.0	40.0
11	38.097124705917004	40.0	38.0	40.0	34.0	40.0
12	38.2446982220635	40.0	38.0	40.0	34.0	40.0
13	38.31204368812291	40.0	38.0	40.0	34.0	40.0
14	38.27509508223279	40.0	38.0	40.0	34.0	40.0
15	38.28227549363603	40.0	38.0	40.0	34.0	40.0
16	38.19693895959971	40.0	38.0	40.0	34.0	40.0
17	38.12338691565387	40.0	38.0	40.0	34.0	40.0
18	38.065939942637705	40.0	38.0	40.0	34.0	40.0
19	38.00601420434672	40.0	38.0	40.0	34.0	40.0
20	37.88256561870644	40.0	38.0	40.0	34.0	40.0
21	37.84150997581064	40.0	38.0	40.0	34.0	40.0
22	37.7957333573877	40.0	38.0	40.0	34.0	40.0
23	31.713702150533674	38.0	31.0	40.0	3.0	40.0
24	24.227942394710002	32.0	3.0	40.0	3.0	40.0
25	29.84824765193828	34.0	16.0	40.0	16.0	40.0
26	34.020548071294186	37.0	30.0	40.0	25.0	40.0
27	36.058756769891794	38.0	35.0	40.0	30.0	40.0
28	36.186127382578505	38.0	35.0	40.0	30.0	40.0
29	36.778468522534396	39.0	36.0	40.0	30.0	40.0
30	37.14284057480109	40.0	37.0	40.0	31.0	40.0
31	37.277739528068125	40.0	37.0	40.0	32.0	40.0
32	37.303615149830456	40.0	37.0	40.0	32.0	40.0
33	37.30307944935145	40.0	37.0	40.0	32.0	40.0
34	37.32863935760124	40.0	37.0	40.0	32.0	40.0
35	37.145670030595674	40.0	37.0	40.0	31.0	40.0
36	37.17048161498047	40.0	37.0	40.0	32.0	40.0
37	36.90656812968738	40.0	35.0	40.0	31.0	40.0
38	36.84482174612584	40.0	35.0	40.0	31.0	40.0
39	36.81707485447724	40.0	35.0	40.0	31.0	40.0
40	36.779133085671575	40.0	35.0	40.0	31.0	40.0
41	36.71111293155184	40.0	35.0	40.0	31.0	40.0
42	36.66096878946419	39.0	35.0	40.0	31.0	40.0
43	36.625235174351175	39.0	35.0	40.0	31.0	40.0
44	36.49856594270398	39.0	35.0	40.0	30.0	40.0
45	36.449229953572626	39.0	35.0	40.0	30.0	40.0
46	36.338947449807996	39.0	35.0	40.0	30.0	40.0
47	36.14477535556889	39.0	35.0	40.0	30.0	40.0
48	36.09501780145578	39.0	35.0	40.0	29.0	40.0
49	35.89588909711458	38.0	35.0	40.0	28.0	40.0
50	35.80742948451255	38.0	35.0	40.0	28.0	40.0
51	35.68128951021144	38.0	34.0	40.0	28.0	40.0
52	35.341706951588144	38.0	34.0	40.0	27.0	40.0
53	34.881186788263925	37.0	34.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	5.0
13	11.0
14	31.0
15	62.0
16	136.0
17	214.0
18	392.0
19	676.0
20	1024.0
21	1650.0
22	2310.0
23	3338.0
24	4698.0
25	6397.0
26	8156.0
27	10419.0
28	13483.0
29	16627.0
30	20862.0
31	26324.0
32	32814.0
33	42662.0
34	56245.0
35	79640.0
36	116579.0
37	214477.0
38	215929.0
39	211263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	10.194324888533801	46.735448644548924	26.9968189332381	16.073407533679177
2	25.931584973877698	44.46221930951706	16.919943153158794	12.686252563446452
3	35.40050514162006	25.5369891055827	22.20009057203975	16.86241518075749
4	20.698104246208676	26.08161792590029	21.67506728471652	31.545210543174512
5	18.362192432448353	37.607830431469	24.870953252309402	19.159023883773248
6	30.37016719009451	24.909980228786445	23.701340539824084	21.018512041294958
7	20.864245030503632	25.053201868876723	34.45575776765695	19.6267953329627
8	28.79159962740283	25.868626360881713	25.57978991704927	19.75998409466619
9	29.83253377122092	24.994017090870265	25.856660542622244	19.31678859528657
10	19.84227210638901	26.005588957574734	34.88505450890441	19.26708442713185
