##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR805428_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8864298
Sequences flagged as poor quality	0
Sequence length	53
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.20695705401601	35.0	35.0	37.0	33.0	37.0
2	35.57092067527513	37.0	35.0	37.0	33.0	37.0
3	37.403975588365824	39.0	37.0	39.0	34.0	39.0
4	37.473325016825925	39.0	37.0	39.0	35.0	39.0
5	37.411816818432776	39.0	37.0	39.0	34.0	39.0
6	37.33908167347262	39.0	37.0	39.0	34.0	39.0
7	37.28884938209433	39.0	37.0	39.0	34.0	39.0
8	38.19800812201937	40.0	38.0	40.0	34.0	40.0
9	38.242867737524165	40.0	38.0	40.0	34.0	40.0
10	38.00961474896263	40.0	38.0	40.0	34.0	40.0
11	38.18764430076696	40.0	38.0	40.0	34.0	40.0
12	38.316812002484575	40.0	38.0	40.0	34.0	40.0
13	38.37122928403355	40.0	38.0	40.0	34.0	40.0
14	38.34676361286591	40.0	38.0	40.0	34.0	40.0
15	38.334448368048996	40.0	38.0	40.0	34.0	40.0
16	38.271532838810245	40.0	38.0	40.0	34.0	40.0
17	38.21847922982734	40.0	38.0	40.0	34.0	40.0
18	38.13560972340957	40.0	38.0	40.0	34.0	40.0
19	38.04368467756838	40.0	38.0	40.0	34.0	40.0
20	37.96225296126101	40.0	38.0	40.0	34.0	40.0
21	37.91244958145586	40.0	38.0	40.0	34.0	40.0
22	37.88297550465925	40.0	38.0	40.0	34.0	40.0
23	33.070330329598576	39.0	34.0	40.0	3.0	40.0
24	24.586626149075766	34.0	3.0	40.0	3.0	40.0
25	29.963173507930353	35.0	16.0	40.0	16.0	40.0
26	34.126568398309715	37.0	30.0	40.0	25.0	40.0
27	36.17732458904247	38.0	35.0	40.0	30.0	40.0
28	36.7388330130598	39.0	36.0	40.0	30.0	40.0
29	37.03943177451841	39.0	37.0	40.0	31.0	40.0
30	37.29607962187192	40.0	37.0	40.0	32.0	40.0
31	37.28835594200466	40.0	37.0	40.0	31.0	40.0
32	37.351786120006345	40.0	37.0	40.0	32.0	40.0
33	37.37153229731221	40.0	37.0	40.0	32.0	40.0
34	37.38056301807543	40.0	37.0	40.0	32.0	40.0
35	37.0829726166697	40.0	37.0	40.0	31.0	40.0
36	37.14959582811859	40.0	37.0	40.0	31.0	40.0
37	36.95047165607474	40.0	36.0	40.0	31.0	40.0
38	36.979848488848184	40.0	36.0	40.0	31.0	40.0
39	36.90368284098752	40.0	36.0	40.0	31.0	40.0
40	36.86348304174792	40.0	36.0	40.0	31.0	40.0
41	36.795501685525466	40.0	36.0	40.0	31.0	40.0
42	36.73557827139837	39.0	35.0	40.0	31.0	40.0
43	36.75432098514739	39.0	35.0	40.0	31.0	40.0
44	36.602586352579756	39.0	35.0	40.0	31.0	40.0
45	36.53999414279619	39.0	35.0	40.0	30.0	40.0
46	36.38296772062492	39.0	35.0	40.0	30.0	40.0
47	36.21640991762687	39.0	35.0	40.0	30.0	40.0
48	36.126131928326416	39.0	35.0	40.0	29.0	40.0
49	35.979987473345325	39.0	35.0	40.0	29.0	40.0
50	35.81317200752953	38.0	35.0	40.0	28.0	40.0
51	35.63713686069669	38.0	34.0	40.0	28.0	40.0
52	35.36524821254881	38.0	34.0	40.0	28.0	40.0
53	34.96298962422067	37.0	34.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	16.0
12	27.0
13	66.0
14	197.0
15	417.0
16	860.0
17	1549.0
18	2913.0
19	5057.0
20	8444.0
21	12810.0
22	19237.0
23	27551.0
24	38185.0
25	50974.0
26	66083.0
27	84828.0
28	105678.0
29	131710.0
30	161012.0
31	198518.0
32	246238.0
33	311568.0
34	408639.0
35	566107.0
36	852082.0
37	1560792.0
38	1853899.0
39	2148834.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	11.856426758215935	38.839420786620664	25.723537272776703	23.580615182386694
2	27.368089385081596	41.79954238903069	14.63522548542479	16.197142740462922
3	33.701506876235435	26.111723680769757	19.680813979854918	20.50595546313989
4	21.645030435574256	24.635543615523755	20.861956581333345	32.85746936756864
