FastQCFastQC Report
Thu 26 May 2016
SRR805428_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR805428_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8864298
Sequences flagged as poor quality0
Sequence length53
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGAACTTTTTTTTTTTTTTTTTTTTTTTTTTTT302950.34176423220428737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT783200.044.0985031
TCAACGC796700.043.4279512
CAACGCA797150.043.4122813
ATCAACG800300.043.25609211
GTGGTAT794350.043.1600076
AACGCAG803050.043.08455314
GTATCAA799950.043.0370649
AGTGGTA797150.043.0349355
ACGCAGA803850.043.0299815
GGTATCA800850.042.9446838
TATCAAC803850.042.8662710
TGGTATC800250.042.8623547
CGCAGAG809200.042.75420416
AGAGTAC691200.042.5971519
GAGTACT328150.042.53847520
AGCAGTG817350.042.2818872
GCAGTGG819600.042.1400073
GTACTTT327950.042.09864422
CAGTGGT816800.042.0514224
CAGAGTA713250.041.4845418