11	19.22999039052749	35.97063035930591	25.487192892672134	19.31218635749447
12	19.407084500767656	25.621670280957414	35.31177399698829	19.65947122128664
13	19.341732724119776	26.235608802424093	25.082380056478666	29.340278416977473
14	19.60037848803602	36.29637675023103	24.610650682788	19.492594078944947
15	19.399720920300286	35.85851984669026	24.737120177315017	20.00463905569444
16	19.95033264974761	25.65996089938772	24.7355554164657	29.654151034398968
17	29.93755683763673	25.329980449693863	24.905009811970974	19.827452900698436
18	19.813278008298756	25.483879281461817	24.697632977058763	30.00520973318066
19	20.175105943513973	35.7772443272817	24.50185378138266	19.545795947821667
20	30.23384890669239	25.921552095490913	24.591413328817005	19.253185668999695
21	20.378248719657446	35.51574517593435	24.734174745128072	19.371831359280137
22	29.98606442396551	25.525851690125805	25.08081529562935	19.407268590279337
23	17.106241738983165	36.53348404128023	29.658201003656014	16.70207321608059
24	14.178758279425788	53.86256613415708	17.617734447197606	14.340941139219535
25	19.746545560313248	25.589638705924365	24.99659434403384	29.667221389728542
26	20.05535571616343	30.26560434745791	30.216820626861608	19.46221930951706
27	20.3718055867485	24.889086069210293	35.05515321770057	19.68395512634063
28	25.388705003921107	25.249257198820352	29.88205384986396	19.47998394739458
29	25.186390630580213	25.176541841705113	29.930009167657683	19.707058360056994
30	25.12564109172444	25.05218937656246	30.106090785583582	19.71607874612952
31	23.08970313725346	26.056397662799558	30.39483518466019	20.459064015286792
32	22.287441045333882	26.47759446553292	30.923264127949572	20.31170036118362
33	22.12884793101798	26.04894203757635	31.05590062111801	20.766309410287658
34	21.626651743143587	26.273991465610237	31.13459888736299	20.964757903883186
35	21.444034947552897	26.23201905694625	31.31896453331468	21.004981462186173
36	21.430780502711638	26.176700158685158	31.452889653064908	20.939629685538296
37	21.75293714815892	26.26828469074803	31.115821757171208	20.862956403921842
38	21.69320010161741	26.32047406731049	31.438070447374333	20.548255383697768
39	21.59498834713391	26.38959967894789	31.41100928915676	20.60440268476144
40	21.465113196640733	26.638396653989037	31.030864447528966	20.865625701841264
41	21.570872621103284	26.972887296719154	30.92897090281178	20.527269179365774
42	21.664113958771313	26.457252574491818	31.108182042436315	20.77045142430055
43	21.48609940097273	26.745904928812585	31.266591067240533	20.501404602974148
44	21.46492910712905	26.604063960059936	31.251587772038274	20.67941916077273
45	21.75910414680034	26.059159005474825	31.223053897727233	20.958682949997606
46	21.685836521150044	26.319553619752067	30.85956915690685	21.135040702191034
47	21.463180256768048	26.277765300599764	31.051942696616802	21.207111746015382
48	21.254422750518213	26.089717864414393	31.35523016711646	21.300629217950938
49	21.150043997393293	26.76459001424853	30.73853030297452	21.34683568538366
50	21.642391396392583	26.774622892635314	30.787498113082506	20.795487597889597