5	20.38924007293076	33.41983764534992	24.2867850336259	21.90413724809342
6	30.024926959811143	24.030329305264782	22.781036919110797	23.163706815813274
7	22.868364759397757	23.651754487495793	31.37634813270041	22.10353262040604
8	29.290294617802786	25.074675964188025	24.12820507613801	21.506824341871177
9	29.76993778864384	24.3685850814131	24.320843004149907	21.540634125793154
10	21.565418942368588	25.063789597326263	31.814848733650425	21.555942726654724
11	21.308557090476874	33.200350439482065	24.04425031739682	21.446842152644237
12	21.165071390876076	24.754210654921575	32.193254333281665	21.887463620920684
13	21.23987708896971	25.064184439647676	23.940948284906487	29.75499018647613
14	21.697251152883172	33.34532525869505	23.645324198261385	21.312099390160395
15	21.509328770309843	32.94762879136058	23.62725170114994	21.915790737179638
16	21.81918974294411	24.506272239493754	23.77893883982691	29.895599177735228
17	30.075748807181345	24.388157979345912	23.751988031088306	21.784105182384437
18	21.626450284049568	24.58854609806665	23.78139814342884	30.003605474454943
19	21.95868189449407	32.72836720967639	23.669623922841946	21.643326972987595
20	30.200992791532954	24.666104411200976	23.82797825614617	21.30492454111989
21	22.055914636443855	32.53151010942999	23.803046783851357	21.609528470274803
22	29.784163393423825	24.38665757852455	24.107334839149136	21.721844188902494
23	19.251485001970828	33.699679320347755	28.13754681983841	18.911288857843
24	15.265314861932666	52.64904225918398	16.73226689806683	15.35337598081653
25	21.63723512002868	24.481758171938715	23.942516372982947	29.938490335049657
26	21.929847123821876	28.64820203472401	27.87528126874796	21.546669572706154
27	22.093356969722812	24.203112305114292	32.05003938270126	21.653491342461635
28	26.325310814234808	24.24631933628585	27.779402271900157	21.648967577579185
29	26.40390699861399	24.363689036627605	27.667267052619394	21.56513691213901
30	26.269220642176066	24.262643245973905	27.713305667295934	21.7548304445541
31	24.596679849887717	24.98660356409498	28.115367962584294	22.30134862343301
32	23.62097934884409	25.640834728254845	28.338307218462194	22.399878704438862
33	23.344804066830786	25.166301945173775	28.654282606473746	22.834611381521693
34	22.967808618347444	25.219842563957123	28.837906848348283	22.97444196934715
35	23.40109730065483	25.225404200084427	28.871671507433526	22.501826991827215
36	23.003536207830557	25.227028694206805	28.895046172861065	22.87438892510157
37	23.11685595407555	25.192891755218515	28.881249254030045	22.809003036675886
38	23.33650109687197	25.332192126212362	28.776503226764262	22.554803550151405
39	23.312291621964874	25.12559934244088	28.810843227517847	22.751265808076397
40	23.020683645788985	25.3367948595591	28.709955373792713	22.932566120859203
41	23.241050785973126	25.52294609229067	28.610883794746073	22.625119326990134
42	23.221579418922968	25.36852890099137	28.721936017945243	22.687955662140418
43	23.1869573879398	25.291805397336596	28.85282060688844	22.66841660783516
44	23.333173140162934	25.287315476081694	28.62349618661286	22.756015197142514
45	23.22614830864215	25.057269058418385	28.729099585776563	22.9874830471629
46	23.112501407330846	25.18657427807594	28.633953867525662	23.06697044706755
47	23.378670256798678	25.232567767915743	28.54909661204982	22.83966536323576