51	21.740234971852715	26.482104658569185	30.839227265865755	20.93843310371235
52	21.800524286929278	26.559698387744056	30.537228421947887	21.10254890337878
53	22.022628282776218	26.802052229876256	30.135268973185518	21.040050514162008
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2042.0
1	1213.0
2	384.0
3	307.0
4	230.0
5	225.5
6	221.0
7	255.0
8	308.5
9	328.0
10	464.0
11	600.0
12	839.0
13	1078.0
14	1387.0
15	1696.0
16	2283.5
17	2871.0
18	3985.0
19	5099.0
20	6951.5
21	8804.0
22	31944.5
23	55085.0
24	39564.5
25	24485.5
26	24927.0
27	26860.0
28	28793.0
29	30876.5
30	32960.0
31	34570.0
32	36180.0
33	39227.5
34	42275.0
35	45629.0
36	48983.0
37	51829.0
38	54675.0
39	56958.5
40	59242.0
41	61531.5
42	64903.0
43	65985.0
44	67291.5
45	68598.0
46	68680.5
47	68763.0
48	68277.5
49	67792.0
50	66232.0
51	64672.0
52	62154.5
53	59637.0
54	55618.0
55	51599.0
56	48960.5
57	46322.0
58	40155.0
59	28606.0
60	23224.0
61	19819.0
62	16414.0
63	13671.0
64	10928.0
65	8647.5
66	6367.0
67	5038.0
68	3709.0
69	2841.0
70	1973.0
71	1441.5
72	910.0
73	688.5
74	467.0
75	352.5
76	178.0
77	118.0
78	81.5
79	45.0
80	39.0
81	33.0
82	21.0
83	9.0
84	5.5
85	2.0
86	2.5
87	3.0
88	3.0
89	3.0
90	2.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	1086428.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.98770167095546
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.8217527512559	79.73695133069882
2	4.280383287442779	7.27559875741061
3	0.9199175258005737	2.345450287338679
4	0.39614604069737325	1.3467016609967415
5	0.18645071131546503	0.792300871480809
6	0.11439053488321689	0.5833073191581516
7	0.07728257800660761	0.45976480787915386
8	0.04615926955595755	0.31383761842967495
9	0.033681546612718216	0.2576265511804269
>10	0.11454610622537054	1.4958252977176756
>50	0.004000397727120722	0.23580743902181667
>100	0.004101869306383849	0.7417097255767396
>500	4.317749711096192E-4	0.23411768832668528
>1k	5.397187138870239E-4	0.6689601031654345
>5k	1.079437427774048E-4	0.5891472548722464
>10k+	1.079437427774048E-4	2.922893286746285
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31861	2.9326379658845316	No Hit
AAGCAGTGGTATCAACGCAGAGAACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6422	0.591111422017842	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1826	0.16807372416763927	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCAGTG	1763	0.1622749045495882	No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGG	1365	0.1256410917244401	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1279	0.11772524272202116	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.7613426752624197E-4	0.0	0.0	0.0	0.0
3	2.7613426752624197E-4	0.0	0.0	0.0	0.0
4	2.7613426752624197E-4	0.0	0.0	0.0	0.0
5	2.7613426752624197E-4	0.0	0.0	0.0	0.0
6	3.6817902336832263E-4	0.0	0.0	0.0	0.0
7	3.6817902336832263E-4	9.204475584208066E-5	0.0	0.0	0.0
8	3.6817902336832263E-4	9.204475584208066E-5	0.0	0.0	0.0
9	3.6817902336832263E-4	9.204475584208066E-5	0.0	0.0	0.0
10	3.6817902336832263E-4	9.204475584208066E-5	0.0	0.0	0.0
11	3.6817902336832263E-4	9.204475584208066E-5	0.0	0.0	0.0
12	3.6817902336832263E-4	9.204475584208066E-5	0.0	9.204475584208066E-5	0.0