48	23.235805023702948	25.080880629238774	28.604995003552453	23.078319343505825
49	23.03594712181382	25.4354264714476	28.500429475633602	23.02819693110498
50	23.48830104764077	25.40234996612253	28.40236192420426	22.706987062032436
51	23.328694500117212	25.247515370083452	28.403614138423595	23.02017599137574
52	23.372172280309165	25.386612679311998	28.164542753413752	23.076672286965085
53	23.633332272899672	25.51874948247453	27.819845406821837	23.028072837803965
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	6934.0
1	4102.5
2	1271.0
3	984.5
4	698.0
5	687.0
6	676.0
7	779.0
8	1097.0
9	1312.0
10	1911.5
11	2511.0
12	3498.5
13	4486.0
14	5755.0
15	7024.0
16	9827.5
17	12631.0
18	16847.0
19	21063.0
20	30808.0
21	40553.0
22	176954.0
23	313355.0
24	219903.0
25	123424.0
26	120397.0
27	129669.0
28	138941.0
29	151683.0
30	164425.0
31	179948.0
32	195471.0
33	216873.5
34	238276.0
35	263920.0
36	289564.0
37	318121.0
38	346678.0
39	373796.0
40	400914.0
41	430192.0
42	487067.5
43	514665.0
44	544151.0
45	573637.0
46	591377.5
47	609118.0
48	622693.0
49	636268.0
50	638157.5
51	640047.0
52	629061.0
53	618075.0
54	594727.5
55	571380.0
56	539080.5
57	506781.0
58	458631.5
59	359871.0
60	309260.0
61	265982.5
62	222705.0
63	186202.0
64	149699.0
65	121170.0
66	92641.0
67	73578.0
68	54515.0
69	42132.0
70	29749.0
71	22635.0
72	15521.0
73	11673.0
74	7825.0
75	5832.0
76	2924.5
77	2010.0
78	1508.0
79	1006.0
80	811.5
81	617.0
82	449.5
83	282.0
84	187.0
85	92.0
86	71.0
87	50.0
88	40.5
89	31.0
90	21.0
91	11.0
92	8.0
93	3.0
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
53	8864298.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.27334614647043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.9207671564017	63.24644376051104
2	11.822524154618833	18.034869543404998
3	2.8839660346070177	6.599092188967343
4	1.046093893606635	3.1915632659507156
5	0.48118840102671595	1.8350924736588665
6	0.2512855645857515	1.1499834509556166
7	0.16153510861728518	0.8624576278061725
8	0.09616074101716507	0.5867601188242664
9	0.06623763465175216	0.4546949432144884
>10	0.26477853946125	3.113636831370728
>50	0.004031726689128781	0.20803751107392318
>100	0.001286343107763876	0.1512119739502812
>500	4.824990865791045E-5	0.02688632512048189
>1k	6.430114775928966E-5	0.08796153355235747
>5k	1.6075276321861996E-5	0.07985687745932878
>10k+	1.6075276321861996E-5	0.37145157417938973
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGAACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30295	0.34176423220428737	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.02496734654002E-5	1.1281209183175025E-5	0.0	0.0	0.0
2	6.430289234409764E-4	2.256241836635005E-5	0.0	0.0	0.0
3	6.430289234409764E-4	2.256241836635005E-5	0.0	0.0	3.3843627549525075E-5
4	6.994349693568515E-4	3.3843627549525075E-5	0.0	0.0	3.3843627549525075E-5
5	6.994349693568515E-4	5.640604591587512E-5	0.0	0.0	5.640604591587512E-5
6	7.332785969063766E-4	5.640604591587512E-5	0.0	0.0	7.896846428222517E-5
7	7.332785969063766E-4	5.640604591587512E-5	0.0	0.0	7.896846428222517E-5
8	7.784034336390767E-4	6.768725509905015E-5	0.0	0.0	9.02496734654002E-5
9	7.896846428222517E-4	6.768725509905015E-5	0.0	0.0	1.0153088264857521E-4
10	8.912155254708269E-4	6.768725509905015E-5	0.0	0.0	1.1281209183175024E-4
11	9.25059153020352E-4	6.768725509905015E-5	0.0	0.0	1.2409330101492526E-4
12	9.81465198936227E-4	7.896846428222517E-5	0.0	4.51248367327001E-5	1.353745101981003E-4