13	3.6817902336832263E-4	9.204475584208066E-5	0.0	9.204475584208066E-5	0.0
14	3.6817902336832263E-4	9.204475584208066E-5	0.0	9.204475584208066E-5	0.0
15	3.6817902336832263E-4	9.204475584208066E-5	0.0	3.6817902336832263E-4	0.0
16	3.6817902336832263E-4	9.204475584208066E-5	0.0	3.6817902336832263E-4	0.0
17	3.6817902336832263E-4	9.204475584208066E-5	0.0	3.6817902336832263E-4	0.0
18	3.6817902336832263E-4	9.204475584208066E-5	0.0	3.6817902336832263E-4	0.0
19	3.6817902336832263E-4	9.204475584208066E-5	0.0	3.6817902336832263E-4	0.0
20	3.6817902336832263E-4	9.204475584208066E-5	0.0	3.6817902336832263E-4	0.0
21	3.6817902336832263E-4	9.204475584208066E-5	0.0	3.6817902336832263E-4	0.0
22	3.6817902336832263E-4	9.204475584208066E-5	0.0	3.6817902336832263E-4	0.0
23	4.602237792104033E-4	9.204475584208066E-5	0.0	3.6817902336832263E-4	0.0
24	4.602237792104033E-4	9.204475584208066E-5	0.0	3.6817902336832263E-4	0.0
25	4.602237792104033E-4	9.204475584208066E-5	0.0	3.6817902336832263E-4	0.0
26	4.602237792104033E-4	9.204475584208066E-5	0.0	3.6817902336832263E-4	9.204475584208066E-5
27	4.602237792104033E-4	9.204475584208066E-5	0.0	3.6817902336832263E-4	9.204475584208066E-5
28	4.602237792104033E-4	9.204475584208066E-5	0.0	3.6817902336832263E-4	9.204475584208066E-5
29	4.602237792104033E-4	9.204475584208066E-5	0.0	3.6817902336832263E-4	9.204475584208066E-5
30	4.602237792104033E-4	9.204475584208066E-5	0.0	3.6817902336832263E-4	9.204475584208066E-5
31	5.522685350524839E-4	9.204475584208066E-5	0.0	3.6817902336832263E-4	9.204475584208066E-5
32	5.522685350524839E-4	9.204475584208066E-5	0.0	3.6817902336832263E-4	9.204475584208066E-5
33	5.522685350524839E-4	9.204475584208066E-5	0.0	3.6817902336832263E-4	9.204475584208066E-5
34	8.284028025787259E-4	9.204475584208066E-5	0.0	3.6817902336832263E-4	9.204475584208066E-5
35	0.0011965818259470485	9.204475584208066E-5	0.0	3.6817902336832263E-4	9.204475584208066E-5
36	0.0013806713376312099	9.204475584208066E-5	0.0	3.6817902336832263E-4	9.204475584208066E-5
37	0.0013806713376312099	9.204475584208066E-5	0.0	3.6817902336832263E-4	9.204475584208066E-5
38	0.0013806713376312099	9.204475584208066E-5	0.0	3.6817902336832263E-4	9.204475584208066E-5
39	0.0014727160934732905	9.204475584208066E-5	0.0	3.6817902336832263E-4	9.204475584208066E-5
40	0.0016568056051574518	9.204475584208066E-5	0.0	3.6817902336832263E-4	9.204475584208066E-5
41	0.0018408951168416132	9.204475584208066E-5	0.0	3.6817902336832263E-4	9.204475584208066E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	11610	0.0	44.813953	1
AGTGGTA	12030	0.0	43.112637	5
CAACGCA	12135	0.0	43.10754	13
TCAACGC	12130	0.0	43.105938	12
GTGGTAT	12040	0.0	43.096344	6
TGGTATC	12050	0.0	43.060585	7
ATCAACG	12160	0.0	43.018913	11
GCAGTGG	12105	0.0	42.923172	3
GGTATCA	12165	0.0	42.904644	8
AACGCAG	12240	0.0	42.776146	14
GTATCAA	12230	0.0	42.753475	9
CGCAGAG	12215	0.0	42.74826	16
AGCAGTG	12175	0.0	42.73429	2
CAGTGGT	12140	0.0	42.721992	4
ACGCAGA	12240	0.0	42.69935	15
TATCAAC	12305	0.0	42.473793	10
AGAGTAC	10185	0.0	42.177715	19
GAGTACT	5165	0.0	42.08616	20
ACATGGG	5820	0.0	41.99313	24
GTACTTT	5120	0.0	41.492188	22
>>END_MODULE