13	0.0010265900356689272	9.02496734654002E-5	0.0	1.0153088264857521E-4	1.353745101981003E-4
14	0.0011055584999511523	1.0153088264857521E-4	0.0	1.5793692856445033E-4	1.4665571938127532E-4
15	0.0011619645458670275	1.1281209183175024E-4	0.0	1.6921813774762535E-4	1.4665571938127532E-4
16	0.0012522142193324276	1.1281209183175024E-4	0.0	1.6921813774762535E-4	1.4665571938127532E-4
17	0.0013537451019810028	1.1281209183175024E-4	0.0	1.917805561139754E-4	1.4665571938127532E-4
18	0.001410151147896878	1.1281209183175024E-4	0.0	1.917805561139754E-4	1.804993469308004E-4
19	0.0015342444489118033	1.353745101981003E-4	0.0	1.917805561139754E-4	1.804993469308004E-4
20	0.0016583377499267285	1.353745101981003E-4	0.0	1.917805561139754E-4	1.804993469308004E-4
21	0.0018049934693080039	1.353745101981003E-4	0.0	1.917805561139754E-4	1.917805561139754E-4
22	0.0019403679795061042	1.6921813774762535E-4	0.0	1.917805561139754E-4	1.917805561139754E-4
23	0.0020306176529715043	1.6921813774762535E-4	0.0	1.917805561139754E-4	2.0306176529715043E-4
24	0.00222239820908548	1.6921813774762535E-4	0.0	1.917805561139754E-4	2.0306176529715043E-4
25	0.0025157096478480305	1.6921813774762535E-4	0.0	1.917805561139754E-4	2.1434297448032547E-4
26	0.002718771413145181	1.917805561139754E-4	0.0	1.917805561139754E-4	2.2562418366350048E-4
27	0.0029331143876255065	2.0306176529715043E-4	0.0	1.917805561139754E-4	2.481866020298505E-4
28	0.003260269453937582	2.2562418366350048E-4	0.0	1.917805561139754E-4	2.481866020298505E-4
29	0.003677674193715058	2.369053928466755E-4	0.0	1.917805561139754E-4	2.707490203962006E-4
30	0.004117641351858884	2.369053928466755E-4	0.0	1.917805561139754E-4	2.820302295793756E-4
31	0.00444479641817096	2.5946781121302556E-4	0.0	1.917805561139754E-4	2.820302295793756E-4
32	0.004749389066116685	2.820302295793756E-4	0.0	1.917805561139754E-4	2.9331143876255064E-4
33	0.005324730734458612	2.820302295793756E-4	0.0	1.917805561139754E-4	3.045926479457256E-4
34	0.005663167009953862	2.9331143876255064E-4	0.0	1.917805561139754E-4	3.1587385712890067E-4
35	0.0061144153772808634	3.045926479457256E-4	0.0	1.917805561139754E-4	3.271550663120757E-4
36	0.006509257698691989	3.045926479457256E-4	0.0	1.917805561139754E-4	3.384362754952507E-4
37	0.0069492248568358145	3.045926479457256E-4	0.0	1.917805561139754E-4	3.4971748467842574E-4
38	0.007298942341514241	3.045926479457256E-4	0.0	1.917805561139754E-4	3.722799030447758E-4
39	0.0077501907088412415	3.045926479457256E-4	0.0	2.0306176529715043E-4	3.722799030447758E-4
40	0.008212720285351418	3.045926479457256E-4	0.0	2.0306176529715043E-4	3.835611122279508E-4
41	0.008754218326143818	3.045926479457256E-4	0.0	2.0306176529715043E-4	4.0612353059430085E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	78320	0.0	44.098503	1
TCAACGC	79670	0.0	43.42795	12
CAACGCA	79715	0.0	43.41228	13
ATCAACG	80030	0.0	43.256092	11
GTGGTAT	79435	0.0	43.160007	6
AACGCAG	80305	0.0	43.084553	14
GTATCAA	79995	0.0	43.037064	9
AGTGGTA	79715	0.0	43.034935	5
ACGCAGA	80385	0.0	43.02998	15
GGTATCA	80085	0.0	42.944683	8
TATCAAC	80385	0.0	42.86627	10
TGGTATC	80025	0.0	42.862354	7
CGCAGAG	80920	0.0	42.754204	16
AGAGTAC	69120	0.0	42.59715	19
GAGTACT	32815	0.0	42.538475	20
AGCAGTG	81735	0.0	42.281887	2
GCAGTGG	81960	0.0	42.140007	3
GTACTTT	32795	0.0	42.098644	22
CAGTGGT	81680	0.0	42.051422	4
CAGAGTA	71325	0.0	41.48454	18
>>END_MODULE